LOCUS       RC222138                8640 bp    DNA     circular  SYN 
DEFINITION  CYFIP2 (Myc-DDK-tagged)-Human cytoplasmic FMR1 interacting protein 2 (CYFIP2), transcript variant 1
ACCESSION   NM_001037333
VERSION     NM_001037333.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4797..4888 
                     /label="Myc-DDK"  
     misc_feature    4918..5503 
                     /label="PolyA signal"  
     misc_feature    4975..4994 
                     /label="Restriction Enzyme"  
     misc_feature    5779..6451 
                     /label="ColE1"  
     misc_feature    6881..7675 
                     /label="Kan/Neo"  
     misc_feature    7710..8012 
                     /label="SV40 ori"  
     misc_feature    8098..8575 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4779..4785 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4779 
                     /label="CYFIP2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACCACGCAC GTCACCCTGG AAGATGCCCT GTCCAACGTG GACCTGCTTG 
     1081 AAGAGCTTCC CCTCCCCGAC CAGCAGCCAT GCATCGAGCC TCCACCTTCC TCCATCATGT 
     1141 ACCAGGCTAA CTTTGACACA AACTTTGAGG ACAGGAATGC ATTTGTCACG GGCATTGCAA 
     1201 GGTACATTGA GCAGGCTACA GTCCACTCCA GCATGAATGA GATGCTGGAG GAAGGACATG 
     1261 AGTATGCGGT CATGCTGTAC ACCTGGCGCA GCTGTTCCCG GGCCATTCCC CAGGTGAAAT 
     1321 GCAACGAGCA GCCCAACCGA GTAGAGATCT ATGAGAAGAC AGTAGAGGTG CTGGAGCCGG 
     1381 AGGTCACCAA GCTCATGAAG TTCATGTATT TTCAGCGCAA GGCCATCGAG CGGTTCTGCA 
     1441 GCGAGGTGAA GCGGCTGTGC CATGCCGAGC GCAGGAAGGA CTTTGTCTCT GAGGCCTACC 
     1501 TCCTGACCCT TGGCAAGTTC ATCAACATGT TTGCTGTCCT GGATGAGCTA AAGAACATGA 
     1561 AGTGCAGCGT CAAGAATGAC CACTCTGCCT ACAAGAGGGC AGCACAGTTC CTGCGGAAGA 
     1621 TGGCAGATCC CCAGTCTATC CAGGAGTCGC AGAACCTTTC CATGTTCCTG GCCAACCACA 
     1681 ACAGGATCAC CCAGTGTCTC CACCAGCAAC TTGAAGTGAT CCCAGGCTAT GAGGAGCTGC 
     1741 TGGCTGACAT TGTCAACATC TGTGTGGATT ACTACGAGAA CAAGATGTAC CTGACTCCCA 
     1801 GTGAGAAACA TATGCTCCTC AAGGTGATGG GCTTTGGCCT CTACCTAATG GATGGAAATG 
     1861 TCAGTAACAT TTACAAACTG GATGCCAAGA AGAGAATTAA TCTTAGCAAA ATTGATAAAT 
     1921 TCTTTAAGCA GCTGCAGGTG GTGCCCCTTT TCGGCGACAT GCAGATAGAG CTGGCCAGAT 
     1981 ACATTAAGAC CAGTGCTCAC TATGAAGAGA ACAAGTCCAA GTGGACGTGC ACCCAGAGCA 
     2041 GCATCAGCCC CCAGTACAAT ATCTGCGAGC AGATGGTTCA GATCCGGGAT GACCACATCC 
     2101 GCTTCATCTC CGAGCTCGCT CGCTACAGCA ACAGTGAGGT GGTGACGGGC TCAGGGCTGG 
     2161 ACAGCCAGAA GTCAGACGAG GAGTATCGCG AGCTCTTCGA CCTAGCCCTG CGGGGTCTGC 
     2221 AGCTTCTATC CAAGTGGAGC GCCCACGTCA TGGAGGTGTA CTCTTGGAAG CTGGTTCATC 
     2281 CCACAGACAA GTTCTGCAAC AAGGACTGTC CTGGCACCGC GGAGGAATAT GAGAGAGCCA 
     2341 CACGCTACAA TTACACCAGT GAGGAAAAAT TTGCCTTCGT TGAGGTGATC GCCATGATCA 
     2401 AAGGCCTGCA GGTGCTCATG GGCAGGATGG AGAGCGTCTT CAACCAGGCC ATCAGGAACA 
     2461 CCATCTACGC GGCATTGCAG GACTTCGCCC AGGTGACGCT GCGTGAGCCC CTGCGGCAGG 
     2521 CGGTACGGAA GAAGAAGAAT GTCCTCATCA GCGTCCTACA GGCAATTCGA AAGACCATCT 
     2581 GTGACTGGGA GGGAGGGCGA GAGCCCCCTA ATGACCCATG CTTGAGAGGG GAGAAGGACC 
     2641 CCAAAGGTGG ATTTGATATC AAGGTGCCCC GGCGTGCTGT GGGGCCATCC AGCACACAGC 
     2701 TGTACATGGT GCGGACCATG CTTGAATCAC TCATTGCAGA CAAAAGCGGC TCCAAGAAGA 
     2761 CCCTGAGGAG CAGCCTGGAT GGACCCATTG TCCTCGCCAT AGAGGACTTT CACAAACAGT 
     2821 CCTTCTTCTT CACACATCTG CTCAACATCA GTGAAGCCCT GCAGCAGTGT TGTGACCTCT 
     2881 CCCAGCTCTG GTTCCGAGAA TTCTTCCTGG AGTTAACCAT GGGCCGACGA ATCCAGTTCC 
     2941 CCATCGAGAT GTCCATGCCC TGGATTCTAA CGGACCATAT CCTGGAAACC AAAGAACCTT 
     3001 CCATGATGGA GTATGTCCTC TACCCTCTGG ATCTGTACAA CGACAGCGCC TACTATGCTC 
     3061 TGACCAAGTT TAAAAAGCAG TTCCTGTACG ATGAGATAGA AGCTGAGGTG AACCTGTGTT 
     3121 TTGATCAGTT TGTCTACAAG CTGGCAGACC AGATCTTTGC TTACTACAAA GCCATGGCTG 
     3181 GCAGTGTCCT GTTGGATAAA CGTTTTCGAG CTGAGTGTAA GAATTATGGC GTCATCATTC 
     3241 CGTATCCACC GTCCAATCGC TATGAAACAC TGCTGAAGCA GAGACACGTC CAGCTGTTGG 
     3301 GTAGATCAAT TGACTTGAAC AGACTCATTA CCCAGCGCAC CTCTGCCGCC ATGTATAAAT 
     3361 CCTTGGACCA AGCTATCAGC CGCTTTGAGA GTGAGGACCT GACCTCCATT GTGGAGCTGG 
     3421 AGTGGCTGCT GGAGATTAAC CGGCTCACGC ATCGGCTGCT CTGTAAGCAT ATGACGCTGG 
     3481 ACAGCTTCGA TGCCATGTTC CGAGAGGCCA ATCACAATGT GTCCGCCCCC TATGGCCGTA 
     3541 TCACCCTGCA TGTCTTCTGG GAACTGAACT TTGACTTTCT CCCCAACTAC TGCTACAATG 
     3601 GGTCCACTAA CCGTTTTGTG CGGACTGCCA TTCCTTTCAC CCAAGAACCA CAACGAGACA 
     3661 AACCTGCCAA CGTCCAGCCT TATTACCTCT ATGGATCCAA GCCTCTCAAC ATTGCCTACA 
     3721 GCCACATCTA CAGCTCCTAC AGGAATTTCG TGGGGCCACC TCATTTCAAG ACTATCTGCA 
     3781 GACTCCTGGG TTATCAGGGC ATCGCTGTGG TCATGGAGGA ACTGCTAAAG ATTGTGAAGA 
     3841 GCTTGCTCCA AGGAACCATT CTCCAGTATG TGAAAACACT GATAGAGGTG ATGCCCAAGA 
     3901 TATGCCGCTT GCCCCGACAT GAGTATGGCT CCCCAGGGAT CCTGGAGTTC TTCCACCACC 
     3961 AGCTGAAGGA CATCATTGAG TACGCAGAGC TCAAAACAGA CGTGTTCCAG AGCCTGAGGG 
     4021 AAGTGGGCAA TGCCATCCTC TTCTGCCTCC TCATAGAGCA AGCTCTGTCT CAGGAGGAGG 
     4081 TCTGCGATTT GCTCCATGCC GCACCCTTCC AAAACATCTT GCCTAGAGTC TACATCAAAG 
     4141 AGGGGGAGCG CCTGGAGGTC CGGATGAAAC GTCTGGAAGC CAAGTATGCC CCGCTCCACC 
     4201 TGGTCCCTCT GATCGAGCGG CTGGGGACCC CTCAGCAAAT CGCCATTGCT CGCGAGGGTG 
     4261 ACCTCCTGAC CAAGGAGCGG CTGTGCTGTG GCCTGTCCAT GTTCGAGGTC ATCCTGACCC 
     4321 GCATTCGGAG CTACCTGCAG GACCCCATCT GGCGGGGCCC ACCGCCCACC AATGGCGTCA 
     4381 TGCACGTCGA TGAGTGTGTG GAGTTCCACC GGCTGTGGAG CGCCATGCAG TTCGTGTACT 
     4441 GCATCCCTGT GGGAACCAAC GAGTTCACAG CTGAGCAGTG TTTCGGCGAT GGCTTGAACT 
     4501 GGGCTGGTTG CTCCATCATT GTCCTGCTGG GCCAGCAGCG TCGCTTTGAC CTGTTCGACT 
     4561 TCTGTTACCA CCTGCTAAAA GTGCAGAGGC AGGACGGGAA GGATGAAATC ATTAAGAATG 
     4621 TGCCCCTGAA GAAGATGGCC GACCGGATCA GGAAGTATCA GATCTTGAAC AATGAGGTTT 
     4681 TTGCCATCCT GAACAAATAC ATGAAGTCCG TGGAGACAGA CAGTTCCACT GTGGAGCATG 
     4741 TGCGCTGCTT CCAGCCACCC ATCCACCAGT CCTTGGCCAC CACTTGCACG CGTACGCGGC 
     4801 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4861 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4921 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4981 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     5041 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     5101 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     5161 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     5221 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5281 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5341 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5401 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5461 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5521 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5581 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5641 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5701 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5761 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5821 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5881 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5941 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     6001 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     6061 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     6121 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     6181 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6241 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6301 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6361 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6421 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6481 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6541 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6601 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6661 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6721 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6781 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6841 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6901 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6961 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     7021 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     7081 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     7141 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     7201 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7261 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7321 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7381 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7441 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7501 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7561 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7621 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7681 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7741 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7801 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7861 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7921 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7981 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     8041 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     8101 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     8161 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     8221 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     8281 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8341 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8401 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8461 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8521 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8581 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//