LOCUS       RC223162                8400 bp    DNA     circular  SYN 
DEFINITION  ATP2B3 (Myc-DDK-tagged)-Human ATPase, Ca++ transporting, plasma membrane 3 (ATP2B3), transcript variant 1
ACCESSION   NM_021949
VERSION     NM_021949.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4557..4648 
                     /label="Myc-DDK"  
     misc_feature    4678..5263 
                     /label="PolyA signal"  
     misc_feature    4735..4754 
                     /label="Restriction Enzyme"  
     misc_feature    5539..6211 
                     /label="ColE1"  
     misc_feature    6641..7435 
                     /label="Kan/Neo"  
     misc_feature    7470..7772 
                     /label="SV40 ori"  
     misc_feature    7858..8335 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4539..4545 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4539 
                     /label="ATP2B3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCGACATG GCCAATAGTT CCATCGAGTT CCACCCCAAG CCCCAGCAGC 
     1081 AGCGGGATGT CCCCCAGGCT GGAGGCTTTG GGTGCACGCT GGCGGAGCTG CGCACCCTCA 
     1141 TGGAGCTGCG AGGGGCCGAG GCGCTGCAGA AGATCGAGGA GGCCTACGGG GATGTCAGCG 
     1201 GGCTCTGCCG GAGGCTGAAG ACCTCACCCA CAGAGGGCCT GGCGGACAAC ACCAATGACC 
     1261 TGGAGAAGCG CAGGCAGATC TACGGGCAGA ACTTCATCCC CCCAAAGCAA CCCAAGACCT 
     1321 TCCTGCAGCT GGTGTGGGAG GCCCTGCAGG ACGTGACCCT CATCATCCTG GAGGTGGCTG 
     1381 CCATCGTCTC TCTGGGCCTC TCGTTCTATG CGCCGCCAGG AGAGGAGAGT GAAGCCTGTG 
     1441 GGAATGTGTC GGGAGGCGCA GAAGATGAGG GCGAGGCCGA AGCTGGCTGG ATCGAGGGGG 
     1501 CTGCCATCCT GCTGTCCGTC ATCTGTGTGG TGCTGGTCAC GGCCTTCAAT GACTGGAGCA 
     1561 AGGAGAAGCA GTTCCGAGGC CTGCAGAGCC GAATTGAGCA GGAGCAGAAG TTCACGGTCA 
     1621 TCCGGAACGG GCAGCTCCTC CAGGTCCCCG TGGCTGCGCT GGTGGTGGGG GACATTGCCC 
     1681 AGGTCAAGTA CGGCGACCTG CTGCCAGCCG ACGGCGTGCT CATCCAGGCC AATGACCTCA 
     1741 AGATCGACGA GAGCTCCCTG ACGGGCGAGT CTGACCACGT GCGCAAGTCA GCTGACAAAG 
     1801 ATCCCATGCT GCTCTCAGGC ACTCATGTCA TGGAAGGTTC TGGAAGAATG GTGGTGACCG 
     1861 CCGTTGGCGT GAATTCCCAG ACAGGCATCA TCTTCACGCT GCTTGGAGCT GGCGGAGAGG 
     1921 AGGAAGAGAA GAAAGATAAG AAAGGCAAGC AGCAGGATGG GGCCATGGAG AGTAGCCAGA 
     1981 CCAAAGCTAA GAAGCAGGAT GGTGCAGTGG CCATGGAGAT GCAGCCCCTG AAGAGCGCGG 
     2041 AGGGTGGGGA GATGGAGGAG CGGGAGAAGA AGAAAGCCAA CGCACCCAAA AAGGAGAAGT 
     2101 CTGTCCTTCA GGGGAAGCTC ACAAAGCTAG CCGTGCAGAT CGGGAAAGCA GGGCTGGTGA 
     2161 TGTCTGCCAT CACCGTCATC ATCCTGGTCC TCTACTTTGT GATTGAGACG TTTGTCGTGG 
     2221 AAGGCCGGAC ATGGCTGGCA GAGTGCACGC CGGTCTATGT ACAATACTTC GTGAAGTTCT 
     2281 TCATCATTGG TGTCACTGTG CTGGTCGTGG CTGTCCCAGA GGGCCTGCCT CTTGCTGTCA 
     2341 CCATCTCCTT AGCTTACTCT GTCAAGAAAA TGATGAAAGA CAACAACCTG GTGCGCCACC 
     2401 TGGATGCCTG CGAGACCATG GGCAACGCCA CAGCCATCTG CTCCGACAAG ACGGGCACGC 
     2461 TCACCACCAA CCGTATGACC GTGGTCCAGT CCTACCTAGG AGACACCCAC TACAAAGAGA 
     2521 TTCCGGCCCC CAGCGCCCTG ACCCCTAAGA TCCTCGACCT CCTGGTCCAT GCCATCTCCA 
     2581 TCAACAGTGC CTATACCACC AAAATACTAC CTCCTGAGAA GGAAGGCGCC CTCCCACGCC 
     2641 AGGTGGGCAA TAAGACGGAG TGCGCCCTGC TGGGCTTCGT CTTGGACCTG AAGCGGGACT 
     2701 TCCAGCCCGT GCGCGAGCAG ATCCCGGAAG ACAAGCTTTA CAAAGTGTAC ACCTTCAACT 
     2761 CGGTCCGCAA GTCCATGAGC ACAGTCATCC GCATGCCCGA CGGTGGCTTC CGCCTCTTCA 
     2821 GCAAGGGGGC CTCAGAGATC CTCTTGAAAA AGTGCACCAA CATCTTGAAC AGCAATGGCG 
     2881 AACTCCGGGG CTTTCGGCCT CGGGACCGGG ACGACATGGT GAGGAAGATC ATCGAGCCGA 
     2941 TGGCTTGCGA TGGCCTCCGC ACCATCTGCA TCGCCTACCG GGACTTCTCT GCAGGCCAGG 
     3001 AGCCCGACTG GGACAACGAG AATGAGGTCG TGGGTGACCT CACCTGCATA GCTGTCGTGG 
     3061 GCATTGAGGA CCCTGTGCGG CCCGAGGTCC CTGAAGCTAT CCGAAAATGC CAGCGTGCTG 
     3121 GCATCACAGT CCGCATGGTG ACTGGGGACA ACATCAACAC GGCCCGGGCC ATCGCAGCCA 
     3181 AATGCGGCAT CATCCAGCCC GGGGAGGACT TCCTGTGCCT AGAAGGGAAG GAGTTCAACC 
     3241 GGCGGATCCG CAATGAGAAA GGCGAGATAG AACAGGAGCG GCTGGACAAG GTGTGGCCCA 
     3301 AGCTGAGGGT GCTGGCCCGG TCGTCTCCCA CCGACAAGCA CACACTGGTC AAAGGGATTA 
     3361 TCGACAGCAC CACTGGTGAG CAGCGGCAGG TGGTGGCTGT GACAGGGGAT GGCACCAACG 
     3421 ATGGGCCGGC CCTCAAGAAG GCGGACGTGG GCTTCGCCAT GGGCATCGCA GGGACCGACG 
     3481 TGGCCAAGGA GGCCTCCGAC ATCATCCTGA CCGATGACAA CTTCACCAGC ATCGTCAAGG 
     3541 CAGTCATGTG GGGCCGTAAC GTCTATGACA GCATCTCCAA GTTCCTGCAG TTTCAACTGA 
     3601 CGGTCAATGT GGTGGCTGTG ATCGTGGCCT TCACAGGTGC CTGCATTACT CAGGACTCTC 
     3661 CTCTCAAAGC CGTGCAGATG TTGTGGGTGA ACTTGATCAT GGACACATTT GCCTCTCTGG 
     3721 CCCTGGCGAC GGAGCCACCC ACAGAGTCGC TGCTGCTGCG GAAGCCGTAC GGCCGCGACA 
     3781 AGCCCCTCAT CTCCCGCACC ATGATGAAGA ACATTCTGGG CCACGCCGTG TACCAGCTCG 
     3841 CCATCATCTT CACCCTGCTG TTTGTCGGGG AGCTCTTCTT CGACATCGAC AGCGGGAGGA 
     3901 ATGCGCCCCT GCACTCGCCA CCCTCAGAGC ACTACACCAT CATCTTCAAC ACGTTCGTCA 
     3961 TGATGCAGCT CTTTAACGAG ATCAACGCCC GCAAGATCCA CGGCGAGAGG AACGTGTTCG 
     4021 ACGGCATCTT CAGCAACCCC ATCTTCTGCA CCATCGTTTT GGGCACTTTC GGGATTCAGA 
     4081 TTGTCATCGT CCAGTTTGGC GGGAAGCCCT TCAGCTGCTC CCCACTATCC ACAGAACAGT 
     4141 GGCTCTGGTG CCTGTTTGTT GGTGTTGGGG AGCTGGTCTG GGGACAGGTC ATTGCCACCA 
     4201 TCCCCACCAG CCAGCTCAAG TGCCTGAAGG AAGCCGGGCA CGGGCCCGGG AAGGACGAGA 
     4261 TGACCGACGA GGAGCTGGCC GAAGGCGAGG AAGAGATCGA CCATGCCGAG CGGGAGCTCC 
     4321 GCAGGGGCCA GATCCTCTGG TTCCGGGGCC TGAACCGGAT TCAGACGCAG ATGGAGGTAG 
     4381 TGAGTACCTT CAAGAGAAGC GGTTCAGTTC AGGGTGCTGT GCGCCGGCGG TCTTCGGTCC 
     4441 TCAGCCAGCT TCATGACGTA ACCAATCTTT CTACCCCTAC TCACGCAATT CTCTCTGCTG 
     4501 CCAATCCTAC CAGTGCTGCT GGGAATCCGG GTGGTGAAAG CGTTCCGACG CGTACGCGGC 
     4561 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4621 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4681 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4741 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4801 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4861 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4921 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4981 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5041 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5101 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5161 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5221 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5281 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5341 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5401 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5461 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5521 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5581 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5641 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5701 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5761 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5821 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5881 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5941 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6001 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6061 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6121 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6181 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6241 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6301 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6361 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6421 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6481 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6541 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6601 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6661 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6721 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6781 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6841 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6901 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6961 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7021 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7081 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7141 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7201 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7261 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7321 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7381 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7441 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7501 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7561 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7621 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7681 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7741 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7801 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7861 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7921 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7981 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     8041 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8101 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8161 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8221 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8281 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8341 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//