LOCUS       RC222391                8985 bp    DNA     circular  SYN 
DEFINITION  MED23 (Myc-DDK-tagged)-Human mediator complex subunit 23 (MED23), transcript variant 1
ACCESSION   NM_004830
VERSION     NM_004830.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5142..5233 
                     /label="Myc-DDK"  
     misc_feature    5263..5848 
                     /label="PolyA signal"  
     misc_feature    5320..5339 
                     /label="Restriction Enzyme"  
     misc_feature    6124..6796 
                     /label="ColE1"  
     misc_feature    7226..8020 
                     /label="Kan/Neo"  
     misc_feature    8055..8357 
                     /label="SV40 ori"  
     misc_feature    8443..8920 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5124..5130 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5124 
                     /label="MED23"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGACGCAA CTGCAGAGCA TTTTCGAAGA GGTGGTGAAA ACGGAAGTTA 
     1081 TAGAAGAGGC TTTTCCTGGC ATGTTTATGG ATACTCCTGA AGATGAGAAA ACAAAACTAA 
     1141 TTAGCTGTTT GGGGGCCTTC AGACAGTTTT GGGGTGGTCT TTCTCAGGAG TCTCATGAAC 
     1201 AGTGTATCCA GTGGATTGTT AAGTTTATTC ATGGTCAGCA TAGTCCTAAA AGAATTTCTT 
     1261 TTCTTTATGA CTGCTTAGCA ATGGCAGTTG AGACTGGTCT CCTTCCACCC AGGCTGGTTT 
     1321 GTGAATCCCT GATAAACTCT GACACTCTTG AGTGGGAAAG AACACAGCTT TGGGCCTTAA 
     1381 CATTTAAACT GGTTCGGAAA ATAATTGGGG GAGTGGATTA CAAGGGTGTT CGAGATCTCT 
     1441 TAAAAGTGAT TTTGGAGAAG ATTTTGACAA TTCCTAATAC AGTGAGCTCT GCTGTTGTAC 
     1501 AGCAGCTTCT GGCAGCAAGA GAGGTTATAG CATATATCTT GGAAAGAAAT GCCTGCTTAT 
     1561 TACCAGCCTA TTTTGCAGTC ACTGAGATCA GGAAACTGTA TCCTGAAGGC AAACTTCCAC 
     1621 ACTGGTTACT TGGAAACCTA GTATCAGACT TTGTGGATAC CTTCAGGCCC ACAGCAAGGA 
     1681 TAAACTCCAT TTGTGGTCGC TGTAGTCTTC TGCCAGTTGT AAATAATTCG GGTGCCATTT 
     1741 GTAATTCATG GAAACTGGAT CCTGCTACTC TTCGTTTTCC TTTGAAAGGC CTTTTGCCAT 
     1801 ATGATAAGGA TCTGTTTGAA CCACAGACTG CTTTGTTGAG ATATGTATTG GAGCAGCCTT 
     1861 ATTCCAGGGA TATGGTCTGC AATATGCTAG GTTTAAATAA GCAGCACAAG CAGCGCTGCC 
     1921 CTGTGCTGGA GGACCAGTTG GTGGATCTGG TTGTTTATGC CATGGAGCGA TCTGAGACCG 
     1981 AGGAGAAGTT TGACGATGGG GGAACAAGCC AACTCCTGTG GCAGCATCTC TCAAGTCAGC 
     2041 TCATTTTCTT TGTGCTTTTC CAGTTTGCAA GTTTTCCACA TATGGTGCTT TCTCTTCATC 
     2101 AGAAGTTAGC AGGGCGAGGA CTGATTAAAG GCAGAGATCA TCTTATGTGG GTTCTCCTGC 
     2161 AATTCATTTC TGGAAGTATT CAGAAAAATG CACTAGCTGA TTTTCTCCCT GTGATGAAGC 
     2221 TCTTCGACTT GCTATACCCA GAAAAAGAAT ATATCCCAGT TCCTGATATT AACAAACCCC 
     2281 AGTCAACCCA TGCCTTTGCA ATGACCTGTA TTTGGATTCA TCTCAATAGA AAAGCTCAAA 
     2341 ATGACAACTC CAAGCTACAG ATTCCAATAC CTCATTCCCT AAGACTTCAC CATGAGTTCC 
     2401 TGCAGCAGAG TCTAAGAAAT AAAAGTTTAC AGATGAATGA CTATAAGATT GCTCTATTGT 
     2461 GTAATGCATA CTCTACAAAT TCAGAATGTT TTACATTACC CATGGGAGCT CTGGTAGAAA 
     2521 CTATTTATGG AAATGGAATT ATGAGGATAC CTCTCCCTGG AACAAACTGT ATGGCTTCAG 
     2581 GATCTATTAC CCCCTTACCT ATGAACCTCC TGGATTCACT GACAGTTCAT GCCAAAATGA 
     2641 GCCTTATTCA CAGCATTGCA ACCAGGGTGA TAAAACTTGC TCATGCAAAG TCCAGTGTGG 
     2701 CCTTGGCTCC AGCCCTAGTG GAAACTTACA GTCGTTTATT GGTCTATATG GAAATAGAGT 
     2761 CTTTGGGCAT CAAAGGATTT ATCAGTCAGC TTTTGCCAAC TGTTTTCAAA TCACATGCAT 
     2821 GGGGGATCTT ACACACACTC CTTGAGATGT TTAGCTACCG GATGCATCAT ATTCAGCCTC 
     2881 ATTACAGAGT TCAGCTCCTG AGTCATCTTC ATACTTTGGC TGCAGTTGCA CAAACAAACC 
     2941 AGAACCAGCT CCATCTTTGT GTCGAGAGCA CTGCTCTCAG GCTTATAACA GCATTAGGTA 
     3001 GCTCAGAGGT ACAACCGCAG TTTACACGCT TCCTTAGTGA TCCCAAAACA GTGCTCTCAG 
     3061 CAGAATCTGA AGAACTGAAC CGAGCCTTGA TATTGACCTT GGCTAGAGCA ACTCATGTAA 
     3121 CAGATTTTTT TACAGGCTCT GATTCAATTC AGGGAACTTG GTGTAAAGAC ATACTTCAGA 
     3181 CCATCATGAG TTTCACTCCT CATAATTGGG CTTCACACAC CCTGAGCTGT TTTCCAGGCC 
     3241 CACTACAGGC ATTCTTCAAA CAAAATAATG TGCCTCAGGA AAGCCGTTTT AATCTGAAAA 
     3301 AAAATGTGGA GGAGGAGTAT AGGAAGTGGA AGTCAATGAG CAACGAAAAC GACATTATTA 
     3361 CCCACTTCTC TATGCAGGGC TCCCCTCCTC TCTTTCTTTG TCTTCTCTGG AAAATGCTCT 
     3421 TGGAAACAGA TCATATTAAT CAGATTGGCT ATAGAGTATT AGAGAGAATT GGAGCCAGGG 
     3481 CCTTGGTAGC CCATGTGAGG ACATTTGCAG ATTTCCTGGT ATATGAGTTT TCTACATCAG 
     3541 CAGGGGGTCA GCAACTCAAT AAATGCATTG AAATTCTTAA TGACATGGTA TGGAAGTATA 
     3601 ACATTGTTAC ACTGGACAGA TTAATTCTCT GCCTGGCCAT GCGTAGTCAC GAAGGAAATG 
     3661 AAGCCCAGGT TTGTTATTTC ATAATTCAGT TGCTGTTACT CAAACCAAAC GATTTTAGAA 
     3721 ATCGAGTAAG TGACTTTGTG AAGGAAAATT CCCCAGAGCA CTGGTTACAG AATGACTGGC 
     3781 ACACCAAGCA CATGAATTAT CACAAGAAAT ATCCAGAGAA GTTGTATTTT GAGGGCCTCG 
     3841 CGGAACAGGT GGATCCTCCT GTACAGATCC AGTCTCCCTA TCTGCCCATC TATTTTGGGA 
     3901 ATGTGTGTCT TCGATTCCTT CCAGTATTTG ATATAGTAAT CCACAGATTT TTAGAGTTGC 
     3961 TTCCGGTATC CAAATCACTG GAGACTCTAC TGGATCATCT AGGAGGCTTA TATAAATTTC 
     4021 ATGATCGTCC AGTGACTTAT CTGTATAACA CTCTGCACTA TTATGAAATG CACCTGAGAG 
     4081 ACCGCGCATT TCTCAAACGA AAACTCGTCC ATGCGATCAT TGGCTCTCTG AAGGATAATC 
     4141 GACCGCAGGG CTGGTGTCTA AGTGACACTT ACCTGAAATG CGCTATGAAT GCACGAGAGG 
     4201 AAAATCCTTG GGTTCCAGAT GACACCTACT ATTGCAGATT GATTGGCAGA CTAGTCGATA 
     4261 CGATGGCTGG CAAATCTCCT GGTCCCTTTC CAAACTGTGA CTGGAGATTC AATGAGTTTC 
     4321 CCAACCCAGC TGCCCATGCT CTCCATGTTA CTTGTGTGGA GCTCATGGCC TTGGCAGTTT 
     4381 CAGGCAAAGA AGTTGGGAAT GCCCTTCTAA ATGTTGTCCT AAAAAGTCAG CCTTTAGTGC 
     4441 CAAGAGAGAA CATTACAGCA TGGATGAATG CAATTGGTTT GATCATCACT GCCCTACCAG 
     4501 AGCCATATTG GATTGTTCTT CATGATCGAA TTGTGAGTGT CATCAGCAGC CCCAGCTTGA 
     4561 CGTCTGAAAC AGAGTGGGTT GGCTATCCAT TCCGCCTCTT TGATTTCACT GCCTGTCATC 
     4621 AGTCCTACTC TGAGATGAGT TGTAGCTATA CGTTAGCTCT TGCACATGCT GTGTGGCACC 
     4681 ATTCTAGCAT CGGACAACTT TCTCTCATTC CAAAGTTTCT TACTGAAGTA CTTCTTCCTA 
     4741 TAGTGAAGAC CGAATTCCAG TTGCTTTATG TATACCATCT TGTTGGACCA TTTTTACAAA 
     4801 GATTTCAGCA AGAGAGAACT CGTTGTATGA TAGAGATTGG TGTGGCGTTT TATGACATGC 
     4861 TGCTGAATGT TGACCAGTGT AGCACCCATT TAAATTACAT GGATCCCATC TGTGACTTCC 
     4921 TCTATCACAT GAAGTATATG TTTACTGGTG ACAGCGTGAA AGAGCAAGTA GAGAAGATTA 
     4981 TCTGTAACTT AAAACCAGCT TTAAAACTTC GTCTTCGATT CATCACACAC ATTAGCAAGA 
     5041 TGGAGCCAGC TGCAGTGCCT CCACAAGCCA TGAACAGTGG GTCTCCAGCA CCTCAGTCTA 
     5101 ATCAGGTGCC CGTGTCTTTA CCAGTAACTC AGACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     5161 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     5221 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     5281 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     5341 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5401 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5461 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5521 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5581 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5641 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5701 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5761 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5821 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5881 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5941 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     6001 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     6061 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     6121 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     6181 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     6241 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     6301 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6361 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6421 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6481 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6541 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6601 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6661 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6721 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6781 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6841 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6901 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6961 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     7021 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     7081 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     7141 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     7201 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     7261 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     7321 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7381 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7441 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7501 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7561 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7621 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7681 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7741 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7801 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7861 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7921 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7981 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     8041 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     8101 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     8161 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     8221 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8281 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8341 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8401 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8461 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8521 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8581 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8641 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8701 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8761 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8821 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8881 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8941 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//