LOCUS       RC222346                7908 bp    DNA     circular  SYN 
DEFINITION  HLTF (Myc-DDK-tagged)-Human helicase-like transcription factor (HLTF), transcript variant 2
ACCESSION   NM_139048
VERSION     NM_139048.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4065..4156 
                     /label="Myc-DDK"  
     misc_feature    4186..4771 
                     /label="PolyA signal"  
     misc_feature    4243..4262 
                     /label="Restriction Enzyme"  
     misc_feature    5047..5719 
                     /label="ColE1"  
     misc_feature    6149..6943 
                     /label="Kan/Neo"  
     misc_feature    6978..7280 
                     /label="SV40 ori"  
     misc_feature    7366..7843 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4047..4053 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4047 
                     /label="HLTF"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCTGGATG TTCAAGAGGG ATCCAGTTTG GAAGTACTTG CAGACTGTCC 
     1081 AGTATGGAGT TCATGGAAAT TTTCCACGCC TCTCATATCC AACTTTCTTT CCACGTTTTG 
     1141 AATTCCAAGA TGTTATCCCT CCAGATGACT TTCTAACTAG TGATGAAGAA GTAGATTCCG 
     1201 TTTTATTTGG AAGTTTGAGA GGTCATGTGG TTGGACTACG CTATTACACG GGAGTAGTTA 
     1261 ATAATAATGA AATGGTTGCA TTACAACGAG ATCCTAATAA CCCTTATGAT AAGAATGCAA 
     1321 TTAAAGTAAA CAATGTGAAT GGAAATCAAG TTGGCCATTT AAAGAAAGAG CTTGCAGGTG 
     1381 CTTTGGCCTA TATCATGGAC AACAAATTGG CACAAATTGA AGGGGTAGTT CCTTTTGGTG 
     1441 CAAACAATGC TTTTACCATG CCTCTGCATA TGACTTTTTG GGGAAAAGAA GAAAATAGAA 
     1501 AAGCGGTTTC AGATCAGTTG AAGAAACATG GATTTAAATT GGGTCCTGCA CCAAAAACTT 
     1561 TAGGATTCAA TTTGGAAAGT GGTTGGGGCT CTGGAAGAGC TGGACCAAGC TATAGTATGC 
     1621 CAGTGCATGC TGCAGTACAG ATGACAACTG AACAGCTTAA AACAGAATTT GACAAATTGT 
     1681 TTGAAGATTT AAAAGAAGAT GATAAAACCC ATGAAATGGA ACCAGCTGAG GCTATTGAAA 
     1741 CACCACTGCT TCCACATCAA AAACAAGCTC TAGCTTGGAT GGTGTCACGG GAAAATAGCA 
     1801 AAGAACTTCC ACCATTCTGG GAACAGCGAA ATGACTTATA CTATAACACA ATAACAAATT 
     1861 TTTCTGAGAA GGACCGACCA GAAAATGTCC ATGGAGGAAT TTTAGCTGAT GATATGGGTT 
     1921 TGGGTAAAAC TCTTACGGCC ATTGCAGTAA TCCTTACCAA CTTCCATGAT GGCAGACCTC 
     1981 TTCCTATTGA AAGAGTTAAA AAGAATCTAC TGAAGAAGGA ATATAATGTT AACGATGACT 
     2041 CTATGAAACT TGGAGGAAAC AATACCAGTG AAAAGGCAGA TGGACTAAGC AAAGACGCAT 
     2101 CTAGATGTAG TGAACAACCC AGTATTTCAG ATATCAAGGA GAAGAGTAAG TTTCGCATGT 
     2161 CAGAATTGTC TAGCTCCCGC CCCAAAAGAA GAAAAACTGC TGTCCAGTAC ATAGAAAGCA 
     2221 GTGATTCAGA GGAAATTGAA ACAAGTGAAT TGCCGCAGAA AATGAAAGGC AAACTGAAAA 
     2281 ATGTACAGTC TGAAACTAAA GGCAGGGCGA AAGCAGGATC TTCTAAGGTT ATAGAAGATG 
     2341 TGGCATTTGC ATGTGCATTA ACTTCATCTG TTCCTACAAC AAAAAAGAAA ATGTTGAAAA 
     2401 AGGGAGCTTG TGCAGTGGAG GGGTCAAAGA AAACTGATGT TGAGGAGAGA CCAAGAACAA 
     2461 CACTGATCAT CTGTCCGCTT TCTGTGTTAA GCAACTGGAT TGACCAGTTT GGACAACATA 
     2521 TAAAATCAGA TGTACACTTG AATTTTTATG TTTATTATGG TCCTGATCGT ATTAGAGAAC 
     2581 CGGCCTTACT TTCAAAACAG GATATTGTTT TGACTACGTA TAATATTTTA ACTCATGACT 
     2641 ATGGAACTAA AGGAGATAGT CCATTACATA GCATAAGGTG GCTAAGAGTG ATCCTGGATG 
     2701 AAGGACATGC CATACGAAAT CCAAATGCTC AGCAGACAAA AGCTGTACTT GACTTAGAAT 
     2761 CAGAAAGAAG ATGGGTTTTG ACAGGTACTC CAATCCAGAA TTCTTTAAAG GACTTGTGGT 
     2821 CTCTTCTTTC CTTTTTAAAA CTTAAACCAT TTATTGATAG AGAATGGTGG CATAGAACAA 
     2881 TACAGCGTCC TGTCACAATG GGAGATGAAG GAGGACTTAG GCGTTTACAG TCCCTAATTA 
     2941 AAAATATTAC ACTTAGAAGA ACAAAGACAA GCAAAATTAA AGGAAAACCT GTTTTGGAGT 
     3001 TACCAGAACG TAAAGTATTT ATTCAGCACA TTACACTTTC AGATGAAGAG AGAAAGATTT 
     3061 ATCAGTCTGT GAAAAATGAA GGCAGAGCCA CTATTGGAAG GTATTTTAAT GAAGGGACTG 
     3121 TCCTGGCACA TTATGCAGAT GTCCTGGGTC TTTTGCTTAG ACTGCGGCAA ATTTGTTGCC 
     3181 ATACTTACCT TCTTACAAAT GCAGTGTCTT CCAATGGCCC CTCAGGAAAT GATACACCTG 
     3241 AAGAACTGAG AAAGAAGTTA ATAAGGAAGA TGAAGTTAAT TCTGAGCTCA GGTTCAGATG 
     3301 AGGAATGTGC AATTTGCCTG GATTCTTTAA CAGTTCCTGT GATAACACAT TGTGCACATG 
     3361 TATTTTGTAA ACCCTGTATT TGCCAAGTCA TTCAGAATGA GCAGCCACAT GCTAAATGCC 
     3421 CTTTATGCAG AAATGATATA CATGAAGATA ATTTATTAGA ATGTCCTCCA GAAGAATTAG 
     3481 CACGTGACAG TGAGAAAAAG TCTGATATGG AATGGACATC CAGTTCAAAG ATTAATGCGC 
     3541 TAATGCACGC ATTGACTGAC TTAAGAAAGA AGAATCCCAA CATAAAAAGT TTGGTTGTTT 
     3601 CTCAGTTTAC AACATTCCTG TCTTTAATAG AAATACCACT TAAAGCCTCT GGATTTGTGT 
     3661 TTACTCGTTT GGATGGTTCC ATGGCCCAAA AGAAAAGAGT TGAATCAATT CAGTGTTTTC 
     3721 AAAACACTGA AGCAGGATCT CCAACTATAA TGCTTCTGTC CTTAAAAGCA GGTGGAGTTG 
     3781 GTTTGAATCT GTCTGCAGCT TCTCGAGTGT TTTTAATGGA TCCAGCCTGG AATCCTGCTG 
     3841 CTGAAGATCA GTGCTTTGAC AGATGCCATA GACTTGGTCA GAAGCAAGAA GTTATCATCA 
     3901 CAAAATTCAT TGTAAAGGAC TCTGTTGAAG AAAATATGCT GAAAATACAA AACAAAAAGA 
     3961 GAGAACTTGC AGCAGGAGCC TTTGGAACTA AAAAACCAAA TGCTGACGAA ATGAAACAAG 
     4021 CCAAAATTAA TGAAATCAGA ACATTAATTG ACTTAACGCG TACGCGGCCG CTCGAGCAGA 
     4081 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4141 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4201 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4261 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4321 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4381 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4441 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4501 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4561 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4621 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4681 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4741 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4801 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4861 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     4921 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     4981 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5041 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5101 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5161 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5221 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5281 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5341 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5401 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5461 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5521 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5581 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     5641 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     5701 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     5761 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5821 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     5881 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     5941 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6001 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6061 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6121 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6181 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6241 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6301 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6361 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6421 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6481 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6541 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6601 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     6661 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6721 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6781 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6841 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     6901 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     6961 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7021 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7081 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7141 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7201 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7261 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7321 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7381 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7441 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7501 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7561 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7621 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     7681 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7741 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7801 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7861 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//