LOCUS       RC223212                7647 bp    DNA     circular  SYN 
DEFINITION  NLRP3 (Myc-DDK-tagged)-Human NLR family, pyrin domain containing 3 (NLRP3), transcript variant 2
ACCESSION   NM_183395
VERSION     NM_183395.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3804..3895 
                     /label="Myc-DDK"  
     misc_feature    3925..4510 
                     /label="PolyA signal"  
     misc_feature    3982..4001 
                     /label="Restriction Enzyme"  
     misc_feature    4786..5458 
                     /label="ColE1"  
     misc_feature    5888..6682 
                     /label="Kan/Neo"  
     misc_feature    6717..7019 
                     /label="SV40 ori"  
     misc_feature    7105..7582 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3786..3792 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3786 
                     /label="NLRP3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGATGGCA AGCACCCGCT GCAAGCTGGC CAGGTACCTG GAGGACCTGG 
     1081 AGGATGTGGA CTTGAAGAAA TTTAAGATGC ACTTAGAGGA CTATCCTCCC CAGAAGGGCT 
     1141 GCATCCCCCT CCCGAGGGGT CAGACAGAGA AGGCAGACCA TGTGGATCTA GCCACGCTAA 
     1201 TGATCGACTT CAATGGGGAG GAGAAGGCGT GGGCCATGGC CGTGTGGATC TTCGCTGCGA 
     1261 TCAACAGGAG AGACCTTTAT GAGAAAGCAA AAAGAGATGA GCCGAAGTGG GGTTCAGATA 
     1321 ATGCACGTGT TTCGAATCCC ACTGTGATAT GCCAGGAAGA CAGCATTGAA GAGGAGTGGA 
     1381 TGGGTTTACT GGAGTACCTT TCGAGAATCT CTATTTGTAA AATGAAGAAA GATTACCGTA 
     1441 AGAAGTACAG AAAGTACGTG AGAAGCAGAT TCCAGTGCAT TGAAGACAGG AATGCCCGTC 
     1501 TGGGTGAGAG TGTGAGCCTC AACAAACGCT ACACACGACT GCGTCTCATC AAGGAGCACC 
     1561 GGAGCCAGCA GGAGAGGGAG CAGGAGCTTC TGGCCATCGG CAAGACCAAG ACGTGTGAGA 
     1621 GCCCCGTGAG TCCCATTAAG ATGGAGTTGC TGTTTGACCC CGATGATGAG CATTCTGAGC 
     1681 CTGTGCACAC CGTGGTGTTC CAGGGGGCGG CAGGGATTGG GAAAACAATC CTGGCCAGGA 
     1741 AGATGATGTT GGACTGGGCG TCGGGGACAC TCTACCAAGA CAGGTTTGAC TATCTGTTCT 
     1801 ATATCCACTG TCGGGAGGTG AGCCTTGTGA CACAGAGGAG CCTGGGGGAC CTGATCATGA 
     1861 GCTGCTGCCC CGACCCAAAC CCACCCATCC ACAAGATCGT GAGAAAACCC TCCAGAATCC 
     1921 TCTTCCTCAT GGACGGCTTC GATGAGCTGC AAGGTGCCTT TGACGAGCAC ATAGGACCGC 
     1981 TCTGCACTGA CTGGCAGAAG GCCGAGCGGG GAGACATTCT CCTGAGCAGC CTCATCAGAA 
     2041 AGAAGCTGCT TCCCGAGGCC TCTCTGCTCA TCACCACGAG ACCTGTGGCC CTGGAGAAAC 
     2101 TGCAGCACTT GCTGGACCAT CCTCGGCATG TGGAGATCCT GGGTTTCTCC GAGGCCAAAA 
     2161 GGAAAGAGTA CTTCTTCAAG TACTTCTCTG ATGAGGCCCA AGCCAGGGCA GCCTTCAGTC 
     2221 TGATTCAGGA GAACGAGGTC CTCTTCACCA TGTGCTTCAT CCCCCTGGTC TGCTGGATCG 
     2281 TGTGCACTGG ACTGAAACAG CAGATGGAGA GTGGCAAGAG CCTTGCCCAG ACATCCAAGA 
     2341 CCACCACCGC GGTGTACGTC TTCTTCCTTT CCAGTTTGCT GCAGCCCCGG GGAGGGAGCC 
     2401 AGGAGCACGG CCTCTGCGCC CACCTCTGGG GGCTCTGCTC TTTGGCTGCA GATGGAATCT 
     2461 GGAACCAGAA AATCCTGTTT GAGGAGTCCG ACCTCAGGAA TCATGGACTG CAGAAGGCGG 
     2521 ATGTGTCTGC TTTCCTGAGG ATGAACCTGT TCCAAAAGGA AGTGGACTGC GAGAAGTTCT 
     2581 ACAGCTTCAT CCACATGACT TTCCAGGAGT TCTTTGCCGC CATGTACTAC CTGCTGGAAG 
     2641 AGGAAAAGGA AGGAAGGACG AACGTTCCAG GGAGTCGTTT GAAGCTTCCC AGCCGAGACG 
     2701 TGACAGTCCT TCTGGAAAAC TATGGCAAAT TCGAAAAGGG GTATTTGATT TTTGTTGTAC 
     2761 GTTTCCTCTT TGGCCTGGTA AACCAGGAGA GGACCTCCTA CTTGGAGAAG AAATTAAGTT 
     2821 GCAAGATCTC TCAGCAAATC AGGCTGGAGC TGCTGAAATG GATTGAAGTG AAAGCCAAAG 
     2881 CTAAAAAGCT GCAGATCCAG CCCAGCCAGC TGGAATTGTT CTACTGTTTG TACGAGATGC 
     2941 AGGAGGAGGA CTTCGTGCAA AGGGCCATGG ACTATTTCCC CAAGATTGAG ATCAATCTCT 
     3001 CCACCAGAAT GGACCACATG GTTTCTTCCT TTTGCATTGA GAACTGTCAT CGGGTGGAGT 
     3061 CACTGTCCCT GGGGTTTCTC CATAACATGC CCAAGGAGGA AGAGGAGGAG GAAAAGGAAG 
     3121 GCCGACACCT TGATATGGTG CAGTGTGTCC TCCCAAGCTC CTCTCATGCT GCCTGTTCTC 
     3181 ATGGGTTGGG GCGCTGTGGC CTCTCGCATG AGTGCTGCTT CGACATCTCC TTGGTCCTCA 
     3241 GCAGCAACCA GAAGCTGGTG GAGCTGGACC TGAGTGACAA CGCCCTCGGT GACTTCGGAA 
     3301 TCAGACTTCT GTGTGTGGGA CTGAAGCACC TGTTGTGCAA TCTGAAGAAG CTCTGGTTGG 
     3361 TGAATTCTGG CCTTACGTCA GTCTGTTGTT CAGCTTTGTC CTCGGTACTC AGCACTAATC 
     3421 AGAATCTCAC GCACCTTTAC CTGCGAGGCA ACACTCTCGG AGACAAGGGG ATCAAACTAC 
     3481 TCTGTGAGGG ACTCTTGCAC CCCGACTGCA AGCTTCAGGT GTTGGAATTA GACAACTGCA 
     3541 ACCTCACGTC ACACTGCTGC TGGGATCTTT CCACACTTCT GACCTCCAGC CAGAGCCTGC 
     3601 GAAAGCTGAG CCTGGGCAAC AATGACCTGG GCGACCTGGG GGTCATGATG TTCTGTGAAG 
     3661 TGCTGAAACA GCAGAGCTGC CTCCTGCAGA ACCTGGGGTT GTCTGAAATG TATTTCAATT 
     3721 ATGAGACAAA AAGTGCGTTA GAAACACTTC AAGAAGAAAA GCCTGAGCTG ACCGTCGTCT 
     3781 TTGAGCCTTC TTGGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     3841 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     3901 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     3961 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4021 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4081 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4141 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4201 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4261 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4321 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4381 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     4441 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     4501 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     4561 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     4621 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     4681 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     4741 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     4801 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     4861 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     4921 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     4981 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5041 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5101 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5161 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     5221 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     5281 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     5341 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     5401 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     5461 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     5521 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     5581 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     5641 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     5701 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     5761 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     5821 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     5881 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     5941 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6001 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6061 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6121 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6181 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6241 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     6301 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     6361 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     6421 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     6481 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     6541 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     6601 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     6661 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     6721 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     6781 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     6841 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     6901 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     6961 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7021 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7081 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7141 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7201 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7261 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     7321 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     7381 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     7441 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     7501 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     7561 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     7621 ATTTAACAAA AATTTAACGC GAATTTT
//