LOCUS       RC222814                8412 bp    DNA     circular  SYN 
DEFINITION  ATP11B (Myc-DDK-tagged)-Human ATPase, class VI, type 11B (ATP11B)
ACCESSION   NM_014616
VERSION     NM_014616.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4569..4660 
                     /label="Myc-DDK"  
     misc_feature    4690..5275 
                     /label="PolyA signal"  
     misc_feature    4747..4766 
                     /label="Restriction Enzyme"  
     misc_feature    5551..6223 
                     /label="ColE1"  
     misc_feature    6653..7447 
                     /label="Kan/Neo"  
     misc_feature    7482..7784 
                     /label="SV40 ori"  
     misc_feature    7870..8347 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4551..4557 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4551 
                     /label="ATP11B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGGCGCTGG ATCCGGCAGC AGCTGGGTTT TGACCCACCA CATCAGAGTG 
     1081 ACACAAGAAC CATCTACGTA GCCAACAGGT TTCCTCAGAA TGGCCTTTAC ACACCTCAGA 
     1141 AATTTATAGA TAACAGGATC ATTTCATCTA AGTACACTGT GTGGAATTTT GTTCCAAAAA 
     1201 ATTTATTTGA ACAGTTCAGA AGAGTGGCAA ACTTTTATTT TCTTATTATA TTTTTGGTTC 
     1261 AGCTTATGAT TGATACACCT ACCAGTCCAG TTACCAGTGG ACTTCCATTA TTCTTTGTGA 
     1321 TAACAGTAAC TGCCATAAAG CAGGGATATG AAGATTGGTT ACGGCATAAC TCAGATAATG 
     1381 AAGTAAATGG AGCTCCTGTT TATGTTGTTC GAAGTGGTGG CCTTGTAAAA ACTAGATCAA 
     1441 AAAACATTCG GGTGGGTGAT ATTGTTCGAA TAGCCAAAGA TGAAATTTTT CCTGCAGACT 
     1501 TGGTGCTTCT GTCCTCAGAT CGACTGGATG GTTCCTGTCA CGTTACAACT GCTAGTTTGG 
     1561 ACGGAGAAAC TAACCTGAAG ACACATGTGG CAGTTCCAGA AACAGCATTA TTACAAACAG 
     1621 TTGCCAATTT GGACACTCTA GTAGCTGTAA TAGAATGCCA GCAACCAGAA GCAGACTTAT 
     1681 ACAGATTCAT GGGACGAATG ATCATAACCC AACAAATGGA AGAAATTGTA AGACCTCTGG 
     1741 GGCCGGAGAG TCTCCTGCTT CGTGGAGCCA GATTAAAAAA CACAAAAGAA ATTTTTGGTG 
     1801 TTGCGGTATA CACTGGAATG GAAACTAAGA TGGCATTAAA TTACAAGAGC AAATCACAGA 
     1861 AACGATCTGC AGTAGAAAAG TCAATGAATA CATTTTTGAT AATTTATCTA GTAATTCTTA 
     1921 TATCTGAAGC TGTCATCAGC ACTATCTTGA AGTATACATG GCAAGCTGAA GAAAAATGGG 
     1981 ATGAACCTTG GTATAACCAA AAAACAGAAC ATCAAAGAAA TAGCAGTAAG ATTCTGAGAT 
     2041 TTATTTCAGA CTTCCTTGCT TTTTTGGTTC TCTACAATTT CATCATTCCA ATTTCATTAT 
     2101 ATGTGACAGT CGAAATGCAG AAATTTCTTG GATCATTTTT TATTGGCTGG GATCTTGATC 
     2161 TGTATCATGA AGAATCAGAT CAGAAAGCTC AAGTCAATAC TTCCGATCTG AATGAAGAGC 
     2221 TTGGACAGGT AGAGTACGTG TTTACAGATA AAACTGGTAC ACTGACAGAA AATGAGATGC 
     2281 AGTTTCGGGA ATGTTCAATT AATGGCATGA AATACCAAGA AATTAATGGT AGACTTGTAC 
     2341 CCGAAGGACC AACACCAGAC TCTTCAGAAG GAAACTTATC TTATCTTAGT AGTTTATCCC 
     2401 ATCTTAACAA CTTATCCCAT CTTACAACCA GTTCCTCTTT CAGAACCAGT CCTGAAAATG 
     2461 AAACTGAACT AATTAAAGAA CATGATCTCT TCTTTAAAGC AGTCAGTCTC TGTCACACTG 
     2521 TACAGATTAG CAATGTTCAA ACTGACTGCA CTGGTGATGG TCCCTGGCAA TCCAACCTGG 
     2581 CACCATCGCA GTTGGAGTAC TATGCATCTT CACCAGATGA AAAGGCTCTA GTAGAAGCTG 
     2641 CTGCAAGGAT TGGTATTGTG TTTATTGGCA ATTCTGAAGA AACTATGGAG GTTAAAACTC 
     2701 TTGGAAAACT GGAACGGTAC AAACTGCTTC ATATTCTGGA ATTTGATTCA GATCGTAGGA 
     2761 GAATGAGTGT AATTGTTCAG GCACCTTCAG GTGAGAAGTT ATTATTTGCT AAAGGAGCTG 
     2821 AGTCATCAAT TCTCCCTAAA TGTATAGGTG GAGAAATAGA AAAAACCAGA ATTCATGTAG 
     2881 ATGAATTTGC TTTGAAAGGG CTAAGAACTC TGTGTATAGC ATATAGAAAA TTTACATCAA 
     2941 AAGAGTATGA GGAAATAGAT AAACGCATAT TTGAAGCCAG GACTGCCTTG CAGCAGCGGG 
     3001 AAGAGAAATT GGCAGCTGTT TTCCAGTTCA TAGAGAAAGA CCTGATATTA CTTGGAGCCA 
     3061 CAGCAGTAGA AGACAGACTA CAAGATAAAG TTCGAGAAAC TATTGAAGCA TTGAGAATGG 
     3121 CTGGTATCAA AGTATGGGTA CTTACTGGGG ATAAACATGA AACAGCTGTT AGTGTGAGTT 
     3181 TATCATGTGG CCATTTTCAT AGAACCATGA ACATCCTTGA ACTTATAAAC CAGAAATCAG 
     3241 ACAGCGAGTG TGCTGAACAA TTGAGGCAGC TTGCCAGAAG AATTACAGAG GATCATGTGA 
     3301 TTCAGCATGG GCTGGTAGTG GATGGGACCA GCCTATCTCT TGCACTCAGG GAGCATGAAA 
     3361 AACTATTTAT GGAAGTTTGC AGAAATTGTT CAGCTGTATT ATGCTGTCGT ATGGCTCCAC 
     3421 TGCAGAAAGC AAAAGTAATA AGACTAATAA AAATATCACC TGAGAAACCT ATAACATTGG 
     3481 CTGTTGGTGA TGGTGCTAAT GACGTAAGCA TGATACAAGA AGCCCATGTT GGCATAGGAA 
     3541 TCATGGGTAA AGAAGGAAGA CAGGCTGCAA GAAACAGTGA CTATGCAATA GCCAGATTTA 
     3601 AGTTCCTCTC CAAATTGCTT TTTGTTCATG GTCATTTTTA TTATATTAGA ATAGCTACCC 
     3661 TTGTACAGTA TTTTTTTTAT AAGAATGTGT GCTTTATCAC ACCCCAGTTT TTATATCAGT 
     3721 TCTACTGTTT GTTTTCTCAG CAAACATTGT ATGACAGCGT GTACCTGACT TTATACAATA 
     3781 TTTGTTTTAC TTCCCTACCT ATTCTGATAT ATAGTCTTTT GGAACAGCAT GTAGACCCTC 
     3841 ATGTGTTACA AAATAAGCCC ACCCTTTATC GAGACATTAG TAAAAACCGC CTCTTAAGTA 
     3901 TTAAAACATT TCTTTATTGG ACCATCCTGG GCTTCAGTCA TGCCTTTATT TTCTTTTTTG 
     3961 GATCCTATTT ACTAATAGGG AAAGATACAT CTCTGCTTGG AAATGGCCAG ATGTTTGGAA 
     4021 ACTGGACATT TGGCACTTTG GTCTTCACAG TCATGGTTAT TACAGTCACA GTAAAGATGG 
     4081 CTCTGGAAAC TCATTTTTGG ACTTGGATCA ACCATCTCGT TACCTGGGGA TCTATTATAT 
     4141 TTTATTTTGT ATTTTCCTTG TTTTATGGAG GGATTCTCTG GCCATTTTTG GGCTCCCAGA 
     4201 ATATGTATTT TGTGTTTATT CAGCTCCTGT CAAGTGGTTC TGCTTGGTTT GCCATAATCC 
     4261 TCATGGTTGT TACATGTCTA TTTCTTGATA TCATAAAGAA GGTCTTTGAC CGACACCTCC 
     4321 ACCCTACAAG TACTGAAAAG GCACAGCTTA CTGAAACAAA TGCAGGTATC AAGTGCTTGG 
     4381 ACTCCATGTG CTGTTTCCCG GAAGGAGAAG CAGCGTGTGC ATCTGTTGGA AGAATGCTGG 
     4441 AACGAGTTAT AGGAAGATGT AGTCCAACCC ACATCAGCAG ATCATGGAGT GCATCGGATC 
     4501 CTTTCTATAC CAACGACAGG AGCATCTTGA CTCTCTCCAC AATGGACTCA TCTACTTGTA 
     4561 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4621 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4681 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4741 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4801 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4861 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4921 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4981 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     5041 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     5101 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5161 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5221 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5281 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5341 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5401 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5461 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5521 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5581 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5641 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5701 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     5761 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5821 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     5881 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     5941 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     6001 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     6061 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6121 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6181 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6241 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6301 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6361 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6421 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6481 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6541 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6601 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6661 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6721 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6781 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6841 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     6901 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     6961 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     7021 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     7081 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7141 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7201 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7261 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7321 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7381 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7441 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7501 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7561 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7621 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7681 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7741 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7801 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7861 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     7921 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     7981 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     8041 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     8101 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     8161 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8221 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8281 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8341 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8401 AACGCGAATT TT
//