LOCUS       RC222229                8265 bp    DNA     circular  SYN 
DEFINITION  MUC4 (Myc-DDK-tagged)-Human mucin 4, cell surface associated (MUC4), transcript variant 5
ACCESSION   NM_138297
VERSION     NM_138297.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4420..4511 
                     /label="Myc-DDK"  
     misc_feature    4541..5126 
                     /label="PolyA signal"  
     misc_feature    4598..4617 
                     /label="Restriction Enzyme"  
     misc_feature    5402..6074 
                     /label="ColE1"  
     misc_feature    6504..7298 
                     /label="Kan/Neo"  
     misc_feature    7333..7635 
                     /label="SV40 ori"  
     misc_feature    7721..8198 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4395..4402 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4395 
                     /label="MUC4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGGGGGCA CGCTGGAGGA GGGTCCCCTG GGTGTCCCTG AGCTGCCTGT 
     1081 GTCTCTGCCT CCTTCCGCAT GTGGTCCCAG GAGTTTCCCT CTTCCCCTAT GGGGCAGGCG 
     1141 CCGGGGACCT GGAGTTCGTC AGGAGGACCG TGGACTTCAC CTCCCCACTC TTCAAGCCGG 
     1201 CGACTGGCTT CCCCCTTGGC TCCTCTCTCC GTGATTCCCT CTACTTCACA GACAATGGCC 
     1261 AGATCATCTT CCCAGAGTCA GACTACCAGA TTTTCTCCTA CCCCAACCCA CTCCCAACAG 
     1321 GCTTCACAGG CCGGGACCCT GTGGCCCTGG TGGCTCCGTT CTGGGACGAT GCTGACTTCT 
     1381 CCACTGGTCG GGGGACCACA TTTTATCAGG AATACGAGAC GTTCTATGGT GAACACAGCC 
     1441 TGCTAGTCCA GCAGGCCGAG TCTTGGATTA GAAAGATGAC AAACAACGGG GGCTACAAGG 
     1501 CCAGGTGGGC CCTAAAGGTC ACGTGGGTCA ATGCCCACGC CTATCCTGCC CAGTGGACCC 
     1561 TCGGGAGCAA CACCTACCAA GCCATCCTCT CCACGGACGG GAGCAGGTCC TATGCCCTGT 
     1621 TTCTCTACCA GAGCGGTGGG ATGCAGTGGG ACGTGGCCCA GCGCTCAGGC AACCCGGTGC 
     1681 TCATGGGCTT CTCTAGTGGA GATGGCTATT TCGAAAACAG CCCACTGATG TCCCAGCCAG 
     1741 TGTGGGAGAG GTATCGCCCT GATAGATTCC TGAATTCCAA CTCAGGCCTC CAAGGGCTGC 
     1801 AGTTCTACAG GCTACACCGG GAAGAAAGGC CCAACTACCG TCTCGAGTGC CTGCAGTGGC 
     1861 TGAAGAGCCA GCCTCGGTGG CCCAGCTGGG GCTGGAACCA GGTCTCCTGC CCTTGTTCCT 
     1921 GGCAGCAGGG ACGACGGGAC TTACGATTCC AACCCGTCAG CATAGGTCGC TGGGGCCTCG 
     1981 GCAGTAGGCA GCTGTGCAGC TTCACCTCTT GGCGAGGAGG CGTGTGCTGC AGCTACGGGC 
     2041 CCTGGGGAGA GTTTCGTGAA GGCTGGCACG TGCAGCGTCC TTGGCAGTTG GCCCAGGAAC 
     2101 TGGAGCCACA GAGCTGGTGC TGCCGCTGGA ATGACAAGCC CTACCTCTGT GCCCTGTACC 
     2161 AGCAGAGGCG GCCCCACGTG GGCTGTGCTA CATACAGGCC CCCACAGCCC GCCTGGATGT 
     2221 TCGGGGACCC CCACATCACC ACCTTGGATG GTGTCAGTTA CACCTTCAAT GGGCTGGGGG 
     2281 ACTTCCTGCT GGTCGGGGCC CAAGACGGGA ACTCCTCCTT CCTGCTTCAG GGCCGCACCG 
     2341 CCCAGACTGG CTCAGCCCAG GCCACCAACT TCATCGCCTT TGCGGCTCAG TACCGCTCCA 
     2401 GCAGCCTGGG CCCCGTCACG GTCCAATGGC TCCTTGAGCC TCACGACGCA ATCCGTGTCC 
     2461 TGCTGGATAA CCAGACTGTG ACATTTCAGC CTGACCATGA AGACGGCGGA GGCCAGGAGA 
     2521 CGTTCAACGC CACCGGAGTC CTCCTGAGCC GCAACGGCTC TGAGGTCTCG GCCAGCTTCG 
     2581 ACGGCTGGGC CACCGTCTCG GTGATCGCGC TCTCCAACAT CCTCCACGCC TCCGCCAGCC 
     2641 TCCCGCCCGA GTACCAGAAC CGCACGGAGG GGCTCCTGGG GGTCTGGAAT AACAATCCAG 
     2701 AGGACGACTT CAGGATGCCC AATGGCTCCA CCATTCCCCC AGGGAGCCCT GAGGAGATGC 
     2761 TTTTCCACTT TGGAATGACC TGGCAGATCA ACGGGACAGG CCTCCTTGGC AAGAGGAATG 
     2821 ACCAGCTGCC TTCCAACTTC ACCCCTGTTT TCTACTCACA ACTGCAAAAA AACAGCTCCT 
     2881 GGGCTGAACA TTTGATCTCC AACTGTGACG GAGATAGCTC ATGCATCTAT GACACCCTGG 
     2941 CCCTGCGCAA CGCAAGCATC GGACTTCACA CGAGGGAAGT CAGTAAAAAC TACGAGCAGG 
     3001 CGAACGCCAC CCTCAATCAG TACCCGCCCT CCATCAATGG TGGTCGTGTG ATTGAAGCCT 
     3061 ACAAGGGGCA GACCACGCTG ATTCAGTACA CCAGCAATGC TGAGGATGCC AACTTCACGC 
     3121 TCAGAGACAG CTGCACCGAC TTGGAGCTCT TTGAGAATGG GACGTTGCTG TGGACACCCA 
     3181 AGTCGCTGGA GCCATTCACT CTGGAGATTC TAGCAAGAAG TGCCAAGATT GGCTTGGCAT 
     3241 CTGCACTCCA GCCCAGGACT GTGGTCTGCC ATTGCAATGC AGAGAGCCAG TGTTTGTACA 
     3301 ATCAGACCAG CAGGGTGGGC AACTCCTCCC TGGAGGTGGC TGGCTGCAAG TGTGACGGGG 
     3361 GCACCTTCGG CCGCTACTGC GAGGGCTCCG AGGATGCCTG TGAGGAGCCG TGCTTCCCGA 
     3421 GTGTCCACTG CGTTCCTGGG AAGGGCTGCG AGGCCTGCCC TCCAAACCTG ACTGGGGATG 
     3481 GGCGGCACTG TGCGGCTCTG GGGAGCTCTT TCCTGTGTCA GAACCAGTCC TGCCCTGTGA 
     3541 ATTACTGCTA CAATCAAGGC CACTGCTACA TCTCCCAGAC TCTGGGCTGT CAGCCCATGT 
     3601 GCACCTGCCC CCCAGCCTTC ACTGACAGCC GCTGCTTCCT GGCTGGGAAC AACTTCAGTC 
     3661 CAACTGTCAA CCTAGAACTT CCCTTAAGAG TCATCCAGCT CTTGCTCAGT GAAGAGGAAA 
     3721 ATGCCTCCAT GGCAGAAGTC AACGCCTCGG TGGCATACAG ACTGGGGACC CTGGACATGC 
     3781 GGGCCTTTCT CCGCAACAGC CAAGTGGAAC GAATCGATTC TGCAGCACCG GCCTCGGGAA 
     3841 GCCCCATCCA ACACTGGATG GTCATCTCGG AGTTCCAGTA CCGCCCTCGG GGCCCGGTCA 
     3901 TTGACTTCCT GAACAACCAG CTGCTGGCCG CGGTGGTGGA GGCGTTCTTA TACCACGTTC 
     3961 CACGGAGGAG TGAGGAGCCC AGGAACGACG TGGTCTTCCA GCCCATCTCC GGGGAAGACG 
     4021 TGCGCGATGT GACAGCCCTG AACGTGAGCA CGCTGAAGGC TTACTTCAGA TGCGATGGCT 
     4081 ACAAGGGCTA CGACCTGGTC TACAGCCCCC AGAGCGGCTT CACCTGCGTG TCCCCGTGCA 
     4141 GTAGGGGCTA CTGTGACCAT GGAGGCCAGT GCCAGCACCT GCCCAGTGGG CCCCGCTGCA 
     4201 GCTGTGTGTC CTTCTCCATC TACACGGCCT GGGGCGAGCA CTGTGAGCAC CTGAGCATGA 
     4261 AACTCGACGC GTTCTTCGGC ATCTTCTTTG GGGCCCTGGG CGGCCTCTTG CTGCTGGGGG 
     4321 TCGGGACGTT CGTGGTCCTG CGCTTCTGGG GTTGCTCCGG GGCCAGGTTC TCCTATTTCC 
     4381 TGAACTCAGC TGAGGCCTTG CCTAGCGGAC CGACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4441 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4501 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4561 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4621 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4681 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4741 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4801 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4861 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4921 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4981 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5041 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5101 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5161 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5221 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5281 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5341 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5401 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5461 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5521 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5581 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5641 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5701 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5761 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5821 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5881 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5941 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6001 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6061 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6121 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6181 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6241 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6301 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6361 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6421 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6481 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6541 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6601 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6661 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6721 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6781 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6841 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6901 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6961 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7021 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7081 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7141 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7201 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7261 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7321 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7381 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7441 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7501 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7561 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7621 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7681 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7741 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7801 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7861 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7921 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7981 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8041 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8101 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8161 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8221 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
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