LOCUS       RC223032                8874 bp    DNA     circular  SYN 
DEFINITION  ABCC4 (Myc-DDK-tagged)-Human ATP-binding cassette, sub-family C (CFTR/MRP), member 4 (ABCC4), transcript variant 1
ACCESSION   NM_005845
VERSION     NM_005845.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5029..5120 
                     /label="Myc-DDK"  
     misc_feature    5150..5735 
                     /label="PolyA signal"  
     misc_feature    5207..5226 
                     /label="Restriction Enzyme"  
     misc_feature    6011..6683 
                     /label="ColE1"  
     misc_feature    7113..7907 
                     /label="Kan/Neo"  
     misc_feature    7942..8244 
                     /label="SV40 ori"  
     misc_feature    8330..8807 
                     /label="f1 ori"  
     misc_feature    1029..1037 
                     /label="Restriction Enzyme"  
     misc_feature    5004..5011 
                     /label="Restriction Enzyme"  
     misc_feature    1029..5004 
                     /label="ABCC4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCGG CGCGCCCATG CTGCCCGTGT ACCAGGAGGT GAAGCCCAAC CCGCTGCAGG 
     1081 ACGCGAACAT CTGCTCACGC GTGTTCTTCT GGTGGCTCAA TCCCTTGTTT AAAATTGGCC 
     1141 ATAAACGGAG ATTAGAGGAA GATGATATGT ATTCAGTGCT GCCAGAAGAC CGCTCACAGC 
     1201 ACCTTGGAGA GGAGTTGCAA GGGTTCTGGG ATAAAGAAGT TTTAAGAGCT GAGAATGACG 
     1261 CACAGAAGCC TTCTTTAACA AGAGCAATCA TAAAGTGTTA CTGGAAATCT TATTTAGTTT 
     1321 TGGGAATTTT TACGTTAATT GAGGAAAGTG CCAAAGTAAT CCAGCCCATA TTTTTGGGAA 
     1381 AAATTATTAA TTATTTTGAA AATTATGATC CCATGGATTC TGTGGCTTTG AACACAGCGT 
     1441 ACGCCTATGC CACGGTGCTG ACTTTTTGCA CGCTCATTTT GGCTATACTG CATCACTTAT 
     1501 ATTTTTATCA CGTTCAGTGT GCTGGGATGA GGTTACGAGT AGCCATGTGC CATATGATTT 
     1561 ATCGGAAGGC ACTTCGTCTT AGTAACATGG CCATGGGGAA GACAACCACA GGCCAGATAG 
     1621 TCAATCTGCT GTCCAATGAT GTGAACAAGT TTGATCAGGT GACAGTGTTC TTACACTTCC 
     1681 TGTGGGCAGG ACCACTGCAG GCGATCGCAG TGACTGCCCT ACTCTGGATG GAGATAGGAA 
     1741 TATCGTGCCT TGCTGGGATG GCAGTTCTAA TCATTCTCCT GCCCTTGCAA AGCTGTTTTG 
     1801 GGAAGTTGTT CTCATCACTG AGGAGTAAAA CTGCAACTTT CACGGATGCC AGGATCAGGA 
     1861 CCATGAATGA AGTTATAACT GGTATAAGGA TAATAAAAAT GTACGCCTGG GAAAAGTCAT 
     1921 TTTCAAATCT TATTACCAAT TTGAGAAAGA AGGAGATTTC CAAGATTCTG AGAAGTTCCT 
     1981 GCCTCAGGGG GATGAATTTG GCTTCGTTTT TCAGTGCAAG CAAAATCATC GTGTTTGTGA 
     2041 CCTTCACCAC CTACGTGCTC CTCGGCAGTG TGATCACAGC CAGCCGCGTG TTCGTGGCAG 
     2101 TGACGCTGTA TGGGGCTGTG CGGCTGACGG TTACCCTCTT CTTCCCCTCA GCCATTGAGA 
     2161 GGGTGTCAGA GGCAATCGTC AGCATCCGAA GAATCCAGAC CTTTTTGCTA CTTGATGAGA 
     2221 TATCACAGCG CAACCGTCAG CTGCCGTCAG ATGGTAAAAA GATGGTGCAT GTGCAGGATT 
     2281 TTACTGCTTT TTGGGATAAG GCATCAGAGA CCCCAACTCT ACAAGGCCTT TCCTTTACTG 
     2341 TCAGACCTGG CGAATTGTTA GCTGTGGTCG GCCCCGTGGG AGCAGGGAAG TCATCACTGT 
     2401 TAAGTGCCGT GCTCGGGGAA TTGGCCCCAA GTCACGGGCT GGTCAGCGTG CATGGAAGAA 
     2461 TTGCCTATGT GTCTCAGCAG CCCTGGGTGT TCTCGGGAAC TCTGAGGAGT AATATTTTAT 
     2521 TTGGGAAGAA ATATGAAAAG GAACGATATG AAAAAGTCAT AAAGGCTTGT GCTCTGAAAA 
     2581 AGGATTTACA GCTGTTGGAG GATGGTGATC TGACTGTGAT AGGAGATCGG GGAACCACGC 
     2641 TGAGTGGAGG GCAGAAAGCA CGGGTAAACC TTGCAAGAGC AGTGTATCAA GATGCTGACA 
     2701 TCTATCTCCT GGACGATCCT CTCAGTGCAG TAGATGCGGA AGTTAGCAGA CACTTGTTCG 
     2761 AACTGTGTAT TTGTCAAATT TTGCATGAGA AGATCACAAT TTTAGTGACT CATCAGTTGC 
     2821 AGTACCTCAA AGCTGCAAGT CAGATTCTGA TATTGAAAGA TGGTAAAATG GTGCAGAAGG 
     2881 GGACTTACAC TGAGTTCCTA AAATCTGGTA TAGATTTTGG CTCCCTTTTA AAGAAGGATA 
     2941 ATGAGGAAAG TGAACAACCT CCAGTTCCAG GAACTCCCAC ACTAAGGAAT CGTACCTTCT 
     3001 CAGAGTCTTC GGTTTGGTCT CAACAATCTT CTAGACCCTC CTTGAAAGAT GGTGCTCTGG 
     3061 AGAGCCAAGA TACAGAGAAT GTCCCAGTTA CACTATCAGA GGAGAACCGT TCTGAAGGAA 
     3121 AAGTTGGTTT TCAGGCCTAT AAGAATTACT TCAGAGCTGG TGCTCACTGG ATTGTCTTCA 
     3181 TTTTCCTTAT TCTCCTAAAC ACTGCAGCTC AGGTTGCCTA TGTGCTTCAA GATTGGTGGC 
     3241 TTTCATACTG GGCAAACAAA CAAAGTATGC TAAATGTCAC TGTAAATGGA GGAGGAAATG 
     3301 TAACCGAGAA GCTAGATCTT AACTGGTACT TAGGAATTTA TTCAGGTTTA ACTGTAGCTA 
     3361 CCGTTCTTTT TGGCATAGCA AGATCTCTAT TGGTATTCTA CGTCCTTGTT AACTCTTCAC 
     3421 AAACTTTGCA CAACAAAATG TTTGAGTCAA TTCTGAAAGC TCCGGTATTA TTCTTTGATA 
     3481 GAAATCCAAT AGGAAGAATT TTAAATCGTT TCTCCAAAGA CATTGGACAC TTGGATGATT 
     3541 TGCTGCCGCT GACGTTTTTA GATTTCATCC AGACATTGCT ACAAGTGGTT GGTGTGGTCT 
     3601 CTGTGGCTGT GGCCGTGATT CCTTGGATCG CAATACCCTT GGTTCCCCTT GGAATCATTT 
     3661 TCATTTTTCT TCGGCGATAT TTTTTGGAAA CGTCAAGAGA TGTGAAGCGC CTGGAATCTA 
     3721 CAACTCGGAG TCCAGTGTTT TCCCACTTGT CATCTTCTCT CCAGGGGCTC TGGACCATCC 
     3781 GGGCATACAA AGCAGAAGAG AGGTGTCAGG AACTGTTTGA TGCACACCAG GATTTACATT 
     3841 CAGAGGCTTG GTTCTTGTTT TTGACAACGT CCCGCTGGTT CGCCGTCCGT CTGGATGCCA 
     3901 TCTGTGCCAT GTTTGTCATC ATCGTTGCCT TTGGGTCCCT GATTCTGGCA AAAACTCTGG 
     3961 ATGCCGGGCA GGTTGGTTTG GCACTGTCCT ATGCCCTCAC GCTCATGGGG ATGTTTCAGT 
     4021 GGTGTGTTCG ACAAAGTGCT GAAGTTGAGA ATATGATGAT CTCAGTAGAA AGGGTCATTG 
     4081 AATACACAGA CCTTGAAAAA GAAGCACCTT GGGAATATCA GAAACGCCCA CCACCAGCCT 
     4141 GGCCCCATGA AGGAGTGATA ATCTTTGACA ATGTGAACTT CATGTACAGT CCAGGTGGGC 
     4201 CTCTGGTACT GAAGCATCTG ACAGCACTCA TTAAATCACA AGAAAAGGTT GGCATTGTGG 
     4261 GAAGAACCGG AGCTGGAAAA AGTTCCCTCA TCTCAGCCCT TTTTAGATTG TCAGAACCCG 
     4321 AAGGTAAAAT TTGGATTGAT AAGATCTTGA CAACTGAAAT TGGACTTCAC GATTTAAGGA 
     4381 AGAAAATGTC AATCATACCT CAGGAACCTG TTTTGTTCAC TGGAACAATG AGGAAAAACC 
     4441 TGGATCCCTT TAAGGAGCAC ACGGATGAGG AACTGTGGAA TGCCTTACAA GAGGTACAAC 
     4501 TTAAAGAAAC CATTGAAGAT CTTCCTGGTA AAATGGATAC TGAATTAGCA GAATCAGGAT 
     4561 CCAATTTTAG TGTTGGACAA AGACAACTGG TGTGCCTTGC CAGGGCAATT CTCAGGAAAA 
     4621 ATCAGATATT GATTATTGAT GAAGCGACGG CAAATGTGGA TCCAAGAACT GATGAGTTAA 
     4681 TACAAAAAAA AATCCGGGAG AAATTTGCCC ACTGCACCGT GCTAACCATT GCACACAGAT 
     4741 TGAACACCAT TATTGACAGC GACAAGATAA TGGTTTTAGA TTCAGGAAGA CTGAAAGAAT 
     4801 ATGATGAGCC GTATGTTTTG CTGCAAAATA AAGAGAGCCT ATTTTACAAG ATGGTGCAAC 
     4861 AACTGGGCAA GGCAGAAGCC GCTGCCCTCA CTGAAACAGC AAAACAGGTA TACTTCAAAA 
     4921 GAAATTATCC ACATATTGGT CACACTGACC ACATGGTTAC AAACACTTCC AATGGACAGC 
     4981 CCTCGACCTT AACTATTTTC GAGACAGCAC TGAGCGGACC GACGCGTACG CGGCCGCTCG 
     5041 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     5101 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     5161 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     5221 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5281 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5341 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5401 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5461 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5521 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5581 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5641 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5701 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5761 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5821 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5881 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5941 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     6001 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     6061 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     6121 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     6181 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6241 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6301 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6361 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6421 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6481 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6541 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6601 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6661 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6721 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6781 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6841 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6901 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6961 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     7021 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     7081 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     7141 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     7201 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7261 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7321 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7381 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7441 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7501 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7561 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7621 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7681 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7741 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7801 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7861 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7921 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7981 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     8041 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     8101 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8161 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     8221 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8281 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8341 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8401 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8461 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8521 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8581 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8641 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8701 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8761 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8821 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//