LOCUS       RC222247                9090 bp    DNA     circular  SYN 
DEFINITION  UACA (Myc-DDK-tagged)-Human uveal autoantigen with coiled-coil domains and ankyrin repeats (UACA), transcript variant 2
ACCESSION   NM_001008224
VERSION     NM_001008224.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5247..5338 
                     /label="Myc-DDK"  
     misc_feature    5368..5953 
                     /label="PolyA signal"  
     misc_feature    5425..5444 
                     /label="Restriction Enzyme"  
     misc_feature    6229..6901 
                     /label="ColE1"  
     misc_feature    7331..8125 
                     /label="Kan/Neo"  
     misc_feature    8160..8462 
                     /label="SV40 ori"  
     misc_feature    8548..9025 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5229..5235 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5229 
                     /label="UACA"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGAACTGT TGGTTTTCTT GTACTCCTAA GAACAGACAT GCAGCAGATT 
     1081 GGAATAAATA TGATGACCGA TTGATGAAAG CAGCAGAAAG GGGGGATGTA GAAAAAGTGA 
     1141 CCTCAATCCT TGCTAAAAAG GGGGTCAATC CAGGCAAACT AGATGTGGAA GGCAGATCTG 
     1201 TCTTCCATGT TGTGACCTCA AAGGGGAATC TTGAGTGTTT GAATGCCATC CTTATACATG 
     1261 GAGTTGATAT TACAACCAGT GACACTGCAG GGAGAAATGC TCTTCACCTG GCTGCTAAGT 
     1321 ATGGACATGC ATTGTGCCTA CAAAAACTTC TACAGTACAA TTGTCCCACT GAGCATGCAG 
     1381 ACCTGCAGGG AAGAACTGCA CTTCACGATG CCGCAATGGC AGATTGTCCT TCTAGCATAC 
     1441 AGCTGCTTTG TGACCATGGG GCCTCTGTGA ATGCCAAAGA TGTAGACGGG CGGACACCAC 
     1501 TTGTTCTGGC TACTCAGATG AGTAGGCCAA CAATATGTCA ACTGCTGATA GATAGAGGAG 
     1561 CGGATGTTAA TTCCAGAGAC AAACAAAACA GAACTGCCCT CATGCTAGGT TGCGAATATG 
     1621 GTTGCAGAGA TGCAGTAGAA GTCTTAATTA AAAATGGTGC TGATATAAGC TTGCTGGATG 
     1681 CGCTTGGCCA TGATAGTTCT TACTATGCAA GAATTGGTGA CAATCTGGAC ATTCTAACCT 
     1741 TGTTGAAGAC TGCATCGGAA AATACCAACA AAGGGAGAGA ACTTTGGAAG AAAGGGCCAT 
     1801 CTTTGCAACA GCGAAATTTG ACACACATGC AAGATGAAGT AAATGTGAAG TCACATCAGA 
     1861 GGGAGCATCA AAATATTCAG GATTTGGAGA TTGAAAATGA AGATTTGAAA GAGAGGTTGA 
     1921 GAAAAATTCA GCAAGAACAA AGAATACTTT TGGATAAAGT CAATGGTTTA CAGTTACAGC 
     1981 TGAATGAGGA AGTTATGGTT GCTGATGATC TGGAAAGCGA GAGAGAAAAG CTGAAGTCCC 
     2041 TTTTGGCAGC TAAAGAAAAG CAACATGAAG AAAGCTTAAG GACTATTGAG GCTCTGAAAA 
     2101 ATAGATTTAA ATATTTTGAG AGTGATCATT TAGGATCAGG AAGTCATTTC AGTAACCGAA 
     2161 AAGAAGATAT GCTTCTTAAA CAAGGTCAGA TGTATATGGC AGACTCACAG TGTACTTCCC 
     2221 CAGGTATACC AGCCCATATG CAAAGCAGAT CTATGTTAAG ACCTCTGGAA CTATCTTTAC 
     2281 CCAGTCAAAC GTCATACTCT GAAAATGAAA TTTTAAAGAA AGAGTTAGAA GCAATGCGAA 
     2341 CTTTCTGTGA GTCAGCAAAA CAAGACCGAC TGAAGCTCCA AAATGAACTG GCACACAAAG 
     2401 TGGCAGAATG CAAAGCTTTA GCATTAGAAT GTGAAAGGGT CAAGGAGGAT TCAGATGAAC 
     2461 AGATAAAGCA ATTAGAAGAT GCATTAAAAG ATGTGCAGAA GAGGATGTAT GAGTCAGAAG 
     2521 GTAAAGTTAA ACAAATGCAG ACCCATTTTC TTGCCCTTAA AGAACACTTA ACAAGTGAAG 
     2581 CAGCCTCAGG GAATCACAGA CTAACCGAGG AACTGAAGGA TCAGTTGAAA GACTTGAAAG 
     2641 TAAAATATGA AGGTGCTTCA GCAGAAGTGG GGAAATTAAG AAACCAAATC AAACAAAATG 
     2701 AGATGATAGT AGAAGAGTTT AAGAGGGATG AAGGCAAGCT GATAGAAGAA AATAAGCGAT 
     2761 TACAGAAGGA ACTTAGTATG TGTGAAATGG AGCGAGAGAA GAAAGGAAGA AAGGTCACAG 
     2821 AGATGGAAGG CCAGGCAAAA GAATTGTCAG CGAAGTTGGC CCTTTCCATT CCAGCTGAAA 
     2881 AATTTGAAAA CATGAAGAGC TCATTATCAA ATGAAGTGAA TGAGAAAGCA AAAAAATTAG 
     2941 TAGAAATGGA AAGAGAACAT GAAAAATCAC TTAGTGAAAT TAGACAGTTA AAGAGAGAAC 
     3001 TTGAGAATGT TAAGGCCAAG CTTGCTCAGC ACGTCAAACC AGAGGAACAT GAACAGGTTA 
     3061 AGAGCAGATT AGAACAGAAA TCAGGAGAAC TTGGGAAGAA GATCACTGAG TTAACATTGA 
     3121 AAAATCAGAC ACTACAAAAG GAAATTGAAA AAGTTTATTT GGATAATAAG CTCCTCAAGG 
     3181 AGCAAGCACA TAACTTAACA ATTGAAATGA AAAATCATTA TGTTCCTTTA AAAGTAAGTG 
     3241 AAGACATGAA AAAGTCACAT GATGCAATTA TTGATGATCT TAATAGAAAG CTTTTAGATG 
     3301 TAACACAAAA ATATACAGAA AAGAAGTTGG AAATGGAGAA ATTGCTACTG GAAAATGACA 
     3361 GCTTAAGTAA GGATGTAAGC CGCCTAGAAA CTGTGTTTGT ACCTCCTGAG AAACATGAAA 
     3421 AAGAGATAAT AGCTCTGAAA TCCAATATTG TTGAACTTAA GAAACAGCTG TCTGAACTTA 
     3481 AGAAAAAATG TGGTGAAGAC CAGGAGAAAA TACACGCTCT CACATCTGAA AACACTAACT 
     3541 TGAAGAAGAT GATGAGTAAT CAGTATGTGC CAGTTAAAAC CCATGAAGAG GTTAAAATGA 
     3601 CACTGAATGA CACGTTAGCC AAAACTAACA GAGAATTATT AGATGTGAAG AAAAAATTTG 
     3661 AAGATATAAA TCAGGAATTT GTAAAAATAA AAGATAAGAA TGAAATATTA AAAAGAAACC 
     3721 TGGAAAACAC TCAGAACCAA ATAAAAGCTG AGTACATCAG CCTGGCAGAG CACGAGGCAA 
     3781 AGATGAGCTC GCTAAGTCAG AGCATGAGAA AGGTGCAGGA TAGTAATGCT GAAATCTTGG 
     3841 CCAACTACAG AAAAGGCCAA GAAGAGATTG TGACACTGCA TGCCGAAATT AAAGCCCAGA 
     3901 AGAAGGAGCT CGACACAATA CAAGAATGCA TTAAGGTAAA ATACGCCCCA ATTGTCAGCT 
     3961 TTGAGGAGTG CGAGAGAAAA TTTAAAGCAA CAGAGAAAGA ACTAAAAGAC CAGTTATCAG 
     4021 AGCAGACACA AAAGTATAGT GTCAGTGAAG AAGAAGTCAA GAAAAACAAG CAAGAGAATG 
     4081 ACAAGTTAAA GAAGGAGATT TTTACCCTTC AGAAAGATTT GAGAGATAAG ACAGTTCTCA 
     4141 TTGAGAAGTC TCATGAAATG GAAAGAGCAT TAAGCAGAAA AACAGACGAG CTAAACAAAC 
     4201 AGTTAAAAGA CTTGTCACAG AAATACACGG AAGTAAAGAA TGTGAAAGAG AAGCTAGTAG 
     4261 AAGAAAATGC CAAACAGACT TCTGAGATAC TTGCAGTGCA AAATCTTTTG CAAAAACAAC 
     4321 ATGTTCCATT GGAACAGGTT GAGGCTCTGA AAAAATCTCT TAATGGCACA ATTGAAAATC 
     4381 TAAAGGAAGA ACTGAAGAGT ATGCAAAGGT GTTACGAGAA AGAGCAGCAG ACAGTGACCA 
     4441 AACTGCATCA ATTGTTGGAG AATCAAAAGA ACTCTTCTGT ACCCCTGGCA GAGCATTTGC 
     4501 AGATTAAAGA AGCATTTGAG AAAGAAGTTG GAATCATAAA AGCCAGCTTG AGAGAAAAGG 
     4561 AAGAAGAAAG CCAAAACAAA ATGGAAGAAG TCTCCAAACT TCAGTCGGAG GTTCAGAATA 
     4621 CTAAACAAGC ATTAAAAAAA TTAGAGACTA GAGAGGTAGT TGACTTGTCT AAATATAAAG 
     4681 CAACAAAAAG TGATTTGGAG ACACAGATTT CTAGCTTAAA TGAAAAATTG GCCAATCTGA 
     4741 ATAGAAAGTA TGAGGAAGTA TGTGAGGAAG TTTTGCATGC CAAAAAGAAG GAAATATCTG 
     4801 CAAAAGATGA GAAGGAATTA CTGCATTTCA GCATTGAGCA AGAAATTAAG GATCAGAAGG 
     4861 AACGATGTGA TAAGTCCTTA ACAACAATCA CAGAGTTACA AAGAAGAATA CAAGAATCTG 
     4921 CTAAACAAAT AGAAGCAAAA GATAATAAGA TAACTGAACT GCTTAATGAT GTGGAAAGAT 
     4981 TAAAACAGGC ACTCAATGGC CTTTCCCAAC TCACCTACAC AAGTGGGAAC CCCACCAAGA 
     5041 GGCAGAGCCA GCTGATTGAC ACTCTGCAGC ACCAAGTGAA ATCTCTGGAG CAACAGCTGG 
     5101 CCGATGCTGA CAGACAGCAC CAAGAAGTAA TTGCAATTTA TCGGACACAC CTTCTTAGTG 
     5161 CTGCACAGGG TCACATGGAT GAAGATGTTC AGGAGGCTCT GCTCCAGATC ATACAAATGC 
     5221 GGCAGGGGCT TGTGTGCACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     5281 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     5341 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     5401 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5461 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5521 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5581 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5641 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5701 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5761 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5821 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5881 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5941 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     6001 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     6061 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     6121 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     6181 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     6241 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     6301 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     6361 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6421 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6481 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6541 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6601 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6661 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6721 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6781 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6841 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6901 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6961 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     7021 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     7081 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     7141 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     7201 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     7261 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     7321 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     7381 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7441 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7501 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7561 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7621 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7681 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7741 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7801 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7861 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7921 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7981 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     8041 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     8101 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     8161 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     8221 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     8281 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     8341 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8401 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8461 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8521 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8581 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8641 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8701 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8761 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8821 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8881 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8941 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     9001 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     9061 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//