LOCUS       RC222754                7872 bp    DNA     circular  SYN 
DEFINITION  ATP2A2 (Myc-DDK-tagged)-Human ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 (ATP2A2), transcript variant 2
ACCESSION   NM_001681
VERSION     NM_001681.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4029..4120 
                     /label="Myc-DDK"  
     misc_feature    4150..4735 
                     /label="PolyA signal"  
     misc_feature    4207..4226 
                     /label="Restriction Enzyme"  
     misc_feature    5011..5683 
                     /label="ColE1"  
     misc_feature    6113..6907 
                     /label="Kan/Neo"  
     misc_feature    6942..7244 
                     /label="SV40 ori"  
     misc_feature    7330..7807 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4011..4017 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4011 
                     /label="ATP2A2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGAACGCG CACACCAAGA CGGTGGAGGA GGTGCTGGGC CACTTCGGCG 
     1081 TCAACGAGAG TACGGGGCTG AGCCTGGAAC AGGTCAAGAA GCTTAAGGAG AGATGGGGCT 
     1141 CCAACGAGTT ACCGGCTGAA GAAGGAAAAA CCTTGCTGGA ACTTGTGATT GAGCAGTTTG 
     1201 AAGACTTGCT AGTTAGGATT TTATTACTGG CAGCATGTAT ATCTTTTGTT TTGGCTTGGT 
     1261 TTGAAGAAGG TGAAGAAACA ATTACAGCCT TTGTAGAACC TTTTGTAATT TTACTCATAT 
     1321 TAGTAGCCAA TGCAATTGTG GGTGTATGGC AGGAAAGAAA TGCTGAAAAT GCCATCGAAG 
     1381 CCCTTAAGGA ATATGAGCCT GAAATGGGCA AAGTGTATCG ACAGGACAGA AAGAGTGTGC 
     1441 AGCGGATTAA AGCTAAAGAC ATAGTTCCTG GTGATATTGT AGAAATTGCT GTTGGTGACA 
     1501 AAGTTCCTGC TGATATAAGG TTAACTTCCA TCAAATCTAC CACACTAAGA GTTGACCAGT 
     1561 CAATTCTCAC AGGTGAATCT GTCTCTGTCA TCAAGCACAC TGATCCCGTC CCTGACCCAC 
     1621 GAGCTGTCAA CCAAGATAAA AAGAACATGC TGTTTTCTGG TACAAACATT GCTGCTGGGA 
     1681 AAGCTATGGG AGTGGTGGTA GCAACTGGAG TTAACACCGA AATTGGCAAG ATCCGGGATG 
     1741 AAATGGTGGC AACAGAACAG GAGAGAACAC CCCTTCAGCA AAAACTAGAT GAATTTGGGG 
     1801 AACAGCTTTC CAAAGTCATC TCCCTTATTT GCATTGCAGT CTGGATCATA AATATTGGGC 
     1861 ACTTCAATGA CCCGGTTCAT GGAGGGTCCT GGATCAGAGG TGCTATTTAC TACTTTAAAA 
     1921 TTGCAGTGGC CCTGGCTGTA GCAGCCATTC CTGAAGGTCT GCCTGCAGTC ATCACCACCT 
     1981 GCCTGGCTCT TGGAACTCGC AGAATGGCAA AGAAAAATGC CATTGTTCGA AGCCTCCCGT 
     2041 CTGTGGAAAC CCTTGGTTGT ACTTCTGTTA TCTGCTCAGA CAAGACTGGT ACACTTACAA 
     2101 CAAACCAGAT GTCAGTCTGC AGGATGTTCA TTCTGGACAG AGTGGAAGGT GATACTTGTT 
     2161 CCCTTAATGA GTTTACCATA ACTGGATCAA CTTATGCACC TATTGGAGAA GTGCATAAAG 
     2221 ATGATAAACC AGTGAATTGT CACCAGTATG ATGGTCTGGT AGAATTAGCA ACAATTTGTG 
     2281 CTCTTTGTAA TGACTCTGCT TTGGATTACA ATGAGGCAAA GGGTGTGTAT GAAAAAGTTG 
     2341 GAGAAGCTAC AGAGACTGCT CTCACTTGCC TAGTAGAGAA GATGAATGTA TTTGATACCG 
     2401 AATTGAAGGG TCTTTCTAAA ATAGAACGTG CAAATGCCTG CAACTCAGTC ATTAAACAGC 
     2461 TGATGAAAAA GGAATTCACT CTAGAGTTTT CACGTGACAG AAAGTCAATG TCGGTTTACT 
     2521 GTACACCAAA TAAACCAAGC AGGACATCAA TGAGCAAGAT GTTTGTGAAG GGTGCTCCTG 
     2581 AAGGTGTCAT TGACAGGTGC ACCCACATTC GAGTTGGAAG TACTAAGGTT CCTATGACCT 
     2641 CTGGAGTCAA ACAGAAGATC ATGTCTGTCA TTCGAGAGTG GGGTAGTGGC AGCGACACAC 
     2701 TGCGATGCCT GGCCCTGGCC ACTCATGACA ACCCACTGAG AAGAGAAGAA ATGCACCTTG 
     2761 AGGACTCTGC CAACTTTATT AAATATGAGA CCAATCTGAC CTTCGTTGGC TGCGTGGGCA 
     2821 TGCTGGATCC TCCGAGAATC GAGGTGGCCT CCTCCGTGAA GCTGTGCCGG CAAGCAGGCA 
     2881 TCCGGGTCAT CATGATCACT GGGGACAACA AGGGCACTGC TGTGGCCATC TGTCGCCGCA 
     2941 TCGGCATCTT CGGGCAGGAT GAGGACGTGA CGTCAAAAGC TTTCACAGGC CGGGAGTTTG 
     3001 ATGAACTCAA CCCCTCCGCC CAGCGAGACG CCTGCCTGAA CGCCCGCTGT TTTGCTCGAG 
     3061 TTGAACCCTC CCACAAGTCT AAAATCGTAG AATTTCTTCA GTCTTTTGAT GAGATTACAG 
     3121 CTATGACTGG CGATGGCGTG AACGATGCTC CTGCTCTGAA GAAAGCCGAG ATTGGCATTG 
     3181 CTATGGGCTC TGGCACTGCG GTGGCTAAAA CCGCCTCTGA GATGGTCCTG GCGGATGACA 
     3241 ACTTCTCCAC CATTGTGGCT GCCGTTGAGG AGGGGCGGGC AATCTACAAC AACATGAAAC 
     3301 AGTTCATCCG CTACCTCATC TCGTCCAACG TCGGGGAAGT TGTCTGTATT TTCCTGACAG 
     3361 CAGCCCTTGG ATTTCCCGAG GCTTTGATTC CTGTTCAGCT GCTCTGGGTC AATCTGGTGA 
     3421 CAGATGGCCT GCCTGCCACT GCACTGGGGT TCAACCCTCC TGATCTGGAC ATCATGAATA 
     3481 AACCTCCCCG GAACCCAAAG GAACCATTGA TCAGCGGGTG GCTCTTTTTC CGTTACTTGG 
     3541 CTATTGGCTG TTACGTCGGC GCTGCTACCG TGGGTGCTGC TGCATGGTGG TTCATTGCTG 
     3601 CTGACGGTGG TCCAAGAGTG TCCTTCTACC AGCTGAGTCA TTTCCTACAG TGTAAAGAGG 
     3661 ACAACCCGGA CTTTGAAGGC GTGGATTGTG CAATCTTTGA ATCCCCATAC CCGATGACAA 
     3721 TGGCGCTCTC TGTTCTAGTA ACTATAGAAA TGTGTAACGC CCTCAACAGC TTGTCCGAAA 
     3781 ACCAGTCCTT GCTGAGGATG CCCCCCTGGG AGAACATCTG GCTCGTGGGC TCCATCTGCC 
     3841 TGTCCATGTC ACTCCACTTC CTGATCCTCT ATGTCGAACC CTTGCCACTC ATCTTCCAGA 
     3901 TCACACCGCT GAACGTGACC CAGTGGCTGA TGGTGCTGAA AATCTCCTTG CCCGTGATTC 
     3961 TCATGGATGA GACGCTCAAG TTTGTGGCCC GCAACTACCT GGAACCTGCA ATACTGGAGA 
     4021 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4081 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4141 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4201 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4261 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4321 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4381 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4441 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4501 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4561 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     4621 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     4681 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     4741 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     4801 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     4861 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     4921 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     4981 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5041 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5101 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5161 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     5221 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5281 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     5341 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     5401 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     5461 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     5521 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     5581 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     5641 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     5701 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     5761 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     5821 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     5881 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     5941 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6001 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6061 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6121 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6181 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6241 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6301 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     6361 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     6421 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     6481 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     6541 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     6601 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     6661 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     6721 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     6781 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     6841 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     6901 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     6961 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7021 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7081 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7141 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7201 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7261 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7321 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     7381 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     7441 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     7501 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     7561 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     7621 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     7681 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     7741 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     7801 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     7861 AACGCGAATT TT
//