LOCUS       RC222530L1                9387 bp    DNA     circular  SYN 
DEFINITION  PHC1 (Myc-DDK-tagged)-Human polyhomeotic homolog 1 (Drosophila) (PHC1)
ACCESSION   NM_004426
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7678..7858 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8586..9245 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    8017..8585 
                     /label="ColE1"  
     misc_feature    6553..6573 
                     /label="LR50 primer"  
     misc_feature    6350..6421 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6562..7153 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6332..6338 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6332 
                     /label="PHC1"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGAGACTGAG AGCGAGCAGA ACTCCAATTC 
     3361 CACCAATGGG AGTTCTAGCT CAGGGGGCAG CTCTCGGCCC CAGATAGCTC AAATGTCACT 
     3421 ATATGAACGA CAAGCAGTGC AGGCTCTGCA AGCACTGCAG CGGCAGCCCA ATGCAGCTCA 
     3481 GTATTTCCAC CAGTTCATGC TCCAGCAGCA GCTCAGTAAT GCCCAGCTGC ATAGCCTGGC 
     3541 TGCCGTCCAG CAGGCCACAA TTGCTGCCAG TCGGCAGGCC AGCTCCCCAA ACACCAGCAC 
     3601 TACACAGCAG CAGACTACCA CCACCCAGGC CTCGATCAAT CTGGCCACCA CATCGGCCGC 
     3661 CCAGCTCATC AGCCGATCCC AGAGTGTGAG CTCTCCCAGT GCTACCACCT TGACCCAATC 
     3721 TGTGCTACTG GGGAACACCA CCTCCCCACC CCTCAACCAG TCTCAGGCCC AGATGTATCT 
     3781 ACGGCCACAG CTGGGAAACC TATTGCAGGT AAACCGAACC CTGGGTCGGA ATGTGCCTCT 
     3841 AGCCTCCCAA CTCATCCTGA TGCCTAATGG GGCGGTGGCT GCAGTCCAGC AGGAGGTGCC 
     3901 ATCTGCTCAG TCTCCTGGAG TTCATGCAGA TGCAGATCAG GTTCAGAACT TGGCAGTAAG 
     3961 GAATCAACAG GCCTCAGCTC AAGGACCTCA GATGCAAGGC TCCACTCAGA AGGCCATTCC 
     4021 TCCAGGAGCC TCCCCTGTCT CTAGCCTCTC CCAGGCCTCT AGCCAGGCCC TAGCGGTGGC 
     4081 ACAGGCTTCC TCTGGGGCCA CAAACCAGTC CCTCAACCTT AGTCAAGCTG GTGGAGGCAG 
     4141 TGGGAATAGC ATCCCAGGGT CCATGGGTCC AGGTGGAGGT GGGCAGGCAC ATGGTGGTTT 
     4201 GGGTCAGTTG CCTTCCTCAG GAATGGGTGG TGGGAGCTGT CCCAGGAAGG GTACAGGAGT 
     4261 GGTGCAGCCC TTGCCTGCAG CCCAAACAGT GACTGTGAGC CAGGGCAGCC AGACAGAGGC 
     4321 AGAAAGTGCA GCAGCCAAGA AGGCAGAAGC AGATGGGAGT GGCCAGCAGA ATGTGGGCAT 
     4381 GAACCTGACA CGGACAGCCA CACCTGCGCC CAGCCAGACA CTTATTAGCT CAGCCACCTA 
     4441 CACACAGATC CAGCCCCATT CACTGATTCA GCAACAGCAA CAGATCCACC TCCAGCAGAA 
     4501 ACAGGTGGTG ATCCAGCAGC AGATTGCCAT CCACCACCAG CAGCAGTTCC AGCACCGGCA 
     4561 GTCCCAGCTC CTTCACACAG CTACACACCT CCAGTTGGCG CAGCAGCAGC AGCAGCAACA 
     4621 ACAGCAACAG CAGCAACAGC AGCAGCCGCA AGCCACCACC CTCACTGCCC CTCAGCCACC 
     4681 ACAGGTCCCA CCTACTCAGC AGGTCCCACC TTCCCAGTCC CAGCAGCAAG CCCAAACCCT 
     4741 GGTCGTTCAG CCCATGCTTC AGTCTTCACC CTTGTCTCTT CCACCTGATG CAGCCCCTAA 
     4801 GCCACCAATT CCCATCCAAT CCAAACCACC TGTAGCACCT ATCAAGCCGC CTCAGTTAGG 
     4861 GGCCGCTAAG ATGTCAGCTG CCCAGCAACC ACCACCCCAT ATCCCTGTGC AAGTTGTAGG 
     4921 CACTCGACAG CCAGGTACAG CCCAGGCACA GGCTTTGGGG TTGGCACAGC TGGCAGCTGC 
     4981 TGTACCTACT TCCCGGGGGA TGCCAGGTAC AGTGCAGTCT GGTCAGGCCC ATTTGGCCTC 
     5041 CTCGCCACCT TCATCCCAGG CTCCTGGTGC ACTGCAGGAG TGCCCTCCCA CATTGGCCCC 
     5101 TGGGATGACC CTTGCTCCTG TGCAGGGGAC AGCACATGTG GTAAAGGGTG GGGCTACCAC 
     5161 CTCCTCACCT GTTGTAGCCC AGGTCCCTGC TGCCTTCTAT ATGCAGTCTG TGCACTTGCC 
     5221 GGGTAAACCC CAGACATTGG CTGTCAAACG CAAGGCTGAC TCTGAGGAGG AGAGAGATGA 
     5281 TGTCTCCACA TTGGGTTCAA TGCTTCCTGC CAAGGCATCT CCAGTAGCAG AAAGCCCAAA 
     5341 AGTCATGGAC GAGAAGAGCA GTCTTGGAGA AAAAGCTGAA TCAGTGGCTA ATGTGAATGC 
     5401 TAATACTCCA AGCAGTGAAC TAGTAGCCTT GACCCCCGCC CCTTCAGTAC CGCCTCCTAC 
     5461 ACTAGCCATG GTGTCTAGAC AAATGGGTGA CTCAAAACCC CCACAGGCCA TCGTGAAGCC 
     5521 CCAGATTCTC ACCCACATCA TTGAAGGCTT TGTTATCCAG GAAGGAGCAG AACCTTTCCC 
     5581 GGTGGGTTGT TCTCAGTTAC TGAAGGAGTC TGAGAAGCCA CTACAGACTG GCCTTCCGAC 
     5641 AGGGCTGACT GAGAATCAGT CAGGTGGCCC TTTGGGAGTG GACAGCCCAT CTGCTGAGTT 
     5701 AGATAAGAAG GCGAATCTCC TGAAGTGCGA GTACTGTGGG AAGTACGCCC CCGCAGAGCA 
     5761 GTTTCGTGGC TCTAAGAGGT TCTGCTCCAT GACTTGCGCT AAGAGGTACA ATGTGAGCTG 
     5821 TAGCCATCAG TTCCGGCTGA AGAGGAAAAA AATGAAAGAG TTTCAAGAAG CCAACTATGC 
     5881 TCGCGTTCGC AGGCGTGGAC CCCGCCGCAG CTCCTCTGAC ATTGCCCGTG CCAAGATTCA 
     5941 GGGCAAGTGC CACCGGGGTC AAGAAGACTC TAGCCGGGGT TCAGATAATT CCAGTTATGA 
     6001 TGAAGCACTC TCTCCAACAT CTCCTGGGCC TTTATCAGTA AGAGCTGGGC ATGGAGAACG 
     6061 TGACCTGGGG AATCCCAATA CAGCTCCACC TACACCGGAA TTACATGGCA TCAACCCTGT 
     6121 GTTCCTGTCC AGTAATCCCA GCCGTTGGAG TGTAGAGGAG GTGTACGAGT TTATTGCTTC 
     6181 TCTCCAAGGC TGCCAAGAGA TTGCAGAGGA ATTTCGCTCA CAGGAGATTG ATGGACAGGC 
     6241 CCTTTTATTA CTTAAAGAAG AACATCTTAT GAGTGCCATG AACATCAAGC TGGGCCCTGC 
     6301 CCTCAAGATC TGCGCCAAGA TAAATGTCCT CAAGGAGACC ACGCGTACGC GGCCGCTCGA 
     6361 GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA 
     6421 CGACGATAAG GTTTAAACGG CCGGCCGCGG TCTGTACAAG TAGGATTCGT CGAGGGACCT 
     6481 AATAACTTCG TATAGCATAC ATTATACGAA GTTATACATG TTTAAGGGTT CCGGTTCCAC 
     6541 TAGGTACAAT TCGATATCAA GCTTATCGAT AATCAACCTC TGGATTACAA AATTTGTGAA 
     6601 AGATTGACTG GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA 
     6661 ATGCCTTTGT ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA 
     6721 TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGTGGTG 
     6781 TGCACTGTGT TTGCTGACGC AACCCCCACT GGTTGGGGCA TTGCCACCAC CTGTCAGCTC 
     6841 CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT ATTGCCACGG CGGAACTCAT CGCCGCCTGC 
     6901 CTTGCCCGCT GCTGGACAGG GGCTCGGCTG TTGGGCACTG ACAATTCCGT GGTGTTGTCG 
     6961 GGGAAATCAT CGTCCTTTCC TTGGCTGCTC GCCTGTGTTG CCACCTGGAT TCTGCGCGGG 
     7021 ACGTCCTTCT GCTACGTCCC TTCGGCCCTC AATCCAGCGG ACCTTCCTTC CCGCGGCCTG 
     7081 CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT CGCCTTCGCC CTCAGACGAG TCGGATCTCC 
     7141 CTTTGGGCCG CCTCCCCGCA TCGATACCGT CGACCTCGAT CGAGACCTAG AAAAACATGG 
     7201 AGCAATCACA AGTAGCAATA CAGCAGCTAC CAATGCTGAT TGTGCCTGGC TAGAAGCACA 
     7261 AGAGGAGGAG GAGGTGGGTT TTCCAGTCAC ACCTCAGGTA CCTTTAAGAC CAATGACTTA 
     7321 CAAGGCAGCT GTAGATCTTA GCCACTTTTT AAAAGAAAAG GGGGGACTGG AAGGGCTAAT 
     7381 TCACTCCCAA CGAAGACAAG ATATCCTTGA TCTGTGGATC TACCACACAC AAGGCTACTT 
     7441 CCCTGATTGG CAGAACTACA CACCAGGGCC AGGGATCAGA TATCCACTGA CCTTTGGATG 
     7501 GTGCTACAAG CTAGTACCAG TTGAGCAAGA GAAGGTAGAA GAAGCCAATG AAGGAGAGAA 
     7561 CACCCGCTTG TTACACCCTG TGAGCCTGCA TGGGATGGAT GACCCGGAGA GAGAAGTATT 
     7621 AGAGTGGAGG TTTGACAGCC GCCTAGCATT TCATCACATG GCCCGAGAGC TGCATCCGGA 
     7681 CTGTACTGGG TCTCTCTGGT TAGACCAGAT CTGAGCCTGG GAGCTCTCTG GCTAACTAGG 
     7741 GAACCCACTG CTTAAGCCTC AATAAAGCTT GCCTTGAGTG CTTCAAGTAG TGTGTGCCCG 
     7801 TCTGTTGTGT GACTCTGGTA ACTAGAGATC CCTCAGACCC TTTTAGTCAG TGTGGAAAAT 
     7861 CTCTAGCAGC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     7921 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     7981 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     8041 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     8101 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     8161 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     8221 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     8281 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     8341 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     8401 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     8461 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     8521 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     8581 GATTTTGGTC ATGATTACGC CCCGCCCTGC CACTCATCGC AGTACTGTTG TAATTCATTA 
     8641 AGCATTCTGC CGACATGGAA GCCATCACAA ACGGCATGAT GAACCTGAAT CGCCAGCGGC 
     8701 ATCAGCACCT TGTCGCCTTG CGTATAATAT TTGCCCATGG TGAAAACGGG GGCGAAGAAG 
     8761 TTGTCCATAT TGGCCACGTT TAAATCAAAA CTGGTGAAAC TCACCCAGGG ATTGGCTGAG 
     8821 ACGAAAAACA TATTCTCAAT AAACCCTTTA GGGAAATAGG CCAGGTTTTC ACCGTAACAC 
     8881 GCCACATCTT GCGAATATAT GTGTAGAAAC TGCCGGAAAT CGTCGTGGTA TTCACTCCAG 
     8941 AGCGATGAAA ACGTTTCAGT TTGCTCATGG AAAACGGTGT AACAAGGGTG AACACTATCC 
     9001 CATATCACCA GCTCACCGTC TTTCATTGCC ATACGGAACT CCGGATGAGC ATTCATCAGG 
     9061 CGGGCAAGAA TGTGAATAAA GGCCGGATAA AACTTGTGCT TATTTTTCTT TACGGTCTTT 
     9121 AAAAAGGCCG TAATATCCAG CTGAACGGTC TGGTTATAGG TACATTGAGC AACTGACTGA 
     9181 AATGCCTCAA AATGTTCTTT ACGATGCCAT TGGGATATAT CAACGGTGGT ATATCCAGTG 
     9241 ATTTTTTTCT CCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     9301 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTCCCGCGC 
     9361 ACATTTCCCC GAAAAGTGCC ACCTGAC
//