LOCUS       RC222608                6948 bp    DNA     circular  SYN 
DEFINITION  PRDM8 (Myc-DDK-tagged)-Human PR domain containing 8 (PRDM8), transcript variant 2
ACCESSION   NM_001099403
VERSION     NM_001099403.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3105..3196 
                     /label="Myc-DDK"  
     misc_feature    3226..3811 
                     /label="PolyA signal"  
     misc_feature    3283..3302 
                     /label="Restriction Enzyme"  
     misc_feature    4087..4759 
                     /label="ColE1"  
     misc_feature    5189..5983 
                     /label="Kan/Neo"  
     misc_feature    6018..6320 
                     /label="SV40 ori"  
     misc_feature    6406..6883 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3087..3093 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3087 
                     /label="PRDM8"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGATACT GGCATCCAGC GAGGCATCTG GGATGGAGAT GCCAAGGCTG 
     1081 TCCAACAATG TCTGACAGAT ATTTTTACCA GCGTTTACAC CACCTGCGAC ATCCCTGAGA 
     1141 ATGCTATATT TGGTCCCTGT GTCCTGAGCC ATACTTCCCT ATATGACAGC ATAGCTTTCA 
     1201 TAGCTCTCAA GTCTACTGAC AAGAGAACAG TACCGTATAT CTTTCGGGTA GACACCTCAG 
     1261 CAGCAAATGG TTCCTCAGAA GGTCTCATGT GGCTGCGGTT GGTCCAATCG GCCAGAGATA 
     1321 AGGAAGAGCA GAACCTTGAA GCCTACATAA AAAACGGACA GCTGTTCTAC CGCTCTCTCC 
     1381 GCAGGATTGC CAAAGACGAG GAGTTACTAG TTTGGTACGG GAAAGAACTG ACTGAGTTAC 
     1441 TCTTGCTCTG CCCCTCTAGA TCCCACAACA AAATGAATGG GTCGTCCCCT TACACATGCC 
     1501 TGGAATGCAG CCAACGTTTC CAGTTTGAGT TCCCCTATGT GGCGCATCTG CGTTTCCGCT 
     1561 GCCCCAAGAG ACTTCACAGC GCTGATATAA GTCCCCAAGA CGAACAAGGC GGCGGCGTGG 
     1621 GCACCAAGGA CCACGGGGGC GGCGGCGGCG GTGGCAAAGA CCAGCAGCAG CAGCAGCAGG 
     1681 AGGCACCTTT AGGCCCGGGT CCCAAGTTTT GCAAAGCCGG CCCCCTCCAC CACTACCCAT 
     1741 CCCCCTCCCC GGAAAGCAGC AACCCATCCG CTGCCGCCGG CGGCAGCAGC GCGAAGCCAT 
     1801 CCACAGACTT CCACAACCTG GCCAGGGAGC TGGAAAACTC CCGGGGAGGC AGCAGCTGCT 
     1861 CCCCAGCCCA GAGCCTCAGC AGCGGTAGCG GCAGCGGCGG CGGCGGCGGC CACCAGGAGG 
     1921 CGGAGCTGAG TCCCGACGGC ATCGCCACGG GCGGCGGCAA AGGAAAGAGG AAATTCCCGG 
     1981 AGGAGGCGGC GGAGGGCGGC GGTGGCGCTG GTCTGGTAGG GGGCCGGGGC CGCTTCGTAG 
     2041 AGCGGCCCCT CCCGGCCTCC AAGGAGGATC TGGTGTGCAC ACCGCAGCAG TACCGAGCCT 
     2101 CGGGCAGCTA CTTCGGCCTG GAAGAGAACG GCCGCCTCTT CGCGCCGCCA AGTCCCGAGA 
     2161 CGGGCGAGGC GAAGCGCAGC GCCTTCGTGG AGGTGAAGAA GGCTGCCCGC GCGGCCAGCC 
     2221 TGCAGGAGGA GGGGACAGCC GACGGCGCGG GAGTCGCCTC CGAGGACCAG GACGCTGGCG 
     2281 GCGGCGGCGG CTCCTCCACG CCCGCGGCCG CGTCACCGGT GGGCGCCGAG AAGCTGCTGG 
     2341 CCCCGCGGCC TGGGGGCCCG CTGCCCAGCC GGCTCGAGGG CGGCAGTCCT GCGAGGGGCA 
     2401 GCGCCTTCAC TTCGGTGCCG CAGCTGGGCA GCGCGGGCAG CACCAGCGGT GGGGGCGGAA 
     2461 CGGGCGCCGG GGCCGCAGGC GGCGCGGGCG GGGGCCAGGG CGCCGCGTCG GACGAGCGCA 
     2521 AAAGCGCCTT CTCGCAGCCA GCACGCTCTT TCTCGCAGCT GTCCCCGCTG GTGCTGGGCC 
     2581 AGAAGCTGGG CGCGCTCGAG CCATGCCACC CCGCCGACGG CGTGGGCCCC ACCAGACTCT 
     2641 ATCCCGCCGC CGCGGACCCT CTAGCGGTGA AGCTCCAGGG GGCCGCGGAC CTGAACGGAG 
     2701 GTTGCGGGTC CCTGCCGAGC GGCGGCGGCG GCCTGCCTAA GCAGAGCCCC TTCCTGTACG 
     2761 CCACCGCCTT CTGGCCCAAG AGCTCCGCTG CCGCTGCAGC CGCGGCTGCG GCGGCGGCCG 
     2821 CGGGGCCCTT GCAGCTGCAG CTGCCCTCGG CGCTCACGCT GCTGCCGCCC TCCTTCACCT 
     2881 CGCTGTGTCT GCCCGCGCAG AACTGGTGCG CCAAGTGCAA TGCCTCCTTC CGCATGACCT 
     2941 CCGACCTGGT GTACCATATG AGGTCGCACC ACAAAAAGGA GTATGCGATG GAGCCCTTGG 
     3001 TGAAGCGGCG GCGAGAGGAG AAACTCAAGT GCCCCATCTG CAATGAGTCC TTCAGGGAGC 
     3061 GCCACCACCT CTCCAGGCAC ATGACCTCGC ATAATACGCG TACGCGGCCG CTCGAGCAGA 
     3121 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     3181 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     3241 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     3301 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     3361 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     3421 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     3481 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     3541 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     3601 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     3661 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     3721 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     3781 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     3841 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     3901 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     3961 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     4021 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     4081 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     4141 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     4201 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     4261 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     4321 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     4381 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     4441 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     4501 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     4561 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     4621 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     4681 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     4741 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     4801 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     4861 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     4921 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     4981 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     5041 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     5101 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     5161 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     5221 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     5281 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     5341 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     5401 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     5461 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     5521 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     5581 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     5641 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     5701 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     5761 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     5821 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     5881 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     5941 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     6001 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     6061 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     6121 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     6181 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6241 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     6301 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     6361 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     6421 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     6481 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     6541 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     6601 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     6661 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     6721 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     6781 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     6841 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     6901 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//