LOCUS       RC222262                8148 bp    DNA     circular  SYN 
DEFINITION  PIGO (Myc-DDK-tagged)-Human phosphatidylinositol glycan anchor biosynthesis, class O (PIGO), transcript variant 1
ACCESSION   NM_032634
VERSION     NM_032634.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4305..4396 
                     /label="Myc-DDK"  
     misc_feature    4426..5011 
                     /label="PolyA signal"  
     misc_feature    4483..4502 
                     /label="Restriction Enzyme"  
     misc_feature    5287..5959 
                     /label="ColE1"  
     misc_feature    6389..7183 
                     /label="Kan/Neo"  
     misc_feature    7218..7520 
                     /label="SV40 ori"  
     misc_feature    7606..8083 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4287..4293 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4287 
                     /label="PIGO"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGAAAGCC TCAGTGTTGC TCTTCCTGGC CTGGGTCTGC TTCCTCTTCT 
     1081 ACGCTGGCAT TGCCCTCTTC ACCAGTGGCT TCCTGCTCAC CCGTTTGGAG CTCACCAACC 
     1141 ATAGCAGCTG CCAAGAGCCC CCAGGCCCTG GGTCCCTGCC ATGGGGGAGC CAAGGGAAAC 
     1201 CTGGGGCCTG CTGGATGGCT TCCCGATTTT CGCGGGTTGT GTTGGTGCTG ATAGATGCTC 
     1261 TGCGATTTGA CTTCGCCCAG CCCCAGCATT CACACGTGCC TAGAGAGCCT CCTGTCTCCC 
     1321 TACCCTTCCT GGGCAAACTA AGCTCCTTGC AGAGGATCCT GGAGATTCAG CCCCACCATG 
     1381 CCCGGCTCTA CCGATCTCAG GTTGACCCTC CTACCACCAC CATGCAGCGC CTCAAGGCCC 
     1441 TCACCACTGG CTCACTGCCT ACCTTTATTG ATGCTGGTAG TAACTTCGCC AGCCACGCCA 
     1501 TAGTGGAAGA CAATCTCATT AAGCAGCTCA CCAGTGCAGG AAGGCGTGTA GTCTTCATGG 
     1561 GAGATGATAC CTGGAAAGAC CTTTTCCCTG GTGCTTTCTC CAAAGCTTTC TTCTTCCCAT 
     1621 CCTTCAATGT CAGAGACCTA GACACAGTGG ACAATGGCAT CCTGGAACAC CTCTACCCCA 
     1681 CCATGGACAG TGGTGAATGG GACGTGCTGA TTGCTCACTT CCTGGGTGTG GACCACTGTG 
     1741 GCCACAAGCA TGGCCCTCAC CACCCTGAAA TGGCCAAGAA ACTTAGCCAG ATGGACCAGG 
     1801 TGATCCAGGG ACTTGTGGAG CGTCTGGAGA ATGACACACT GCTGGTAGTG GCTGGGGACC 
     1861 ATGGGATGAC CACAAATGGA GACCATGGAG GGGACAGTGA GCTGGAGGTC TCAGCTGCTC 
     1921 TCTTTCTGTA TAGCCCCACA GCAGTCTTCC CCAGCACCCC ACCAGAGGAG CCAGAGGTGA 
     1981 TTCCTCAAGT TAGCCTTGTG CCCACGCTGG CCCTGCTGCT GGGCCTGCCC ATCCCATTTG 
     2041 GGAATATCGG GGAAGTGATG GCTGAGCTAT TCTCAGGGGG TGAGGACTCC CAGCCCCACT 
     2101 CCTCTGCTTT AGCCCAAGCC TCAGCTCTCC ATCTCAATGC TCAGCAGGTG TCCCGATTTC 
     2161 TTCATACCTA CTCAGCTGCT ACTCAGGACC TTCAAGCTAA GGAGCTTCAT CAGCTGCAGA 
     2221 ACCTCTTCTC CAAGGCCTCT GCTGACTACC AGTGGCTTCT CCAGAGCCCC AAGGGGGCTG 
     2281 AGGCGACACT GCCGACTGTG ATTGCTGAGC TGCAGCAGTT CCTGCGGGGA GCTCGGGCCA 
     2341 TGTGCATCGA GTCTTGGGCT CGTTTCTCTC TGGTCCGCAT GGCGGGGGGT ACTGCTCTCT 
     2401 TGGCTGCTTC CTGCTTTATC TGCCTGCTGG CATCTCAGTG GGCAATATCC CCAGGCTTTC 
     2461 CATTCTGCCC TCTACTCCTG ACACCTGTGG CCTGGGGCCT GGTTGGGGCC ATAGCGTATG 
     2521 CTGGACTCCT GGGAACTATT GAGCTGAAGC TAGATCTAGT GCTTCTAGGG GCTGTGGCTG 
     2581 CAGTGAGCTC ATTCCTCCCT TTTCTGTGGA AAGCCTGGGC TGGCTGGGGG TCCAAGAGGC 
     2641 CCCTGGCAAC CCTGTTTCCC ATCCCTGGGC CCGTCCTGTT ACTCCTGCTG TTTCGCTTGG 
     2701 CTGTGTTCTT CTCTGATAGT TTTGTTGTAG CTGAGGCCAG GGCCACCCCC TTCCTTTTGG 
     2761 GCTCATTCAT CCTGCTCCTG GTTGTCCAGC TTCACTGGGA GGGCCAGCTG CTTCCACCTA 
     2821 AGCTACTCAC AATGCCCCGC CTTGGCACTT CAGCCACAAC AAACCCCCCA CGGCACAATG 
     2881 GTGCATATGC CCTGAGGCTT GGAATTGGGT TGCTTTTATG TACAAGGCTA GCTGGGCTTT 
     2941 TTCATCGTTG CCCTGAAGAG ACACCTGTTT GCCACTCCTC TCCCTGGCTG AGTCCTCTGG 
     3001 CATCCATGGT GGGTGGTCGA GCCAAGAATT TGTGGTATGG AGCTTGTGTG GCGGCGCTGG 
     3061 TGGCCCTGTT AGCTGCCGTG CGCTTGTGGC TTCGCCGCTA TGGTAATCTC AAGAGCCCCG 
     3121 AGCCACCCAT GCTCTTTGTG CGCTGGGGAC TGCCCCTAAT GGCATTGGGT ACTGCTGCCT 
     3181 ACTGGGCATT GGCGTCGGGG GCAGATGAGG CTCCCCCCCG TCTCCGGGTC CTGGTCTCTG 
     3241 GGGCATCCAT GGTGCTGCCT CGGGCTGTAG CAGGGCTGGC TGCTTCAGGG CTCGCGCTGC 
     3301 TGCTCTGGAA GCCTGTGACA GTGCTGGTGA AGGCTGGGGC AGGCGCTCCA AGGACCAGGA 
     3361 CTGTCCTCAC TCCCTTCTCA GGCCCCCCCA CTTCTCAAGC TGACTTGGAT TATGTGGTCC 
     3421 CTCAAATCTA CCGACACATG CAGGAGGAGT TCCGGGGCCG GTTAGAGAGG ACCAAATCTC 
     3481 AGGGTCCCCT GACTGTGGCT GCTTATCAGT TGGGGAGTGT CTACTCAGCT GCTATGGTCA 
     3541 CAGCCCTCAC CCTGTTGGCC TTCCCACTTC TGCTGTTGCA TGCGGAGCGC ATCAGCCTTG 
     3601 TGTTCCTGCT TCTGTTTCTG CAGAGCTTCC TTCTCCTACA TCTGCTTGCT GCTGGGATAC 
     3661 CCGTCACCAC CCCTGGTCCT TTTACTGTGC CATGGCAGGC AGTCTCGGCT TGGGCCCTCA 
     3721 TGGCCACACA GACCTTCTAC TCCACAGGCC ACCAGCCTGT CTTTCCAGCC ATCCATTGGC 
     3781 ATGCAGCCTT CGTGGGATTC CCAGAGGGTC ATGGCTCCTG TACTTGGCTG CCTGCTTTGC 
     3841 TAGTGGGAGC CAACACCTTT GCCTCCCACC TCCTCTTTGC AGTAGGTTGC CCACTGCTCC 
     3901 TGCTCTGGCC TTTCCTGTGT GAGAGTCAAG GGCTGCGGAA GAGACAGCAG CCCCCAGGGA 
     3961 ATGAAGCTGA TGCCAGAGTC AGACCCGAGG AGGAAGAGGA GCCACTGATG GAGATGCGGC 
     4021 TCCGGGATGC GCCTCAGCAC TTCTATGCAG CACTGCTGCA GCTGGGCCTC AAGTACCTCT 
     4081 TTATCCTTGG TATTCAGATT CTGGCCTGTG CCTTGGCAGC CTCCATCCTT CGCAGGCATC 
     4141 TCATGGTCTG GAAAGTGTTT GCCCCTAAGT TCATATTTGA GGCTGTGGGC TTCATTGTGA 
     4201 GCAGCGTGGG ACTTCTCCTG GGCATAGCTT TGGTGATGAG AGTGGATGGT GCTGTGAGCT 
     4261 CCTGGTTCAG GCAGCTATTT CTGGCCCAGC AGAGGACGCG TACGCGGCCG CTCGAGCAGA 
     4321 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4381 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4441 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4501 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4561 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4621 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4681 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4741 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4801 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4861 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4921 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4981 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5041 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5101 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5161 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5221 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5281 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5341 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5401 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5461 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5521 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5581 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5641 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5701 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5761 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5821 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     5881 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     5941 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6001 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6061 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6121 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6181 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6241 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6301 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6361 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6421 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6481 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6541 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6601 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6661 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6721 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6781 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6841 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     6901 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6961 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7021 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7081 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7141 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7201 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7261 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7321 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7381 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7441 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7501 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7561 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7621 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7681 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7741 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7801 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7861 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     7921 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7981 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8041 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8101 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//