LOCUS       RC223155                8010 bp    DNA     circular  SYN 
DEFINITION  TJP2 (Myc-DDK-tagged)-Human tight junction protein 2 (zona occludens 2) (TJP2), transcript variant 2
ACCESSION   NM_201629
VERSION     NM_201629.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4167..4258 
                     /label="Myc-DDK"  
     misc_feature    4288..4873 
                     /label="PolyA signal"  
     misc_feature    4345..4364 
                     /label="Restriction Enzyme"  
     misc_feature    5149..5821 
                     /label="ColE1"  
     misc_feature    6251..7045 
                     /label="Kan/Neo"  
     misc_feature    7080..7382 
                     /label="SV40 ori"  
     misc_feature    7468..7945 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4149..4155 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4149 
                     /label="TJP2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGGTGCGA GGAGACCGCG GGTTTCCACC CCGGCGGGAG CTGTCAGGTT 
     1081 GGCTCCGCGC CCCAGGCATG GAAGAGCTGA TATGGGAACA GTACACTGTG ACCCTACAAA 
     1141 AGGATTCCAA AAGAGGATTT GGAATTGCAG TGTCCGGAGG CAGAGACAAC CCCCACTTTG 
     1201 AAAATGGAGA AACGTCAATT GTCATTTCTG ATGTGCTCCC GGGTGGGCCT GCTGATGGGC 
     1261 TGCTCCAAGA AAATGACAGA GTGGTCATGG TCAATGGCAC CCCCATGGAG GATGTGCTTC 
     1321 ATTCGTTTGC AGTTCAGCAG CTCAGAAAAA GTGGGAAGGT CGCTGCTATT GTGGTCAAGA 
     1381 GGCCCCGGAA GGTCCAGGTG GCCGCACTTC AGGCCAGCCC TCCCCTGGAT CAGGATGACC 
     1441 GGGCTTTTGA GGTGATGGAC GAGTTTGATG GCAGAAGTTT CCGGAGTGGC TACAGCGAGA 
     1501 GGAGCCGGCT GAACAGCCAT GGGGGGCGCA GCCGCAGCTG GGAGGACAGC CCGGAAAGGG 
     1561 GGCGTCCCCA TGAGCGGGCC CGGAGCCGGG AGCGGGACCT CAGCCGGGAC CGGAGCCGTG 
     1621 GCCGGAGCCT GGAGCGGGGC CTGGACCAAG ACCATGCGCG CACCCGAGAC CGCAGCCGTG 
     1681 GCCGGAGCCT GGAGCGGGGC CTGGACCACG ACTTTGGGCC ATCCCGGGAC CGGGACCGTG 
     1741 ACCGCAGCCG CGGCCGGAGC ATTGACCAGG ACTACGAGCG AGCCTATCAC CGGGCCTACG 
     1801 ACCCAGACTA CGAGCGGGCC TACAGCCCGG AGTACAGGCG CGGGGCCCGC CACGATGCCC 
     1861 GCTCTCGGGG ACCCCGAAGC CGCAGCCGCG AGCACCCGCA CTCACGGAGC CCCAGCCCCG 
     1921 AGCCTAGGGG GCGGCCGGGG CCCATCGGGG TCCTCCTGAT GAAAAGCAGA GCGAACGAAG 
     1981 AGTATGGTCT CCGGCTTGGG AGTCAGATCT TCGTAAAGGA AATGACCCGA ACGGGTCTGG 
     2041 CAACTAAAGA TGGCAACCTT CACGAAGGAG ACATAATTCT CAAGATCAAT GGGACTGTAA 
     2101 CTGAGAACAT GTCTTTAACG GATGCTCGAA AATTGATAGA AAAGTCAAGA GGAAAACTAC 
     2161 AGCTAGTGGT GTTGAGAGAC AGCCAGCAGA CCCTCATCAA CATCCCGTCA TTAAATGACA 
     2221 GTGACTCAGA AATAGAAGAT ATTTCAGAAA TAGAGTCAAA CCGATCATTT TCTCCAGAGG 
     2281 AGAGACGTCA TCAGTATTCT GATTATGATT ATCATTCCTC AAGTGAGAAG CTGAAGGAAA 
     2341 GGCCAAGTTC CAGAGAGGAC ACGCCGAGCA GATTGTCCAG GATGGGTGCG ACACCCACTC 
     2401 CCTTTAAGTC CACAGGGGAT ATTGCAGGCA CAGTTGTCCC AGAGACCAAC AAGGAACCCA 
     2461 GATACCAAGA GGACCCCCCA GCTCCTCAAC CAAAAGCAGC CCCGAGAACT TTTCTTCGTC 
     2521 CTAGTCCTGA AGATGAAGCA ATATATGGCC CTAATACCAA AATGGTAAGG TTCAAGAAGG 
     2581 GAGACAGCGT GGGCCTCCGG TTGGCTGGTG GCAATGATGT CGGGATATTT GTTGCTGGCA 
     2641 TTCAAGAAGG GACCTCGGCG GAGCAGGAGG GCCTTCAAGA AGGAGACCAG ATTCTGAAGG 
     2701 TGAACACACA GGATTTCAGA GGATTAGTGC GGGAGGATGC CGTTCTCTAC CTGTTAGAAA 
     2761 TCCCTAAAGG TGAAATGGTG ACCATTTTAG CTCAGAGCCG AGCCGATGTG TATAGAGACA 
     2821 TCCTGGCTTG TGGCAGAGGG GATTCGTTTT TTATAAGAAG CCACTTTGAA TGTGAGAAGG 
     2881 AAACTCCACA GAGCCTGGCC TTCACCAGAG GGGAGGTCTT CCGAGTGGTA GACACACTGT 
     2941 ATGACGGCAA GCTGGGCAAC TGGCTGGCTG TGAGGATTGG GAACGAGTTG GAGAAAGGCT 
     3001 TAATCCCCAA CAAGAGCAGA GCTGAACAAA TGGCCAGTGT TCAAAATGCC CAGAGAGACA 
     3061 ACGCTGGGGA CCGGGCAGAT TTCTGGAGAA TGCGTGGCCA GAGGTCTGGG GTGAAGAAGA 
     3121 ACCTGAGGAA AAGTCGGGAA GACCTCACAG CTGTTGTGTC TGTCAGCACC AAGTTCCCAG 
     3181 CTTATGAGAG GGTTTTGCTG CGAGAAGCTG GTTTCAAGAG ACCTGTGGTC TTATTCGGCC 
     3241 CCATAGCTGA TATAGCAATG GAAAAATTGG CTAATGAGTT ACCTGACTGG TTTCAAACTG 
     3301 CTAAAACGGA ACCAAAAGAT GCAGGATCTG AGAAATCCAC TGGAGTGGTC CGGTTAAATA 
     3361 CCGTGAGGCA AATTATTGAA CAGGATAAGC ATGCACTACT GGATGTGACT CCGAAAGCTG 
     3421 TGGACCTGTT GAATTACACC CAGTGGTTCC CAATTGTGAT TTTTTTCAAC CCAGACTCCA 
     3481 GACAAGGTGT CAAAACCATG AGACAAAGGT TAAATCCAAC GTCCAACAAA AGTTCTCGAA 
     3541 AGTTATTTGA TCAAGCCAAC AAGCTTAAAA AAACGTGTGC ACACCTTTTT ACAGCTACAA 
     3601 TCAACCTAAA TTCAGCCAAT GATAGCTGGT TTGGCAGCTT AAAGGACACT ATTCAGCATC 
     3661 AGCAAGGAGA AGCGGTTTGG GTCTCTGAAG GAAAGATGGA AGGGATGGAT GATGACCCCG 
     3721 AAGACCGCAT GTCCTACTTA ACCGCCATGG GCGCGGACTA TCTGAGTTGC GACAGCCGCC 
     3781 TCATCAGTGA CTTTGAAGAC ACGGACGGTG AAGGAGGCGC CTACACTGAC AATGAGCTGG 
     3841 ATGAGCCAGC CGAGGAGCCG CTGGTGTCGT CCATCACCCG CTCCTCGGAG CCGGTGCAGC 
     3901 ACGAGGAGAT CGAAATTGCC CAGAAGCATC CTGATATCTA TGCAGTTCCA ATCAAAACGC 
     3961 ACAAGCCAGA CCCTGGCACG CCCCAGCACA CGAGTTCCAG ACCCCCTGAG CCACAGAAAG 
     4021 CTCCTTCCAG ACCTTATCAG GATACCAGAG GAAGTTATGG CAGTGATGCC GAGGAGGAGG 
     4081 AGTACCGCCA GCAGCTGTCA GAACACTCCA AGCGCGGTTA CTATGGCCAG TCTGCCCGAT 
     4141 ACCGGGACAC AGAATTAACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4201 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4261 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4321 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4381 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4441 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4501 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4561 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4621 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4681 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4741 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     4801 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     4861 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     4921 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     4981 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5041 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5101 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5161 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5221 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5281 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5341 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5401 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5461 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5521 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5581 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     5641 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     5701 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     5761 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     5821 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     5881 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     5941 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6001 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6061 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6121 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6181 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6241 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6301 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6361 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6421 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6481 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6541 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6601 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     6661 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     6721 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     6781 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     6841 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     6901 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     6961 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7021 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7081 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7141 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7201 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7261 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7321 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7381 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7441 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7501 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7561 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7621 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     7681 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     7741 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     7801 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     7861 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     7921 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     7981 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//