LOCUS       RC222514                8697 bp    DNA     circular  SYN 
DEFINITION  SYTL2 (Myc-DDK-tagged)-Human synaptotagmin-like 2 (SYTL2), transcript variant c
ACCESSION   NM_206927
VERSION     NM_206927.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4854..4945 
                     /label="Myc-DDK"  
     misc_feature    4975..5560 
                     /label="PolyA signal"  
     misc_feature    5032..5051 
                     /label="Restriction Enzyme"  
     misc_feature    5836..6508 
                     /label="ColE1"  
     misc_feature    6938..7732 
                     /label="Kan/Neo"  
     misc_feature    7767..8069 
                     /label="SV40 ori"  
     misc_feature    8155..8632 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4836..4842 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4836 
                     /label="SYTL2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATGAACCC AATGCAGAAC AGGTCTATAA TCCCTCTCAG TTTGAGAATT 
     1081 TGAGAAAGTT TTGGGACTTA GAAGCTAATT CAAACAGTAA GGATAATGAC AAGAATATTA 
     1141 CCACCACAAG CCAAAAAAAT TCTGCACCTT TTAATAGGCA GAAACACAAG GAATTCAGCG 
     1201 ACATTAAATT ATCAGGTAAA AATACCCATG AAGCAGAGGT GCTTCTAAGC CCAAAAAAAG 
     1261 TTATGGCAAG AGAGGAAATG GAGAAATTAA ATTCAAAGGG CATACTCCAG GTGCTACCAG 
     1321 ATGAAATCAC ATTTCCTTTG AGTCCACTTA GAAAGTATAC TTATCAGTTG CCAGGAAATG 
     1381 AGTCATCAAA GGAAAATGTG GAAAAGAATA CGGAAGGGAT TGTTACTCCA GTGTTTAAGG 
     1441 AAGAAAAGGA TTACTCAGAA CAAGAGATTC AAGAATCCAT AATAAAAACC AATGTTTTGT 
     1501 CTAAAGACTG CAAAGACACT TTTAATGACA GCTTGCAGAA ACTGCTTTCA GAAACCTCAA 
     1561 CACCAGCAAT TCAACCCTCT GGTGGAAAAG TTCATGGAAA ACAAGTGCTT GAACCAAGTG 
     1621 TTTCTGAAAA TAGGACATGG CCTCAAAAAA CAGATTTTGC TGATACTGAG GAAGAAGTCA 
     1681 AAGGACCTGA GAAGATCATT AATGAGCATG TTGACAAAAC AGTAGTTCAT CCAAAGGTTA 
     1741 AACGGAACTC TTTGACTGCT AGTCTAGACA AACTCCTGAA GGAAGCAACT GGAACTTCAC 
     1801 CCTCTCCCTT GCAAGCCAAG TTGGCGCCTG TTATCACTGG AACCAACTCT AAGCTGGAAG 
     1861 AGGGGAGATT TTTTGGAAAA GGGATAGAAC AGAGTCACAA TACTTCAGCT GATAAGAGAG 
     1921 AAATACTAGC TCCTTTTCCA GTGAGAGATG AAACTTTTGG AAATACAGCT CTCCTCAAGA 
     1981 AAGCTGAAAG TGGTGAGTGC CAGCTAAGCA CACAGAATTT GATTCAGATG GCTGCAGAAG 
     2041 ATTCTCATCC ATTGGATCCA ACTTCCCAGC TTTCCAGAAA GGGTTCTTTT GGGGATGTGG 
     2101 CCAGCCCTCC CCAAGATATG CTTTTTCCCC AGGATGCTCA TCTTGTTCCC CAGGCTAGGG 
     2161 TACACCCTTC TCAAACGGAA ATTTCGGAGA CTGTAGAGAA AGTCATTCTT CCACCCAGAC 
     2221 CTGTATTGAA TGATGTAAGT GCTGCATTAC AGAAGCTGTG TGGAGAAGTA TGGTTAAGTT 
     2281 ATCCAGCTGG AAGGGAAGTA GGTCCTGGAG AAGTGAACCC AGAATTTCCT GAAGCAGTAC 
     2341 AGCCAGTATG TAGCCCCCTA AATCCTCCAG GAGTGATATC ACCATGGGCT ACGATGGACA 
     2401 CCATAGTTCC AGACAGGAAG GATTTTTATT CCTCCAATGT AGTTCCTGAT AAAACTCATG 
     2461 AAGTTGGATC TTATTTAGCT GCCCAAATGT CTCCATCAGA CCAGACGCTT AGCTCATTTG 
     2521 CTTCCATTGT TGCTCAATAT GGCAAAGGCC TCCCTCAGGA AGTGGAAGAA ATTGTGAGGG 
     2581 AAACAATTGT TCAACCCAAA TCAGAGTTCC TCGAATTCAG TGCTGGCTTA GAAAAACTAC 
     2641 TGAAGGAAGA AACTGAAACC TTCCCCTCAA AATATGAAAG TGATACAGGG AATCTTTCTC 
     2701 CATCAAAGTT AATAGGTAGT ACAGAGGAGC CCAGGCGAGC CACTTCTGAA TGCCATCCTG 
     2761 AGGAATTAAA AGAAACAGTA GAAAAGGCCG AGGCTCCGTT AATAACTGAG AGTGCTTTTG 
     2821 ATGCTGGTTT TGAGAAACTT CTTAAAGAAA TAACTGAAGC TCCTCCTTAT CAGCCCCAGG 
     2881 TGTCAGTGAG AGAAGAAACT CACGAGAAGG AGTCCTCACA GTCAGAGCAG ACCAGGTTCT 
     2941 TGGGGACAGT GCCCCATTTT TACAGGGCAG CCTCACAGAC CTCTGAAATG AAGGATAAAA 
     3001 GTAATGGTTT GGAATCTCAA GTCAACCAAT GTGATAAAAT GTTGGGAGGA GACGCACTTG 
     3061 TGACTGATTT ATTGGTAGAT TTTTGTGGTT CCAGAAGTGG AGTTGAGATC CCTAGAACCC 
     3121 CACAACTTTA TGTGGCTCAT GAAATAGGGA CCATTAAAAC TGTAACCCCC CCAGAGGACA 
     3181 GGGACAGTGA AAGTGGGGTT GCAGGGGGAC AAGGGACTCT TCAGGAACCT GGCTTTGGAG 
     3241 AGGCTTCTGA AGCAATTAGT GTGTCCAGAA ATAGGCAACC CATTCCTCTC CTGATGAACA 
     3301 AAGAAAACTC TACAAAAACA AGTAAAGTTG AATTGACTCT AGCATCGCCA TATATGAAAC 
     3361 AAGAGAAAGA GGAAGAAAAA GAAGGTTTCT CTGAGTCTGA TTTTTCAGAT GGAAACACCA 
     3421 GTTCTAATGC AGAGAGCTGG AGAAATCCTT CCAGTTCAGA AGAAGAACCC AGTCCTGTTT 
     3481 TGAAAACTTT GGAAAGGAGT GCCGCTAGGA AAATGCCTTC CAAAAGTCTA GAAGACATTT 
     3541 CATCAGATTC ATCAAATCAA GCAAAAGTAG ATAATCAGCC AGAAGAATTA GTGCGTAGTG 
     3601 CTGAAGATGA TGAGAAACCA GATCAGAAGC CAGTTACAAA TGAATGCGTA CCAAGAATTT 
     3661 CCACAGTGCC TACACAACCT GATAATCCAT TTTCTCACCC TGACAAACTC AAAAGGATGA 
     3721 GCAAGTCTGT TCCAGCATTT CTCCAAGATG AGAGTGATGA CAGAGAAACA GATACAGCAT 
     3781 CAGAAAGCAG TTACCAGCTC AGCAGACACA AGAAGAGCCC GAGCTCTTTA ACCAATCTTA 
     3841 GCAGCTCCTC TGGCATGACG TCCTTGTCTT CTGTGAGTGG CAGTGTGATG AGTGTTTATA 
     3901 GTGGAGACTT TGGCAATCTG GAAGTTAAAG GAAATATTCA GTTTGCAATT GAATATGTGG 
     3961 AGTCACTGAA GGAGTTGCAT GTTTTTGTGG CCCAGTGTAA GGACTTAGCA GCAGCGGATG 
     4021 TAAAAAAACA GCGTTCAGAC CCATATGTAA AGGCCTATTT GCTACCAGAC AAAGGCAAAA 
     4081 TGGGCAAGAA GAAAACACTC GTAGTGAAGA AAACCTTGAA TCCTGTGTAT AACGAAATAC 
     4141 TGCGGTATAA AATTGAAAAA CAAATCTTAA AGACACAGAA ATTGAACCTG TCCATTTGGC 
     4201 ATCGGGATAC ATTTAAGCGC AATAGTTTCC TAGGGGAGGT GGAACTTGAT TTGGAAACAT 
     4261 GGGACTGGGA TAACAAACAG AATAAACAAT TGAGATGGTA CCCTCTGAAG CGGAAGACAG 
     4321 CACCAGTTGC CCTTGAAGCA GAAAACAGAG GTGAAATGAA ACTAGCTCTC CAGTATGTCC 
     4381 CAGAGCCAGT CCCTGGTAAA AAGCTTCCTA CAACTGGAGA AGTGCACATC TGGGTGAAGG 
     4441 AATGCCTTGA TCTACCACTG CTAAGGGGAA GTCATCTAAA TTCTTTTGTT AAATGTACCA 
     4501 TCCTTCCAGA TACAAGTAGG AAAAGTCGCC AGAAGACAAG AGCTGTAGGG AAAACCACCA 
     4561 ACCCTATCTT CAACCACACT ATGGTGTATG ATGGGTTCAG GCCTGAAGAT CTGATGGAAG 
     4621 CCTGTGTAGA GCTTACTGTC TGGGACCATT ACAAATTAAC CAACCAATTT TTGGGAGGTC 
     4681 TTCGTATTGG CTTTGGAACA GGTAAAAGTT ATGGGACTGA AGTGGACTGG ATGGACTCTA 
     4741 CTTCAGAGGA AGTTGCTCTC TGGGAGAAGA TGGTAAACTC CCCCAATACT TGGATTGAAG 
     4801 CAACACTGCC TCTCAGAATG CTTTTGATTG CCAAGATTTC CAAAACGCGT ACGCGGCCGC 
     4861 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4921 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4981 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     5041 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     5101 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     5161 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     5221 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5281 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5341 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5401 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5461 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5521 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5581 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5641 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5701 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5761 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5821 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5881 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5941 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     6001 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     6061 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     6121 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6181 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6241 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6301 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6361 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6421 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6481 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6541 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6601 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6661 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6721 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6781 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6841 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6901 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6961 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     7021 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     7081 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     7141 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7201 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7261 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7321 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7381 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7441 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7501 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7561 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7621 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7681 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7741 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7801 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7861 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7921 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7981 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     8041 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     8101 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     8161 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8221 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8281 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8341 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8401 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8461 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8521 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8581 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8641 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//