LOCUS       RC222234L1                9273 bp    DNA     circular  SYN 
DEFINITION  NBR1 (Myc-DDK-tagged)-Human neighbor of BRCA1 gene 1 (NBR1), transcript variant 2
ACCESSION   NM_031858
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7564..7744 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8472..9131 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7903..8471 
                     /label="ColE1"  
     misc_feature    6439..6459 
                     /label="LR50 primer"  
     misc_feature    6236..6307 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6448..7039 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6218..6224 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6218 
                     /label="NBR1"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGAACCACAG GTTACTCTAA ATGTGACTTT 
     3361 TAAAAATGAA ATTCAAAGCT TTCTGGTTTC TGATCCAGAA AATACAACTT GGGCTGATAT 
     3421 CGAAGCTATG GTAAAAGTTT CATTTGATCT GAATACTATT CAAATAAAAT ACCTGGATGA 
     3481 GGAAAATGAA GAGGTATCCA TCAACAGTCA AGGAGAATAT GAAGAAGCGC TTAAGATGGC 
     3541 AGTTAAACAG GGAAACCAAC TGCAGATGCA AGTCCACGAA GGGCACCATG TCGTTGATGA 
     3601 AGCCCCACCC CCAGTTGTAG GAGCAAAACG ACTAGCTGCC AGGGCAGGGA AGAAGCCACT 
     3661 TGCACATTAC TCTTCACTGG TGAGAGTCTT GGGATCAGAC ATGAAGACCC CAGAGGATCC 
     3721 TGCAGTGCAG TCGTTTCCAC TTGTTCCATG TGACACAGAC CAGCCTCAAG ACAAGCCCCC 
     3781 AGACTGGTTC ACAAGCTACC TGGAGACGTT CAGAGAACAA GTGGTTAACG AAACGGTTGA 
     3841 GAAGCTTGAA CAGAAATTAC ATGAAAAGCT TGTCCTCCAG AACCCATCCT TGGGTTCTTG 
     3901 TCCCTCAGAA GTCTCAATGC CTACTTCAGA AGAAACATTG TTTTTGCCAG AAAACCAGTT 
     3961 CAGCTGGCAT ATTGCTTGCA ACAACTGCCA AAGAAGGATT GTTGGTGTCC GCTACCAGTG 
     4021 TAGCCTATGC CCATCCTACA ATATCTGTGA AGATTGTGAA GCAGGGCCAT ATGGCCATGA 
     4081 CACTAACCAC GTCCTGCTGA AGTTGCGGAG ACCTGTTGTG GGCTCCTCTG AACCGTTCTG 
     4141 TCACTCAAAG TACTCTACTC CTCGTCTTCC TGCTGCTCTG GAACAAGTCA GGCTCCAGAA 
     4201 ACAGGTTGAT AAGAACTTTC TTAAAGCAGA AAAGCAAAGG TTGCGAGCTG AGAAGAAACA 
     4261 ACGTAAAGCA GAGGTCAAGG AACTTAAAAA GCAGCTTAAA CTCCATAGGA AAATTCACCT 
     4321 GTGGAATTCA ATCCATGGAC TCCAGAGCCC CAAGTCTCCT TTAGGCCGAC CTGAGAGCTT 
     4381 GCTCCAGTCT AATACCCTGA TGCTCCCTTT GCAGCCCTGT ACCTCCGTTA TGCCAATGCT 
     4441 CAGTGCAGCA TTTGTGGATG AGAATTTGCC TGATGGGACT CACCTTCAGC CAGGAACCAA 
     4501 GTTTATCAAA CACTGGAGGA TGAAAAATAC AGGAAATGTA AAGTGGAGTG CAGACACAAA 
     4561 GCTCAAGTTC ATGTGGGGAA ACCTGACTTT GGCTTCCACA GAAAAGAAGG ATGTTTTGGT 
     4621 TCCCTGCCTC AAGGCCGGCC ATGTGGGAGT TGTATCTGTG GAGTTCATTG CCCCAGCCTT 
     4681 GGAGGGAACG TATACTTCCC ATTGGCGTCT TTCTCACAAA GGCCAGCAAT TTGGGCCTCG 
     4741 GGTCTGGTGC AGTATCATAG TAGATCCTTT CCCCTCCGAA GAGAGCCCTG ATAACATTGA 
     4801 AAAGGGCATG ATCAGCTCAA GCAAAACTGA TGATCTCACC TGCCAGCAAG AGGAAACTTT 
     4861 TCTTCTGGCT AAAGAAGAAA GACAGCTTGG TGAAGTGACT GAGCAGACAG AAGGGACAGC 
     4921 AGCCTGCATC CCACAGAAGG CAAAAAATGT TGCCAGTGAG AGGGAGCTCT ACATCCCATC 
     4981 TGTGGATCTT CTGACTGCCC AGGACCTGCT GTCCTTTGAG CTGTTGGATA TAAACATTGT 
     5041 TCAAGAGTTG GAGAGAGTGC CCCACAACAC CCCTGTGGAT GTGACTCCCT GCATGTCTCC 
     5101 TCTGCCACAT GACAGTCCTT TAATAGAGAA GCCAGGCTTG GGGCAGATAG AGGAAGAGAA 
     5161 TGAAGGGGCA GGATTTAAAG CACTTCCTGA TTCTATGGTG TCAGTAAAGA GGAAGGCTGA 
     5221 GAACATTGCT TCTGTGGAGG AAGCAGAAGA AGACCTGAGT GGGACCCAGT TTGTGTGTGA 
     5281 GACAGTAATC CGATCCCTTA CCTTGGATGC TGCCCCAGAC CACAACCCTC CTTGCAGACA 
     5341 GAAGTCCTTG CAGATGACAT TTGCCTTGCC TGAAGGACCA CTTGGAAATG AGAAGGAGGA 
     5401 GATTATCCAT ATCGCTGAGG AAGAAGCTGT CATGGAGGAG GAGGAGGATG AGGAGGATGA 
     5461 GGAGGAGGAG GATGAGCTCA AAGATGAAGT TCAAAGTCAG TCCTCTGCTT CCTCAGAGGA 
     5521 TTACATCATC ATCCTGCCTG AGTGCTTTGA TACCAGCCGC CCCCTGGGGG ATTCTATGTA 
     5581 CAGCTCTGCG CTCTCACAGC CAGGCCTGGA GCGAGGTGCT GAAGGCAAGC CTGGGGTTGA 
     5641 GGCTGGGCAG GAACCAGCTG AGGCTGGGGA AAGACTCCCT GGAGGGGAGA ACCAGCCACA 
     5701 GGAGCACAGC ATAAGTGACA TCCTCACGAC CTCACAGACT CTGGAAACAG TGCCCCTAAT 
     5761 CCCAGAGGTA GTGGAGCTTC CACCGTCACT GCCCAGGAGC TCTCCTTGTG TACATCATCA 
     5821 TGGTTCCCCA GGAGTGGATT TACCAGTTAC CATACCAGAA GTTTCTTCAG TCCCTGATCA 
     5881 GATCAGAGGA GAGCCCAGAG GCTCATCAGG ACTTGTAAAC AGCAGACAGA AGAGCTATGA 
     5941 CCACTCAAGG CACCATCATG GGAGCAGCAT TGCTGGAGGA CTGGTGAAGG GGGCTTTGTC 
     6001 TGTTGCTGCC TCTGCATACA AGGCCCTGTT TGCTGGGCCA CCAGTCACTG CACAGCCAAT 
     6061 AATTTCTGAA GATCAGACAG CAGCCCTGAT GGCCCGTCTC TTTGAAATGG GATTCTGTGA 
     6121 CAGGCAGCTG AACCTACGGC TGCTGAAGAA ACACAATTAC AATATCCTGC AGGTTGTGAC 
     6181 AGAACTTCTT CAGTTAAACA ACAACGACTG GTACAGCCAA CGCTATACGC GTACGCGGCC 
     6241 GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA 
     6301 GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCTG TACAAGTAGG ATTCGTCGAG 
     6361 GGACCTAATA ACTTCGTATA GCATACATTA TACGAAGTTA TACATGTTTA AGGGTTCCGG 
     6421 TTCCACTAGG TACAATTCGA TATCAAGCTT ATCGATAATC AACCTCTGGA TTACAAAATT 
     6481 TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT 
     6541 GCTTTAATGC CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG 
     6601 TATAAATCCT GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC 
     6661 GTGGTGTGCA CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT 
     6721 CAGCTCCTTT CCGGGACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC 
     6781 GCCTGCCTTG CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG 
     6841 TTGTCGGGGA AATCATCGTC CTTTCCTTGG CTGCTCGCCT GTGTTGCCAC CTGGATTCTG 
     6901 CGCGGGACGT CCTTCTGCTA CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC 
     6961 GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA GACGAGTCGG 
     7021 ATCTCCCTTT GGGCCGCCTC CCCGCATCGA TACCGTCGAC CTCGATCGAG ACCTAGAAAA 
     7081 ACATGGAGCA ATCACAAGTA GCAATACAGC AGCTACCAAT GCTGATTGTG CCTGGCTAGA 
     7141 AGCACAAGAG GAGGAGGAGG TGGGTTTTCC AGTCACACCT CAGGTACCTT TAAGACCAAT 
     7201 GACTTACAAG GCAGCTGTAG ATCTTAGCCA CTTTTTAAAA GAAAAGGGGG GACTGGAAGG 
     7261 GCTAATTCAC TCCCAACGAA GACAAGATAT CCTTGATCTG TGGATCTACC ACACACAAGG 
     7321 CTACTTCCCT GATTGGCAGA ACTACACACC AGGGCCAGGG ATCAGATATC CACTGACCTT 
     7381 TGGATGGTGC TACAAGCTAG TACCAGTTGA GCAAGAGAAG GTAGAAGAAG CCAATGAAGG 
     7441 AGAGAACACC CGCTTGTTAC ACCCTGTGAG CCTGCATGGG ATGGATGACC CGGAGAGAGA 
     7501 AGTATTAGAG TGGAGGTTTG ACAGCCGCCT AGCATTTCAT CACATGGCCC GAGAGCTGCA 
     7561 TCCGGACTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA 
     7621 ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG 
     7681 TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG 
     7741 GAAAATCTCT AGCAGCATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     7801 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     7861 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     7921 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     7981 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     8041 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     8101 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     8161 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     8221 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     8281 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     8341 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     8401 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     8461 TTAAGGGATT TTGGTCATGA TTACGCCCCG CCCTGCCACT CATCGCAGTA CTGTTGTAAT 
     8521 TCATTAAGCA TTCTGCCGAC ATGGAAGCCA TCACAAACGG CATGATGAAC CTGAATCGCC 
     8581 AGCGGCATCA GCACCTTGTC GCCTTGCGTA TAATATTTGC CCATGGTGAA AACGGGGGCG 
     8641 AAGAAGTTGT CCATATTGGC CACGTTTAAA TCAAAACTGG TGAAACTCAC CCAGGGATTG 
     8701 GCTGAGACGA AAAACATATT CTCAATAAAC CCTTTAGGGA AATAGGCCAG GTTTTCACCG 
     8761 TAACACGCCA CATCTTGCGA ATATATGTGT AGAAACTGCC GGAAATCGTC GTGGTATTCA 
     8821 CTCCAGAGCG ATGAAAACGT TTCAGTTTGC TCATGGAAAA CGGTGTAACA AGGGTGAACA 
     8881 CTATCCCATA TCACCAGCTC ACCGTCTTTC ATTGCCATAC GGAACTCCGG ATGAGCATTC 
     8941 ATCAGGCGGG CAAGAATGTG AATAAAGGCC GGATAAAACT TGTGCTTATT TTTCTTTACG 
     9001 GTCTTTAAAA AGGCCGTAAT ATCCAGCTGA ACGGTCTGGT TATAGGTACA TTGAGCAACT 
     9061 GACTGAAATG CCTCAAAATG TTCTTTACGA TGCCATTGGG ATATATCAAC GGTGGTATAT 
     9121 CCAGTGATTT TTTTCTCCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     9181 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTC 
     9241 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GAC
//