LOCUS       RC222178                7815 bp    DNA     circular  SYN 
DEFINITION  GTF2I (Myc-DDK-tagged)-Human general transcription factor IIi (GTF2I), transcript variant 2
ACCESSION   NM_033000
VERSION     NM_033000.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3972..4063 
                     /label="Myc-DDK"  
     misc_feature    4093..4678 
                     /label="PolyA signal"  
     misc_feature    4150..4169 
                     /label="Restriction Enzyme"  
     misc_feature    4954..5626 
                     /label="ColE1"  
     misc_feature    6056..6850 
                     /label="Kan/Neo"  
     misc_feature    6885..7187 
                     /label="SV40 ori"  
     misc_feature    7273..7750 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3954..3960 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3954 
                     /label="GTF2I"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCAAGTT GCAATGTCCA CCCTCCCCGT TGAAGATGAG GAGTCCTCGG 
     1081 AGAGCAGGAT GGTGGTGACA TTCCTCATGT CAGCTCTCGA GTCCATGTGT AAAGAACTGG 
     1141 CCAAGTCCAA AGCCGAAGTG GCCTGCATTG CAGTGTATGA AACAGACGTG TTTGTCGTCG 
     1201 GAACTGAAAG AGGACGTGCT TTTGTCAATA CCAGAAAGGA TTTTCAAAAA GATTTTGTAA 
     1261 AATATTGTGT TGAAGAAGAA GAAAAAGCTG CAGAGATGCA TAAAATGAAA TCTACAACCC 
     1321 AGGCAAATCG GATGAGTGTA GATGCTGTAG AAATTGAAAC ACTCAGAAAA ACAGTTGAGG 
     1381 ACTATTTCTG CTTTTGCTAT GGGAAAGCTT TAGGCAAATC CACAGTGGTA CCTGTACCAT 
     1441 ATGAGAAGAT GCTGCGAGAC CAGTCGGCTG TGGTAGTGCA GGGGCTTCCG GAAGGTGTTG 
     1501 CCTTTAAACA CCCCGAGAAC TATGATCTTG CAACCCTGAA ATGGATTTTG GAGAACAAAG 
     1561 CAGGGATTTC ATTCATCATT AAGAGACCTT TTTTAGAGCC AAAGAAGCAT GTAGGTGGTC 
     1621 GTGTGATGGT AACAGATGCT GACAGGTCAA TACTATCTCC AGGTGGAAGT TGTGGCCCCA 
     1681 TCAAAGTGAA AACTGAACCC ACAGAAGATT CTGGCATTTC CCTGGAAATG GCAGCTGTGA 
     1741 CAGTAAAGGA AGAATCAGAA GATCCTGATT ATTATCAATA TAACATTCAA GGAAGCCACC 
     1801 ATTCTTCAGA GGGCAATGAA GGCACAGAAA TGGAAGTACC AGCAGAAGAT TCTACTCAAC 
     1861 ATGTCCCTTC AGAAACAAGT GAGGACCCTG AAGTTGAGGT GACTATTGAA GATGATGATT 
     1921 ATTCTCCACC GTCTAAGAGA CCAAAGGCCA ATGAGCTACC GCAGCCACCA GTCCCGGAAC 
     1981 CCGCCAATGC TGGGAAGCGG AAAGTGAGGG AGTTCAACTT CGAGAAATGG AATGCTCGCA 
     2041 TCACTGATCT ACGTAAACAA GTTGAAGAAT TGTTTGAAAG GAAATATGCT CAAGCCATAA 
     2101 AAGCCAAAGG TCCGGTGACG ATCCCGTACC CTCTTTTCCA GTCTCATGTT GAAGATCTTT 
     2161 ATGTAGAAGG ACTTCCTGAA GGAATTCCTT TTAGAAGGCC ATCTACTTAC GGAATTCCTC 
     2221 GCCTGGAGAG GATATTACTT GCAAAGGAAA GGATTCGTTT TGTGATTAAG AAACATGAGC 
     2281 TTCTGAATTC AACACGTGAA GATTTACAGC TTGATAAGCC AGCTTCAGGA GTAAAGGAAG 
     2341 AATGGTATGC CAGAATCACT AAATTAAGAA AGATGGTGGA TCAGCTTTTC TGCAAAAAAT 
     2401 TTGCGGAAGC CTTGGGGAGC ACTGAAGCCA AGGCTGTACC GTACCAAAAA TTTGAGGCAC 
     2461 ACCCGAATGA TCTGTACGTG GAAGGACTGC CAGAAAACAT TCCTTTCCGA AGTCCCTCAT 
     2521 GGTATGGAAT CCCAAGGCTG GAAAAAATCA TTCAAGTGGG CAATCGAATT AAATTTGTTA 
     2581 TTAAAAGACC AGAACTTCTG ACTCACAGTA CCACTGAAGT TACTCAGCCA AGAACGAATA 
     2641 CACCAGTCAA AGAAGATTGG AATGTCAGAA TTACCAAGCT ACGGAAGCAA GTGGAAGAGA 
     2701 TTTTTAATTT GAAATTTGCT CAAGCTCTTG GACTCACCGA GGCAGTAAAA GTACCATATC 
     2761 CTGTGTTTGA ATCAAACCCG GAGTTCTTGT ATGTGGAAGG CTTGCCAGAG GGGATTCCCT 
     2821 TCCGAAGCCC TACCTGGTTT GGAATTCCAC GACTTGAAAG GATCGTCCGC GGGAGTAATA 
     2881 AAATCAAGTT CGTTGTTAAA AAACCTGAAC TAGTTATTTC CTACTTGCCT CCTGGGATGG 
     2941 CTAGTAAAAT AAACACTAAA GCTTTGCAGT CCCCCAAAAG ACCACGAAGT CCTGGGAGTA 
     3001 ATTCAAAGGT TCCTGAAATT GAGGTCACCG TGGAAGGCCC TAATAACAAC AATCCTCAAA 
     3061 CCTCAGCTGT TCGAACCCCG ACCCAGACTA ACGGTTCTAA CGTTCCCTTC AAGCCACGAG 
     3121 GGAGAGAGTT TTCCTTTGAG GCCTGGAATG CCAAAATCAC GGACCTAAAA CAGAAAGTTG 
     3181 AAAATCTCTT CAATGAGAAA TGTGGGGAAG CTCTTGGCCT TAAACAAGCT GTGAAGGTGC 
     3241 CGTTCGCGTT ATTTGAGTCT TTCCCGGAAG ACTTTTATGT GGAAGGCTTA CCTGAGGGTG 
     3301 TGCCATTCCG AAGACCATCG ACTTTTGGCA TTCCGAGGCT GGAGAAGATA CTCAGAAACA 
     3361 AAGCCAAAAT TAAGTTCATC ATTAAAAAGC CCGAAATGTT TGAGACGGCG ATTAAGGAGA 
     3421 GCACCTCCTC TAAGAGCCCT CCCAGAAAAA TAAATTCATC ACCCAATGTT AATACTACTG 
     3481 CATCAGGTGT TGAAGACCTT AACATCATTC AGGTGACAAT TCCAGATGAT GATAATGAAA 
     3541 GACTCTCGAA AGTTGAAAAA GCTAGACAGC TAAGAGAACA AGTGAATGAC CTCTTTAGTC 
     3601 GGAAATTTGG TGAAGCTATT GGTATGGGTT TTCCTGTGAA AGTTCCCTAC AGGAAAATCA 
     3661 CAATTAACCC TGGCTGTGTG GTGGTTGATG GCATGCCCCC GGGGGTGTCC TTCAAAGCCC 
     3721 CCAGCTACCT GGAAATCAGC TCCATGAGAA GGATCTTAGA CTCTGCCGAG TTTATCAAAT 
     3781 TCACGGTCAT TAGACCATTT CCAGGACTTG TGATTAATAA CCAGCTGGTT GATCAGAGTG 
     3841 AGTCAGAAGG CCCCGTGATA CAAGAATCAG CTGAACCAAG CCAGTTGGAA GTTCCAGCCA 
     3901 CAGAAGAAAT AAAAGAGACT GATGGAAGCT CTCAGATCAA GCAAGAACCA GACCCCACGT 
     3961 GGACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4021 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4081 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4141 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4201 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4261 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4321 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4381 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4441 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4501 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4561 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4621 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     4681 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     4741 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     4801 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     4861 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     4921 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     4981 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5041 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5101 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5161 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5221 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5281 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5341 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5401 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5461 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     5521 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     5581 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     5641 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     5701 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     5761 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     5821 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     5881 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     5941 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6001 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6061 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6121 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6181 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6241 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6301 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6361 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6421 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6481 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6541 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     6601 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     6661 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     6721 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     6781 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     6841 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     6901 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     6961 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7021 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7081 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7141 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7201 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7261 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7321 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7381 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7441 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7501 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7561 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     7621 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     7681 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     7741 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     7801 TTTAACGCGA ATTTT
//