LOCUS       RC223141                9084 bp    DNA     circular  SYN 
DEFINITION  DISP2 (Myc-DDK-tagged)-Human dispatched homolog 2 (Drosophila) (DISP2)
ACCESSION   NM_033510
VERSION     NM_033510.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5241..5332 
                     /label="Myc-DDK"  
     misc_feature    5362..5947 
                     /label="PolyA signal"  
     misc_feature    5419..5438 
                     /label="Restriction Enzyme"  
     misc_feature    6223..6895 
                     /label="ColE1"  
     misc_feature    7325..8119 
                     /label="Kan/Neo"  
     misc_feature    8154..8456 
                     /label="SV40 ori"  
     misc_feature    8542..9019 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5223..5229 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5223 
                     /label="DISP2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACGGTGAC AGCAGCAGCA GCAGCGGCGG CAGCGGTCCG GCTCCCGGCC 
     1081 CGGGTCCGGA AGGGGAGCAA CGGCCCGAGG GGGAGCCCTT GGCCCCAGAC GGCGGCTCCC 
     1141 CGGACAGCAC CCAGACCAAG GCTGTGCCCC CTGAGGCAAG CCCAGAGAGA AGCTGCTCCC 
     1201 TCCACAGCTG CCCCCTGGAG GACCCTTCCA GCTCTTCAGG ACCCCCACCA ACAACTTCCA 
     1261 CCCTCCAGCC TGTGGGTCCA TCCAGCCCCT TGGCCCCTGC CCACTTCACC TATCCCCGGG 
     1321 CACTGCAGGA ATACCAGGGG GGCAGTTCCC TGCCAGGACT TGGGGATCGG GCAGCTCTCT 
     1381 GCTCCCACGG CTCCAGCCTC AGCCCTTCTC CAGCCCCCTC ACAGCGCGAT GGGACCTGGA 
     1441 AGCCACCCGC TGTGCAGCAC CATGTGGTCA GCGTCAGGCA GGAACGAGCC TTCCAGATGC 
     1501 CAAAGAGCTA TTCCCAGCTG ATTGCTGAGT GGCCAGTGGC CGTGCTGATG CTGTGTCTGG 
     1561 CTGTCATCTT CCTCTGCACC CTGGCTGGAC TGTTGGGGGC CCGGCTGCCC GACTTCTCCA 
     1621 AGCCTTTGCT GGGCTTTGAG CCACGGGACA CAGACATTGG GAGCAAGTTA GTGGTCTGGA 
     1681 GAGCACTACA AGCCCTCACA GGCCCCAGGA AGCTGCTTTT CCTTTCCCCA GACCTTGAGC 
     1741 TGAACAGCTC GAGCTCCCAC AACACTCTGA GGCCTGCACC CAGAGGCAGT GCCCAGGAGA 
     1801 GCGCTGTCCG GCCTCGGAGA ATGGTGGAGC CCCTGGAGGA CAGAAGGCAA GAGAACTTCT 
     1861 TCTGTGGCCC CCCTGAGAAG AGCTATGCAA AGCTGGTGTT CATGTCCACC TCCTCGGGCA 
     1921 GCCTATGGAA CCTGCATGCC ATCCATTCCA TGTGTCGCAT GGAACAGGAC CAGATCCGCT 
     1981 CCCATACCAG CTTCGGGGCT CTGTGCCAGC GGACAGCAGC CAACCAGTGC TGCCCCAGCT 
     2041 GGTCCCTGGG CAACTATCTG GCTGTGCTCT CCAACCGCTC CTCCTGCCTG GACACTACCC 
     2101 AAGCTGACGC AGCCCGCACA CTGGCCCTGC TTCGGACCTG TGCCCTCTAC TACCACAGTG 
     2161 GCGCCTTGGT GCCCTCTTGT CTGGGACCTG GGCAGAACAA GTCCCCACGC TGTGCCCAGG 
     2221 TTCCCACCAA GTGCTCCCAG AGTAGTGCCA TCTACCAACT CCTGCACTTT CTGCTTGACA 
     2281 GGGACTTTCT GAGTCCCCAG ACCACTGACT ACCAGGTGCC TTCCCTCAAG TACAGCCTGC 
     2341 TCTTCCTGCC CACCCCAAAG GGTGCTTCCC TCATGGACAT CTACCTGGAC CGGCTGGCCA 
     2401 CCCCCTGGGG GCTTGCTGAC AACTACACCT CTGTCACTGG CATGGACCTG GGCCTCAAGC 
     2461 AGGAGCTGCT GAGGCACTTC CTGGTCCAGG ACACGGTGTA CCCCTTGCTG GCTCTGGTTG 
     2521 CCATCTTCTT CGGCATGGCC CTGTACCTGC GCTCACTCTT CCTCACGCTC ATGGTGCTGC 
     2581 TGGGGGTGCT GGGCTCACTG CTGGTGGCCT TCTTCCTTTA CCAGGTGGCC TTCCGCATGG 
     2641 CCTACTTCCC CTTCGTCAAT CTGGCAGCCC TCCTCCTGCT GAGCAGCGTC TGCGCCAACC 
     2701 ACACGCTCAT CTTCTTCGAC CTGTGGCGCC TTAGCAAGAG CCAGCTGCCG TCGGGGGGGC 
     2761 TGGCGCAGCG CGTGGGCCGC ACCATGCACC ACTTCGGCTA CCTGCTGCTG GTCTCCGGCC 
     2821 TCACCACGAG CGCGGCCTTC TATGCCAGCT ACCTGAGCCG CCTGCCGGCC GTTCGCTGCC 
     2881 TCGCCCTCTT CATGGGCACG GCTGTGCTGG TGCACCTGGC GCTCACGCTG GTCTGGCTGC 
     2941 CCGCCTCCGC CGTGCTCCAC GAGCGCTACC TGGCGCGCGG CTGTGCGCGC CGGGCGCGGG 
     3001 GCCGGTGGGA GGGCAGCGCG CCCCGGCGGC TACTGCTGGC GCTGCACCGG CGGCTCCGCG 
     3061 GCCTGCGGAG GGCGGCGGCT GGCACCTCGC GTCTGCTCTT CCAGCGCCTG CTGCCCTGCG 
     3121 GCGTCATCAA GTTCCGCTAC ATCTGGATCT GCTGGTTCGC AGCACTGGCG GCAGGGGGCG 
     3181 CCTACATCGC CGGAGTCAGC CCCCGCCTGC GGCTGCCCAC GCTGCCGCCG CCCGGCGGCC 
     3241 AGGTCTTCCG GCCCAGCCAC CCCTTCGAGC GCTTCGACGC GGAGTATCGC CAGCTGTTCC 
     3301 TGTTCGAGCA GCTGCCGCAG GGCGAGGGCG GCCACATGCC CGTGGTTTTG GTGTGGGGCG 
     3361 TCCTGCCTGT GGACACTGGC GACCCTCTGG ACCCTCGTAG CAACAGCAGC CTGGTGAGGG 
     3421 ACCCTGCCTT CTCGGCCAGC GGCCCTGAGG CCCAGCGCTG GCTGCTGGCA CTCTGTCACC 
     3481 GGGCCCGGAA TCAGAGCTTC TTCGACACCC TGCAGGAAGG CTGGCCCACG CTGTGTTTCG 
     3541 TGGAGACCCT CCAGCGCTGG ATGGAGAGCC CCAGCTGCGC CCGCCTGGGG CCTGACCTCT 
     3601 GCTGCGGCCA CTCGGACTTC CCCTGGGCCC CCCAGTTTTT CCTGCACTGC CTGAAAATGA 
     3661 TGGCTCTGGA GCAAGGCCCC GATGGCACCC AGGACCTGGG ACTCCGCTTT GATGCCCATG 
     3721 GCAGCCTGGC CGCCCTGGTC CTACAATTCC AGACCAACTT CCGGAACAGT CCGGACTACA 
     3781 ACCAGACCCA GCTCTTCTAC AATGAGGTCA GCCACTGGCT GGCAGCGGAG CTGGGCATGG 
     3841 CACCTCCAGG CCTCCGCCGT GGTTGGTTCA CTAGCCGTCT AGAGCTGTAT AGCCTGCAGC 
     3901 ACAGCCTGAG CACTGAGCCT GCTGTGGTGC TGGGCCTGGC TTTGGCGCTG GCCTTTGCCA 
     3961 CACTGCTCCT GGGCACCTGG AATGTTCCCC TCAGCCTATT CTCCGTGGCA GCTGTGGCAG 
     4021 GCACCGTGCT GCTCACTGTA GGACTCCTGG TTCTCCTCGA GTGGCAGCTC AACACTGCCG 
     4081 AGGCCCTGTT TCTCTCTGCC TCAGTGGGCC TCTCAGTAGA CTTCACTGTC AACTACTGCA 
     4141 TCTCCTATCA CCTGTGCCCA CACCCTGACC GCCTGAGCCG TGTGGCCTTC TCTCTGCGCC 
     4201 AGACCAGCTG CGCCACAGCC GTGGGGGCTG CAGCCCTGTT TGCGGCAGGC GTGCTCATGC 
     4261 TGCCTGCCAC AGTGCTGCTC TATCGCAAGC TGGGCATCAT CCTCATGATG GTCAAATGCG 
     4321 TCAGTTGTGG CTTTGCCAGC TTCTTCTTCC AATCTCTCTG CTGTTTCTTC GGGCCAGAGA 
     4381 AGAACTGTGG GCAGATCCTC TGGCCCTGTG CCCACCTGCC ATGGGATGCT GGTACTGGGG 
     4441 ACCCTGGTGG GGAGAAGGCA GGCCGCCCAC GACCAGGGTC AGTGGGAGGG ATGCCCGGGT 
     4501 CCTGCTCAGA GCAATATGAG CTACAGCCCC TGGCACGGCG TCGGAGCCCC AGCTTTGACA 
     4561 CCAGCACAGC CACCAGCAAG CTGTCCCACC GGCCCTCAGT ACTCTCTGAG GATCTGCAGC 
     4621 TCCATGATGG TCCGTGCTGT TCCCGGCCCC CACCAGCCCC TGCCTCCCCA AGGGAGCTGC 
     4681 TGCTGGACCA CCAGGCAGTC TTCAGCCAGT GCCCTGCCCT GCAGACCTCC TCCCCCTATA 
     4741 AGCAGGCTGG CCCCAGCCCC AAAACCCGGG CCAGGCAGGA CTCCCAAGGG GAGGAGGCTG 
     4801 AGCCCCTGCC AGCCTCACCA GAAGCCCCAG CCCACTCTCC TAAGGCCAAG GCTGCAGATC 
     4861 CTCCTGATGG CTTCTGTTCC TCAGCCAGCA CCCTGGAGGG GCTCAGCGTC TCTGATGAGA 
     4921 CCTGCCTAAG CACCTCTGAG CCCAGTGCCC GTGTACCAGA TTCCGTGGGT GTGTCCCCAG 
     4981 ATGACCTGGA TGACACTGGG CAGCCAGTCC TTGAGCGAGG CCAGCTCAAT GGGAAGCGGG 
     5041 ACACCCTGTG GCTGGCGCTG AGGGAGACAG TGTATGACCC ATCATTGCCC GCTTCCCATC 
     5101 ACAGCAGCTT GTCCTGGAAG GGCCGAGGGG GGCCAGGGGA TGGCAGCCCT GTGGTGCTGC 
     5161 CCAATAGCCA GCCAGACCTG CCAGATGTTT GGCTGCGCAG GCCCAGCACT CACACGTCAG 
     5221 GCTATAGCAG CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     5281 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     5341 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     5401 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     5461 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     5521 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5581 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5641 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5701 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5761 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5821 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5881 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5941 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     6001 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     6061 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     6121 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     6181 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     6241 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     6301 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     6361 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     6421 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     6481 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6541 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6601 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6661 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6721 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6781 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6841 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6901 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6961 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     7021 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     7081 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     7141 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     7201 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     7261 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     7321 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     7381 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     7441 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     7501 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7561 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7621 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7681 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7741 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7801 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7861 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7921 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7981 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     8041 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     8101 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     8161 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     8221 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     8281 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     8341 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8401 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8461 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     8521 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8581 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8641 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8701 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8761 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8821 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8881 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8941 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     9001 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     9061 TAACAAAAAT TTAACGCGAA TTTT
//