LOCUS       RC222574                8718 bp    DNA     circular  SYN 
DEFINITION  ABCB4 (Myc-DDK-tagged)-Human ATP-binding cassette, sub-family B (MDR/TAP), member 4 (ABCB4), transcript variant A
ACCESSION   NM_000443
VERSION     NM_000443.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4875..4966 
                     /label="Myc-DDK"  
     misc_feature    4996..5581 
                     /label="PolyA signal"  
     misc_feature    5053..5072 
                     /label="Restriction Enzyme"  
     misc_feature    5857..6529 
                     /label="ColE1"  
     misc_feature    6959..7753 
                     /label="Kan/Neo"  
     misc_feature    7788..8090 
                     /label="SV40 ori"  
     misc_feature    8176..8653 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4857..4863 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4857 
                     /label="ABCB4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATCTTGAG GCGGCAAAGA ACGGAACAGC CTGGCGCCCC ACGAGCGCGG 
     1081 AGGGCGACTT TGAACTGGGC ATCAGCAGCA AACAAAAAAG GAAAAAAACG AAGACAGTGA 
     1141 AAATGATTGG AGTATTAACA TTGTTTCGAT ACTCCGATTG GCAGGATAAA TTGTTTATGT 
     1201 CGCTGGGTAC CATCATGGCC ATAGCTCACG GATCAGGTCT CCCCCTCATG ATGATAGTAT 
     1261 TTGGAGAGAT GACTGACAAA TTTGTTGATA CTGCAGGAAA CTTCTCCTTT CCAGTGAACT 
     1321 TTTCCTTGTC GCTGCTAAAT CCAGGCAAAA TTCTGGAAGA AGAAATGACT AGATATGCAT 
     1381 ATTACTACTC AGGATTGGGT GCTGGAGTTC TTGTTGCTGC CTATATACAA GTTTCATTTT 
     1441 GGACTTTGGC AGCTGGTCGA CAGATCAGGA AAATTAGGCA GAAGTTTTTT CATGCTATTC 
     1501 TACGACAGGA AATAGGATGG TTTGACATCA ATGACACCAC TGAACTCAAT ACGCGGCTAA 
     1561 CAGATGACAT CTCCAAAATC AGTGAAGGAA TTGGTGACAA GGTTGGAATG TTCTTTCAAG 
     1621 CAGTAGCCAC GTTTTTTGCA GGATTCATAG TGGGATTCAT CAGAGGATGG AAGCTCACCC 
     1681 TTGTGATAAT GGCCATCAGC CCTATTCTAG GACTCTCTGC AGCCGTTTGG GCAAAGATAC 
     1741 TCTCGGCATT TAGTGACAAA GAACTAGCTG CTTATGCAAA AGCAGGCGCC GTGGCAGAAG 
     1801 AGGCTCTGGG GGCCATCAGG ACTGTGATAG CTTTCGGGGG CCAGAACAAA GAGCTGGAAA 
     1861 GGTATCAGAA ACATTTAGAA AATGCCAAAG AGATTGGAAT TAAAAAAGCT ATTTCAGCAA 
     1921 ACATTTCCAT GGGTATTGCC TTCCTGTTAA TATATGCATC ATATGCACTG GCCTTCTGGT 
     1981 ATGGATCCAC TCTAGTCATA TCAAAAGAAT ATACTATTGG AAATGCAATG ACAGTTTTTT 
     2041 TTTCAATCCT AATTGGAGCT TTCAGTGTTG GCCAGGCTGC CCCATGTATT GATGCTTTTG 
     2101 CCAATGCAAG AGGAGCAGCA TATGTGATCT TTGATATTAT TGATAATAAT CCTAAAATTG 
     2161 ACAGTTTTTC AGAGAGAGGA CACAAACCAG ACAGCATCAA AGGGAATTTG GAGTTCAATG 
     2221 ATGTTCACTT TTCTTACCCT TCTCGAGCTA ACGTCAAGAT CTTGAAGGGC CTCAACCTGA 
     2281 AGGTGCAGAG TGGGCAGACG GTGGCCCTGG TTGGAAGTAG TGGCTGTGGG AAGAGCACAA 
     2341 CGGTCCAGCT GATACAGAGG CTCTATGACC CTGATGAGGG CACAATTAAC ATTGATGGGC 
     2401 AGGATATTAG GAACTTTAAT GTAAACTATC TGAGGGAAAT CATTGGTGTG GTGAGTCAGG 
     2461 AGCCGGTGCT GTTTTCCACC ACAATTGCTG AAAATATTTG TTATGGCCGT GGAAATGTAA 
     2521 CCATGGATGA GATAAAGAAA GCTGTCAAAG AGGCCAACGC CTATGAGTTT ATCATGAAAT 
     2581 TACCACAGAA ATTTGACACC CTGGTTGGAG AGAGAGGGGC CCAGCTGAGT GGTGGGCAGA 
     2641 AGCAGAGGAT CGCCATTGCA CGTGCCCTGG TTCGCAACCC CAAGATCCTT CTGCTGGATG 
     2701 AGGCCACGTC AGCATTGGAC ACAGAAAGTG AAGCTGAGGT ACAGGCAGCT CTGGATAAGG 
     2761 CCAGAGAAGG CCGGACCACC ATTGTGATAG CACACCGACT GTCTACGGTC CGAAATGCAG 
     2821 ATGTCATCGC TGGGTTTGAG GATGGAGTAA TTGTGGAGCA AGGAAGCCAC AGCGAACTGA 
     2881 TGAAGAAGGA AGGGGTGTAC TTCAAACTTG TCAACATGCA GACATCAGGA AGCCAGATCC 
     2941 AGTCAGAAGA ATTTGAACTA AATGATGAAA AGGCTGCCAC TAGAATGGCC CCAAATGGCT 
     3001 GGAAATCTCG CCTATTTAGG CATTCTACTC AGAAAAACCT TAAAAATTCA CAAATGTGTC 
     3061 AGAAGAGCCT TGATGTGGAA ACCGATGGAC TTGAAGCAAA TGTGCCACCA GTGTCCTTTC 
     3121 TGAAGGTCCT GAAACTGAAT AAAACAGAAT GGCCCTACTT TGTCGTGGGA ACAGTATGTG 
     3181 CCATTGCCAA TGGGGGGCTT CAGCCGGCAT TTTCAGTCAT ATTCTCAGAG ATCATAGCGA 
     3241 TTTTTGGACC AGGCGATGAT GCAGTGAAGC AGCAGAAGTG CAACATATTC TCTTTGATTT 
     3301 TCTTATTTCT GGGAATTATT TCTTTTTTTA CTTTCTTCCT TCAGGGTTTC ACGTTTGGGA 
     3361 AAGCTGGCGA GATCCTCACC AGAAGACTGC GGTCAATGGC TTTTAAAGCA ATGCTAAGAC 
     3421 AGGACATGAG CTGGTTTGAT GACCATAAAA ACAGTACTGG TGCACTTTCT ACAAGACTTG 
     3481 CCACAGATGC TGCCCAAGTC CAAGGAGCCA CAGGAACCAG GTTGGCTTTA ATTGCACAGA 
     3541 ATATAGCTAA CCTTGGAACT GGTATTATCA TATCATTTAT CTACGGTTGG CAGTTAACCC 
     3601 TATTGCTATT AGCAGTTGTT CCAATTATTG CTGTGTCAGG AATTGTTGAA ATGAAATTGT 
     3661 TGGCTGGAAA TGCCAAAAGA GATAAAAAAG AACTGGAAGC TGCTGGAAAG ATTGCAACAG 
     3721 AGGCAATAGA AAATATTAGG ACAGTTGTGT CTTTGACCCA GGAAAGAAAA TTTGAATCAA 
     3781 TGTATGTTGA AAAATTGTAT GGACCTTACA GGAATTCTGT GCAGAAGGCA CACATCTATG 
     3841 GAATTACTTT TAGTATCTCA CAAGCATTTA TGTATTTTTC CTATGCCGGT TGTTTTCGAT 
     3901 TTGGTGCATA TCTCATTGTG AATGGACATA TGCGCTTCAG AGATGTTATT CTGGTGTTTT 
     3961 CTGCAATTGT ATTTGGTGCA GTGGCTCTAG GACATGCCAG TTCATTTGCT CCAGACTATG 
     4021 CTAAAGCTAA GCTGTCTGCA GCCCACTTAT TCATGCTGTT TGAAAGACAA CCTCTGATTG 
     4081 ACAGCTACAG TGAAGAGGGG CTGAAGCCTG ATAAATTTGA AGGAAATATA ACATTTAATG 
     4141 AAGTCGTGTT CAACTATCCC ACCCGAGCAA ACGTGCCAGT GCTTCAGGGG CTGAGCCTGG 
     4201 AGGTGAAGAA AGGCCAGACA CTAGCCCTGG TGGGCAGCAG TGGCTGTGGG AAGAGCACGG 
     4261 TGGTCCAGCT CCTGGAGCGG TTCTACGACC CCTTGGCGGG GACAGTGCTT CTCGATGGTC 
     4321 AAGAAGCAAA GAAACTCAAT GTCCAGTGGC TCAGAGCTCA ACTCGGAATC GTGTCTCAGG 
     4381 AGCCTATCCT ATTTGACTGC AGCATTGCCG AGAATATTGC CTATGGAGAC AACAGCCGGG 
     4441 TTGTATCACA GGATGAAATT GTGAGTGCAG CCAAAGCTGC CAACATACAT CCTTTCATCG 
     4501 AGACGTTACC CCACAAATAT GAAACAAGAG TGGGAGATAA GGGGACTCAG CTCTCAGGAG 
     4561 GTCAAAAACA GAGGATTGCT ATTGCCCGAG CCCTCATCAG ACAACCTCAA ATCCTCCTGT 
     4621 TGGATGAAGC TACATCAGCT CTGGATACTG AAAGTGAAAA GGTTGTCCAA GAAGCCCTGG 
     4681 ACAAAGCCAG AGAAGGCCGC ACCTGCATTG TGATTGCTCA CCGCCTGTCC ACCATCCAGA 
     4741 ATGCAGACTT AATAGTGGTG TTTCAGAATG GGAGAGTCAA GGAGCATGGC ACGCATCAGC 
     4801 AGCTGCTGGC ACAGAAAGGC ATCTATTTTT CAATGGTCAG TGTCCAGGCT GGGACACAGA 
     4861 ACTTAACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4921 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4981 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     5041 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     5101 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     5161 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5221 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5281 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5341 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5401 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5461 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5521 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5581 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5641 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5701 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5761 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5821 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5881 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5941 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     6001 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     6061 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     6121 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6181 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6241 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6301 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6361 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6421 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6481 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6541 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6601 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6661 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6721 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6781 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6841 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6901 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6961 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     7021 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     7081 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     7141 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7201 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7261 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7321 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7381 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7441 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7501 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7561 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7621 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7681 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7741 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7801 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7861 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7921 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7981 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8041 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8101 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     8161 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8221 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8281 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8341 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8401 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8461 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8521 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8581 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8641 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8701 AAATTTAACG CGAATTTT
//