LOCUS       RC222919                9177 bp    DNA     circular  SYN 
DEFINITION  WRN (Myc-DDK-tagged)-Human Werner syndrome, RecQ helicase-like (WRN)
ACCESSION   NM_000553
VERSION     NM_000553.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5334..5425 
                     /label="Myc-DDK"  
     misc_feature    5455..6040 
                     /label="PolyA signal"  
     misc_feature    5512..5531 
                     /label="Restriction Enzyme"  
     misc_feature    6316..6988 
                     /label="ColE1"  
     misc_feature    7418..8212 
                     /label="Kan/Neo"  
     misc_feature    8247..8549 
                     /label="SV40 ori"  
     misc_feature    8635..9112 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5316..5322 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5316 
                     /label="WRN"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTGAAAAA AAATTGGAAA CAACTGCACA GCAGCGGAAA TGTCCTGAAT 
     1081 GGATGAATGT GCAGAATAAA AGATGTGCTG TAGAAGAAAG AAAGGCATGT GTTCGGAAGA 
     1141 GTGTTTTTGA AGATGACCTC CCCTTCTTAG AATTCACTGG ATCCATTGTG TATAGTTACG 
     1201 ATGCTAGTGA TTGCTCTTTC CTGTCAGAAG ATATTAGCAT GAGTCTATCA GATGGGGATG 
     1261 TGGTGGGATT TGACATGGAG TGGCCACCAT TATACAATAG AGGGAAACTT GGCAAAGTTG 
     1321 CACTAATTCA GTTGTGTGTT TCTGAGAGCA AATGTTACTT GTTCCACGTT TCTTCCATGT 
     1381 CAGTTTTTCC CCAGGGATTA AAAATGTTGC TTGAAAATAA AGCAGTTAAA AAGGCAGGTG 
     1441 TAGGAATTGA AGGAGATCAG TGGAAACTTC TACGTGACTT TGATATCAAA TTGAAGAATT 
     1501 TTGTGGAGTT GACAGATGTT GCCAATAAAA AGCTGAAATG TACAGAGACC TGGAGCCTTA 
     1561 ACAGTCTGGT TAAACACCTC TTAGGTAAAC AGCTCCTGAA AGACAAGTCT ATCCGCTGTA 
     1621 GCAATTGGAG TAAATTTCCT CTCACTGAGG ACCAGAAACT GTATGCAGCC ACTGATGCTT 
     1681 ATGCTGGTTT TATTATTTAC CGAAATTTAG AGATTTTGGA TGATACTGTG CAAAGGTTTG 
     1741 CTATAAATAA AGAGGAAGAA ATCCTACTTA GCGACATGAA CAAACAGTTG ACTTCAATCT 
     1801 CTGAGGAAGT GATGGATCTG GCTAAGCATC TTCCTCATGC TTTCAGTAAA TTGGAAAACC 
     1861 CACGGAGGGT TTCTATCTTA CTAAAGGATA TTTCAGAAAA TCTATATTCA CTGAGGAGGA 
     1921 TGATAATTGG GTCTACTAAC ATTGAGACTG AACTGAGGCC CAGCAATAAT TTAAACTTAT 
     1981 TATCCTTTGA AGATTCAACT ACTGGGGGAG TACAACAGAA ACAAATTAGA GAACATGAAG 
     2041 TTTTAATTCA CGTTGAAGAT GAAACATGGG ACCCAACACT TGATCATTTA GCTAAACATG 
     2101 ATGGAGAAGA TGTACTTGGA AATAAAGTGG AACGAAAAGA AGATGGATTT GAAGATGGAG 
     2161 TAGAAGACAA CAAATTGAAA GAGAATATGG AAAGAGCTTG TTTGATGTCG TTAGATATTA 
     2221 CAGAACATGA ACTCCAAATT TTGGAACAGC AGTCTCAGGA AGAATATCTT AGTGATATTG 
     2281 CTTATAAATC TACTGAGCAT TTATCTCCCA ATGATAATGA AAACGATACG TCCTATGTAA 
     2341 TTGAGAGTGA TGAAGATTTA GAAATGGAGA TGCTTAAGCA TTTATCTCCC AATGATAATG 
     2401 AAAACGATAC GTCCTATGTA ATTGAGAGTG ATGAAGATTT AGAAATGGAG ATGCTTAAGT 
     2461 CTTTAGAAAA CCTCAATAGT GGCACGGTAG AACCAACTCA TTCTAAATGC TTAAAAATGG 
     2521 AAAGAAATCT GGGTCTTCCT ACTAAAGAAG AAGAAGAAGA TGATGAAAAT GAAGCTAATG 
     2581 AAGGGGAAGA AGATGATGAT AAGGACTTTT TGTGGCCAGC ACCCAATGAA GAGCAAGTTA 
     2641 CTTGCCTCAA GATGTACTTT GGCCATTCCA GTTTTAAACC AGTTCAGTGG AAAGTGATTC 
     2701 ATTCAGTATT AGAAGAAAGA AGAGATAATG TTGCTGTCAT GGCAACTGGA TATGGAAAGA 
     2761 GTTTGTGCTT CCAGTATCCA CCTGTTTATG TAGGCAAGAT TGGCCTTGTT ATCTCTCCCC 
     2821 TTATTTCTCT GATGGAAGAC CAAGTGCTAC AGCTTAAAAT GTCCAACATC CCAGCTTGCT 
     2881 TCCTTGGATC AGCACAGTCA GAAAATGTTC TAACAGATAT TAAATTAGGT AAATACCGGA 
     2941 TTGTATACGT AACTCCAGAA TACTGTTCAG GTAACATGGG CCTGCTCCAG CAACTTGAGG 
     3001 CTGATATTGG TATCACGCTC ATTGCTGTGG ATGAGGCTCA CTGTATTTCT GAGTGGGGGC 
     3061 ATGATTTTAG GGATTCATTC AGGAAGTTGG GCTCCCTAAA GACAGCACTG CCAATGGTTC 
     3121 CAATCGTTGC ACTTACTGCT ACTGCAAGTT CTTCAATCCG GGAAGACATT GTACGTTGCT 
     3181 TAAATCTGAG AAATCCTCAG ATCACCTGTA CTGGTTTTGA TCGACCAAAC CTGTATTTAG 
     3241 AAGTTAGGCG AAAAACAGGG AATATCCTTC AGGATCTGCA GCCATTTCTT GTCAAAACAA 
     3301 GTTCCCACTG GGAATTTGAA GGTCCAACAA TCATCTACTG TCCTTCTAGA AAAATGACAC 
     3361 AACAAGTTAC AGGTGAACTT AGGAAACTTA ATCTATCCTG TGGAACATAC CATGCGGGCA 
     3421 TGAGTTTTAG CACAAGGAAA GACATTCATC ATAGGTTTGT AAGAGATGAA ATTCAGTGTG 
     3481 TCATAGCTAC CATAGCTTTT GGAATGGGCA TTAATAAAGC TGACATTCGC CAAGTCATTC 
     3541 ATTACGGTGC TCCTAAGGAC ATGGAATCAT ATTATCAGGA GATTGGTAGA GCTGGTCGTG 
     3601 ATGGACTTCA AAGTTCTTGT CACGTCCTCT GGGCTCCTGC AGACATTAAC TTAAATAGGC 
     3661 ACCTTCTTAC TGAGATACGT AATGAGAAGT TTCGATTATA CAAATTAAAG ATGATGGCAA 
     3721 AGATGGAAAA ATATCTTCAT TCTAGCAGAT GTAGGAGACA AATCATCTTG TCTCATTTTG 
     3781 AGGACAAACA AGTACAAAAA GCCTCCTTGG GAATTATGGG AACTGAAAAA TGCTGTGATA 
     3841 ATTGCAGGTC CAGATTGGAT CATTGCTATT CCATGGATGA CTCAGAGGAT ACATCCTGGG 
     3901 ACTTTGGTCC ACAAGCATTT AAGCTTTTGT CTGCTGTGGA CATCTTAGGC GAAAAATTTG 
     3961 GAATTGGGCT TCCAATTTTA TTTCTCCGAG GATCTAATTC TCAGCGTCTT GCCGATCAAT 
     4021 ATCGCAGGCA CAGTTTATTT GGCACTGGCA AGGATCAAAC AGAGAGTTGG TGGAAGGCTT 
     4081 TTTCCCGTCA GCTGATCACT GAGGGATTCT TGGTAGAAGT TTCTCGGTAT AACAAATTTA 
     4141 TGAAGATTTG CGCCCTTACG AAAAAGGGTA GAAATTGGCT TCATAAAGCT AATACAGAAT 
     4201 CTCAGAGCCT CATCCTTCAA GCTAATGAAG AATTGTGTCC AAAGAAGTTT CTTCTGCCTA 
     4261 GTTCGAAAAC TGTATCTTCG GGCACCAAAG AGCATTGTTA TAATCAAGTA CCAGTTGAAT 
     4321 TAAGTACAGA GAAGAAGTCT AACTTGGAGA AGTTATATTC TTATAAACCA TGTGATAAGA 
     4381 TTTCTTCTGG GAGTAACATT TCTAAAAAAA GTATCATGGT ACAGTCACCA GAAAAAGCTT 
     4441 ACAGTTCCTC ACAGCCTGTT ATTTCGGCAC AAGAGCAGGA GACTCAGATT GTGTTATATG 
     4501 GCAAATTGGT AGAAGCTAGG CAGAAACATG CCAATAAAAT GGATGTTCCC CCAGCTATTC 
     4561 TGGCAACAAA CAAGATACTG GTGGATATGG CCAAAATGAG ACCAACTACG GTTGAAAACG 
     4621 TAAAAAGGAT TGATGGTGTT TCTGAAGGCA AAGCTGCCAT GTTGGCCCCT CTGTTGGAAG 
     4681 TCATCAAACA TTTCTGCCAA ACAAATAGTG TTCAGACAGA CCTCTTTTCA AGTACAAAAC 
     4741 CTCAAGAAGA ACAGAAGACG AGTCTGGTAG CAAAAAATAA AATATGCACA CTTTCACAGT 
     4801 CTATGGCCAT CACATACTCT TTATTCCAAG AAAAGAAGAT GCCTTTGAAG AGCATAGCTG 
     4861 AGAGCAGGAT TCTGCCTCTC ATGACAATTG GCATGCACTT ATCCCAAGCG GTGAAAGCTG 
     4921 GCTGCCCCCT TGATTTGGAG CGAGCAGGCC TGACTCCAGA GGTTCAGAAG ATTATTGCTG 
     4981 ATGTTATCCG AAACCCTCCC GTCAACTCAG ATATGAGTAA AATTAGCCTA ATCAGAATGT 
     5041 TAGTTCCTGA AAACATTGAC ACGTACCTTA TCCACATGGC AATTGAGATC CTTAAACATG 
     5101 GTCCTGACAG CGGACTTCAA CCTTCATGTG ATGTCAACAA AAGGAGATGT TTTCCCGGTT 
     5161 CTGAAGAGAT CTGTTCAAGT TCTAAGAGAA GCAAGGAAGA AGTAGGCATC AATACTGAGA 
     5221 CTTCATCTGC AGAGAGAAAG AGACGATTAC CTGTGTGGTT TGCCAAAGGA AGTGATACCA 
     5281 GCAAGAAATT AATGGACAAA ACGAAAAGGG GAGGTCTTTT TAGTACGCGT ACGCGGCCGC 
     5341 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     5401 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     5461 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     5521 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     5581 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     5641 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     5701 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5761 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5821 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5881 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5941 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     6001 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     6061 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     6121 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     6181 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     6241 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     6301 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     6361 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     6421 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     6481 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     6541 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     6601 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6661 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6721 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6781 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6841 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6901 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6961 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     7021 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     7081 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     7141 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     7201 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     7261 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     7321 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     7381 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     7441 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     7501 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     7561 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     7621 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7681 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7741 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7801 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7861 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7921 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7981 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     8041 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     8101 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     8161 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     8221 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     8281 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     8341 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     8401 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8461 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     8521 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     8581 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     8641 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8701 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8761 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8821 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8881 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8941 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     9001 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     9061 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     9121 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//