LOCUS       RC222917                8736 bp    DNA     circular  SYN 
DEFINITION  MYO6 (Myc-DDK-tagged)-Human myosin VI (MYO6)
ACCESSION   NM_004999
VERSION     NM_004999.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4893..4984 
                     /label="Myc-DDK"  
     misc_feature    5014..5599 
                     /label="PolyA signal"  
     misc_feature    5071..5090 
                     /label="Restriction Enzyme"  
     misc_feature    5875..6547 
                     /label="ColE1"  
     misc_feature    6977..7771 
                     /label="Kan/Neo"  
     misc_feature    7806..8108 
                     /label="SV40 ori"  
     misc_feature    8194..8671 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4875..4881 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4875 
                     /label="MYO6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGATGGA AAGCCCGTTT GGGCGCCACA CCCTACAGAT GGATTTCAGA 
     1081 TGGGCAATAT TGTGGATATT GGCCCCGACA GCTTAACAAT TGAACCCTTG AATCAGAAAG 
     1141 GCAAGACATT TTTGGCTCTC ATAAACCAAG TGTTTCCTGC AGAAGAGGAC AGTAAAAAAG 
     1201 ATGTGGAAGA TAACTGTTCA CTAATGTATT TAAATGAAGC CACACTGCTC CATAATATCA 
     1261 AAGTTCGATA TAGTAAAGAC AGAATTTATA CATATGTCGC CAACATTCTG ATTGCAGTGA 
     1321 ATCCATACTT TGACATACCT AAAATATATT CTTCAGAAGC AATAAAGTCA TATCAAGGAA 
     1381 AATCTCTTGG GACAAGACCA CCTCATGTCT TTGCAATTGC TGATAAAGCT TTTCGAGACA 
     1441 TGAAGGTGCT CAAGATGAGT CAGTCTATCA TTGTATCTGG AGAATCAGGA GCCGGCAAAA 
     1501 CAGAAAATAC AAAATTTGTT CTAAGATACC TGACTGAATC CTATGGAACA GGTCAAGATA 
     1561 TTGATGACAG AATTGTTGAA GCTAACCCAC TCCTAGAAGC CTTTGGAAAT GCGAAGACTG 
     1621 TTCGCAACAA TAATAGCAGT CGATTTGGGA AATTTGTAGA AATACATTTT AATGAAAAGA 
     1681 GCTCAGTTGT TGGAGGATTT GTTTCACATT ATCTCCTAGA GAAATCTAGG ATCTGTGTTC 
     1741 AAGGCAAAGA GGAAAGAAAT TATCATATCT TTTATAGGTT GTGTGCTGGT GCTTCTGAAG 
     1801 ATATTAGAGA AAAACTTCAT TTGAGTTCAC CAGATAATTT TCGGTATTTA AACCGAGGCT 
     1861 GCACTAGATA CTTTGCTAAC AAAGAAACTG ACAAACAGAT TTTACAGAAC CGCAAAAGTC 
     1921 CTGAGTACCT TAAGGCAGGT TCTATGAAAG ATCCTCTGCT AGATGACCAT GGTGATTTTA 
     1981 TTAGAATGTG CACGGCTATG AAAAAAATTG GTTTGGATGA TGAAGAAAAG CTTGATCTCT 
     2041 TCCGGGTAGT AGCTGGCGTC CTGCACCTTG GAAATATTGA TTTTGAGGAA GCTGGCAGCA 
     2101 CTTCAGGTGG TTGTAATCTG AAGAATAAAT CTGCTCAGTC TTTGGAATAT TGTGCTGAAT 
     2161 TACTGGGTTT GGACCAAGAT GATCTTCGAG TAAGTTTGAC CACAAGAGTC ATGCTAACAA 
     2221 CAGCAGGGGG CACCAAAGGA ACAGTTATAA AGGTACCTCT GAAAGTGGAG CAAGCAAACA 
     2281 ATGCTCGTGA TGCCCTGGCA AAGACAGTGT ATAGCCATCT TTTTGATCAT GTGGTAAACA 
     2341 GAGTAAATCA GTGTTTTCCT TTTGAAACAT CATCCTATTT TATTGGAGTC CTAGATATTG 
     2401 CTGGTTTTGA GTACTTTGAG CATAACAGTT TTGAACAATT TTGCATCAAC TATTGCAATG 
     2461 AAAAACTTCA ACAATTTTTT AATGAAAGGA TTCTGAAGGA GGAACAAGAA CTCTATCAAA 
     2521 AAGAAGGTTT AGGTGTTAAT GAAGTGCATT ATGTGGATAA TCAGGACTGT ATAGATTTAA 
     2581 TTGAAGCCAA ATTAGTGGGA ATACTGGATA TTTTGGATGA AGAAAATCGC CTTCCCCAGC 
     2641 CAAGTGATCA ACACTTTACA TCTGCAGTTC ACCAAAAGCA CAAGGATCAT TTTCGACTCA 
     2701 CTATTCCCAG AAAATCTAAG CTGGCAGTTC ATAGGAATAT CAGAGACGAC GAAGGCTTCA 
     2761 TTATCAGGCA TTTTGCGGGG GCAGTGTGCT ATGAAACAAC CCAGTTTGTG GAGAAAAATA 
     2821 ATGATGCTTT ACATATGTCT CTTGAATCCT TAATATGTGA ATCCAGAGAT AAGTTTATAC 
     2881 GGGAATTATT TGAATCATCC ACAAATAACA ACAAAGATAC TAAACAAAAA GCAGGAAAAC 
     2941 TTAGCTTCAT CAGCGTGGGA AACAAGTTTA AGACACAGTT AAATTTGCTT CTGGATAAAC 
     3001 TTCGAAGTAC TGGAGCAAGC TTTATTCGTT GCATCAAACC TAACTTAAAG ATGACAAGCC 
     3061 ACCACTTTGA AGGTGCTCAA ATTCTGTCTC AGCTTCAGTG TTCAGGGATG GTGTCTGTTT 
     3121 TGGACTTGAT GCAGGGTGGT TACCCATCAC GAGCTTCATT TCATGAACTC TACAACATGT 
     3181 ACAAAAAGTA TATGCCAGAT AAACTTGCAA GATTGGATCC AAGACTATTT TGTAAGGCTT 
     3241 TGTTTAAAGC TTTGGGCTTA AATGAAAATG ACTACAAGTT TGGGTTAACC AAAGTATTTT 
     3301 TTAGACCTGG CAAGTTTGCA GAATTTGATC AGATCATGAA GTCTGACCCT GACCACTTAG 
     3361 CAGAGTTGGT TAAAAGAGTC AATCACTGGC TCACATGCAG TCGCTGGAAG AAAGTTCAGT 
     3421 GGTGCTCACT CTCAGTCATC AAATTGAAAA ACAAAATAAA ATATCGAGCT GAAGCCTGCA 
     3481 TTAAAATGCA AAAAACTATT CGAATGTGGC TTTGCAAGAG GAGACACAAA CCTCGCATTG 
     3541 ATGGTCTGGT TAAGGTGGGC ACACTGAAAA AACGACTTGA TAAATTTAAT GAGGTAGTCA 
     3601 GTGTGTTGAA AGATGGAAAA CCCGAGATGA ATAAACAGAT CAAGAATCTG GAAATTTCTA 
     3661 TTGATACTTT GATGGCCAAA ATTAAGTCCA CTATGATGAC GCAGGAACAA ATCCAGAAAG 
     3721 AATATGATGC ACTGGTTAAA AGCTCAGAGG AACTCCTCAG TGCATTACAG AAAAAAAAAC 
     3781 AGCAGGAAGA GGAAGCAGAA AGGCTGAGGC GTATTCAAGA AGAAATGGAA AAGGAAAGAA 
     3841 AAAGACGTGA AGAAGACGAA AAACGTCGAA GAAAGGAAGA GGAGGAAAGG CGGATGAAAC 
     3901 TTGAGATGGA AGCAAAGAGA AAACAAGAAG AAGAAGAGAG AAAGAAAAGG GAAGATGATG 
     3961 AAAAACGCAT TCAAGCTGAA GTGGAGGCAC AGCTGGCCCG ACAGAAGGAG GAGGAATCCC 
     4021 AACAGCAAGC AGTTCTGGAG CAGGAGCGCA GGGACCGGGA GCTGGCCCTG AGGATTGCCC 
     4081 AGAGTGAAGC CGAGCTCATC AGTGATGAGG CCCAGGCCGA CCTGGCGCTG CGGAGAAATG 
     4141 ATGGAACAAG ACCCAAAATG ACACCGGAAC AAATGGCCAA AGAAATGTCA GAATTTTTGA 
     4201 GTAGAGGTCC TGCTGTACTA GCCACCAAAG CAGCTGCTGG TACTAAGAAA TATGATCTTA 
     4261 GTAAATGGAA ATATGCAGAA CTACGTGATA CCATCAATAC TTCTTGTGAT ATTGAGCTCC 
     4321 TGGCAGCTTG CAGAGAAGAA TTTCATAGGA GACTAAAAGT GTATCATGCT TGGAAATCTA 
     4381 AGAACAAGAA GAGAAATACT GAAACAGAGC AACGTGCTCC AAAGTCTGTT ACTGATTATG 
     4441 ATTTTGCACC ATTTTTGAAC AATTCACCTC AGCAAAACCC AGCAGCTCAG ATTCCTGCCA 
     4501 GGCAGCGGGA GATTGAAATG AACCGACAGC AACGCTTCTT CCGCATCCCA TTCATCCGCC 
     4561 CTGCCGACCA GTACAAAGAC CCTCAGAGTA AGAAAAAAGG CTGGTGGTAT GCCCATTTTG 
     4621 ATGGACCATG GATTGCCCGG CAAATGGAAC TCCATCCTGA CAAGCCACCC ATCCTACTTG 
     4681 TGGCTGGTAA GGACGACATG GAGATGTGTG AGCTGAATCT TGAGGAGACT GGCCTGACTC 
     4741 GGAAGCGTGG TGCTGAGATC TTGCCAAGAC AGTTTGAAGA AATCTGGGAA CGCTGTGGAG 
     4801 GCATCCAGTA CCTTCAGAAT GCGATTGAGA GCAGACAGGC TCGGCCCACC TATGCAACAG 
     4861 CCATGCTGCA GAGTCTGTTA AAGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4921 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4981 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     5041 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     5101 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     5161 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5221 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5281 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5341 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5401 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5461 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5521 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5581 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5641 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5701 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5761 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5821 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5881 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5941 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     6001 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     6061 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     6121 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6181 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6241 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6301 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6361 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6421 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6481 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6541 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6601 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6661 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6721 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6781 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6841 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6901 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6961 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     7021 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     7081 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     7141 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7201 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7261 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7321 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7381 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7441 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7501 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7561 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7621 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7681 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7741 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7801 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7861 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7921 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7981 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8041 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8101 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     8161 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8221 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8281 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8341 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8401 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8461 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8521 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8581 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8641 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8701 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//