LOCUS       RC222345                7683 bp    DNA     circular  SYN 
DEFINITION  PDE11A (Myc-DDK-tagged)-Human phosphodiesterase 11A (PDE11A), transcript variant 1
ACCESSION   NM_001077196
VERSION     NM_001077196.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3840..3931 
                     /label="Myc-DDK"  
     misc_feature    3961..4546 
                     /label="PolyA signal"  
     misc_feature    4018..4037 
                     /label="Restriction Enzyme"  
     misc_feature    4822..5494 
                     /label="ColE1"  
     misc_feature    5924..6718 
                     /label="Kan/Neo"  
     misc_feature    6753..7055 
                     /label="SV40 ori"  
     misc_feature    7141..7618 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3822..3828 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3822 
                     /label="PDE11A"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAGCCTCC CGCCTGGACT TTGGGGAGGT GGAAACTTTC CTGGACAGGC 
     1081 ACCCAGAGTT GTTTGAAGAT TACTTGATGC GGAAGGGGAA GCAGGAGATG GTTGAAAAGT 
     1141 GGCTGCAGAG GCACAGTCAG GGTCAGGGGG CTTTAGGTCC AAGGCCCTCT TTGGCTGGTA 
     1201 CCAGCAGCTT GGCTCACAGC ACCTGCAGAG GTGGCAGCAG CGTTGGTGGT GGCACTGGAC 
     1261 CAAATGGCTC TGCCCACAGC CAGCCCCTTC CCGGTGGCGG GGACTGTGGT GGGGTTCCCT 
     1321 TGAGTCCCAG CTGGGCCGGT GGCAGCAGGG GCGATGGGAA CCTGCAGCGG AGAGCTTCTC 
     1381 AGAAAGAGCT AAGGAAGAGT TTTGCCCGCT CCAAGGCCAT CCACGTGAAC AGGACCTACG 
     1441 ATGAACAGGT GACCTCCCGG GCTCAGGAAC CCCTGAGTAG TGTACGACGG AGGGCACTTC 
     1501 TCCGGAAGGC AAGCTCCCTG CCCCCCACCA CAGCCCATAT TCTCAGTGCG CTGCTGGAAT 
     1561 CGAGAGTGAA TCTGCCTCAG TATCCCCCTA CAGCCATCGA CTACAAGTGC CATCTGAAAA 
     1621 AGCATAATGA GCGTCAGTTC TTTCTGGAAT TGGTCAAAGA TATCTCCAAT GACCTTGACC 
     1681 TCACCAGCCT GAGCTACAAG ATTCTCATCT TTGTCTGCCT TATGGTGGAT GCTGACCGCT 
     1741 GCTCTCTTTT CCTGGTGGAA GGGGCAGCTG CTGGCAAGAA GACCTTGGTC TCCAAATTCT 
     1801 TTGATGTGCA TGCAGGAACC CCTCTGCTGC CTTGCAGCAG CACAGAGAAC TCAAATGAGG 
     1861 TGCAGGTCCC CTGGGGCAAA GGTATCATTG GCTATGTCGG GGAGCATGGA GAAACGGTCA 
     1921 ACATTCCTGA TGCCTACCAG GATCGACGAT TCAATGATGA AATCGACAAG CTAACTGGAT 
     1981 ACAAGACAAA ATCATTATTG TGCATGCCTA TCCGAAGCAG TGATGGTGAG ATTATTGGTG 
     2041 TGGCCCAAGC GATAAATAAG ATTCCTGAAG GAGCTCCATT TACTGAAGAT GATGAAAAAG 
     2101 TTATGCAGAT GTATCTTCCA TTTTGTGGAA TCGCCATATC TAACGCTCAG CTCTTTGCTG 
     2161 CCTCAAGGAA AGAATATGAA AGAAGCAGAG CTTTGCTAGA GGTGGTTAAT GACCTCTTTG 
     2221 AAGAACAGAC TGACCTGGAG AAAATTGTCA AGAAAATAAT GCATCGGGCC CAAACTCTGC 
     2281 TGAAATGTGA ACGCTGTTCT GTTTTACTCC TAGAGGACAT CGAATCACCA GTGGTGAAAT 
     2341 TTACCAAATC CTTTGAATTG ATGTCCCCAA AGTGCAGTGC TGATGCTGAG AACAGTTTCA 
     2401 AAGAAAGCAT GGAGAAATCA TCATACTCCG ACTGGCTAAT AAATAACAGC ATTGCTGAGC 
     2461 TGGTTGCTTC AACAGGCCTT CCAGTGAACA TCAGTGATGC CTACCAGGAT CCGCGCTTTG 
     2521 ATGCAGAGGC AGACCAGATA TCTGGTTTTC ACATAAGATC TGTTCTTTGT GTCCCTATTT 
     2581 GGAATAGCAA CCACCAAATA ATTGGAGTGG CTCAAGTGTT AAACAGACTT GATGGGAAAC 
     2641 CTTTTGATGA TGCAGATCAA CGACTTTTTG AGGCTTTTGT CATCTTTTGT GGACTTGGCA 
     2701 TCAACAACAC AATTATGTAT GATCAAGTGA AGAAGTCCTG GGCCAAGCAG TCTGTGGCTC 
     2761 TTGATGTGCT ATCATACCAT GCAACATGTT CAAAAGCTGA AGTTGACAAG TTTAAGGCAG 
     2821 CCAACATCCC TCTGGTGTCA GAACTTGCCA TCGATGACAT TCATTTTGAT GACTTTTCTC 
     2881 TCGACGTTGA TGCCATGATC ACAGCTGCTC TCCGGATGTT CATGGAGCTG GGGATGGTAC 
     2941 AGAAATTTAA AATTGACTAT GAGACACTGT GTAGGTGGCT TTTGACAGTG AGGAAAAACT 
     3001 ATCGGATGGT TCTATACCAC AACTGGAGAC ATGCCTTCAA CGTGTGTCAG CTGATGTTCG 
     3061 CGATGTTAAC CACTGCTGGG TTTCAAGACA TTCTGACCGA GGTGGAAATT TTAGCGGTGA 
     3121 TTGTGGGATG CCTGTGTCAT GACCTCGACC ACAGGGGAAC CAACAATGCC TTCCAAGCTA 
     3181 AGAGTGGCTC TGCCCTGGCC CAACTCTATG GAACCTCTGC TACCTTGGAG CATCACCATT 
     3241 TCAACCACGC CGTGATGATC CTTCAAAGTG AGGGTCACAA TATCTTTGCT AACCTGTCCT 
     3301 CCAAGGAATA TAGTGACCTT ATGCAGCTTT TGAAGCAGTC AATATTGGCA ACAGACCTCA 
     3361 CGCTGTACTT TGAGAGGAGA ACTGAATTCT TTGAACTTGT CAGTAAAGGA GAATACGATT 
     3421 GGAACATCAA AAACCATCGT GATATATTTC GATCAATGTT AATGACAGCC TGTGACCTTG 
     3481 GAGCCGTGAC CAAACCGTGG GAGATCTCCA GACAGGTGGC AGAACTTGTA ACCAGTGAGT 
     3541 TCTTCGAACA AGGAGATCGG GAGAGATTAG AGCTCAAACT CACTCCTTCA GCAATTTTTG 
     3601 ATCGGAACCG GAAGGATGAA CTGCCTCGGT TGCAACTGGA GTGGATTGAT AGCATCTGCA 
     3661 TGCCTTTGTA TCAGGCACTG GTGAAGGTCA ACGTGAAACT GAAGCCGATG CTAGATTCAG 
     3721 TAGCTACAAA CAGAAGTAAG TGGGAAGAGC TACACCAAAA ACGACTGCTG GCCTCAACTG 
     3781 CCTCATCCTC CTCCCCTGCC AGTGTTATGG TAGCCAAGGA AGACAGGAAC ACGCGTACGC 
     3841 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     3901 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     3961 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4021 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4081 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4141 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4201 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4261 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4321 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4381 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4441 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4501 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4561 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     4621 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     4681 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     4741 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     4801 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     4861 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     4921 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     4981 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5041 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5101 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5161 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5221 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5281 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5341 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     5401 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     5461 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     5521 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     5581 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     5641 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     5701 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     5761 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     5821 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     5881 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     5941 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6001 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6061 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6121 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6181 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6241 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6301 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6361 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     6421 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     6481 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     6541 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     6601 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     6661 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     6721 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     6781 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     6841 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     6901 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     6961 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7021 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7081 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7141 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7201 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7261 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7321 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7381 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     7441 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     7501 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     7561 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     7621 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     7681 TTT
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