LOCUS       RC222277                9423 bp    DNA     circular  SYN 
DEFINITION  ARHGAP35 (Myc-DDK-tagged)-Human glucocorticoid receptor DNA binding factor 1 (GRLF1)
ACCESSION   NM_004491
VERSION     NM_004491.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5580..5671 
                     /label="Myc-DDK"  
     misc_feature    5701..6286 
                     /label="PolyA signal"  
     misc_feature    5758..5777 
                     /label="Restriction Enzyme"  
     misc_feature    6562..7234 
                     /label="ColE1"  
     misc_feature    7664..8458 
                     /label="Kan/Neo"  
     misc_feature    8493..8795 
                     /label="SV40 ori"  
     misc_feature    8881..9358 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5562..5568 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5562 
                     /label="ARHGAP35"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGATGGCA AGAAAGCAAG ATGTCCGAAT TCCCACCTAC AACATCAGTG 
     1081 TGGTGGGATT ATCTGGGACC GAGAAGGAAA AGGGCCAGTG TGGGATTGGA AAGTCTTGTT 
     1141 TGTGCAACCG CTTCGTGCGC CCGAGTGCTG ACGAGTTTCA CTTGGACCAT ACCTCCGTCC 
     1201 TCAGCACCAG TGACTTTGGA GGGCGAGTGG TCAATAATGA CCACTTTCTC TACTGGGGAG 
     1261 AAGTTAGCCG CTCCCTGGAG GATTGTGTGG AATGTAAGAT GCACATTGTG GAGCAGACTG 
     1321 AATTTATTGA TGATCAGACT TTTCAACCTC ATCGAAGCAC GGCCCTGCAG CCCTATATCA 
     1381 AGAGAGCTGC TGCGACCAAG CTTGCATCAG CTGAAAAACT CATGTACTTT TGCACTGACC 
     1441 AGCTGGGGCT GGAGCAGGAC TTTGAGCAGA AACAAATGCC AGACGGAAAG CTGCTGGTTG 
     1501 ATGGTTTTCT TCTTGGTATT GATGTTAGCA GGGGCATGAA TAGGAACTTT GATGACCAGC 
     1561 TCAAGTTTGT CTCCAATCTC TACAATCAGC TTGCAAAAAC AAAAAAGCCC ATAGTGGTGG 
     1621 TCCTGACTAA GTGTGACGAA GGTGTTGAGC GGTACATTAG AGATGCACAT ACTTTTGCCT 
     1681 TAAGCAAAAA GAACCTCCAG GTTGTGGAGA CCTCAGCGAG ATCCAATGTA AACGTGGACT 
     1741 TGGCTTTCAG CACCTTAGTG CAACTCATTG ATAAAAGTCC GGGGAAGACA AAAATCATTC 
     1801 CTTATTTTGA AGCTCTCAAG CAGCAGAGTC AGCAGATAGC TACAGCAAAA GACAAGTATG 
     1861 AGTGGCTGGT GAGTCGCATT GTGAAAAACC ACAATGAGAA CTGGCTGAGT GTCAGCCGAA 
     1921 AGATGCAGGC CTCTCCAGAA TACCAGGACT ATGACTACCT GGAAGGGACT CAGAAAGCCA 
     1981 AGAAGCTGTT TTTACAGCAC ATCCACCGCC TCAAGCATGA GCATATCGAG CGTAGGAGAA 
     2041 AGCTGTACCT GGCAGCCCTG CCATTAGCTT TTGAAGCTCT TATACCTAAT CTAGATGAAA 
     2101 TAGACCACCT AGGCTGCATA AAAGCCAAAA AGCTCTTAGA AACCAAGCCA GAATTCTTGA 
     2161 AGTGGTTTGT TGTGCTTGAA GAGACCCCAT GGGATGCCAC CAGTCACATT GACAACATGG 
     2221 AAAACGAACG GATTCCCTTT GATTTAATGG ATACCGTCCC TGCAGAGGCA CTATACGAGG 
     2281 CCCACTTAGA GAAGCTGAGG AACGAAAGGA AAAGAGTTGA GATGCGAAGG GCGTTTAAAG 
     2341 AAAACCTGGA GACTTCTCCT TTCATAACTC CCGGAAAGCC TTGGGAAGAG GCCCGTAGTT 
     2401 TTATTATGAA TGAGGATTTC TACCAGTGGC TGGAGGAATC TGTATACACG GATATTTATG 
     2461 GCAAACACCA AAAGCAAATT ATAGATAAAG CAAAGGAAGA ATTTCAGGAG TTGCTTTTGG 
     2521 AATATTCAGA ATTGTTTTAT GAACTGGAGC TGGATGCTAA GCCCAGCAAG GAGAAGATGG 
     2581 GTGTTATTCA GGATGTTCTG GGAGAGGAAC AGCGATTTAA AGCCATTTAC AAAAGCTCCA 
     2641 AGCAGAGCGT TGATGCCCTT ATTCTGAAAC ACATTCATTT TGTGTACCAC CCAACAAAGG 
     2701 AGACATGCCC CAGCTGCCCA GCTTGTGTGG ACGCTAAGAT TGAGCACTTG ATTAGTTCTC 
     2761 GGTTTATCCG GCCGTCTGAC CGGAATCAGA AAAATTCACT CTCTGACCCT AACATTGATA 
     2821 GAATCAACTT GGTTATATTG GGCAAAGACG CCTTGCCCGA GAGTTGGCCA ATGGAGATTA 
     2881 GAGCTCTTTG TACAAATGAT GACAAGTATG TGATAGATGG TAAAATGTAT GAGCTTTCCC 
     2941 TGAGGCCAAT AGAGGGGAAT GTCAGGCTTC CTGTGAACTC TTTCCAGACG CCAACATTTC 
     3001 AGCCCCACGG CTGTCTCTGC CTTTACAATT CAAAGGAATC GCTATCCTAT GTAGTGGAAA 
     3061 GTATAGAGAA GAGTAGAGAG TCCACGCTGG GCCGGCGGGA TAATCATTTA GTCCATCTCC 
     3121 CCCTTACATT AATTTTGGTT AACAAGAGAG GAGACACCAG TGGAGAGACT CTGCATAGCT 
     3181 TAATACAGCA AGGTCAACAA ATTGCTAGCA AACTTCAGTG TGTCTTTCTC GACCCTGCTT 
     3241 CTGCTGGCAT TGGTTACGGA CGCAACATTA ATGAAAAGCA AATCAGTCAA GTTTTGAAGG 
     3301 GACTCCTGGA CTCTAAGCGT AACTTAAACC TGGTCAGTTC TACTGCTAGC ATCAAAGATT 
     3361 TGGCTGATGT TGATCTGCGA ATTGTTATGT GTCTGATGTG TGGAGATCCT TTTAGTGCAG 
     3421 ATGATATACT TTTTCCTGTC CTTCAGTCCC AAACCTGTAA ATCTTCCCAT TGTGGAAGCA 
     3481 ACAACTCTGT TTTACTTGAA CTACCAATCG GACTGCACAA GAAGCGGATT GAACTGTCTG 
     3541 TTCTTTCATA CCATTCCTCC TTTAGCATCA GAAAGAGCCG GTTGGTTCAT GGGTACATTG 
     3601 TTTTTTATTC AGCCAAACGT AAGGCCTCTT TGGCTATGTT ACGTGCCTTT CTTTGTGAAG 
     3661 TGCAGGATAT TATCCCTATT CAGCTTGTAG CACTCACTGA TGGCGCTGTA GATGTCCTGG 
     3721 ACAATGACTT AAGTAGGGAA CAGCTAACTG AGGGGGAGGA GATTGCTCAA GAAATTGACG 
     3781 GAAGGTTCAC AAGCATCCCC TGTAGCCAAC CCCAGCATAA ACTTGAGATC TTTCACCCAT 
     3841 TTTTTAAAGA TGTGGTGGAA AAAAAGAACA TAATCGAGGC TACTCATATG TACGATAATG 
     3901 CTGCCGAGGC CTGTAGCACC ACCGAAGAGG TGTTTAACTC CCCCCGGGCA GGATCACCGC 
     3961 TCTCGAACTC AAACCTGCAG GATTCAGAAG AAGATATCGA GCCATCTTAC AGCCTGTTTC 
     4021 GAGAAGACAC ATCACTGCCT TCTCTGTCCA AAGACCATTC TAAGCTCTCT ATGGAACTGG 
     4081 AGGGAAATGA TGGGCTGTCT TTCATTATGA GCAATTTTGA GAGTAAACTG AACAACAAAG 
     4141 TACCTCCGCC AGTCAAACCA AAGCCTCCTG TCCATTTTGA AATTACAAAG GGGGATCTAT 
     4201 CTTATTTAGA CCAAGGCCAT AGGGATGGAC AGAGGAAGTC TGTGTCTTCT AGCCCCTGGC 
     4261 TGCCTCAGGA TGGGTTTGAT CCTTCTGACT ATGCTGAACC CATGGATGCT GTGGTGAAGC 
     4321 CAAGGAATGA AGAAGAAAAC ATATACTCCG TGCCCCATGA CAGCACCCAA GGCAAAATCA 
     4381 TCACCATTCG GAATATCAAC AAAGCCCAGT CCAACGGCAG CGGGAATGGT TCTGACAGTG 
     4441 AAATGGACAC CAGCTCTCTA GAGCGAGGGC GCAAGGTTTC CATCGTGAGC AAGCCAGTGC 
     4501 TGTACAGGAC GAGATGCACC CGGCTGGGGC GGTTTGCTAG TTACCGGACC AGCTTCAGCG 
     4561 TCGGGAGTGA TGATGAGCTG GGGCCCATCC GGAAGAAAGA GGAGGATCAG GCATCCCAGG 
     4621 GTTATAAAGG GGACAATGCT GTCATTCCAT ACGAAACAGA CGAAGACCCG CGGAGGAGGA 
     4681 ATATTCTTCG CAGCCTAAGG AGGAACACTA AGAAACCAAA GCCCAAACCC CGGCCATCCA 
     4741 TCACAAAGGC AACCTGGGAG AGTAACTATT TTGGGGTGCC CTTAACAACT GTCGTGACTC 
     4801 CAGAGAAGCC GATCCCCATT TTTATTGAAA GATGTATTGA GTACATTGAA GCCACAGGAC 
     4861 TGAGCACGGA AGGCATCTAC CGGGTCAGCG GGAACAAGTC TGAGATCGAG AGTCTGCAGA 
     4921 GACAGTTTGA TCAAGACCAC AACCTGGACC TGGCAGAGAA AGACTTTACG GTGAATACCG 
     4981 TGGCTGGTGC CATGAAGAGC TTTTTCTCAG AACTGCCTGA CCCCCTGGTC CCGTATAACA 
     5041 TGCAGATCGA CTTGGTGGAA GCACACAAAA TCAACGACCG GGAGCAGAAG TTGCATGCCC 
     5101 TTAAGGAGGT ATTAAAGAAA TTTCCAAAGG AAAACCACGA AGTCTTCAAG TATGTCATCT 
     5161 CTCACCTAAA CAAGGTCAGC CACAACAACA AGGTGAATCT CATGACCAGC GAGAACCTCT 
     5221 CCATCTGCTT CTGGCCCACC TTGATGAGAC CTGATTTCAG CACTATGGAC GCCCTCACAG 
     5281 CCACGCGCAC CTACCAGACA ATCATTGAAC TCTTTATCCA GCAGTGCCCC TTCTTCTTCT 
     5341 ACAATCGGCC CATCACCGAG CCCCCCGGCG CCAGGCCCAG CTCCCCCTCT GCCGTGGCTT 
     5401 CCACCGTCCC CTTCCTCACT TCCACGCCTG TCACAAGTCA GCCGTCGCCC CCACAGTCGC 
     5461 CTCCACCCAC CCCCCAGTCC CCAATGCAGC CACTGCTTCC CTCCCCGCTT CATCCCCACC 
     5521 GTCCCACTCC ACAGCCTTCC CGAAACATTC CCTGGCAAAC AAAGGAACAC ACGCGTACGC 
     5581 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     5641 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     5701 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     5761 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     5821 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     5881 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5941 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     6001 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     6061 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     6121 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     6181 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     6241 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     6301 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     6361 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     6421 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     6481 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     6541 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     6601 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     6661 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     6721 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     6781 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     6841 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     6901 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6961 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     7021 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     7081 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     7141 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     7201 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     7261 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     7321 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     7381 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     7441 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     7501 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     7561 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     7621 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     7681 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     7741 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     7801 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     7861 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     7921 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7981 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     8041 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     8101 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     8161 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     8221 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     8281 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     8341 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     8401 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     8461 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     8521 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     8581 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     8641 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     8701 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     8761 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     8821 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     8881 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     8941 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     9001 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     9061 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     9121 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     9181 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     9241 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     9301 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     9361 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     9421 TTT
//