LOCUS       RC222431                7923 bp    DNA     circular  SYN 
DEFINITION  NEBL (Myc-DDK-tagged)-Human nebulette (NEBL), transcript variant 1
ACCESSION   NM_006393
VERSION     NM_006393.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4080..4171 
                     /label="Myc-DDK"  
     misc_feature    4201..4786 
                     /label="PolyA signal"  
     misc_feature    4258..4277 
                     /label="Restriction Enzyme"  
     misc_feature    5062..5734 
                     /label="ColE1"  
     misc_feature    6164..6958 
                     /label="Kan/Neo"  
     misc_feature    6993..7295 
                     /label="SV40 ori"  
     misc_feature    7381..7858 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4062..4068 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4062 
                     /label="NEBL"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGGGTCCCT GTATTTGAGG ATATAAAAGA TGAAACTGAA GAAGAAAAGA 
     1081 TAGGGGAAGA AGAAAATGAA GAAGACCAGG TCTTCTATAA GCCTGTTATT GAAGACTTAA 
     1141 GCATGGAATT GGCCAGAAAA TGCACGGAAC TCATTAGCGA TATCCGTTAT AAAGAAGAGT 
     1201 TTAAAAAGTC CAAGGATAAG TGTACATTTG TGACTGACAG TCCTATGCTA AACCATGTAA 
     1261 AAAATATCGG TGCTTTTATT TCTGAGGCAA AATACAAAGG CACCATTAAA GCTGACCTTT 
     1321 CTAATTCTCT TTATAAGCGG ATGCCAGCCA CAATTGACAG TGTTTTTGCA GGAGAAGTTA 
     1381 CACAGCTCCA GAGTGAGGTG GCCTACAAGC AGAAACATGA TGCTGCCAAA GGATTCTCAG 
     1441 ATTATGCCCA CATGAAGGAG CCCCCTGAGG TTAAACATGC CATGGAGGTC AATAAACACC 
     1501 AGAGTAATAT TTCTTATAGG AAAGACGTGC AGGACACCCA CACGTACAGT GCAGAACTTG 
     1561 ACCGACCAGA CATCAAGATG GCAACCCAGA TCTCTAAGAT CATAAGCAAT GCAGAATACA 
     1621 AGAAAGGACA AGGAATAATG AATAAAGAGC CCGCTGTAAT TGGAAGACCA GATTTTGAAC 
     1681 ATGCCGTGGA AGCTTCTAAA CTTTCTAGTC AAATTAAATA CAAAGAAAAA TTCGATAATG 
     1741 AAATGAAGGA TAAGAAACAT CATTACAATC CTCTTGAAAG TGCTTCTTTT AGGCAGAATC 
     1801 AGCTTGCTGC TACACTGGCG AGCAATGTGA AGTACAAGAA AGACATTCAA AATATGCATG 
     1861 ATCCAGTTTC AGATCTCCCA AATTTGTTGT TTTTAGACCA TGTTTTGAAA GCCAGCAAAA 
     1921 TGCTCAGCGG CCGAGAATAT AAAAAGCTCT TTGAGGAAAA CAAAGGAATG TATCATTTTG 
     1981 ATGCAGATGC TGTGGAACAT CTGCACCATA AAGGCAATGC CGTCCTCCAA AGTCAGGTGA 
     2041 AATATAAAGA AGAATATGAG AAAAATAAGG GAAAGCCAAT GCTTGAATTT GTTGAGACAC 
     2101 CATCATATCA AGCTTCAAAG GAGGCTCAAA AGATGCAAAG TGAAAAAGTT TACAAAGAGG 
     2161 ATTTTGAGAA GGAGATTAAA GGAAGGTCAT CACTGGATTT AGACAAGACT CCAGAATTTT 
     2221 TACATGTAAA GTACATCACC AACCTTCTGA GGGAGAAAGA ATATAAAAAA GATTTGGAAA 
     2281 ATGAGATAAA AGGGAAAGGA ATGGAACTTA ATTCAGAAGT TCTTGATATC CAAAGAGCAA 
     2341 AGCGGGCCTC TGAAATGGCA AGTGAGAAAG AATACAAGAA AGACCTGGAG TCAATAATTA 
     2401 AAGGGAAAGG AATGCAAGCT GGCACTGACA CCCTTGAAAT GCAGCATGCC AAGAAGGCTG 
     2461 CAGAGATAGC GAGTGAGAAA GACTATAAAA GAGATCTGGA GACTGAAATT AAAGGGAAAG 
     2521 GGATGCAGGT GAGCACAGAC ACTCTTGATG TCCAGAGAGC TAAGAAAGCA TCCGAGATGG 
     2581 CCAGCCAGAA ACAATACAAG AAGGACTTAG AAAATGAAAT TAAAGGGAAA GGAATGCAAG 
     2641 TGAGCATGGA TATCCCAGAT ATCCTTCGAG CCAAGAGGAC ATCTGAAATC TATAGCCAGA 
     2701 GAAAGTATAA AGATGAAGCA GAGAAGATGC TTTCTAACTA TTCTACCATA GCAGATACTC 
     2761 CTGAAATTCA GAGAATTAAG ACAACTCAAC AAAACATTAG TGCGGTATTT TATAAGAAAG 
     2821 AAGTGGGAGC TGGCACTGCA GTGAAAGATA GCCCAGAGAT CGAACGAGTG AAGAAAAATC 
     2881 AGCAGAATAT TAGTTCAGTG AAATACAAAG AAGAGATTAA ACATGCAACA GCCATTTCTG 
     2941 ATCCTCCAGA ACTAAAGAGA GTTAAAGAAA ACCAGAAGAA CATCAGCAAT CTCCAGTATA 
     3001 AAGAGCAAAA CTACAAGGCC ACTCCGGTAA GCATGACCCC GGAGATAGAG AGAGTGAGGC 
     3061 GAAACCAGGA GCAGCTGAGT GCGGTAAAAT ATAAGGGAGA ACTTCAACGG GGAACTGCAA 
     3121 TTTCTGATCC ACCAGAGCTG AAGAGGGCAA AAGAAAACCA GAAAAACATC AGCAATGTTT 
     3181 ATTACAGAGG TCAGCTGGGA AGAGCTACCA CTTTAAGTGT AACTCCTGAA ATGGAAAGAG 
     3241 TGAAGAAGAA TCAAGAAAAT ATTAGCTCGG TAAAATATAC CCAGGACCAT AAACAGATGA 
     3301 AAGGTAGACC AAGTCTGATT TTAGATACAC CTGCTATGAG ACATGTTAAA GAAGCACAAA 
     3361 ATCATATTTC AATGGTAAAA TACCATGAAG ATTTTGAAAA AACAAAGGGG AGAGGCTTTA 
     3421 CTCCCGTCGT GGACGATCCT GTGACAGAGA GAGTGAGGAA GAACACCCAG GTGGTCAGCG 
     3481 ATGCTGCCTA TAAAGGGGTC CACCCTCACA TCGTGGAGAT GGACAGGAGA CCTGGAATCA 
     3541 TTGTTGACCT CAAAGTTTGG CGCACAGATC CTGGCTCCAT CTTCGACCTT GATCCCCTGG 
     3601 AAGACAATAT TCAGTCTAGA AGTCTCCATA TGCTCTCTGA AAAGGCGAGT CACTATAGGC 
     3661 GACACTGGTC TCGATCCCAT TCCAGCAGTA CTTTCGGTAC AGGTCTCGGA GACGACAGGT 
     3721 CAGAAATCTC CGAGATTTAC CCTAGCTTTT CATGCTGCAG TGAGGTAACA AGACCGTCTG 
     3781 ATGAAGGAGC ACCTGTTCTT CCCGGAGCCT ATCAGCAAAG CCATTCCCAA GGCTATGGCT 
     3841 ACATGCACCA GACCAGTGTG TCATCCATGA GATCAATGCA GCATTCACCA AATCTAAGGA 
     3901 CCTACCGAGC CATGTACGAT TACAGTGCCC AGGATGAAGA CGAGGTCTCC TTTAGAGACG 
     3961 GCGACTACAT CGTCAACGTG CAGCCTATTG ACGATGGCTG GATGTACGGC ACAGTGCAGA 
     4021 GAACAGGGAG AACAGGAATG CTCCCAGCGA ATTACATTGA GTTTGTTAAT ACGCGTACGC 
     4081 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4141 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4201 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4261 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4321 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4381 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4441 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4501 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4561 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4621 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4681 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4741 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4801 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     4861 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     4921 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     4981 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5041 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5101 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5161 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5221 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5281 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5341 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5401 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5461 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5521 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5581 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     5641 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     5701 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     5761 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     5821 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     5881 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     5941 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6001 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6061 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6121 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6181 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6241 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6301 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6361 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6421 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6481 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6541 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6601 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     6661 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     6721 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     6781 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     6841 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     6901 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     6961 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7021 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7081 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7141 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7201 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7261 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7321 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7381 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7441 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7501 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7561 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7621 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     7681 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     7741 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     7801 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     7861 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     7921 TTT
//