LOCUS       RC223136                9267 bp    DNA     circular  SYN 
DEFINITION  GRIN2A (Myc-DDK-tagged)-Human glutamate receptor, ionotropic, N-methyl D-aspartate 2A (GRIN2A), transcript variant 2
ACCESSION   NM_000833
VERSION     NM_000833.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5422..5513 
                     /label="Myc-DDK"  
     misc_feature    5543..6128 
                     /label="PolyA signal"  
     misc_feature    5600..5619 
                     /label="Restriction Enzyme"  
     misc_feature    6404..7076 
                     /label="ColE1"  
     misc_feature    7506..8300 
                     /label="Kan/Neo"  
     misc_feature    8335..8637 
                     /label="SV40 ori"  
     misc_feature    8723..9200 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5412..5420 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5412 
                     /label="GRIN2A"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCAGAGTG GGCTATTGGA CCCTGCTGGT GCTGCCGGCC CTTCTGGTCT 
     1081 GGCGCGGTCC GGCGCCGAGC GCGGCGGCGG AGAAGGGTCC CCCCGCGCTA AATATTGCGG 
     1141 TGATGCTGGG TCACAGCCAC GACGTGACAG AGCGCGAACT TCGAACACTG TGGGGCCCCG 
     1201 AGCAGGCGGC GGGGCTGCCC CTGGACGTGA ACGTGGTAGC TCTGCTGATG AACCGCACCG 
     1261 ACCCCAAGAG CCTCATCACG CACGTGTGCG ACCTCATGTC CGGGGCACGC ATCCACGGCC 
     1321 TCGTGTTTGG GGACGACACG GACCAGGAGG CCGTAGCCCA GATGCTGGAT TTTATCTCCT 
     1381 CCCACACCTT CGTCCCCATC TTGGGCATTC ATGGGGGCGC ATCTATGATC ATGGCTGACA 
     1441 AGGATCCGAC GTCTACCTTC TTCCAGTTTG GAGCGTCCAT CCAGCAGCAA GCCACGGTCA 
     1501 TGCTGAAGAT CATGCAGGAT TATGACTGGC ATGTCTTCTC CCTGGTGACC ACTATCTTCC 
     1561 CTGGCTACAG GGAATTCATC AGCTTCGTCA AGACCACAGT GGACAACAGC TTTGTGGGCT 
     1621 GGGACATGCA GAATGTGATC ACACTGGACA CTTCCTTTGA GGATGCAAAG ACACAAGTCC 
     1681 AGCTGAAGAA GATCCACTCT TCTGTCATCT TGCTCTACTG TTCCAAAGAC GAGGCTGTTC 
     1741 TCATTCTGAG TGAGGCCCGC TCCCTTGGCC TCACCGGGTA TGATTTCTTC TGGATTGTCC 
     1801 CCAGCTTGGT CTCTGGGAAC ACGGAGCTCA TCCCAAAAGA GTTTCCATCG GGACTCATTT 
     1861 CTGTCTCCTA CGATGACTGG GACTACAGCC TGGAGGCGAG AGTGAGGGAC GGCATTGGCA 
     1921 TCCTAACCAC CGCTGCATCT TCTATGCTGG AGAAGTTCTC CTACATCCCC GAGGCCAAGG 
     1981 CCAGCTGCTA CGGGCAGATG GAGAGGCCAG AGGTCCCGAT GCACACCTTG CACCCATTTA 
     2041 TGGTCAATGT TACATGGGAT GGCAAAGACT TATCCTTCAC TGAGGAAGGC TACCAGGTGC 
     2101 ACCCCAGGCT GGTGGTGATT GTGCTGAACA AAGACCGGGA ATGGGAAAAG GTGGGCAAGT 
     2161 GGGAGAACCA TACGCTGAGC CTGAGGCACG CCGTGTGGCC CAGGTACAAG TCCTTCTCCG 
     2221 ACTGTGAGCC GGATGACAAC CATCTCAGCA TCGTCACCCT GGAGGAGGCC CCATTCGTCA 
     2281 TCGTGGAAGA CATAGACCCC CTGACCGAGA CGTGTGTGAG GAACACCGTG CCATGTCGGA 
     2341 AGTTCGTCAA AATCAACAAT TCAACCAATG AGGGGATGAA TGTGAAGAAA TGCTGCAAGG 
     2401 GGTTCTGCAT TGATATTCTG AAGAAGCTTT CCAGAACTGT GAAGTTTACT TACGACCTCT 
     2461 ATCTGGTGAC CAATGGGAAG CATGGCAAGA AAGTTAACAA TGTGTGGAAT GGAATGATCG 
     2521 GTGAAGTGGT CTATCAACGG GCAGTCATGG CAGTTGGCTC GCTCACCATC AATGAGGAAC 
     2581 GTTCTGAAGT GGTGGACTTC TCTGTGCCCT TTGTGGAAAC GGGAATCAGT GTCATGGTTT 
     2641 CAAGAAGTAA TGGCACCGTC TCACCTTCTG CTTTTCTAGA ACCATTCAGC GCCTCTGTCT 
     2701 GGGTGATGAT GTTTGTGATG CTGCTCATTG TTTCTGCCAT AGCTGTTTTT GTCTTTGAAT 
     2761 ACTTCAGCCC TGTTGGATAC AACAGAAACT TAGCCAAAGG GAAAGCACCC CATGGGCCTT 
     2821 CTTTTACAAT TGGAAAAGCT ATATGGCTTC TTTGGGGCCT GGTGTTCAAT AACTCCGTGC 
     2881 CTGTCCAGAA TCCTAAAGGG ACCACCAGCA AGATCATGGT ATCTGTATGG GCCTTCTTCG 
     2941 CTGTCATATT CCTGGCTAGC TACACAGCCA ATCTGGCTGC CTTCATGATC CAAGAGGAAT 
     3001 TTGTGGACCA AGTGACCGGC CTCAGTGACA AAAAGTTTCA GAGACCTCAT GACTATTCCC 
     3061 CACCTTTTCG ATTTGGGACA GTGCCTAATG GAAGCACGGA GAGAAACATT CGGAATAACT 
     3121 ATCCCTACAT GCATCAGTAC ATGACCAAAT TTAATCAGAA AGGAGTAGAG GACGCCTTGG 
     3181 TCAGCCTGAA AACGGGGAAG CTGGACGCTT TCATCTACGA TGCCGCAGTC TTGAATTACA 
     3241 AGGCTGGGAG GGATGAAGGC TGCAAGCTGG TGACCATCGG GAGTGGGTAC ATCTTTGCCA 
     3301 CCACCGGTTA TGGAATTGCC CTTCAGAAAG GCTCTCCTTG GAAGAGGCAG ATCGACCTGG 
     3361 CCTTGCTTCA GTTTGTGGGT GATGGTGAGA TGGAGGAGCT GGAGACCCTG TGGCTCACTG 
     3421 GGATCTGCCA CAACGAGAAG AACGAGGTGA TGAGCAGCCA GCTGGACATT GACAACATGG 
     3481 CGGGCGTATT CTACATGCTG GCTGCCGCCA TGGCCCTTAG CCTCATCACC TTCATCTGGG 
     3541 AGCACCTCTT CTACTGGAAG CTGCGCTTCT GTTTCACGGG CGTGTGCTCC GACCGGCCTG 
     3601 GGTTGCTCTT CTCCATCAGC AGGGGCATCT ACAGCTGCAT TCATGGAGTG CACATTGAAG 
     3661 AAAAGAAGAA GTCTCCAGAC TTCAATCTGA CGGGATCCCA GAGCAACATG TTAAAACTCC 
     3721 TCCGGTCAGC CAAAAACATT TCCAGCATGT CCAACATGAA CTCCTCAAGA ATGGACTCAC 
     3781 CCAAAAGAGC TGCTGACTTC ATCCAAAGAG GTTCCCTCAT CATGGACATG GTTTCAGATA 
     3841 AGGGGAATTT GATGTACTCA GACAACAGGT CCTTTCAGGG GAAAGAGAGC ATTTTTGGAG 
     3901 ACAACATGAA CGAACTCCAA ACATTTGTGG CCAACCGGCA GAAGGATAAC CTCAATAACT 
     3961 ATGTATTCCA GGGACAACAT CCTCTTACTC TCAATGAGTC CAACCCTAAC ACGGTGGAGG 
     4021 TGGCCGTGAG CACAGAATCC AAAGCGAACT CTAGACCCCG GCAGCTGTGG AAGAAATCCG 
     4081 TGGATTCCAT ACGCCAGGAT TCACTATCCC AGAATCCAGT CTCCCAGAGG GATGAGGCAA 
     4141 CAGCAGAGAA TAGGACCCAC TCCCTAAAGA GCCCTAGGTA TCTTCCAGAA GAGATGGCCC 
     4201 ACTCTGACAT TTCAGAAACG TCAAATCGGG CCACGTGCCA CAGGGAACCT GACAACAGTA 
     4261 AGAACCACAA AACCAAGGAC AACTTTAAAA GGTCAGTGGC CTCCAAATAC CCCAAGGACT 
     4321 GTAGTGAGGT CGAGCGCACC TACCTGAAAA CCAAATCAAG CTCCCCTAGA GACAAGATCT 
     4381 ACACTATAGA TGGTGAGAAG GAGCCTGGTT TCCACTTAGA TCCACCCCAG TTTGTTGAAA 
     4441 ATGTGACCCT GCCCGAGAAC GTGGACTTCC CGGACCCCTA CCAGGATCCC AGTGAAAACT 
     4501 TCCGCAAGGG GGACTCCACG CTGCCAATGA ACCGGAACCC CTTGCATAAT GAAGAGGGGC 
     4561 TTTCCAACAA CGACCAGTAT AAACTCTACT CCAAGCACTT CACCTTGAAA GACAAGGGTT 
     4621 CCCCGCACAG TGAGACCAGC GAGCGATACC GGCAGAACTC CACGCACTGC AGAAGCTGCC 
     4681 TTTCCAACAT GCCCACCTAT TCAGGCCACT TCACCATGAG GTCCCCCTTC AAGTGCGATG 
     4741 CCTGCCTGCG GATGGGGAAC CTCTATGACA TCGATGAAGA CCAGATGCTT CAGGAGACAG 
     4801 GTAACCCAGC CACCGGGGAG CAGGTCTACC AGCAGGACTG GGCACAGAAC AATGCCCTTC 
     4861 AATTACAAAA GAACAAGCTA AGGATTAGCC GTCAGCATTC CTACGATAAC ATTGTCGACA 
     4921 AACCTAGGGA GCTAGACCTT AGCAGGCCCT CCCGGAGCAT AAGCCTCAAG GACAGGGAAC 
     4981 GGCTTCTGGA GGGAAATTTT TACGGCAGCC TGTTTAGTGT CCCCTCAAGC AAACTCTCGG 
     5041 GGAAAAAAAG CTCCCTTTTC CCCCAAGGTC TGGAGGACAG CAAGAGGAGC AAGTCTCTCT 
     5101 TGCCAGACCA CACCTCCGAT AACCCTTTCC TCCACTCCCA CAGGGATGAC CAACGCTTGG 
     5161 TTATTGGGAG ATGCCCCTCG GACCCTTACA AACACTCGTT GCCATCCCAG GCGGTGAATG 
     5221 ACAGCTATCT TCGGTCGTCC TTGAGGTCAA CGGCATCGTA CTGTTCCAGG GACAGTCGGG 
     5281 GCCACAATGA TGTGTATATT TCGGAGCATG TTATGCCTTA TGCTGCAAAT AAGAATAATA 
     5341 TGTACTCTAC CCCCAGGGTT TTAAATTCCT GCAGCAATAG ACGCGTGTAC AAGAAAATGC 
     5401 CTAGTATCGA ATCTGATGTT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     5461 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     5521 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     5581 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     5641 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     5701 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     5761 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     5821 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5881 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5941 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     6001 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     6061 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     6121 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     6181 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     6241 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     6301 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     6361 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     6421 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     6481 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     6541 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     6601 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     6661 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     6721 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     6781 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6841 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6901 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6961 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     7021 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     7081 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     7141 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     7201 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     7261 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     7321 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     7381 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     7441 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     7501 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     7561 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     7621 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     7681 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     7741 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     7801 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7861 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7921 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7981 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     8041 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     8101 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     8161 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     8221 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     8281 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     8341 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     8401 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     8461 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     8521 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8581 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8641 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     8701 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     8761 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     8821 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8881 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8941 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     9001 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     9061 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     9121 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     9181 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     9241 ATTTAACAAA AATTTAACGC GAATTTT
//