LOCUS       RC222444                8154 bp    DNA     circular  SYN 
DEFINITION  ADCY2 (Myc-DDK-tagged)-Human adenylate cyclase 2 (brain) (ADCY2)
ACCESSION   NM_020546
VERSION     NM_020546.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4311..4402 
                     /label="Myc-DDK"  
     misc_feature    4432..5017 
                     /label="PolyA signal"  
     misc_feature    4489..4508 
                     /label="Restriction Enzyme"  
     misc_feature    5293..5965 
                     /label="ColE1"  
     misc_feature    6395..7189 
                     /label="Kan/Neo"  
     misc_feature    7224..7526 
                     /label="SV40 ori"  
     misc_feature    7612..8089 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4293..4299 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4293 
                     /label="ADCY2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGGCAGGAG GCGATGCGGC GCCGCCGCTA CCTGCGGGAC CGCTCCGAGG 
     1081 AGGCGGCGGG CGGCGGAGAC GGGCTGCCGC GGTCCCGGGA CTGGCTCTAC GAGTCCTACT 
     1141 ACTGCATGAG CCAGCAGCAC CCGCTCATCG TCTTCCTGCT GCTCATCGTC ATGGGCTCCT 
     1201 GCCTCGCCCT GCTCGCCGTC TTCTTCGCGC TCGGGCTGGA AGTTGAAGAC CATGTGGCGT 
     1261 TTCTAATAAC AGTTCCAACT GCCCTGGCGA TTTTCTTTGC GATATTTATC CTGGTCTGCA 
     1321 TCGAGTCTGT GTTTAAGAAG CTGCTGCGCC TCTTCTCGTT GGTGATATGG ATATGCCTTG 
     1381 TTGCCATGGG ATACCTGTTC ATGTGTTTTG GAGGCACCGT CTCTCCCTGG GACCAGGTAT 
     1441 CGTTCTTCCT CTTCATCATC TTCGTGTTGT ACACCATGCT GCCCTTCAAC ATGCGAGACG 
     1501 CCATCATTGC CAGCGTCCTC ACCTCCTCCT CCCACACCAT CGTGCTTAGC GTCTGCCTGT 
     1561 CTGCAACACC GGGAGGCAAG GAGCACCTGG TCTGGCAGAT CCTGGCCAAT GTGATCATTT 
     1621 TCATCTGTGG GAACCTGGCG GGAGCCTACC ATAAGCACCT CATGGAACTC GCTCTTCAGC 
     1681 AAACATATCA GGACACCTGT AATTGCATCA AGTCGCGGAT CAAGTTGGAA TTTGAAAAAC 
     1741 GTCAACAGGA GCGGCTTCTG CTCTCCCTGC TGCCGGCCCA CATCGCCATG GAGATGAAAG 
     1801 CGGAGATCAT CCAGAGGCTG CAGGGCCCCA AGGCGGGCCA GATGGAGAAC ACAAATAACT 
     1861 TCCACAACCT GTATGTGAAG CGGCATACAA ACGTGAGCAT CTTATACGCT GACATCGTTG 
     1921 GCTTTACCCG GCTGGCAAGT GACTGCTCCC CGGGAGAACT AGTCCACATG CTGAATGAGC 
     1981 TCTTTGGAAA GTTTGATCAA ATTGCAAAGG AGAATGAATG CATGAGAATT AAAATTTTAG 
     2041 GAGACTGCTA CTACTGTGTA TCTGGACTCC CTATATCTCT CCCTAACCAT GCCAAGAACT 
     2101 GTGTGAAAAT GGGGCTGGAC ATGTGTGAAG CCATAAAGAA AGTGAGGGAT GCTACTGGAG 
     2161 TTGATATCAA CATGCGCGTG GGCGTGCATT CTGGGAATGT CCTGTGTGGC GTGATTGGTC 
     2221 TGCAGAAGTG GCAATATGAT GTGTGGTCAC ATGATGTGAC CTTGGCCAAC CACATGGAAG 
     2281 CTGGAGGGGT CCCTGGACGT GTTCACATTT CTTCTGTCAC CCTGGAGCAC TTGAATGGCG 
     2341 CTTATAAAGT GGAGGAGGGA GATGGTGACA TTAGGGACCC ATATTTAAAA CAGCACCTGG 
     2401 TGAAAACCTA CTTTGTGATC AACCCCAAGG GAGAACGACG GAGCCCCCAG CATCTCTTCA 
     2461 GACCTCGCCA CACCCTTGAT GGAGCCAAAA TGAGGGCCTC GGTCCGCATG ACCCGGTACT 
     2521 TGGAGTCCTG GGGGGCAGCC AAGCCCTTTG CACACCTACA TCACAGGGAC AGCATGACCA 
     2581 CAGAGAACGG CAAGATCAGC ACCACGGATG TACCCATGGG TCAGCATAAT TTTCAAAATC 
     2641 GCACCTTAAG AACCAAGTCA CAAAAGAAGA GATTTGAAGA AGAATTGAAT GAAAGGATGA 
     2701 TTCAAGCAAT TGATGGGATT AATGCACAGA AGCAATGGCT CAAGTCTGAA GACATTCAGA 
     2761 GAATCTCACT GCTTTTCTAT AACAAAGTAC TAGAAAAAGA GTACCGGGCC ACGGCACTGC 
     2821 CAGCGTTCAA GTATTATGTG ACTTGTGCCT GTCTCATATT CTTCTGCATC TTCATTGTGC 
     2881 AGATTCTCGT GCTGCCAAAA ACGTCTGTCC TGGGCATCTC CTTTGGGGCT GCGTTTCTCT 
     2941 TGCTGGCCTT CATCCTCTTC GTCTGCTTTG CTGGACAGCT TCTGCAATGC AGCAAAAAAG 
     3001 CCTCTCCCCT GCTCATGTGG CTTTTGAAGT CCTCGGGCAT CATTGCCAAC CGCCCCTGGC 
     3061 CACGGATCTC TCTCACGATC ATCACCACAG CCATCATATT AATGATGGCC GTGTTCAACA 
     3121 TGTTTTTCCT GAGTGACTCA GAGGAAACAA TCCCTCCAAC TGCCAACACA ACAAACACAA 
     3181 GCTTTTCAGC CTCAAATAAT CAGGTGGCGA TTCTGCGTGC GCAGAATTTA TTTTTCCTCC 
     3241 CGTACTTTAT CTACAGCTGC ATTCTGGGAC TGATATCCTG TTCCGTGTTC CTGCGGGTAA 
     3301 ACTATGAGCT GAAGATGTTG ATCATGATGG TGGCCTTGGT GGGCTACAAC ACCATCCTAC 
     3361 TCCACACCCA CGCCCACGTC CTGGGCGACT ACAGCCAGGT CTTATTTGAG AGACCAGGCA 
     3421 TTTGGAAAGA CCTGAAGACC ATGGGCTCTG TGTCTCTCTC TATATTCTTC ATCACACTGC 
     3481 TTGTTCTGGG TAGACAGAAT GAATATTACT GTAGGTTAGA CTTCTTATGG AAGAACAAAT 
     3541 TCAAAAAAGA GCGGGAGGAG ATAGAGACCA TGGAGAACCT GAACCGCGTG CTGCTGGAGA 
     3601 ACGTGCTTCC CGCGCACGTG GCTGAGCACT TCCTGGCCAG GAGCCTGAAG AATGAGGAGC 
     3661 TATACCACCA GTCCTATGAC TGCGTCTGTG TCATGTTTGC CTCCATTCCG GATTTCAAAG 
     3721 AATTTTATAC AGAATCCGAC GTGAACAAGG AGGGCTTGGA ATGCCTTCGG CTCCTGAACG 
     3781 AGATCATCGC TGACTTTGAT GATCTTCTTT CCAAGCCAAA ATTCAGTGGA GTTGAAAAGA 
     3841 TTAAGACCAT TGGCAGCACA TACATGGCAG CAACAGGTCT GAGCGCTGTG CCCAGCCAGG 
     3901 AGCACTCCCA GGAGCCCGAG CGGCAGTACA TGCACATTGG CACCATGGTG GAGTTTGCTT 
     3961 TTGCCCTGGT AGGGAAGCTG GATGCCATCA ACAAGCACTC CTTCAACGAC TTCAAATTGC 
     4021 GAGTGGGTAT TAACCATGGA CCTGTGATAG CTGGTGTGAT TGGAGCTCAG AAGCCACAAT 
     4081 ATGATATCTG GGGCAACACT GTCAATGTGG CCAGTAGGAT GGACAGCACC GGAGTCCTGG 
     4141 ACAAAATACA GGTTACCGAG GAGACGAGCC TCGTCCTGCA GACCCTCGGA TACACGTGCA 
     4201 CCTGTCGAGG AATAATCAAC GTGAAAGGAA AGGGGGACCT GAAGACGTAC TTTGTAAACA 
     4261 CAGAAATGTC AAGGTCCCTT TCCCAGAGCA ACGTGGCATC CACGCGTACG CGGCCGCTCG 
     4321 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4381 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4441 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4501 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4561 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4621 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4681 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4741 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4801 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4861 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4921 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4981 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5041 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5101 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5161 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5221 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5281 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5341 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5401 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5461 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5521 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5581 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5641 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5701 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5761 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5821 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5881 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5941 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6001 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6061 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6121 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6181 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6241 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6301 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6361 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6421 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6481 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6541 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6601 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6661 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6721 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6781 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6841 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6901 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6961 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7021 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7081 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7141 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7201 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7261 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7321 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7381 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7441 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7501 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7561 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7621 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7681 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7741 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7801 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7861 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7921 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7981 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8041 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8101 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//