LOCUS       RC222096                7413 bp    DNA     circular  SYN 
DEFINITION  KCNQ2 (Myc-DDK-tagged)-Human potassium voltage-gated channel, KQT-like subfamily, member 2 (KCNQ2), transcript variant 3
ACCESSION   NM_004518
VERSION     NM_004518.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3570..3661 
                     /label="Myc-DDK"  
     misc_feature    3691..4276 
                     /label="PolyA signal"  
     misc_feature    3748..3767 
                     /label="Restriction Enzyme"  
     misc_feature    4552..5224 
                     /label="ColE1"  
     misc_feature    5654..6448 
                     /label="Kan/Neo"  
     misc_feature    6483..6785 
                     /label="SV40 ori"  
     misc_feature    6871..7348 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3552..3558 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3552 
                     /label="KCNQ2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGCAGAAG TCGCGCAACG GCGGCGTATA CCCCGGCCCG AGCGGGGAGA 
     1081 AGAAGCTGAA GGTGGGCTTC GTGGGGCTGG ACCCCGGCGC GCCCGACTCC ACCCGGGACG 
     1141 GGGCGCTGCT GATCGCCGGC TCCGAGGCCC CCAAGCGCGG CAGCATCCTC AGCAAACCTC 
     1201 GCGCGGGCGG CGCGGGCGCC GGGAAGCCCC CCAAGCGCAA CGCCTTCTAC CGCAAGCTGC 
     1261 AGAATTTCCT CTACAACGTG CTGGAGCGGC CGCGCGGCTG GGCGTTCATC TACCACGCCT 
     1321 ACGTGTTCCT CCTGGTTTTC TCCTGCCTCG TGCTGTCTGT GTTTTCCACC ATCAAGGAGT 
     1381 ATGAGAAGAG CTCGGAGGGG GCCCTCTACA TCCTGGAAAT CGTGACTATC GTGGTGTTTG 
     1441 GCGTGGAGTA CTTCGTGCGG ATCTGGGCCG CAGGCTGCTG CTGCCGGTAC CGTGGCTGGA 
     1501 GGGGGCGGCT CAAGTTTGCC CGGAAACCGT TCTGTGTGAT TGACATCATG GTGCTCATCG 
     1561 CCTCCATTGC GGTGCTGGCC GCCGGCTCCC AGGGCAACGT CTTTGCCACA TCTGCGCTCC 
     1621 GGAGCCTGCG CTTCCTGCAG ATTCTGCGGA TGATCCGCAT GGACCGGCGG GGAGGCACCT 
     1681 GGAAGCTGCT GGGCTCTGTG GTCTATGCCC ACAGCAAGGA GCTGGTCACT GCCTGGTACA 
     1741 TCGGCTTCCT TTGTCTCATC CTGGCCTCGT TCCTGGTGTA CTTGGCAGAG AAGGGGGAGA 
     1801 ACGACCACTT TGACACCTAC GCGGATGCAC TCTGGTGGGG CCTGATCACG CTGACCACCA 
     1861 TTGGCTACGG GGACAAGTAC CCCCAGACCT GGAACGGCAG GCTCCTTGCG GCAACCTTCA 
     1921 CCCTCATCGG TGTCTCCTTC TTCGCGCTGC CTGCAGGCAT CTTGGGGTCT GGGTTTGCCC 
     1981 TGAAGGTTCA GGAGCAGCAC AGGCAGAAGC ACTTTGAGAA GAGGCGGAAC CCGGCAGCAG 
     2041 GCCTGATCCA GTCGGCCTGG AGATTCTACG CCACCAACCT CTCGCGCACA GACCTGCACT 
     2101 CCACGTGGCA GTACTACGAG CGAACGGTCA CCGTGCCCAT GTACAGACTT ATCCCCCCGC 
     2161 TGAACCAGCT GGAGCTGCTG AGGAACCTCA AGAGTAAATC TGGACTCGCT TTCAGGAAGG 
     2221 ACCCCCCGCC GGAGCCGTCT CCAAGCCAGA AGGTCAGTTT GAAAGATCGT GTCTTCTCCA 
     2281 GCCCCCGAGG CGTGGCTGCC AAGGGGAAGG GGTCCCCGCA GGCCCAGACT GTGAGGCGGT 
     2341 CACCCAGCGC CGACCAGAGC CTCGAGGACA GCCCCAGCAA GGTGCCCAAG AGCTGGAGCT 
     2401 TCGGGGACCG CAGCCGGGCA CGCCAGGCTT TCCGCATCAA GGGTGCCGCG TCACGGCAGA 
     2461 ACTCAGAAGA AGCAAGCCTC CCCGGAGAGG ACATTGTGGA TGACAAGAGC TGCCCCTGCG 
     2521 AGTTTGTGAC CGAGGACCTG ACCCCGGGCC TCAAAGTCAG CATCAGAGCC GTGTGTGTCA 
     2581 TGCGGTTCCT GGTGTCCAAG CGGAAGTTCA AGGAGAGCCT GCGGCCCTAC GACGTGATGG 
     2641 ACGTCATCGA GCAGTACTCA GCCGGCCACC TGGACATGCT GTCCCGAATT AAGAGCCTGC 
     2701 AGTCCAGAGT GGACCAGATC GTGGGGCGGG GCCCAGCGAT CACGGACAAG GACCGCACCA 
     2761 AGGGCCCGGC CGAGGCGGAG CTGCCCGAGG ACCCCAGCAT GATGGGACGG CTCGGGAAGG 
     2821 TGGAGAAGCA GGTCTTGTCC ATGGAGAAGA AGCTGGACTT CCTGGTGAAT ATCTACATGC 
     2881 AGCGGATGGG CATCCCCCCG ACAGAGACCG AGGCCTACTT TGGGGCCAAA GAGCCGGAGC 
     2941 CGGCGCCGCC GTACCACAGC CCGGAAGACA GCCGGGAGCA TGTCGACAGG CACGGCTGCA 
     3001 TTGTCAAGAT CGTGCGCTCC AGCAGCTCCA CGGGCCAGAA GAACTTCTCG GCGCCCCCGG 
     3061 CCGCGCCCCC TGTCCAGTGT CCGCCCTCCA CCTCCTGGCA GCCACAGAGC CACCCGCGCC 
     3121 AGGGCCACGG CACCTCCCCC GTGGGGGACC ACGGCTCCCT GGTGCGCATC CCGCCGCCGC 
     3181 CTGCCCACGA GCGGTCGCTG TCCGCCTACG GCGGGGGCAA CCGCGCCAGC ATGGAGTTCC 
     3241 TGCGGCAGGA GGACACCCCG GGCTGCAGGC CCCCCGAGGG GAACCTGCGG GACAGCGACA 
     3301 CGTCCATCTC CATCCCGTCC GTGGACCACG AGGAGCTGGA GCGTTCCTTC AGCGGCTTCA 
     3361 GCATCTCCCA GTCCAAGGAG AACCTGGATG CTCTCAACAG CTGCTACGCG GCCGTGGCGC 
     3421 CTTGTGCCAA AGTCAGGCCC TACATTGCGG AGGGAGAGTC AGACACCGAC TCCGACCTCT 
     3481 GTACCCCGTG CGGGCCCCCG CCACGCTCGG CCACCGGCGA GGGTCCCTTT GGTGACGTGG 
     3541 GCTGGGCCGG GCCCAGGAAG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     3601 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     3661 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     3721 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     3781 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     3841 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     3901 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     3961 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4021 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4081 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4141 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4201 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4261 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4321 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     4381 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     4441 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     4501 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     4561 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     4621 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     4681 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     4741 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     4801 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     4861 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     4921 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     4981 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5041 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5101 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5161 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5221 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5281 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     5341 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     5401 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     5461 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     5521 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     5581 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     5641 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     5701 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     5761 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     5821 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     5881 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     5941 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6001 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6061 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6121 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6181 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6241 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6301 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     6361 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     6421 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     6481 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     6541 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     6601 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     6661 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6721 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6781 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     6841 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     6901 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     6961 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7021 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7081 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7141 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7201 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7261 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7321 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     7381 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//