LOCUS       RC222480                9114 bp    DNA     circular  SYN 
DEFINITION  PPIP5K1 (Myc-DDK-tagged)-Human diphosphoinositol pentakisphosphate kinase 1 (PPIP5K1), transcript variant 2
ACCESSION   NM_014659
VERSION     NM_014659.5
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5269..5360 
                     /label="Myc-DDK"  
     misc_feature    5390..5975 
                     /label="PolyA signal"  
     misc_feature    5447..5466 
                     /label="Restriction Enzyme"  
     misc_feature    6251..6923 
                     /label="ColE1"  
     misc_feature    7353..8147 
                     /label="Kan/Neo"  
     misc_feature    8182..8484 
                     /label="SV40 ori"  
     misc_feature    8570..9047 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5244..5251 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5244 
                     /label="PPIP5K1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGGTCATTG ACGGCCAGTG AGGGCGAGAG TACCACGGCC CACTTCTTCC 
     1081 TTGGAGCTGG AGATGAGGGG CTGGGCACCC GTGGAATAGG CATGAGGCCA GAAGAGAGTG 
     1141 ACAGCGAGCT CCTTGAGGAT GAGGAGGATG AAGTGCCTCC TGAACCTCAG ATCATTGTTG 
     1201 GCATCTGTGC CATGACCAAG AAATCCAAGT CCAAGCCAAT GACTCAAATC CTAGAGCGAC 
     1261 TCTGCAGATT TGACTACCTG ACTGTTGTCA TTCTGGGAGA AGATGTAATC CTTAATGAAC 
     1321 CTGTGGAAAA CTGGCCATCC TGCCACTGCC TCATCTCTTT CCACTCCAAA GGCTTTCCTC 
     1381 TGGACAAAGC TGTTGCTTAC TCCAAGCTTC GAAACCCCTT TCTTATCAAT GATCTGGCCA 
     1441 TGCAGTATTA CATCCAAGAT AGGAGGGAGG TGTACCGGAT CCTGCAGGAA GAGGGTATTG 
     1501 ATCTGCCTCG ATATGCTGTG CTCAACCGTG ATCCTGCCCG GCCTGAGGAA TGCAACCTGA 
     1561 TAGAAGGTGA AGACCAAGTA GAGGTCAATG GAGCTGTCTT TCCCAAGCCC TTTGTGGAGA 
     1621 AGCCAGTGAG TGCAGAAGAC CACAATGTTT ACATCTACTA CCCCAGCTCA GCTGGAGGAG 
     1681 GAAGCCAGCG TCTCTTTCGT AAGATTGGCA GCCGAAGCAG TGTTTACTCT CCTGAGAGCA 
     1741 GCGTCCGAAA GACGGGGTCG TACATCTATG AGGAGTTTAT GCCAACAGAT GGCACAGATG 
     1801 TCAAGGTGTA TACAGTGGGG CCAGATTATG CCCATGCTGA AGCTAGAAAA TCTCCAGCTT 
     1861 TGGATGGGAA GGTTGAACGA GACAGTGAGG GGAAAGAGAT TCGATATCCA GTCATGCTGA 
     1921 CTGCCATGGA AAAGCTGGTG GCCAGGAAAG TCTGCGTAGC TTTCAAGCAA ACAGTTTGTG 
     1981 GATTTGACCT TCTTCGTGCC AATGGTCATT CCTTTGTGTG TGATGTCAAT GGCTTTAGTT 
     2041 TTGTCAAGAA CTCGATGAAA TACTACGATG ACTGTGCCAA GATTCTGGGG AACACCATAA 
     2101 TGCGGGAGCT TGCCCCACAG TTCCAGATTC CATGGTCCAT CCCCACGGAG GCTGAGGACA 
     2161 TTCCCATTGT TCCCACCACA TCTGGCACTA TGATGGAACT TCGTTGTGTC ATTGCAATTA 
     2221 TTCGTCATGG GGATCGTACT CCCAAGCAGA AGATGAAGAT GGAAGTGAAA CACCCAAGGT 
     2281 TTTTTGCTCT GTTTGAAAAA CATGGTGGCT ACAAGACAGG GAAATTAAAA CTCAAGCGAC 
     2341 CTGAGCAGCT CCAGGAGGTG CTGGATATCA CAAGGCTGTT GTTGGCTGAA CTGGAGAAAG 
     2401 AACCAGGTGG TGAGATCGAG GAGAAGACTG GAAAACTAGA GCAGCTGAAG TCTGTACTGG 
     2461 AGATGTATGG TCACTTCTCA GGTATAAACC GGAAGGTACA ATTGACTTAC TACCCTCATG 
     2521 GAGTAAAAGC TTCTAATGAG GGGCAAGATC CACAGAGGGA AACTCTGGCC CCATCTCTGT 
     2581 TGCTGGTACT GAAGTGGGGT GGAGAACTGA CTCCTGCTGG CCGTGTTCAG GCTGAGGAGC 
     2641 TGGGGCGAGC TTTTCGCTGC ATGTACCCTG GAGGACAGGG TGACTATGCT GGCTTCCCTG 
     2701 GTTGTGGGCT GCTTCGTCTC CATAGCACTT TCCGCCACGA TCTCAAGATC TATGCCTCTG 
     2761 ATGAGGGTCG TGTTCAGATG ACTGCTGCTG CCTTCGCCAA GGGCCTTCTG GCTCTAGAAG 
     2821 GGGAGCTGAC ACCCATTTTG GTGCAAATGG TGAAGAGTGC CAACATGAAT GGGCTACTGG 
     2881 ACAGCGATGG GGATTCCTTG AGCAGCTGCC AGCACCGGGT GAAGGCTCGG CTGCACCATA 
     2941 TTCTACAGCA GGATGCGCCC TTTGGCCCTG AGGACTACGA TCAGCTGGCT CCCACCAGAA 
     3001 GTACTTCCCT GCTCAACTCC ATGACTATCA TCCAGAATCC TGTGAAGGTC TGTGATCAGG 
     3061 TATTTGCCCT GATCGAAAAC CTCACCCACC AGATCCGGGA ACGAATGCAG GACCCCAGGT 
     3121 CTGTAGACCT GCAGCTCTAC CACAGTGAGA CACTAGAGCT AATGCTACAG CGTTGGAGCA 
     3181 AGCTGGAGCG TGACTTTCGA CAGAAGAGTG GGCGCTATGA TATCAGTAAG ATCCCTGACA 
     3241 TCTATGACTG TGTCAAGTAT GATGTGCAGC ACAATGGGAG TCTGGGACTT CAAGGCACAG 
     3301 CAGAGTTGCT CCGTCTCTCT AAGGCACTGG CTGATGTGGT CATTCCCCAG GAGTACGGGA 
     3361 TCAGTCGGGA GGAGAAACTG GAAATTGCTG TGGGCTTCTG TCTTCCACTG TTGCGGAAGA 
     3421 TACTACTTGA CCTGCAGAGA ACCCACGAGG ATGAGTCTGT CAACAAGCTG CATCCCCTGT 
     3481 ACTCCCGAGG CGTGCTCTCC CCAGGTCGCC ACGTTCGAAC GCGTCTCTAT TTCACCAGTG 
     3541 AGAGCCATGT CCACTCCCTG CTCAGTGTCT TCCGTTATGG AGGACTTCTT GATGAGACCC 
     3601 AGGATGCACA ATGGCAGCGA GCTTTGGATT ATCTTAGTGC CATCTCAGAG CTTAACTACA 
     3661 TGACCCAGAT TGTCATCATG CTTTATGAGG ACAACACACA GGATCCCTTA TCAGAGGAAC 
     3721 GGTTCCATGT GGAGCTACAC TTCAGCCCCG GAGTGAAAGG TGTTGAGGAA GAAGGCAGTG 
     3781 CCCCGGCTGG CTGTGGATTC CGTCCAGCCT CTTCTGAGAA TGAGGAGATG AAAACCAACC 
     3841 AAGGCAGTAT GGAGAACCTG TGTCCAGGAA AGGCATCAGA TGAACCAGAC CGGGCATTGC 
     3901 AGACTTCACC CCAGCCTCCT GAGGGCCCTG GCCTTCCGAG GAGATCACCC CTCATTCGTA 
     3961 ACCGAAAAGC TGGTTCCATG GAGGTACTTT CTGAGACTTC ATCCTCGAGG CCTGGTGGCT 
     4021 ACCGGCTCTT TTCATCTTCA CGGCCACCCA CAGAAATGAA GCAGAGTGGC CTAGGCTCAC 
     4081 AGTGCACAGG GCTGTTCAGC ACCACAGTGC TGGGTGGCTC CTCCAGTGCC CCGAATCTTC 
     4141 AGGACTACGC CCGCAGCCAT GGCAAAAAGC TACCACCTGC CAGTCTGAAG CACCGAGATG 
     4201 GGTTTGAAGG GTGTTCCATG GTGCCTACCA TCTACCCTCT GGAAACACTG CATAATGCCC 
     4261 TTTCCCTACG TCAAGTGAGT GAATTCTTGA GTAGAGTCTG CCAGCGCCAC ACTGATGCCC 
     4321 AGGCACAGGC ATCTGCAGCC CTCTTTGATT CCATGCACAG CAGCCAGGCC TCAGATAACC 
     4381 CATTTTCTCC ACCACGTACT CTTCATTCAC CTCCCCTGCA ACTCCAGCAG CGCTCTGAGA 
     4441 AGCCCCCTTG GTACAGCAGT GGCCCTTCTA GCACTGTGTC CAGTGCTGGT CCTTCTTCCC 
     4501 CTACTACAGT AGATGGTAAC TCCCAATTTG GCTTCAGTGA TCAACCCTCC CTAAATTCAC 
     4561 ACGTGGCTGA AGAACATCAA GGCCTTGGGC TGCTCCAGGA GACCCCTGGG AGTGGAGCAC 
     4621 AAGAGCTCTC CATAGAAGGG GAGCAAGAGC TTTTTGAACC AAATCAGTCC CCACAGGTGC 
     4681 CACCTATGGA AACCAGCCAG CCATACGAGG AGGTCAGCCA GCCATGTCAG GAGGTCCCTG 
     4741 ACATCAGCCA GCCATGCCAG GACATTTCTG AGGCGCTCAG CCAGCCATGT CAGAAGGTCC 
     4801 CTGACATCAG CCAGCAATGC CAGGAGAACC ATGACAATGG TAACCACACA TGCCAGGAGG 
     4861 TCCCTCACAT CAGCCAGCCA TGCCAGAAGT CCAGCCAACT GTGCCAGAAA GTCTCTGAGG 
     4921 AAGTTTGCCA GCTATGTCTG GAGAACTCCG AGGAGGTCAG CCAGCCATGC CAGGGGGTCT 
     4981 CTGTGGAGGT TGGCAAGCTG GTCCATAAGT TCCATGTAGG GGTTGGTAGC TTGGTCCAGG 
     5041 AAACCCTTGT AGAAGTTGGC AGCCCAGCTG AAGAGATCCC TGAGGAGGTC ATCCAGCCAT 
     5101 ACCAGGAGTT CTCTGTGGAG GTTGGCAGGC TGGCCCAGGA GACTTCTGCG ATCAATCTGT 
     5161 TATCTCAGGG CATCCCTGAG ATTGATAAAC CATCCCAAGA GTTCCCTGAG GAGATTGATC 
     5221 TGCAGGCCCA GGAGGTCCCT GAGGAGATAA ATAGCGGACC GACGCGTACG CGGCCGCTCG 
     5281 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     5341 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     5401 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     5461 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5521 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5581 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5641 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5701 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5761 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5821 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5881 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5941 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     6001 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     6061 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     6121 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     6181 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     6241 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     6301 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     6361 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     6421 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6481 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6541 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6601 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6661 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6721 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6781 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6841 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6901 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6961 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     7021 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     7081 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     7141 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     7201 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     7261 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     7321 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     7381 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     7441 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7501 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7561 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7621 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7681 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7741 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7801 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7861 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7921 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7981 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     8041 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     8101 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     8161 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     8221 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     8281 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     8341 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8401 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     8461 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8521 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8581 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8641 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8701 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8761 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8821 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8881 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8941 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     9001 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     9061 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//