LOCUS       RC222500                7548 bp    DNA     circular  SYN 
DEFINITION  TPO (Myc-DDK-tagged)-Human thyroid peroxidase (TPO), transcript variant 4
ACCESSION   NM_175721
VERSION     NM_175721.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3706..3797 
                     /label="Myc-DDK"  
     misc_feature    3827..4412 
                     /label="PolyA signal"  
     misc_feature    3884..3903 
                     /label="Restriction Enzyme"  
     misc_feature    4688..5360 
                     /label="ColE1"  
     misc_feature    5790..6584 
                     /label="Kan/Neo"  
     misc_feature    6619..6921 
                     /label="SV40 ori"  
     misc_feature    7007..7484 
                     /label="f1 ori"  
     misc_feature    1029..1037 
                     /label="Restriction Enzyme"  
     misc_feature    3696..3704 
                     /label="Restriction Enzyme"  
     misc_feature    1029..3696 
                     /label="TPO"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCGG CGCGCCATGA GAGCGCTCGC TGTGCTGTCT GTCACGCTGG TTATGGCCTG 
     1081 CACAGAAGCC TTCTTCCCCT TCATCTCGAG AGGGAAAGAA CTCCTTTGGG GAAAGCCTGA 
     1141 GGAGTCTCGT GTCTCTAGCG TCTTGGAGGA AAGCAAGCGC CTGGTGGACA CCGCCATGTA 
     1201 CGCCACGATG CAGAGAAACC TCAAGAAAAG AGGAATCCTT TCTCCAGCTC AGCTTCTGTC 
     1261 TTTTTCCAAA CTTCCTGAGC CAACAAGCGG AGTGATTGCC CGAGCAGCAG AGATAATGGA 
     1321 AACATCAATA CAAGCGATGA AAAGAAAAGT CAACCTGAAA ACTCAACAAT CACAGCATCC 
     1381 AACGGATGCT TTATCAGAAG ATCTGCTGAG CATCATTGCA AACATGTCTG GATGTCTCCC 
     1441 TTACATGCTG CCCCCAAAAT GCCCAAACAC TTGCCTGGCG AACAAATACA GGCCCATCAC 
     1501 AGGAGCTTGC AACAACAGAG ACCACCCCAG ATGGGGCGCC TCCAACACGG CCCTGGCACG 
     1561 ATGGCTCCCT CCAGTCTATG AGGACGGCTT CAGTCAGCCC CGAGGCTGGA ACCCCGGCTT 
     1621 CTTGTACAAC GGGTTCCCAC TGCCCCCGGT CCGGGAGGTG ACAAGACATG TCATTCAAGT 
     1681 TTCAAATGAG GTTGTCACAG ATGATGACCG CTATTCTGAC CTCCTGATGG CATGGGGACA 
     1741 ATACATCGAC CACGACATCG CGTTCACACC ACAGAGCACC AGCAAAGCTG CCTTCGGGGG 
     1801 AGGGGCTGAC TGCCAGATGA CTTGTGAGAA CCAAAACCCA TGTTTTCCCA TACAACTCCC 
     1861 GGAGGAGGCC CGGCCGGCCG CGGGCACCGC CTGTCTGCCC TTCTACCGCT CTTCGGCCGC 
     1921 CTGCGGCACC GGGGACCAAG GCGCGCTCTT TGGGAACCTG TCCACGGCCA ACCCGCGGCA 
     1981 GCAGATGAAC GGGTTGACCT CGTTCCTGGA CGCGTCCACC GTGTATGGCA GCTCCCCGGC 
     2041 CCTAGAGAGG CAGCTGCGGA ACTGGACCAG TGCCGAAGGG CTGCTCCGCG TCCACGCGCG 
     2101 CCTCCGGGAC TCCGGCCGCG CCTACCTGCC CTTCGTGCCG CCACGCGCGC CTGCGGCCTG 
     2161 TGCGCCCGAG CCCGGCATCC CCGGAGAGAC CCGCGGGCCC TGCTTCCTGG CCGGAGACGG 
     2221 CCGCGCCAGC GAGGTCCCCT CCCTGACGGC ACTGCACACG CTGTGGCTGC GCGAGCACAA 
     2281 CCGCCTGGCC GCGGCGCTCA AGGCCCTCAA TGCGCACTGG AGCGCGGACG CCGTGTACCA 
     2341 GGAGGCGCGC AAGGTCGTGG GCGCTCTGCA CCAGATCATC ACCCTGAGGG ATTACATCCC 
     2401 CAGGATCCTG GGACCCGAGG CCTTCCAGCA GTACGTGGGT CCCTATGAAG GCTATGACTC 
     2461 CACCGCCAAC CCCACTGTGT CCAACGTGTT CTCCACAGCC GCCTTCCGCT TCGGCCATGC 
     2521 CACGATCCAC CCGCTGGTGA GGAGGCTGGA CGCCAGCTTC CAGGAGCACC CCGACCTGCC 
     2581 CGGGCTGTGG CTGCACCAGG CTTTCTTCAG CCCATGGACA TTACTCCGTG GAGGTGGTTT 
     2641 GGACCCACTA ATACGAGGCC TTCTTGCAAG ACCAGCCAAA CTGCAGGTGC AGGATCAGCT 
     2701 GATGAACGAG GAGCTGACGG AAAGGCTCTT TGTGCTGTCC AATTCCAGCA CCTTGGATCT 
     2761 GGCGTCCATC AACCTGCAGA GGGGCCGGGA CCACGGGCTG CCAGGTTACA ATGAGTGGAG 
     2821 GGAGTTCTGC GGCCTGCCTC GCCTGGAGAC CCCCGCTGAC CTGAGCACAG CCATCGCCAG 
     2881 CAGGAGCGTG GCCGACAAGA TCCTGGACTT GTACAAGCAT CCTGACAACA TCGATGTCTG 
     2941 GCTGGGAGGC TTAGCTGAAA ACTTCCTCCC CAGGGCTCGG ACAGGGCCCC TGTTTGCCTG 
     3001 TCTCATTGGG AAGCAGATGA AGGCTCTGCG GGACGGTGAC TGGTTTTGGT GGGAGAACAG 
     3061 CCACGTCTTC ACGGATGCAC AGAGGCGTGA GCTGGAGAAG CACTCCCTGT CTCGGGTCAT 
     3121 CTGTGACAAC ACTGGCCTCA CCAGGGTGCC CATGGATGCC TTCCAAGTCG GCAAATTCCC 
     3181 CGAAGACTTT GAGTCTTGTG ACAGCATCAC TGGCATGAAC CTGGAGGCCT GGAGGGAAAC 
     3241 CTTTCCTCAA GACGACAAGT GTGGCTTCCC AGAGAGCGTG GAGAATGGGG ACTTTGTGCA 
     3301 CTGTGAGGAG TCTGGGAGGC GCGTGCTGGT GTATTCCTGC CGGCACGGGT ATGAGCTCCA 
     3361 AGGCCGGGAG CAGCTCACTT GCACCCAGGA AGGATGGGAT TTCCAGCCTC CCCTCTGCAA 
     3421 AGACTCCGGG AGGCTCCCTC GGGTGACTTG GATCTCCATG TCGCTGGCTG CTCTGCTGAT 
     3481 CGGAGGCTTC GCAGGTCTCA CCTCGACGGT GATTTGCAGG TGGACACGCA CTGGCACTAA 
     3541 ATCCACACTG CCCATCTCGG AGACAGGCGG AGGAACTCCC GAGCTGAGAT GCGGAAAGCA 
     3601 CCAGGCCGTA GGGACCTCAC CGCAGCGGGC CGCAGCTCAG GACTCGGAGC AGGAGAGTGC 
     3661 TGGGATGGAA GGCCGGGATA CTCACAGGCT GCCGAGAGCC CTCGCGGCCG CTCGAGCAGA 
     3721 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     3781 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     3841 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     3901 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     3961 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4021 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4081 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4141 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4201 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4261 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4321 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4381 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4441 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4501 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     4561 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     4621 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     4681 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     4741 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     4801 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     4861 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     4921 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     4981 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5041 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5101 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5161 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5221 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     5281 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     5341 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     5401 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5461 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     5521 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     5581 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     5641 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     5701 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     5761 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     5821 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     5881 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     5941 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6001 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6061 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6121 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6181 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6241 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     6301 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6361 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6421 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6481 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     6541 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     6601 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     6661 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     6721 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     6781 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6841 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     6901 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     6961 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7021 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7081 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7141 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7201 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7261 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     7321 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7381 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7441 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7501 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//