LOCUS RC222820 7398 bp DNA circular SYN DEFINITION TLR4 (Myc-DDK-tagged)-Human toll-like receptor 4 (TLR4), transcript variant 1 ACCESSION NM_138554 VERSION null SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 3555..3646 /label="Myc-DDK" misc_feature 3676..4261 /label="PolyA signal" misc_feature 3733..3752 /label="Restriction Enzyme" misc_feature 4537..5209 /label="ColE1" misc_feature 5639..6433 /label="Kan/Neo" misc_feature 6468..6770 /label="SV40 ori" misc_feature 6856..7333 /label="f1 ori" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 3537..3543 /label="Restriction Enzyme" misc_feature 1020..3537 /label="TLR4" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GATGTCTGCC TCGCGCCTGG CTGGGACTCT GATCCCAGCC ATGGCCTTCC 1081 TCTCCTGCGT GAGACCAGAA AGCTGGGAGC CCTGCGTGGA GGTGGTTCCT AATATTACTT 1141 ATCAATGCAT GGAGCTGAAT TTCTACAAAA TCCCCGACAA CCTCCCCTTC TCAACCAAGA 1201 ACCTGGACCT GAGCTTTAAT CCCCTGAGGC ATTTAGGCAG CTATAGCTTC TTCAGTTTCC 1261 CAGAACTGCA GGTGCTGGAT TTATCCAGGT GTGAAATCCA GACAATTGAA GATGGGGCAT 1321 ATCAGAGCCT AAGCCACCTC TCTACCTTAA TATTGACAGG AAACCCCATC CAGAGTTTAG 1381 CCCTGGGAGC CTTTTCTGGA CTATCAAGTT TACAGAAGCT GGTGGCTGTG GAGACAAATC 1441 TAGCATCTCT AGAGAACTTC CCCATTGGAC ATCTCAAAAC TTTGAAAGAA CTTAATGTGG 1501 CTCACAATCT TATCCAATCT TTCAAATTAC CTGAGTATTT TTCTAATCTG ACCAATCTAG 1561 AGCACTTGGA CCTTTCCAGC AACAAGATTC AAAGTATTTA TTGCACAGAC TTGCGGGTTC 1621 TACATCAAAT GCCCCTACTC AATCTCTCTT TAGACCTGTC CCTGAACCCT ATGAACTTTA 1681 TCCAACCAGG TGCATTTAAA GAAATTAGGC TTCATAAGCT GACTTTAAGA AATAATTTTG 1741 ATAGTTTAAA TGTAATGAAA ACTTGTATTC AAGGTCTGGC TGGTTTAGAA GTCCATCGTT 1801 TGGTTCTGGG AGAATTTAGA AATGAAGGAA ACTTGGAAAA GTTTGACAAA TCTGCTCTAG 1861 AGGGCCTGTG CAATTTGACC ATTGAAGAAT TCCGATTAGC ATACTTAGAC TACTACCTCG 1921 ATGATATTAT TGACTTATTT AATTGTTTGA CAAATGTTTC TTCATTTTCC CTGGTGAGTG 1981 TGACTATTGA AAGGGTAAAA GACTTTTCTT ATAATTTCGG ATGGCAACAT TTAGAATTAG 2041 TTAACTGTAA ATTTGGACAG TTTCCCACAT TGAAACTCAA ATCTCTCAAA AGGCTTACTT 2101 TCACTTCCAA CAAAGGTGGG AATGCTTTTT CAGAAGTTGA TCTACCAAGC CTTGAGTTTC 2161 TAGATCTCAG TAGAAATGGC TTGAGTTTCA AAGGTTGCTG TTCTCAAAGT GATTTTGGGA 2221 CAACCAGCCT AAAGTATTTA GATCTGAGCT TCAATGGTGT TATTACCATG AGTTCAAACT 2281 TCTTGGGCTT AGAACAACTA GAACATCTGG ATTTCCAGCA TTCCAATTTG AAACAAATGA 2341 GTGAGTTTTC AGTATTCCTA TCACTCAGAA ACCTCATTTA CCTTGACATT TCTCATACTC 2401 ACACCAGAGT TGCTTTCAAT GGCATCTTCA ATGGCTTGTC CAGTCTCGAA GTCTTGAAAA 2461 TGGCTGGCAA TTCTTTCCAG GAAAACTTCC TTCCAGATAT CTTCACAGAG CTGAGAAACT 2521 TGACCTTCCT GGACCTCTCT CAGTGTCAAC TGGAGCAGTT GTCTCCAACA GCATTTAACT 2581 CACTCTCCAG TCTTCAGGTA CTAAATATGA GCCACAACAA CTTCTTTTCA TTGGATACGT 2641 TTCCTTATAA GTGTCTGAAC TCCCTCCAGG TTCTTGATTA CAGTCTCAAT CACATAATGA 2701 CTTCCAAAAA ACAGGAACTA CAGCATTTTC CAAGTAGTCT AGCTTTCTTA AATCTTACTC 2761 AGAATGACTT TGCTTGTACT TGTGAACACC AGAGTTTCCT GCAATGGATC AAGGACCAGA 2821 GGCAGCTCTT GGTGGAAGTT GAACGAATGG AATGTGCAAC ACCTTCAGAT AAGCAGGGCA 2881 TGCCTGTGCT GAGTTTGAAT ATCACCTGTC AGATGAATAA GACCATCATT GGTGTGTCGG 2941 TCCTCAGTGT GCTTGTAGTA TCTGTTGTAG CAGTTCTGGT CTATAAGTTC TATTTTCACC 3001 TGATGCTTCT TGCTGGCTGC ATAAAGTATG GTAGAGGTGA AAACATCTAT GATGCCTTTG 3061 TTATCTACTC AAGCCAGGAT GAGGACTGGG TAAGGAATGA GCTAGTAAAG AATTTAGAAG 3121 AAGGGGTGCC TCCATTTCAG CTCTGCCTTC ACTACAGAGA CTTTATTCCC GGTGTGGCCA 3181 TTGCTGCCAA CATCATCCAT GAAGGTTTCC ATAAAAGCCG AAAGGTGATT GTTGTGGTGT 3241 CCCAGCACTT CATCCAGAGC CGCTGGTGTA TCTTTGAATA TGAGATTGCT CAGACCTGGC 3301 AGTTTCTGAG CAGTCGTGCT GGTATCATCT TCATTGTCCT GCAGAAGGTG GAGAAGACCC 3361 TGCTCAGGCA GCAGGTGGAG CTGTACCGCC TTCTCAGCAG GAACACTTAC CTGGAGTGGG 3421 AGGACAGTGT CCTGGGGCGG CACATCTTCT GGAGACGACT CAGAAAAGCC CTGCTGGATG 3481 GTAAATCATG GAATCCAGAA GGAACAGTGG GTACAGGATG CAATTGGCAG GAAGCAACAT 3541 CTATCACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 3601 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 3661 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 3721 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 3781 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 3841 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 3901 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 3961 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 4021 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 4081 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 4141 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 4201 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 4261 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 4321 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 4381 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 4441 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 4501 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 4561 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 4621 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 4681 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 4741 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 4801 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 4861 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 4921 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 4981 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 5041 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 5101 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 5161 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 5221 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 5281 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 5341 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 5401 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 5461 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 5521 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 5581 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 5641 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 5701 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 5761 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 5821 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 5881 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 5941 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 6001 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 6061 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 6121 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 6181 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 6241 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 6301 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 6361 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 6421 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 6481 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 6541 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 6601 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 6661 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 6721 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 6781 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 6841 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 6901 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 6961 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 7021 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 7081 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 7141 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 7201 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 7261 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 7321 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 7381 AAATTTAACG CGAATTTT //