LOCUS       RC223198                7725 bp    DNA     circular  SYN 
DEFINITION  UNC5D (Myc-DDK-tagged)-Human unc-5 homolog D (C. elegans) (UNC5D)
ACCESSION   NM_080872
VERSION     NM_080872.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3882..3973 
                     /label="Myc-DDK"  
     misc_feature    4003..4588 
                     /label="PolyA signal"  
     misc_feature    4060..4079 
                     /label="Restriction Enzyme"  
     misc_feature    4864..5536 
                     /label="ColE1"  
     misc_feature    5966..6760 
                     /label="Kan/Neo"  
     misc_feature    6795..7097 
                     /label="SV40 ori"  
     misc_feature    7183..7660 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3864..3870 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3864 
                     /label="UNC5D"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATCCTGGTG TTAGTTAAAG CTCTCAGTGA TGTCTGTGCT GGGACTTCCG 
     1081 GGTTCCTGTT GGACTTTTCC TCCCAAACTT CACCAGGAAC TGACAATGGC GAAGCCCTTC 
     1141 CCGAATCCAT CCCATCAGCT CCTGGGACAC TGCCTCATTT CATAGAGGAG CCAGATGATG 
     1201 CTTATATTAT CAAGAGCAAC CCTATTGCAC TCAGGTGCAA AGCGAGGCCA GCCATGCAGA 
     1261 TATTCTTCAA ATGCAACGGC GAGTGGGTCC ATCAGAACGA GCACGTCTCT GAAGAGACTC 
     1321 TGGACGAGAG CTCAGGTTTG AAGGTCCGCG AAGTGTTCAT CAATGTTACT AGGCAACAGG 
     1381 TGGAGGACTT CCATGGGCCC GAGGACTATT GGTGCCAGTG TGTGGCGTGG AGCCACCTGG 
     1441 GTACCTCCAA GAGCAGGAAG GCCTCTGTGC GCATAGCCTA TTTACGGAAA AACTTTGAAC 
     1501 AAGACCCACA AGGAAGGGAA GTTCCCATTG AAGGCATGAT TGTACTGCAC TGCCGCCCAC 
     1561 CAGAGGGAGT CCCTGCTGCC GAGGTGGAAT GGCTGAAAAA TGAAGAGCCC ATTGACTCTG 
     1621 AACAAGACGA GAACATTGAC ACCAGGGCTG ACCATAACCT GATCATCAGG CAGGCACGGC 
     1681 TCTCGGACTC AGGAAATTAC ACCTGCATGG CAGCCAACAT CGTGGCTAAG AGGAGAAGCC 
     1741 TGTCGGCCAC TGTTGTGGTC TACGTGAATG GAGGCTGGTC TTCCTGGACA GAGTGGTCAG 
     1801 CCTGCAATGT TCGCTGTGGT AGAGGATGGC AGAAACGTTC CCGGACCTGC ACCAACCCAG 
     1861 CTCCTCTCAA TGGTGGGGCC TTTTGTGAGG GAATGTCAGT GCAGAAAATA ACCTGCACTT 
     1921 CTCTTTGTCC TGTGGATGGG AGCTGGGAAG TGTGGAGCGA ATGGTCCGTC TGCAGTCCAG 
     1981 AGTGTGAACA TTTGCGGATC CGGGAGTGCA CAGCACCACC CCCGAGAAAT GGGGGCAAAT 
     2041 TCTGTGAAGG TCTAAGCCAG GAATCTGAAA ACTGCACAGA TGGTCTTTGC ATCCTAGATA 
     2101 AAAAACCTCT TCATGAAATA AAACCCCAAA GCATTGAGAA TGCCAGCGAC ATTGCTTTGT 
     2161 ACTCGGGCTT GGGTGCTGCC GTCGTGGCCG TTGCAGTCCT GGTCATTGGT GTCACCCTTT 
     2221 ACAGACGGAG CCAGAGTGAC TATGGCGTGG ACGTCATTGA CTCTTCTGCA TTGACAGGTG 
     2281 GCTTCCAGAC CTTCAACTTC AAAACAGTCC GTCAAGGTAA CTCCCTGCTC CTGAATTCTG 
     2341 CCATGCAGCC AGATCTGACA GTGAGCCGGA CATACAGCGG ACCCATCTGT CTGCAGGACC 
     2401 CTCTGGACAA GGAGCTCATG ACAGAGTCCT CACTCTTTAA CCCTTTGTCG GACATCAAAG 
     2461 TGAAAGTCCA GAGCTCGTTC ATGGTTTCCC TGGGAGTGTC TGAGAGAGCT GAGTACCACG 
     2521 GCAAGAATCA TTCCAGGACT TTTCCCCATG GAAACAACCA CAGCTTTAGT ACAATGCATC 
     2581 CCAGAAATAA AATGCCCTAC ATCCAAAATC TGTCATCACT CCCCACAAGG ACAGAACTGA 
     2641 GGACAACTGG TGTCTTTGGC CATTTAGGGG GGCGCTTAGT AATGCCAAAT ACAGGGGTGA 
     2701 GCTTACTCAT ACCACACGGT GCCATCCCAG AGGAGAATTC TTGGGAGATT TATATGTCCA 
     2761 TCAACCAAGG TGAACCCAGC CTCCAGTCAG ATGGCTCTGA GGTGCTCCTG AGTCCTGAAG 
     2821 TCACCTGTGG TCCTCCAGAC ATGATCGTCA CCACTCCCTT TGCATTGACC ATCCCGCACT 
     2881 GTGCAGATGT CAGTTCTGAG CATTGGAATA TCCATTTAAG GAAGAGGACA CAGCAGGGCA 
     2941 AATGGGAGGA AGTGATGTCA GTGGAAGATG AATCTACATC CTGTTACTGC CTTTTGGACC 
     3001 CCTTTGCGTG TCATGTGCTC CTGGACAGCT TTGGGACCTA TGCGCTCACT GGAGAGCCAA 
     3061 TCACAGACTG TGCCGTGAAG CAACTGAAGG TGGCGGTTTT TGGCTGCATG TCCTGTAACT 
     3121 CCCTGGATTA CAACTTGAGA GTTTACTGTG TGGACAATAC CCCTTGTGCA TTTCAGGAAG 
     3181 TGGTTTCAGA TGAAAGGCAT CAAGGTGGAC AGCTCCTGGA AGAACCAAAA TTGCTGCATT 
     3241 TCAAAGGGAA TACCTTTAGT CTTCAGATTT CTGTCCTTGA TATTCCCCCA TTCCTCTGGA 
     3301 GAATTAAACC ATTCACTGCC TGCCAGGAAG TCCCGTTCTC CCGCGTGTGG TGCAGTAACC 
     3361 GGCAGCCCCT GCACTGTGCC TTCTCCCTGG AGCGTTATAC GCCCACTACC ACCCAGCTGT 
     3421 CCTGCAAAAT CTGCATTCGG CAGCTCAAAG GCCATGAACA GATCCTCCAA GTGCAGACAT 
     3481 CAATCCTAGA GAGTGAACGA GAAACCATCA CTTTCTTCGC ACAAGAGGAC AGCACTTTCC 
     3541 CTGCACAGAC TGGCCCCAAA GCCTTCAAAA TTCCCTACTC CATCAGACAG CGGATTTGTG 
     3601 CTACATTTGA TACCCCCAAT GCCAAAGGCA AGGACTGGCA GATGTTAGCA CAGAAAAACA 
     3661 GCATCAACAG GAATTTATCT TATTTCGCTA CACAAAGTAG CCCATCTGCT GTCATTTTGA 
     3721 ACCTGTGGGA AGCTCGTCAT CAGCATGATG GTGATCTTGA CTCCCTGGCC TGTGCCCTTG 
     3781 AAGAGATTGG GAGGACACAC ACGAAACTCT CAAACATTTC AGAATCCCAG CTTGATGAAG 
     3841 CCGACTTCAA CTACAGCAGG CAAAATGGAC TCACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     3901 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     3961 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4021 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4081 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4141 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4201 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4261 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4321 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4381 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4441 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4501 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     4561 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     4621 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     4681 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     4741 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     4801 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     4861 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     4921 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     4981 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5041 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5101 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5161 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5221 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5281 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5341 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5401 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     5461 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     5521 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     5581 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     5641 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     5701 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     5761 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     5821 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     5881 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     5941 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6001 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6061 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6121 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6181 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6241 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6301 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6361 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6421 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     6481 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     6541 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     6601 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     6661 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     6721 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     6781 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     6841 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     6901 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     6961 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7021 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7081 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7141 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7201 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7261 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7321 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7381 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7441 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     7501 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     7561 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     7621 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     7681 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//