LOCUS       RC222562                8655 bp    DNA     circular  SYN 
DEFINITION  STAG1 (Myc-DDK-tagged)-Human stromal antigen 1 (STAG1)
ACCESSION   NM_005862
VERSION     NM_005862.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4812..4903 
                     /label="Myc-DDK"  
     misc_feature    4933..5518 
                     /label="PolyA signal"  
     misc_feature    4990..5009 
                     /label="Restriction Enzyme"  
     misc_feature    5794..6466 
                     /label="ColE1"  
     misc_feature    6896..7690 
                     /label="Kan/Neo"  
     misc_feature    7725..8027 
                     /label="SV40 ori"  
     misc_feature    8113..8590 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4794..4800 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4794 
                     /label="STAG1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATTACTTCA GAATTACCAG TGTTACAGGA TTCAACTAAT GAAACTACTG 
     1081 CCCATTCCGA TGCTGGCAGC GAGCTTGAAG AAACAGAGGT CAAAGGAAAA AGAAAAAGGG 
     1141 GTCGTCCTGG CCGGCCTCCA TCTACAAATA AGAAACCTCG AAAATCTCCA GGTGAGAAGA 
     1201 GCAGAATTGA AGCTGGAATT AGAGGAGCAG GCCGTGGAAG AGCTAATGGA CACCCTCAAC 
     1261 AGAATGGGGA AGGGGAGCCT GTCACATTAT TTGAGGTGGT GAAACTGGGG AAAAGTGCAA 
     1321 TGCAGTCCGT GGTGGATGAC TGGATTGAAT CATATAAACA AGACAGGGAC ATCGCACTTC 
     1381 TGGATTTAAT CAACTTTTTT ATCCAGTGTT CAGGATGTCG AGGTACTGTG AGAATAGAGA 
     1441 TGTTTCGAAA TATGCAGAAT GCAGAAATCA TCAGAAAAAT GACTGAAGAA TTTGATGAGG 
     1501 ACAGTGGTGA TTATCCTCTT ACCATGCCTG GACCTCAGTG GAAAAAATTT CGTTCAAACT 
     1561 TTTGTGAATT TATTGGAGTC CTGATTCGAC AGTGTCAGTA TAGCATAATT TATGATGAGT 
     1621 ATATGATGGA CACAGTAATC TCCCTTTTGA CGGGTTTGTC AGACTCCCAG GTCAGAGCTT 
     1681 TTAGGCATAC AAGTACCCTG GCTGCCATGA AGCTCATGAC TGCTCTGGTG AATGTTGCCT 
     1741 TAAACCTCAG TATTCATCAG GATAATACCC AGAGACAATA TGAAGCCGAG AGAAATAAAA 
     1801 TGATTGGGAA GAGAGCCAAT GAAAGGTTGG AGTTACTACT TCAGAAACGC AAAGAGCTGC 
     1861 AAGAAAATCA GGATGAAATC GAAAATATGA TGAACTCTAT TTTTAAGGGT ATATTTGTTC 
     1921 ATAGATACCG TGATGCTATT GCTGAGATTA GAGCCATTTG TATTGAAGAA ATTGGAGTAT 
     1981 GGATGAAAAT GTATAGTGAT GCCTTCCTAA ATGACAGTTA CCTAAAATAT GTTGGCTGGA 
     2041 CTCTTCATGA CAGGCAAGGG GAAGTCAGGC TGAAGTGTTT GAAAGCTCTG CAGAGTCTAT 
     2101 ATACCAATAG AGAATTATTC CCCAAATTGG AACTATTCAC TAACCGATTC AAGGATCGCA 
     2161 TTGTATCAAT GACACTTGAT AAAGAATATG ATGTTGCTGT GGAAGCTATT CGATTGGTTA 
     2221 CTCTGATACT TCATGGAAGT GAAGAAGCTC TTTCCAATGA AGACTGTGAA AATGTTTACC 
     2281 ACTTGGTGTA CTCGGCACAT CGCCCTGTTG CTGTGGCAGC TGGAGAGTTC CTTCACAAAA 
     2341 AGCTATTTAG CAGACATGAC CCACAAGCAG AAGAAGCATT AGCAAAGAGG AGGGGAAGAA 
     2401 ACAGCCCGAA TGGAAACCTC ATTAGGATGC TGGTTCTTTT CTTTCTTGAA AGTGAGTTAC 
     2461 ATGAACATGC AGCCTACTTG GTGGACAGTT TATGGGAGAG CTCTCAAGAA CTGTTGAAAG 
     2521 ACTGGGAATG TATGACAGAG TTGCTATTAG AAGAACCTGT TCAAGGAGAG GAAGCAATGT 
     2581 CTGATCGTCA AGAGAGTGCT CTTATAGAGC TAATGGTTTG TACAATTCGT CAAGCTGCTG 
     2641 AGGCACATCC TCCAGTGGGA AGGGGTACCG GCAAGAGAGT GCTAACTGCC AAAGAAAGGA 
     2701 AAACTCAAAT TGATGATAGA AACAAATTGA CTGAACATTT TATTATTACA CTTCCTATGT 
     2761 TACTGTCAAA GTATTCTGCA GATGCAGAGA AGGTAGCAAA CTTGCTACAA ATCCCACAGT 
     2821 ATTTTGATTT AGAAATCTAC AGCACAGGTA GAATGGAAAA GCATCTGGAT GCTTTATTAA 
     2881 AACAGATTAA GTTTGTTGTG GAGAAACACG TAGAATCAGA TGTTCTAGAA GCCTGCAGTA 
     2941 AAACCTATAG TATCTTATGC AGTGAAGAAT ATACCATCCA GAACAGAGTT GACATAGCTC 
     3001 GAAGCCAGCT GATTGATGAG TTTGTAGATC GATTCAATCA TTCTGTGGAA GACCTATTGC 
     3061 AAGAGGGAGA AGAAGCTGAT GATGATGACA TTTACAATGT TCTTTCTACA TTAAAGCGGT 
     3121 TAACTTCTTT TCACAATGCA CATGATCTCA CAAAATGGGA TCTCTTTGGT AATTGCTACA 
     3181 GATTATTGAA GACTGGAATT GAACATGGAG CCATGCCAGA ACAGATAGTC GTGCAAGCAC 
     3241 TGCAGTGTTC CCATTATTCG ATTCTTTGGC AGTTGGTGAA AATTACTGAT GGCTCTCCTT 
     3301 CCAAAGAGGA TTTGTTGGTA TTGAGGAAAA CGGTGAAATC CTTTTTGGCT GTTTGCCAGC 
     3361 AGTGCCTGTC TAATGTTAAT ACTCCAGTGA AAGAACAGGC TTTCATGTTA CTCTGTGATC 
     3421 TTCTGATGAT TTTCAGCCAC CAATTAATGA CAGGTGGCAG AGAGGGCCTT CAGCCTTTGG 
     3481 TGTTCAATCC AGATACTGGA CTCCAATCTG AACTCCTCAG TTTTGTGATG GATCACGTTT 
     3541 TTATTGACCA AGACGAGGAG AACCAGAGCA TGGAGGGTGA TGAAGAAGAT GAAGCTAATA 
     3601 AAATTGAGGC CTTACATAAA AGAAGGAATC TACTTGCTGC TTTCAGCAAA CTTATCATTT 
     3661 ATGACATTGT TGACATGCAT GCAGCTGCAG ACATCTTCAA ACACTACATG AAGTATTACA 
     3721 ATGACTATGG TGATATTATT AAGGAAACAC TGAGTAAAAC CAGGCAGATT GATAAAATTC 
     3781 AGTGTGCCAA GACTCTCATT CTCAGTTTGC AACAGTTATT TAATGAACTT GTTCAAGAGC 
     3841 AAGGTCCCAA CCTAGATAGG ACATCTGCCC ATGTCAGTGG CATTAAAGAA CTGGCACGTC 
     3901 GCTTTGCCCT TACATTTGGA TTGGACCAGA TTAAGACACG AGAAGCAGTT GCCACACTTC 
     3961 ACAAGGATGG CATAGAGTTT GCATTTAAAT ACCAAAATCA GAAAGGACAA GAGTATCCAC 
     4021 CTCCTAATCT GGCTTTTCTT GAAGTACTAA GTGAATTTTC TTCTAAACTT CTTCGACAGG 
     4081 ACAAAAAGAC AGTTCATTCA TACCTAGAGA AATTCCTTAC CGAGCAGATG ATGGAAAGGA 
     4141 GGGAGGATGT ATGGCTTCCA CTCATCTCCT ATAGAAATTC ATTAGTCACT GGGGGTGAAG 
     4201 ATGATAGAAT GTCTGTGAAC AGTGGAAGTA GCAGCAGCAA AACCTCATCA GTAAGGAATA 
     4261 AGAAAGGACG ACCTCCACTT CATAAAAAAC GAGTAGAAGA TGAGAGTCTG GATAACACAT 
     4321 GGCTAAACAG GACTGACACC ATGATTCAGA CTCCTGGCCC CCTGCCAGCA CCACAACTCA 
     4381 CATCCACTGT ACTGCGGGAG AACAGTCGGC CCATGGGAGA CCAGATTCAA GAACCTGAGT 
     4441 CTGAACATGG TTCTGAACCA GACTTTTTAC ACAATCCTCA GATGCAGATC TCTTGGTTAG 
     4501 GCCAGCCGAA GTTAGAAGAC TTAAATCGGA AGGACAGAAC AGGAATGAAC TACATGAAAG 
     4561 TGAGAACTGG AGTGAGGCAT GCTGTTCGGG GTCTAATGGA GGAAGATGCT GAGCCCATCT 
     4621 TTGAAGATGT GATGATGTCA TCCCGAAGCC AGTTAGAAGA TATGAATGAA GAATTTGAGG 
     4681 ACACCATGGT TATTGATCTG CCTCCATCAA GAAATCGGCG AGAGAGAGCT GAGCTAAGGC 
     4741 CAGACTTCTT TGACTCTGCA GCTATCATAG AAGATGATTC AGGATTTGGA ATGCCTATGT 
     4801 TCACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4861 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4921 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4981 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     5041 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     5101 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     5161 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5221 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5281 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5341 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5401 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5461 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5521 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5581 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5641 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5701 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5761 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5821 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5881 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5941 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     6001 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     6061 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     6121 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6181 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6241 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6301 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6361 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6421 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6481 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6541 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6601 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6661 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6721 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6781 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6841 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6901 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6961 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     7021 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     7081 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     7141 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7201 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7261 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7321 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7381 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7441 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7501 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7561 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7621 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7681 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7741 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7801 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7861 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7921 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7981 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8041 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     8101 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     8161 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8221 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8281 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8341 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8401 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8461 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8521 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8581 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8641 TTTAACGCGA ATTTT
//