LOCUS       RC223262                8370 bp    DNA     circular  SYN 
DEFINITION  ITGAX (Myc-DDK-tagged)-Human integrin, alpha X (complement component 3 receptor 4 subunit) (ITGAX)
ACCESSION   NM_000887
VERSION     NM_000887.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4527..4618 
                     /label="Myc-DDK"  
     misc_feature    4648..5233 
                     /label="PolyA signal"  
     misc_feature    4705..4724 
                     /label="Restriction Enzyme"  
     misc_feature    5509..6181 
                     /label="ColE1"  
     misc_feature    6611..7405 
                     /label="Kan/Neo"  
     misc_feature    7440..7742 
                     /label="SV40 ori"  
     misc_feature    7828..8305 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4509..4515 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4509 
                     /label="ITGAX"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACCAGGACC AGGGCAGCAC TCCTCCTGTT CACAGCCTTA GCAACTTCTC 
     1081 TAGGTTTCAA CTTGGACACA GAGGAGCTGA CAGCCTTCCG TGTGGACAGC GCTGGGTTTG 
     1141 GAGACAGCGT GGTCCAGTAT GCCAACTCCT GGGTGGTGGT TGGAGCCCCC CAAAAGATAA 
     1201 CAGCTGCCAA CCAAACGGGT GGCCTCTACC AGTGTGGCTA CAGCACTGGT GCCTGTGAGC 
     1261 CCATCGGCCT GCAGGTGCCC CCGGAGGCCG TGAACATGTC CCTGGGCCTG TCCCTGGCGT 
     1321 CTACCACCAG CCCTTCCCAG CTGCTGGCCT GCGGCCCCAC CGTGCACCAC GAGTGCGGGA 
     1381 GGAACATGTA CCTCACCGGA CTCTGCTTCC TCCTGGGCCC CACCCAGCTC ACCCAGAGGC 
     1441 TCCCGGTGTC CAGGCAGGAG TGCCCAAGAC AGGAGCAGGA CATTGTGTTC CTGATCGATG 
     1501 GCTCAGGCAG CATCTCCTCC CGCAACTTTG CCACGATGAT GAACTTCGTG AGAGCTGTGA 
     1561 TAAGCCAGTT CCAGAGACCC AGCACCCAGT TTTCCCTGAT GCAGTTCTCC AACAAATTCC 
     1621 AAACACACTT CACTTTCGAG GAATTCAGGC GCAGCTCAAA CCCCCTCAGC CTGTTGGCTT 
     1681 CTGTTCACCA GCTGCAAGGG TTTACATACA CGGCCACCGC CATCCAAAAT GTCGTGCACC 
     1741 GATTGTTCCA TGCCTCATAT GGGGCCCGTA GGGATGCCGC CAAAATTCTC ATTGTCATCA 
     1801 CTGATGGGAA GAAAGAAGGC GACAGCCTGG ATTATAAGGA TGTCATCCCC ATGGCTGATG 
     1861 CAGCAGGCAT CATCCGCTAT GCAATTGGGG TTGGATTAGC TTTTCAAAAC AGAAATTCTT 
     1921 GGAAAGAATT AAATGACATT GCATCGAAGC CCTCCCAGGA ACACATATTT AAAGTGGAGG 
     1981 ACTTTGATGC TCTGAAAGAT ATTCAAAACC AACTGAAGGA GAAGATCTTT GCCATTGAGG 
     2041 GTACGGAGAC CACAAGCAGT AGCTCCTTCG AATTGGAGAT GGCACAGGAG GGCTTCAGCG 
     2101 CTGTGTTCAC ACCTGATGGC CCCGTTCTGG GGGCTGTGGG GAGCTTCACC TGGTCTGGAG 
     2161 GTGCCTTCCT GTACCCCCCA AATATGAGCC CTACCTTCAT CAACATGTCT CAGGAGAATG 
     2221 TGGACATGAG GGACTCTTAC CTGGGTTACT CCACCGAGCT GGCCCTCTGG AAAGGGGTGC 
     2281 AGAGCCTGGT CCTGGGGGCC CCCCGCTACC AGCACACCGG GAAGGCTGTC ATCTTCACCC 
     2341 AGGTGTCCAG GCAATGGAGG ATGAAGGCCG AAGTCACGGG GACTCAGATC GGCTCCTACT 
     2401 TCGGGGCCTC CCTCTGCTCC GTGGACGTAG ACAGCGACGG CAGCACCGAC CTGGTCCTCA 
     2461 TCGGGGCCCC CCATTACTAC GAGCAGACCC GAGGGGGCCA GGTGTCTGTG TGTCCCTTGC 
     2521 CCAGGGGGTG GAGAAGGTGG TGGTGTGATG CTGTTCTCTA CGGGGAGCAG GGCCACCCCT 
     2581 GGGGTCGCTT TGGGGCGGCT CTGACAGTGC TGGGGGATGT GAATGGGGAC AAGCTGACAG 
     2641 ACGTGGTCAT CGGGGCCCCA GGAGAGGAGG AGAACCGGGG TGCTGTCTAC CTGTTTCACG 
     2701 GAGTCTTGGG ACCCAGCATC AGCCCCTCCC ACAGCCAGCG GATCGCGGGC TCCCAGCTCT 
     2761 CCTCCAGGCT GCAGTATTTT GGGCAGGCAC TGAGCGGGGG TCAAGACCTC ACCCAGGATG 
     2821 GACTGGTGGA CCTGGCTGTG GGGGCCCGGG GCCAGGTGCT CCTGCTCAGG ACCAGACCTG 
     2881 TGCTCTGGGT GGGGGTGAGC ATGCAGTTCA TACCTGCCGA GATCCCCAGG TCTGCGTTTG 
     2941 AGTGTCGGGA GCAGGTGGTC TCTGAGCAGA CCCTGGTACA GTCCAACATC TGCCTTTACA 
     3001 TTGACAAACG TTCTAAGAAC CTGCTTGGGA GCCGTGACCT CCAAAGCTCT GTGACCTTGG 
     3061 ACCTGGCCCT CGACCCTGGC CGCCTGAGTC CCCGTGCCAC CTTCCAGGAA ACAAAGAACC 
     3121 GGAGTCTGAG CCGAGTCCGA GTCCTCGGGC TGAAGGCACA CTGTGAAAAC TTCAACCTGC 
     3181 TGCTCCCGAG CTGCGTGGAG GACTCTGTGA CCCCCATTAC CTTGCGTCTG AACTTCACGC 
     3241 TGGTGGGCAA GCCCCTCCTT GCCTTCAGAA ACCTGCGGCC TATGCTGGCC GCCGATGCTC 
     3301 AGAGATACTT CACGGCCTCC CTACCCTTTG AGAAGAACTG TGGAGCCGAC CATATCTGCC 
     3361 AGGACAATCT CGGCATCTCC TTCAGCTTCC CAGGCTTGAA GTCCCTGCTG GTGGGGAGTA 
     3421 ACCTGGAGCT GAACGCAGAA GTGATGGTGT GGAATGACGG GGAAGACTCC TACGGAACCA 
     3481 CCATCACCTT CTCCCACCCC GCAGGACTGT CCTACCGCTA CGTGGCAGAG GGCCAGAAAC 
     3541 AAGGGCAGCT GCGTTCCCTG CACCTGACAT GTGACAGCGC CCCAGTTGGG AGCCAGGGCA 
     3601 CCTGGAGCAC CAGCTGCAGA ATCAACCACC TCATCTTCCG TGGCGGCGCC CAGATCACCT 
     3661 TCTTGGCTAC CTTTGACGTC TCCCCCAAGG CTGTCCTGGG AGACCGGCTG CTTCTGACAG 
     3721 CCAATGTGAG CAGTGAGAAC AACACTCCCA GGACCAGCAA GACCACCTTC CAGCTGGAGC 
     3781 TCCCGGTGAA GTATGCTGTC TACACTGTGG TTAGCAGCCA CGAACAATTC ACCAAATACC 
     3841 TCAACTTCTC AGAGTCTGAG GAGAAGGAAA GCCATGTGGC CATGCACAGA TACCAGGTCA 
     3901 ATAACCTGGG ACAGAGGGAC CTGCCTGTCA GCATCAACTT CTGGGTGCCT GTGGAGCTGA 
     3961 ACCAGGAGGC TGTGTGGATG GATGTGGAGG TCTCCCACCC CCAGAACCCA TCCCTTCGGT 
     4021 GCTCCTCAGA GAAAATCGCA CCCCCAGCAT CTGACTTCCT GGCGCACATT CAGAAGAATC 
     4081 CCGTGCTGGA CTGCTCCATT GCTGGCTGCC TGCGGTTCCG CTGTGACGTC CCCTCCTTCA 
     4141 GCGTCCAGGA GGAGCTGGAT TTCACCCTGA AGGGCAACCT CAGCTTTGGC TGGGTCCGCC 
     4201 AGATATTGCA GAAGAAGGTG TCGGTCGTGA GTGTGGCTGA AATTACGTTC GACACATCCG 
     4261 TGTACTCCCA GCTTCCAGGA CAGGAGGCAT TTATGAGAGC TCAGACGACA ACGGTGCTGG 
     4321 AGAAGTACAA GGTCCACAAC CCCACCCCCC TCATCGTAGG CAGCTCCATT GGGGGTCTGT 
     4381 TGCTGCTGGC ACTCATCACA GCGGTACTGT ACAAAGTTGG CTTCTTCAAG CGTCAGTACA 
     4441 AGGAAATGAT GGAGGAGGCA AATGGACAAA TTGCCCCAGA AAACGGGACA CAGACCCCCA 
     4501 GCCCGCCCAG TGAGAAAACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4561 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4621 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4681 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4741 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4801 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4861 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4921 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4981 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5041 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5101 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5161 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5221 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5281 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5341 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5401 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5461 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5521 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5581 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5641 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5701 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5761 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5821 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5881 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5941 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6001 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6061 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6121 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6181 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6241 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6301 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6361 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6421 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6481 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6541 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6601 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6661 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6721 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6781 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6841 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6901 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6961 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7021 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7081 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7141 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7201 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7261 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7321 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7381 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7441 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7501 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7561 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7621 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7681 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7741 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7801 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7861 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7921 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7981 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8041 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8101 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8161 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8221 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8281 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8341 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//