LOCUS       RC223034                7824 bp    DNA     circular  SYN 
DEFINITION  MTHFD1L (Myc-DDK-tagged)-Human methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (MTHFD1L), nuclear gene encoding mitochondrial protein
ACCESSION   NM_015440
VERSION     NM_015440.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3979..4070 
                     /label="Myc-DDK"  
     misc_feature    4100..4685 
                     /label="PolyA signal"  
     misc_feature    4157..4176 
                     /label="Restriction Enzyme"  
     misc_feature    4961..5633 
                     /label="ColE1"  
     misc_feature    6063..6857 
                     /label="Kan/Neo"  
     misc_feature    6892..7194 
                     /label="SV40 ori"  
     misc_feature    7280..7757 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3954..3961 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3954 
                     /label="MTHFD1L"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCACGCGT CTGCCGCTCG TCCTGCGCCA GCTCCGCCGC CCGCCCCAGC 
     1081 CCCCGGGCCC TCCGCGCCGC CTCCGTGTGC CCTGTCGCGC TAGCAGCGGC GGCGGCGGAG 
     1141 GCGGCGGCGG TGGCCGGGAG GGCCTGCTTG GACAGCGGCG GCCGCAGGAT GGCCAGGCCC 
     1201 GGAGCAGCTG CAGCCCCGGC GGCCGAACGC CCGCGGCGCG GGACTCCATC GTCAGAGAAG 
     1261 TCATTCAGAA TTCAAAAGAA GTTCTAAGTT TATTGCAAGA AAAAAACCCT GCCTTCAAGC 
     1321 CGGTTCTTGC AATTATCCAG GCAGGTGACG ACAACTTGAT GCAGGAAATC AACCAGAATT 
     1381 TGGCTGAGGA GGCTGGTCTG AACATCACTC ACATTTGCCT CCCTCCAGAT AGCAGTGAAG 
     1441 CCGAGATTAT AGATGAAATC TTAAAGATCA ATGAAGATAC CAGAGTACAT GGCCTTGCCC 
     1501 TTCAGATCTC TGAGAACTTG TTTAGCAACA AAGTCCTCAA TGCCTTGAAA CCAGAAAAAG 
     1561 ATGTGGATGG AGTAACAGAC ATAAACCTGG GGAAGCTGGT GCGAGGGGAT GCCCATGAAT 
     1621 GTTTTGTTTC ACCTGTTGCC AAAGCTGTAA TTGAACTTCT TGAAAAATCA GGTGTCAACC 
     1681 TAGATGGAAA GAAGATTTTG GTAGTGGGGG CCCATGGGTC TTTGGAAGCT GCTCTACAAT 
     1741 GCCTGTTCCA GAGAAAAGGG TCCATGACAA TGAGCATCCA GTGGAAAACA CGCCAGCTTC 
     1801 AAAGCAAGCT TCACGAGGCT GACATTGTGG TCCTAGGCTC ACCTAAGCCA GAAGAGATTC 
     1861 CCCTTACTTG GATACAACCA GGAACTACTG TTCTCAACTG CTCCCATGAC TTCCTGTCAG 
     1921 GGAAGGTTGG GTGTGGCTCT CCAAGAATAC ATTTTGGTGG ACTCATTGAG GAAGATGATG 
     1981 TGATTCTCCT TGCTGCAGCT CTGCGAATTC AGAACATGGT CAGTAGTGGA AGGAGATGGC 
     2041 TTCGTGAACA GCAGCACAGG CGGTGGAGAC TTCACTGCTT GAAACTTCAG CCTCTCTCCC 
     2101 CTGTGCCAAG TGACATTGAG ATTTCAAGAG GACAAACTCC AAAAGCTGTG GATGTCCTTG 
     2161 CCAAGGAGAT TGGATTGCTT GCAGATGAAA TTGAAATCTA TGGCAAAAGC AAAGCCAAAG 
     2221 TACGTTTGTC CGTGCTAGAA AGGTTAAAGG ATCAAGCAGA TGGAAAATAC GTCTTAGTTG 
     2281 CTGGGATCAC ACCCACCCCT CTTGGAGAAG GGAAGAGCAC AGTCACCATC GGGCTTGTGC 
     2341 AGGCTCTGAC CGCACACCTG AATGTCAACT CCTTTGCCTG CTTGAGGCAG CCTTCCCAAG 
     2401 GACCGACGTT TGGAGTGAAA GGAGGAGCCG CGGGTGGTGG ATATGCCCAG GTCATCCCCA 
     2461 TGGAGGAGTT CAACCTTCAC TTGACTGGAG ACATCCACGC CATCACCGCT GCCAATAACT 
     2521 TGCTGGCTGC CGCCATCGAC ACGAGGATTC TTCATGAAAA CACGCAAACA GATAAGGCTC 
     2581 TGTATAATCG GCTGGTTCCT TTAGTGAATG GTGTCAGAGA ATTTTCAGAA ATTCAGCTTG 
     2641 CTCGGCTAAA AAAACTGGGA ATAAATAAGA CTGATCCGAG CACACTGACA GAAGAGGAAG 
     2701 TGAGTAAATT TGCCCGTCTC GACATCGACC CATCTACCAT CACGTGGCAG AGAGTATTGG 
     2761 ATACAAATGA CCGATTTCTA CGAAAAATAA CCATCGGGCA GGGAAACACA GAGAAGGGCC 
     2821 ATTACCGGCA GGCGCAGTTT GACATCGCAG TGGCCAGCGA GATCATGGCG GTGCTGGCCC 
     2881 TGACGGACAG CCTCGCAGAC ATGAAGGCAC GGCTGGGAAG GATGGTGGTG GCCAGTGACA 
     2941 AAAGCGGGCA GCCTGTGACA GCAGATGATT TGGGGGTGAC AGGTGCTTTG ACAGTTTTGA 
     3001 TGAAAGATGC AATAAAACCA AACCTGATGC AGACCCTGGA AGGGACACCT GTGTTCGTGC 
     3061 ATGCGGGCCC TTTTGCTAAC ATTGCTCACG GCAACTCTTC AGTGTTGGCT GATAAAATTG 
     3121 CCCTGAAACT GGTTGGTGAA GAAGGATTTG TAGTGACCGA AGCTGGCTTT GGTGCTGACA 
     3181 TCGGAATGGA GAAATTCTTC AACATCAAGT GCCGAGCTTC CGGCTTGGTG CCCAACGTGG 
     3241 TTGTGTTAGT GGCAACGGTG CGAGCTCTGA AGATGCATGG AGGCGGGCCA AGTGTAACGG 
     3301 CTGGTGTTCC TCTTAAGAAA GAATATACAG AGGAGAACAT CCAGCTGGTG GCAGACGGCT 
     3361 GCTGTAACCT CCAGAAGCAA ATTCAGATCA CTCAGCTCTT TGGGGTTCCC GTTGTGGTGG 
     3421 CTCTGAATGT CTTCAAGACC GACACCCGCG CTGAGATTGA CTTGGTGTGT GAGCTTGCAA 
     3481 AGCGGGCTGG TGCCTTTGAT GCAGTCCCCT GCTATCACTG GTCCGTTGGT GGAAAAGGAT 
     3541 CGGTGGACTT GGCTCGGGCT GTGAGAGAGG CTGCGAGTAA AAGAAGCCGA TTCCAGTTCC 
     3601 TGTATGATGT TCAGGTTCCA ATTGTGGACA AGATAAGGAC CATTGCTCAG GCTGTCTATG 
     3661 GAGCCAAAGA TATTGAACTC TCTCCTGAGG CACAAGCCAA AATAGATCGT TACACTCAAC 
     3721 AGGGTTTTGG AAATTTGCCC ATCTGCATGG CAAAGACCCA CCTTTCTCTA TCTCACCAAC 
     3781 CTGACAAAAA AGGTGTGCCA AGGGACTTCA TCTTACCTAT CAGTGACGTC CGGGCCAGCA 
     3841 TAGGCGCTGG GTTCATTTAC CCTTTGGTCG GAACGATGAG CACCATGCCA GGACTGCCCA 
     3901 CCCGGCCCTG CTTTTATGAC ATAGATCTTG ATACCGAAAC AGAACAAGTT AAAGGCTTGT 
     3961 TCAGCGGACC GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4021 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4081 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4141 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4201 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4261 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4321 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4381 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4441 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4501 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4561 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4621 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4681 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4741 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4801 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4861 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4921 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4981 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5041 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5101 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5161 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5221 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5281 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5341 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5401 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5461 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5521 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5581 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5641 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5701 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     5761 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5821 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5881 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5941 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6001 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6061 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6121 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6181 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6241 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6301 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6361 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6421 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6481 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6541 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6601 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6661 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6721 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6781 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6841 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6901 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6961 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7021 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7081 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7141 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7201 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7261 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7321 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7381 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7441 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7501 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7561 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7621 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7681 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7741 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7801 TAACAAAAAT TTAACGCGAA TTTT
//