LOCUS       RC222281                8886 bp    DNA     circular  SYN 
DEFINITION  RPTOR (Myc-DDK-tagged)-Human regulatory associated protein of MTOR, complex 1 (RPTOR), transcript variant 1
ACCESSION   NM_020761
VERSION     NM_020761.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5043..5134 
                     /label="Myc-DDK"  
     misc_feature    5164..5749 
                     /label="PolyA signal"  
     misc_feature    5221..5240 
                     /label="Restriction Enzyme"  
     misc_feature    6025..6697 
                     /label="ColE1"  
     misc_feature    7127..7921 
                     /label="Kan/Neo"  
     misc_feature    7956..8258 
                     /label="SV40 ori"  
     misc_feature    8344..8821 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5025..5031 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5025 
                     /label="RPTOR"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGTCCGAA ATGCTGCAAT CGCCTCTTCT GGGCCTGGGG GAGGAAGATG 
     1081 AGGCTGATCT TACAGACTGG AACCTACCTT TGGCTTTTAT GAAAAAGAGG CACTGTGAGA 
     1141 AAATTGAAGG CTCCAAATCC TTAGCTCAGA GCTGGAGGAT GAAGGATCGG ATGAAGACAG 
     1201 TCAGTGTTGC CTTAGTTTTG TGCCTGAATG TTGGTGTGGA CCCTCCCGAT GTGGTGAAGA 
     1261 CCACGCCCTG TGCACGCTTG GAATGCTGGA TCGATCCTCT GTCGATGGGT CCTCAGAAAG 
     1321 CTCTGGAAAC CATCGGTGCA AATTTACAGA AGCAGTACGA GAACTGGCAG CCAAGGGCCC 
     1381 GGTACAAGCA GAGCCTTGAC CCAACTGTGG ATGAAGTCAA GAAGCTCTGC ACGTCCTTAC 
     1441 GTCGCAACGC CAAGGAGGAG CGAGTCCTCT TTCACTACAA TGGCCACGGG GTGCCCCGGC 
     1501 CCACAGTCAA CGGGGAGGTC TGGGTCTTCA ACAAGAACTA CACGCAGTAC ATCCCTCTGT 
     1561 CCATATATGA CCTGCAGACG TGGATGGGCA GCCCGTCGAT CTTCGTCTAC GACTGCTCCA 
     1621 ATGCTGGCTT GATCGTCAAG TCCTTCAAGC AGTTCGCACT ACAGCGGGAG CAGGAGCTGG 
     1681 AGGTAGCTGC AATCAACCCA AATCACCCTC TTGCTCAGAT GCCTTTGCCT CCGTCGATGA 
     1741 AAAACTGCAT CCAGCTGGCA GCCTGCGAGG CCACCGAGCT GCTGCCCATG ATCCCCGACC 
     1801 TCCCGGCTGA CCTATTCACC TCCTGCCTCA CCACCCCCAT CAAGATCGCC CTGCGCTGGT 
     1861 TTTGCATGCA GAAATGTGTC AGTCTGGTGC CTGGCGTCAC ACTGGATTTG ATAGAAAAGA 
     1921 TCCCTGGCCG CCTGAACGAC AGGAGGACGC CCCTGGGTGA ACTGAACTGG ATCTTCACAG 
     1981 CCATCACAGA CACCATCGCG TGGAACGTGC TCCCCCGGGA TCTCTTCCAA AAGCTCTTCA 
     2041 GACAGGACTT GCTGGTGGCT AGTCTGTTTC GAAATTTTTT ATTGGCGGAA AGGATTATGA 
     2101 GGTCGTATAA CTGCACTCCC GTCAGCAGCC CGCGTCTGCC GCCCACGTAC ATGCACGCCA 
     2161 TGTGGCAAGC CTGGGACCTG GCTGTTGACA TCTGTCTGTC TCAGCTGCCG ACGATCATCG 
     2221 AGGAAGGCAC TGCGTTTCGG CACAGCCCGT TCTTCGCCGA GCAGCTGACC GCATTCCAGG 
     2281 TGTGGCTCAC CATGGGTGTG GAGAACCGAA ACCCACCCGA ACAGCTGCCC ATCGTCCTGC 
     2341 AGGTGCTGTT AAGCCAAGTG CACCGGCTGA GAGCATTGGA CTTGCTTGGA AGATTTTTGG 
     2401 ACCTGGGTCC CTGGGCAGTG AGCCTGGCCT TGTCTGTCGG CATCTTCCCC TACGTGCTGA 
     2461 AGCTGCTCCA GAGCTCGGCC CGAGAGCTGC GGCCACTTCT CGTTTTCATC TGGGCCAAGA 
     2521 TCCTCGCAGT GGACAGCTCG TGCCAAGCGG ACCTCGTGAA GGACAACGGC CACAAGTACT 
     2581 TCCTGTCGGT CCTGGCGGAC CCCTACATGC CAGCTGAACA CCGGACCATG ACGGCTTTCA 
     2641 TTCTCGCCGT GATCGTCAAC AGCTATCACA CGGGGCAGGA AGCCTGCCTT CAGGGAAACC 
     2701 TCATTGCCAT CTGCCTGGAG CAGCTCAACG ACCCGCACCC CTTGCTGCGC CAGTGGGTGG 
     2761 CCATCTGCCT CGGCAGGATC TGGCAGAACT TCGACTCGGC GAGGTGGTGC GGCGTGAGGG 
     2821 ACAGCGCTCA TGAGAAGCTC TACAGCCTCC TCTCCGACCC CATTCCCGAG GTCCGCTGCG 
     2881 CAGCGGTCTT CGCCCTTGGC ACGTTCGTGG GCAACTCTGC AGAGAGGACG GACCACTCCA 
     2941 CCACCATCGA CCACAACGTG GCCATGATGC TGGCCCAGCT GGTCAGCGAC GGGAGCCCCA 
     3001 TGGTCCGGAA GGAGCTGGTG GTGGCTCTGA GTCATCTTGT GGTTCAGTAT GAAAGCAATT 
     3061 TCTGCACCGT GGCCCTGCAG TTCATAGAAG AGGAAAAGAA CTACGCCTTG CCTTCTCCAG 
     3121 CAACCACAGA GGGAGGGAGT TTGACCCCAG TGCGAGACAG CCCGTGCACC CCCAGACTTC 
     3181 GTTCTGTGAG CTCCTATGGA AACATCCGTG CTGTCGCCAC AGCCAGGAGC CTCAACAAAT 
     3241 CTTTGCAGAA CCTGAGTTTG ACAGAGGAAT CTGGTGGCGC GGTGGCGTTC TCCCCCGGAA 
     3301 ACCTCAGCAC CAGCAGCAGC GCCAGCAGCA CCCTGGGCAG CCCCGAGAAT GAGGAGCATA 
     3361 TCCTGTCCTT CGAGACCATC GACAAGATGC GCCGCGCCAG CTCCTACTCC TCCCTCAACT 
     3421 CCCTCATCGG AGTTTCCTTT AACAGTGTTT ACACTCAGAT TTGGAGAGTC CTGCTGCACC 
     3481 TGGCTGCTGA CCCCTATCCA GAGGTCTCGG ACGTGGCCAT GAAAGTACTC AACAGCATCG 
     3541 CCTACAAGGC CACCGTGAAC GCCCGGCCGC AGCGCGTCCT GGACACCTCC TCCCTCACGC 
     3601 AGTCGGCCCC CGCCAGCCCC ACCAACAAGG GCGTGCACAT CCACCAGGCG GGGGGCTCCC 
     3661 CTCCGGCGTC CAGCACCAGC AGCTCCAGCC TGACCAACGA TGTGGCCAAG CAGCCGGTCA 
     3721 GCCGAGACTT GCCTTCTGGC CGGCCGGGCA CCACAGGCCC CGCTGGGGCG CAGTACACCC 
     3781 CTCACTCCCA CCAGTTCCCC CGGACACGGA AGATGTTCGA CAAGGGCCCA GAGCAGACTG 
     3841 CGGACGACGC GGACGATGCT GCTGGACACA AAAGTTTCAT CTCCGCCACG GTGCAGACGG 
     3901 GGTTCTGCGA CTGGAGCGCC CGCTATTTTG CCCAGCCCGT CATGAAGATC CCAGAAGAGC 
     3961 ACGACCTGGA GAGTCAGATC CGCAAGGAGC GGGAGTGGCG GTTCCTGCGA AACAGCCGTG 
     4021 TCAGGAGGCA GGCCCAGCAA GTCATTCAGA AGGGCATTAC GAGATTGGAC GACCAAATAT 
     4081 TTCTGAACAG GAACCCCGGC GTCCCCTCTG TGGTGAAATT CCACCCCTTC ACGCCGTGCA 
     4141 TCGCCGTAGC CGACAAGGAC AGCATCTGCT TTTGGGACTG GGAGAAAGGG GAGAAGCTGG 
     4201 ATTATTTCCA CAATGGGAAC CCTCGGTACA CGAGGGTCAC TGCCATGGAG TATCTGAACG 
     4261 GCCAGGACTG CTCGCTTCTG CTGACGGCCA CAGACGATGG TGCCATCAGG GTCTGGAAGA 
     4321 ATTTTGCTGA TTTGGAAAAG AACCCAGAGA TGGTGACCGC GTGGCAGGGG CTCTCGGACA 
     4381 TGCTGCCAAC GACGCGAGGA GCTGGGATGG TGGTGGACTG GGAGCAGGAG ACCGGCCTCC 
     4441 TCATGAGCTC AGGAGACGTG CGGATCGTCC GGATCTGGGA CACAGACCGT GAGATGAAGG 
     4501 TGCAGGACAT CCCTACGGGC GCAGACAGCT GTGTGACGAG TCTGTCCTGT GATTCCCACC 
     4561 GCTCACTCAT CGTGGCTGGC CTCGGTGACG GCTCCATCCG CGTCTACGAC AGAAGGATGG 
     4621 CACTCAGCGA ATGCCGTGTC ATGACGTACC GGGAGCACAC AGCCTGGGTG GTGAAGGCCT 
     4681 CCCTGCAGAA GCGTCCCGAC GGCCACATCG TGAGTGTGAG CGTCAATGGA GATGTGCGCA 
     4741 TCTTTGATCC CCGGATGCCT GAGTCGGTAA ATGTGCTTCA GATCGTGAAG GGGCTGACGG 
     4801 CCCTGGACAT CCACCCCCAG GCGGACCTGA TCGCATGTGG CTCCGTCAAT CAGTTCACCG 
     4861 CCATCTACAA CAGCAGCGGA GAGCTCATCA ACAACATCAA GTACTACGAC GGCTTCATGG 
     4921 GCCAGCGGGT CGGCGCCATC AGCTGCCTGG CCTTCCACCC GCACTGGCCT CACCTGGCCG 
     4981 TGGGAAGCAA CGACTACTAC ATCTCCGTGT ACTCGGTGGA GAAGCGTGTC AGAACGCGTA 
     5041 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     5101 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     5161 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     5221 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     5281 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     5341 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5401 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5461 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5521 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5581 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5641 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5701 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5761 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5821 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5881 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5941 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     6001 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     6061 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     6121 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     6181 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     6241 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     6301 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6361 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6421 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6481 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6541 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6601 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6661 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6721 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6781 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6841 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6901 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6961 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     7021 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     7081 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     7141 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     7201 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     7261 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     7321 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7381 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7441 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7501 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7561 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7621 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7681 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7741 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7801 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7861 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7921 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7981 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     8041 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     8101 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     8161 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     8221 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8281 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     8341 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8401 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8461 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8521 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8581 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8641 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8701 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8761 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8821 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8881 AATTTT
//