LOCUS       RC222871                9198 bp    DNA     circular  SYN 
DEFINITION  PTPRU (Myc-DDK-tagged)-Human protein tyrosine phosphatase, receptor type, U (PTPRU), transcript variant 1
ACCESSION   NM_133178
VERSION     NM_133178.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5353..5444 
                     /label="Myc-DDK"  
     misc_feature    5474..6059 
                     /label="PolyA signal"  
     misc_feature    5531..5550 
                     /label="Restriction Enzyme"  
     misc_feature    6335..7007 
                     /label="ColE1"  
     misc_feature    7437..8231 
                     /label="Kan/Neo"  
     misc_feature    8266..8568 
                     /label="SV40 ori"  
     misc_feature    8654..9131 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5328..5335 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5328 
                     /label="PTPRU"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCGTGCC CAGGCGCTCG TGCTGGCACT CACCTTCCAG CTCTGCGCGC 
     1081 CGGAGACCGA GACTCCGGCA GCTGGCTGCA CCTTCGAGGA GGCAAGTGAC CCAGCAGTGC 
     1141 CCTGCGAGTA CAGCCAGGCC CAGTACGATG ACTTCCAGTG GGAGCAAGTG CGAATCCACC 
     1201 CTGGCACCCG GGCACCTGCG GACCTGCCCC ACGGCTCCTA CTTGATGGTC AACACTTCCC 
     1261 AGCATGCCCC AGGCCAGCGA GCCCATGTCA TCTTCCAGAG CCTGAGCGAG AATGATACCC 
     1321 ACTGTGTGCA GTTCAGCTAC TTCCTGTACA GCCGGGACGG GCACAGCCCG GGCACCCTGG 
     1381 GCGTCTACGT GCGCGTTAAT GGGGGCCCCC TGGGCAGTGC TGTGTGGAAT ATGACTGGAT 
     1441 CCCACGGCCG TCAGTGGCAC CAGGCTGAGC TGGCTGTCAG CACTTTCTGG CCCAATGAAT 
     1501 ATCAGGTGCT GTTTGAGGCC CTCATCTCCC CAGACCGCAG GGGCTACATG GGCCTAGATG 
     1561 ACATCCTGCT TCTCAGCTAC CCCTGCGCAA AGGCCCCACA CTTCTCCCGC CTGGGCGACG 
     1621 TGGAGGTCAA CGCGGGCCAG AACGCGTCGT TCCAGTGCAT GGCCGCGGGC AGAGCGGCCG 
     1681 AGGCCGAACG CTTCCTCTTG CAACGGCAGA GCGGGGCGCT GGTGCCGGCG GCGGGCGTGC 
     1741 GGCACATCAG CCACCGGCGC TTCCTGGCCA CTTTCCCGCT GGCTGCCGTG AGCCGCGCCG 
     1801 AGCAGGACCT GTACCGCTGT GTGTCCCAGG CCCCGCGCGG CGCGGGCGTC TCTAACTTCG 
     1861 CGGAGCTCAT CGTCAAGGAG CCCCCAACTC CCATCGCGCC CCCACAGCTG CTGCGTGCTG 
     1921 GCCCCACCTA CCTCATCATC CAGCTCAACA CCAACTCCAT CATTGGCGAC GGGCCGATCG 
     1981 TGCGCAAGGA GATTGAGTAC CGCATGGCGC GCGGGCCCTG GGCTGAGGTG CACGCCGTCA 
     2041 GCCTGCAGAC CTACAAGCTG TGGCACCTCG ACCCCGACAC AGAGTATGAG ATCAGCGTGC 
     2101 TGCTCACGCG TCCCGGAGAC GGCGGCACTG GCCGCCCTGG GCCACCCCTC ATCAGCCGCA 
     2161 CCAAATGCGC AGAGCCCATG AGGGCCCCCA AAGGCCTGGC TTTTGCTGAG ATCCAGGCCC 
     2221 GTCAGCTGAC CCTGCAGTGG GAACCACTGG GCTACAACGT GACGCGTTGC CACACCTATA 
     2281 CTGTGTCGCT GTGCTATCAC TACACCCTGG GCAGCAGCCA CAACCAGACC ATCCGAGAGT 
     2341 GTGTGAAGAC AGAGCAAGGT GTCAGCCGCT ACACCATCAA GAACCTGCTG CCCTATCGGA 
     2401 ACGTTCACGT GAGGCTTGTC CTCACTAACC CTGAGGGGCG CAAAGAGGGC AAGGAGGTCA 
     2461 CTTTCCAGAC GGATGAGGAT GTGCCCAGTG GGATTGCAGC CGAGTCCCTG ACCTTCACTC 
     2521 CACTGGAGGA CATGATCTTC CTCAAGTGGG AGGAGCCCCA GGAGCCCAAT GGTCTCATCA 
     2581 CCCAGTATGA GATCAGCTAC CAGAGCATCG AGTCATCAGA CCCGGCAGTG AACGTGCCAG 
     2641 GCCCACGACG TACCATCTCC AAGCTCCGCA ATGAGACCTA CCATGTCTTC TCCAACCTGC 
     2701 ACCCAGGCAC CACCTACCTG TTCTCCGTGC GGGCCCGCAC AGGCAAAGGC TTCGGCCAGG 
     2761 CGGCACTCAC TGAGATAACC ACTAACATCT CTGCTCCCAG CTTTGATTAT GCCGACATGC 
     2821 CGTCACCCCT GGGCGAGTCT GAGAACACCA TCACCGTGCT GCTGAGGCCG GCACAGGGCC 
     2881 GCGGTGCGCC CATCAGTGTG TACCAGGTGA TTGTGGAGGA GGAGCGGGCG CGGAGGCTGC 
     2941 GGCGGGAGCC AGGTGGACAG GACTGCTTCC CAGTGCCATT GACCTTCGAG GCGGCGCTGG 
     3001 CCCGAGGCCT GGTGCACTAC TTCGGGGCCG AACTGGCGGC CAGCAGTCTA CCTGAGGCCA 
     3061 TGCCCTTTAC CGTGGGTGAC AACCAGACCT ACCGAGGCTT CTGGAACCCA CCACTTGAGC 
     3121 CTAGGAAGGC CTATCTCATC TACTTCCAGG CAGCAAGCCA CCTGAAGGGG GAGACCCGGC 
     3181 TGAATTGCAT CCGCATTGCC AGGAAAGCTG CCTGCAAGGA AAGCAAGCGG CCCCTGGAGG 
     3241 TGTCCCAGAG ATCGGAGGAG ATGGGGCTTA TCCTGGGCAT CTGTGCAGGG GGGCTTGCTG 
     3301 TCCTCATCCT TCTCCTGGGT GCCATCATTG TCATCATCCG CAAAGGGAAG CCGGTGAACA 
     3361 TGACCAAGGC CACCGTCAAC TACCGCCAGG AGAAGACACA CATGATGAGC GCCGTGGACC 
     3421 GCAGCTTCAC AGACCAGAGC ACCCTGCAGG AGGACGAGCG GCTGGGCCTG TCCTTCATGG 
     3481 ACACCCATGG CTACAGCACC CGGGGAGACC AGCGCAGCGG TGGGGTCACT GAGGCCAGCA 
     3541 GCCTCCTGGG GGGCTCCCCG AGGCGTCCCT GTGGCCGGAA GGGCTCCCCA TACCACACGG 
     3601 GGCAGCTGCA CCCTGCGGTG CGTGTCGCAG ACCTTCTGCA GCACATCAAC CAGATGAAGA 
     3661 CGGCCGAGGG TTACGGCTTC AAGCAGGAGT ATGAGAGCTT CTTTGAAGGC TGGGACGCCA 
     3721 CAAAGAAGAA AGACAAGGTC AAGGGCAGCC GGCAGGAGCC AATGCCTGCC TATGATCGGC 
     3781 ACCGAGTGAA ACTGCACCCG ATGCTGGGAG ACCCCAATGC CGACTACATT AATGCCAACT 
     3841 ACATAGATGG TTACCACAGG TCAAACCACT TCATAGCCAC TCAAGGGCCG AAGCCTGAGA 
     3901 TGGTCTATGA CTTCTGGCGT ATGGTGTGGC AGGAGCACTG TTCCAGCATC GTCATGATCA 
     3961 CCAAGCTGGT CGAGGTGGGC AGGGTGAAAT GCTCACGGTA CTGGCCGGAG GACTCAGACA 
     4021 CCTACGGGGA CATCAAGATT ATGCTGGTGA AGACAGAGAC CCTGGCTGAG TATGTCGTGC 
     4081 GCACTTTTGC CCTGGAGCGG AGAGGCTACT CTGCCCGGCA CGAGGTCCGC CAGTTCCACT 
     4141 TCACAGCGTG GCCAGAGCAT GGCGTCCCCT ACCATGCCAC GGGGCTGCTG GCTTTCATCC 
     4201 GGCGCGTGAA GGCCTCCACC CCACCTGATG CCGGGCCCAT TGTCATCCAC TGCAGCGCGG 
     4261 GCACCGGCCG CACAGGTTGC TATATCGTCC TGGATGTGAT GCTGGACATG GCAGAGTGTG 
     4321 AGGGCGTCGT GGACATTTAC AACTGTGTGA AGACTCTCTG CTCCCGGCGT GTCAACATGA 
     4381 TCCAGACTGA GGAGCAGTAC ATCTTCATTC ATGATGCAAT CCTGGAGGCC TGCCTGTGTG 
     4441 GGGAGACCAC CATCCCTGTC AGTGAGTTCA AGGCCACCTA CAAGGAGATG ATCCGCATTG 
     4501 ATCCTCAGAG TAATTCCTCC CAGCTGCGGG AAGAGTTCCA GACGCTGAAC TCGGTCACCC 
     4561 CGCCGCTGGA CGTGGAGGAG TGCAGCATCG CCCTGTTGCC CCGGAACCGC GACAAGAACC 
     4621 GCAGCATGGA CGTCCTGCCG CCCGACCGCT GCCTGCCCTT CCTCATCTCC ACTGATGGGG 
     4681 ACTCCAACAA CTACATTAAT GCAGCCCTGA CTGACAGCTA CACACGGAGT GCGGCCTTCA 
     4741 TCGTGACCCT GCACCCGCTG CAGAGCACCA CGCCCGACTT CTGGCGGCTG GTCTACGATT 
     4801 ACGGGTGCAC CTCCATCGTC ATGCTCAACC AGCTGAACCA GTCCAACTCC GCCTGGCCCT 
     4861 GCCTGCAGTA CTGGCCAGAG CCAGGCCGGC AGCAATATGG CCTCATGGAG GTGGAGTTTA 
     4921 TGTCGGGCAC AGCTGATGAA GACTTAGTGG CTCGAGTCTT CCGGGTGCAG AACATCTCTC 
     4981 GGTTGCAGGA GGGGCACCTG CTGGTGCGGC ACTTCCAGTT CCTGCGCTGG TCTGCATACC 
     5041 GGGACACACC TGACTCCAAG AAGGCCTTCT TGCACCTGCT GGCTGAGGTG GACAAGTGGC 
     5101 AGGCCGAGAG TGGGGATGGG CGCACCATCG TGCACTGCCT AAACGGGGGA GGACGCAGCG 
     5161 GCACCTTCTG CGCCTGCGCC ACGGTCCTGG AGATGATCCG CTGCCACAAC TTGGTGGACG 
     5221 TTTTCTTTGC TGCCAAAACC CTCCGGAACT ACAAACCCAA CATGGTGGAG ACCATGGATC 
     5281 AGTACCACTT TTGCTACGAT GTGGCCCTGG AGTACTTGGA GGGGCTGGAG TCAAGAAGCG 
     5341 GACCGACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     5401 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     5461 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     5521 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     5581 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     5641 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5701 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5761 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5821 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5881 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5941 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     6001 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     6061 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     6121 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     6181 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     6241 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     6301 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     6361 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     6421 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     6481 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     6541 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     6601 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6661 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6721 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6781 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6841 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6901 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6961 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     7021 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     7081 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     7141 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     7201 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     7261 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     7321 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     7381 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     7441 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     7501 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     7561 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     7621 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7681 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7741 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7801 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7861 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7921 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7981 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     8041 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     8101 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     8161 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     8221 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     8281 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     8341 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     8401 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     8461 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8521 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8581 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     8641 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8701 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8761 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8821 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8881 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8941 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     9001 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     9061 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     9121 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     9181 AAATTTAACG CGAATTTT
//