LOCUS       RC222828                7482 bp    DNA     circular  SYN 
DEFINITION  SP140 (Myc-DDK-tagged)-Human SP140 nuclear body protein (SP140), transcript variant 1
ACCESSION   NM_007237
VERSION     NM_007237.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3639..3730 
                     /label="Myc-DDK"  
     misc_feature    3760..4345 
                     /label="PolyA signal"  
     misc_feature    3817..3836 
                     /label="Restriction Enzyme"  
     misc_feature    4621..5293 
                     /label="ColE1"  
     misc_feature    5723..6517 
                     /label="Kan/Neo"  
     misc_feature    6552..6854 
                     /label="SV40 ori"  
     misc_feature    6940..7417 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3621..3627 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3621 
                     /label="SP140"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCAGCAG GGCCAGCAGG GGCAGATGGC AAGTGGAGAC AGCAATCTCA 
     1081 ACTTCAGGAT GGTCGCAGAG ATCCAGAACG TAGAGGGTCA GAACCTGCAG GAGCAGGTTT 
     1141 GCCCTGAGCC CATTTTCAGG TTCTTCAGAG AAAACAAGGT GGAGATTGCA AGTGCAATAA 
     1201 CAAGGCCATT TCCTTTCCTT ATGGGCCTCC GAGACCGCTC CTTCATCTCC GAGCAGATGT 
     1261 ATGAACATTT TCAAGAAGCT TTTAGAAACC TGGTCCCAGT GACAAGAGTG ATGTATTGTG 
     1321 TACTCAGTGA ACTGGAGAAG ACATTTGGCT GGTCACATCT GGAAGCATTG TTCAGCAGGA 
     1381 TTAACCTGAT GGCCTATCCT GATTTAAACG AGATTTACAG AAGCTTCCAG AATGTATGCT 
     1441 ATGAACACTC ACCTCTCCAA ATGAATAATG TAAACGATTT AGAAGATAGA CCCAGATTAC 
     1501 TACCATATGG TAAACAAGAG AACAGCAATG CCTGTCATGA AATGGATGAT ATAGCAGTGC 
     1561 CTCAGGAAGC CTTGAGCTCC TCGCCAAGGT GTGAGCCAGG TTTCTCTTCA GAGTCTTGTG 
     1621 AGCAGTTAGC TCTCCCAAAG GCTGGTGGAG GAGATGCTGA AGATGCACCC AGCCTACTAC 
     1681 CAGGTGGGGG AGTGTCCTGT AAACTTGCTA TACAAATAGA TGAAGGAGAA TCAGAAGAAA 
     1741 TGCCCAAGTT ACTGCCTTAT GATACAGAAG TTCTAGAAAG CAACGGGATG ATAGATGCGG 
     1801 CAAGGACATA CAGCACAGCA CCAGGGGAGA AACAGGGAGA GGAGGAAGGC AGGAACAGTC 
     1861 CCAGAAAAAG AAACCAAGAC AAGGAGAAGT ACCAAGAGAG TCCAGAGGGA AGAGACAAAG 
     1921 AGACCTTTGA TCTAAAAACT CCCCAAGTCA CTAATGAAGG AGAACCAGAG AAGGGGCTCT 
     1981 GTCTACTACC AGGTGAAGGA GAAGAGGGCA GTGATGACTG TTCAGAAATG TGTGATGGAG 
     2041 AAGAGCCCCA GGAAGCCTCT AGCTCCCTAG CAAGATGTGG GTCAGTTTCT TGTTTATCTG 
     2101 CAGAGACCTT TGATCTAAAG ACTCCCCAAG TCACTAATGA AGGAGAACCA GAGAAGGAGC 
     2161 TCAGTCTACT ACCAGGTGAA GGAGAAGAGG GCAGTGATGA CTGTTCGGAA ATGTGTGATG 
     2221 GAGAAGAGCG CCAGGAAGCC TCTAGCTCCC TAGCAAGACG TGGGTCAGTG TCTAGTGAAC 
     2281 TAGAAAATCA CCCAATGAAT GAAGAAGGAG AATCAGAAGA GCTTGCTTCT AGCCTGCTAT 
     2341 ATGATAATGT ACCAGGAGCG GAGCAATCAG CATATGAAAA TGAGAAGTGT TCCTGTGTCA 
     2401 TGTGTTTCTC AGAAGAGGTG CCAGGAAGCC CAGAAGCAAG GACGGAAAGT GATCAAGCGT 
     2461 GTGGCACAAT GGATACTGTG GATATTGCAA ACAACTCCAC TTTGGGAAAA CCCAAGAGGA 
     2521 AAAGAAGAAA AAAGAGGGGG CATGGCTGGA GCAGAATGAG AATGAGAAGG CAGGAAAACA 
     2581 GCCAACAAAA TGATAATAGC AAAGCCGACG GCCAGGTGGT CTCCAGTGAA AAGAAGGCGA 
     2641 ACGTGAATCT GAAAGACCTT TCCAAGATTA GGGGGAGAAA GAGAGGCAAA CCTGGAACCC 
     2701 GCTTCACTCA GAGTGACAGA GCTGCACAGA AAAGAGTCCG ATCAAGAGCT TCAAGAAAGC 
     2761 ACAAAGATGA AACTGTGGAT TTTAAGGCTC CTTTGCTTCC AGTGACCTGT GGTGGGGTGA 
     2821 AGGGAATTTT ACATAAGAAG AAATTGCAGC AAGGAATCTT GGTGAAGTGT ATACAGACTG 
     2881 AGGATGGAAA ATGGTTCACC CCCACGGAAT TTGAAATCAA AGGAGGCCAT GCAAGATCAA 
     2941 AGAACTGGAG GCTGAGTGTG CGCTGTGGCG GGTGGCCCCT ACGATGGCTG ATGGAGAATG 
     3001 GATTTCTGCC TGATCCTCCA AGAATACGTT ACAGGAAAAA AAAGAGAATA CTGAAGTCTC 
     3061 AAAACAATAG CTCAGTTGAC CCTTGTATGA GAAACCTGGA TGAGTGTGAG GTGTGCCGGG 
     3121 ACGGAGGGGA GCTGTTCTGT TGCGACACTT GTTCAAGAGT CTTCCATGAG GACTGTCACA 
     3181 TCCCGCCTGT GGAAGCTGAG AGGACCCCGT GGAATTGCAT CTTCTGCAGG ATGAAGGAGT 
     3241 CTCCGGGAAG CCAACAGTGT TGTCAGGAAT CTGAGGTCCT GGAGAGGCAG ATGTGTCCTG 
     3301 AGGAACAGTT GAAATGTGAG TTCCTCCTCT TGAAAGTCTA TTGCTGTTCT GAGAGCTCCT 
     3361 TTTTTGCCAA GATTCCATAC TATTATTATA TTAGAGAGGC GTGTCAAGGC CTGAAGGAGC 
     3421 CCATGTGGTT GGATAAAATC AAGAAAAGGC TGAATGAGCA CGGTTACCCC CAAGTGGAGG 
     3481 GGTTTGTACA AGACATGCGC CTCATCTTCC AGAACCACAG GGCCTCTTAC AAGTACAAGG 
     3541 ATTTTGGCCA AATGGGATTT AGACTGGAGG CTGAGTTTGA GAAGAATTTC AAGGAAGTGT 
     3601 TTGCTATTCA GGAAACAAAT GGGAACAATA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     3661 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     3721 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     3781 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     3841 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     3901 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     3961 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4021 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4081 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4141 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4201 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4261 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4321 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4381 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     4441 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     4501 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     4561 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     4621 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     4681 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     4741 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     4801 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     4861 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     4921 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     4981 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5041 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5101 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     5161 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     5221 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     5281 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     5341 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     5401 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     5461 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     5521 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     5581 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     5641 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     5701 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     5761 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     5821 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     5881 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     5941 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6001 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6061 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6121 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     6181 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6241 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     6301 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     6361 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     6421 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     6481 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     6541 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     6601 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     6661 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     6721 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6781 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6841 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     6901 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     6961 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7021 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7081 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7141 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     7201 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7261 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     7321 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     7381 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     7441 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//