LOCUS       RC223152                8823 bp    DNA     circular  SYN 
DEFINITION  CADPS (Myc-DDK-tagged)-Human Ca++-dependent secretion activator (CADPS), transcript variant 2
ACCESSION   NM_183394
VERSION     NM_183394.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4980..5071 
                     /label="Myc-DDK"  
     misc_feature    5101..5686 
                     /label="PolyA signal"  
     misc_feature    5158..5177 
                     /label="Restriction Enzyme"  
     misc_feature    5962..6634 
                     /label="ColE1"  
     misc_feature    7064..7858 
                     /label="Kan/Neo"  
     misc_feature    7893..8195 
                     /label="SV40 ori"  
     misc_feature    8281..8758 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4962..4968 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4962 
                     /label="CADPS"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTGGACCCT TCGTCCAGCG AAGAAGAATC GGATGAGATC GTGGAGGAGG 
     1081 AGAGCGGCAA GGAGGTGCTC GGCTCGGCCC CGTCCGGCGC GCGCCTGTCT CCCAGCCGTA 
     1141 CCAGCGAGGG CTCGGCCGGC AGCGCCGGGC TGGGGGGCGG CGGCGCCGGC GCCGGAGCCG 
     1201 GGGTGGGTGC AGGCGGCGGC GGGGGCAGCG GCGCGAGCAG CGGCGGCGGG GCCGGGGGGC 
     1261 TGCAACCCAG CAGCCGCGCT GGCGGCGGCC GGCCCTCCAG CCCCAGCCCG TCGGTGGTGA 
     1321 GCGAGAAGGA GAAGGAAGAG TTGGAGCGGC TGCAGAAAGA GGAGGAGGAG AGGAAGAAGA 
     1381 GGCTGCAGCT GTATGTGTTC GTGATGCGCT GCATCGCCTA CCCCTTTAAT GCCAAGCAGC 
     1441 CCACCGACAT GGCTCGCCGG CAGCAGAAGA TCAGCAAACA GCAGCTGCAG ACAGTCAAGG 
     1501 ACCGGTTTCA GGCTTTCCTC AATGGGGAAA CCCAGATCAT GGCTGACGAA GCCTTCATGA 
     1561 ACGCTGTGCA GAGTTACTAT GAGGTGTTCC TGAAGAGCGA CCGTGTGGCC CGCATGGTTC 
     1621 AGAGTGGAGG CTGTTCCGCC AACGACTCCC GGGAGGTCTT CAAGAAGCAC ATTGAGAAGA 
     1681 GAGTGCGCAG CCTGCCTGAG ATTGACGGCC TCAGCAAGGA GACTGTGCTG AGCTCCTGGA 
     1741 TGGCCAAATT TGATGCCATC TACCGTGGAG AAGAGGACCC GCGGAAGCAG CAGGCCCGGA 
     1801 TGACAGCCAG CGCAGCCTCC GAGCTGATTC TGAGCAAGGA GCAACTCTAT GAGATGTTCC 
     1861 AGAACATTCT TGGGATCAAG AAGTTCGAAC ATCAGCTCCT TTACAATGCC TGCCAGCTGG 
     1921 ACAATCCAGA TGAGCAAGCA GCCCAGATCA GACGAGAGCT GGATGGACGT CTACAAATGG 
     1981 CAGACCAAAT AGCCAGGGAA CGCAAATTTC CCAAGTTTGT ATCCAAAGAA ATGGAAAACA 
     2041 TGTACATTGA GGAGCTGAAG TCATCTGTCA ACCTGCTCAT GGCCAACTTG GAGAGCATGC 
     2101 CGGTATCCAA AGGCGGGGAG TTCAAGCTCC AGAAACTCAA ACGCAGCCAC AATGCTTCCA 
     2161 TCATCGACAT GGGCGAGGAG AGTGAGAACC AGCTCTCCAA GTCAGATGTC GTGCTGTCTT 
     2221 TCTCATTGGA GGTGGTAATT ATGGAAGTCC AAGGCCTCAA ATCTTTGGCT CCAAATCGCA 
     2281 TCGTATATTG CACAATGGAG GTGGAAGGAG GAGAGAAACT ACAGACTGAT CAGGCCGAGG 
     2341 CTTCTAAACC AACCTGGGGC ACCCAGGGTG ACTTCTCCAC AACCCATGCA CTGCCAGCTG 
     2401 TGAAGGTGAA GCTGTTCACA GAGAGCACAG GCGTCCTGGC GTTGGAGGAC AAGGAGCTTG 
     2461 GGCGGGTTAT TCTCCATCCC ACCCCGAACA GCCCCAAACA GTCAGAGTGG CACAAAATGA 
     2521 CAGTCTCCAA AAACTGCCCC GACCAAGATC TCAAAATCAA ACTTGCTGTC CGAATGGATA 
     2581 AGCCTCAAAA CATGAAGCAT TCTGGGTATT TATGGGCCAT CGGTAAGAAT GTCTGGAAGA 
     2641 GATGGAAGAA AAGGTTTTTT GTATTGGTGC AGGTCAGTCA GTACACGTTT GCCATGTGCA 
     2701 GTTATCGGGA GAAGAAAGCG GAGCCTCAGG AACTTCTACA ATTGGATGGC TACACTGTGG 
     2761 ATTACACCGA CCCCCAGCCA GGTTTGGAGG GTGGCCGAGC CTTCTTCAAT GCTGTCAAGG 
     2821 AGGGAGACAC CGTGATATTT GCCAGTGACG ATGAACAAGA CCGCATCCTG TGGGTCCAGG 
     2881 CCATGTATCG GGCCACGGGG CAGTCACACA AGCCTGTGCC CCCGACCCAA GTCCAGAAAC 
     2941 TCAACGCCAA GGGAGGAAAT GTACCTCAGC TGGATGCCCC TATCTCTCAA TTTTACGCAG 
     3001 ATAGAGCTCA AAAACATGGC ATGGATGAAT TTATCTCTTC CAACCCCTGT AACTTTGACC 
     3061 ACGCTTCCCT CTTTGAGATG GTACAACGCC TTACTTTGGA TCACAGACTT AATGATTCCT 
     3121 ATTCTTGCCT GGGCTGGTTC AGTCCTGGCC AGGTGTTTGT ACTAGACGAG TATTGCGCCC 
     3181 GAAATGGAGT CCGGGGGTGT CACCGACATC TCTGCTACCT CAGAGACTTG CTTGAACGGG 
     3241 CAGAAAATGG CGCCATGATC GACCCCACCC TTCTTCACTA CAGCTTTGCC TTCTGTGCAT 
     3301 CCCATGTCCA TGGGAACAGG CCTGATGGAA TTGGAACTGT GACTGTTGAA GAAAAGGAAC 
     3361 GTTTTGAAGA AATCAAAGAG AGGCTCCGAG TTCTGCTAGA AAATCAGATT ACACATTTTA 
     3421 GGTATTGCTT TCCATTTGGT CGACCTGAAG GTGCTTTGAA AGCTACTCTC TCACTCTTGG 
     3481 AAAGGGTTTT GATGAAAGAT ATTGTTACCC CAGTGCCACA AGAGGAGGTA AAAACAGTTA 
     3541 TCCGTAAATG TCTGGAACAG GCTGCGTTAG TCAACTATTC TCGGCTCTCA GAGTATGCCA 
     3601 AAATCGAAGG GAAAAAGAGA GAAATGTATG AGCATCCTGT CTTCTGCTTG GCCTCCCAAG 
     3661 TGATGGATTT AACCATTCAA AATGTAGGCC GGTTAATCAC TCCTGCCAAA AAGCTTGAAG 
     3721 ATACAATACG TCTTGCTGAA CTAGTCATTG AAGTTCTTCA GCAAAATGAG GAGCACCACG 
     3781 CAGAGGCCTT TGCGTGGTGG TCAGATTTAA TGGTGGAGCA TGCGGAGACG TTCCTGTCAC 
     3841 TCTTTGCAGT AGACATGGAT GCAGCCTTAG AGGTGCAACC TCCAGACACA TGGGACAGTT 
     3901 TTCCACTATT TCAGCTGCTG AATGATTTTC TCCGTACTGA CTATAATTTG TGCAATGGAA 
     3961 AATTTCACAA ACACCTGCAA GACCTGTTTG CCCCACTTGT TGTTAGATAT GTGGATCTGA 
     4021 TGGAGTCCTC AATTGCACAA TCCATTCACA GGGGCTTTGA GCGGGAGTCA TGGGAACCAG 
     4081 TCAATAATGG GTCAGGCACC TCAGAAGATC TGTTTTGGAA ACTTGACGCC CTTCAGACCT 
     4141 TCATTCGGGA CCTGCACTGG CCTGAAGAAG AGTTTGGAAA GCACCTGGAA CAACGGCTGA 
     4201 AGTTGATGGC AAGTGACATG ATCGAATCTT GTGTCAAAAG AACCAGGATT GCATTTGAAG 
     4261 TTAAGCTGCA AAAAACCAGT CGATCAACAG ATTTTCGAGT CCCACAGTCA ATATGCACCA 
     4321 TGTTTAATGT TATGGTTGAT GCCAAAGCTC AATCAACAAA ACTTTGCAGC ATGGAAATGG 
     4381 GCCAAGAGCA TCAATACCAT TCAAAAATAG ACGAACTAAT TGAAGAAACT GTTAAAGAAA 
     4441 TGATAACACT CTTGGTTGCA AAGTTCGTTA CTATCTTGGA AGGAGTGCTG GCAAAATTAT 
     4501 CCAGATATGA CGAAGGGACT TTGTTTTCTT CTTTTCTGTC ATTTACCGTG AAGGCAGCTT 
     4561 CCAAATATGT GGATGTACCT AAACCCGGGA TGGACGTGGC CGACGCCTAC GTGACTTTCG 
     4621 TCCGCCATTC TCAGGATGTC CTGCGTGATA AGGTCAATGA GGAGATGTAC ATAGAAAGGT 
     4681 TATTTGATCA ATGGTACAAC AGCTCCATGA ACGTGATCTG CACCTGGTTG ACGGACCGGA 
     4741 TGGACTTACA GCTTCATATT TATCAGTTGA AAACACTAAT TAGGATGGTA AAGAAAACCT 
     4801 ACAGAGATTT CCGATTGCAA GGGGTCCTGG ACTCCACCTT AAACAGCAAG ACCTATGAAA 
     4861 CGATCCGGAA CCGTCTCACT GTGGAGGAAG CCACAGCATC AGTGAGTGAA GGTGGGGGAC 
     4921 TGCAGGGCAT CAGCATGAAG GACAGCGATG AGGAAGACGA AGAAGACGAT ACGCGTACGC 
     4981 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     5041 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     5101 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     5161 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     5221 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     5281 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5341 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5401 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5461 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5521 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5581 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5641 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5701 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5761 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5821 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5881 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5941 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     6001 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     6061 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     6121 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     6181 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     6241 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     6301 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6361 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6421 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6481 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6541 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6601 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6661 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6721 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6781 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6841 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6901 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6961 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     7021 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     7081 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     7141 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     7201 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     7261 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     7321 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7381 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7441 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7501 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7561 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7621 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7681 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7741 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7801 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7861 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7921 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7981 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     8041 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     8101 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     8161 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     8221 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     8281 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     8341 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8401 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8461 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8521 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8581 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8641 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8701 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8761 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8821 TTT
//