LOCUS       RC222093                8529 bp    DNA     circular  SYN 
DEFINITION  MTUS1 (Myc-DDK-tagged)-Human microtubule associated tumor suppressor 1 (MTUS1), transcript variant 2
ACCESSION   NM_001001925
VERSION     NM_001001925.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4686..4777 
                     /label="Myc-DDK"  
     misc_feature    4807..5392 
                     /label="PolyA signal"  
     misc_feature    4864..4883 
                     /label="Restriction Enzyme"  
     misc_feature    5668..6340 
                     /label="ColE1"  
     misc_feature    6770..7564 
                     /label="Kan/Neo"  
     misc_feature    7599..7901 
                     /label="SV40 ori"  
     misc_feature    7987..8464 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4668..4674 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4668 
                     /label="MTUS1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACTGATGAT AATTCAGATG ATAAAATAGA AGATGAATTG CAAACCTTCT 
     1081 TTACCAGTGA TAAAGATGGA AATACACATG CATACAACCC GAAATCACCA CCTACACAAA 
     1141 ACTCTTCAGC CAGCAGTGTG AACTGGAATT CTGCCAACCC AGATGACATG GTGGTTGATT 
     1201 ATGAAACTGA CCCTGCTGTA GTTACTGGTG AAAATATTTC TTTAAGCCTT CAGGGTGTTG 
     1261 AAGTATTTGG TCATGAAAAG TCTTCTAGTG ATTTCATTAG TAAGCAGGTG TTAGATATGC 
     1321 ATAAAGATTC TATTTGTCAG TGTCCTGCAC TTGTAGGTAC TGAGAAGCCC AAATATCTGC 
     1381 AACACAGTTG TCATTCCCTA GAAGCAGTTG AGGGCCAGAG TGTTGAGCCA TCTTTGCCTT 
     1441 TTGTGTGGAA GCCTAATGAC AATTTGAACT GTGCAGGCTA CTGTGATGCC TTGGAGCTAA 
     1501 ACCAAACATT TGACATGACA GTGGATAAAG TTAACTGCAC CTTTATATCA CATCATGCCA 
     1561 TCGGAAAGAG TCAGTCCTTC CATACTGCTG GAAGCCTGCC ACCAACTGGT AGGAGAAGTG 
     1621 GAAGTACATC TTCTTTATCC TATTCCACTT GGACATCTTC CCATTCTGAT AAGACGCATG 
     1681 CAAGAGAAAC TACTTATGAT AGAGAAAGCT TTGAAAACCC TCAAGTCACA CCATCAGAAG 
     1741 CCCAAGACAT GACTTACACA GCATTTTCTG ATGTGGTGAT GCAAAGTGAG GTTTTTGTTT 
     1801 CAGATATTGG AAATCAGTGT GCATGTTCTT CAGGAAAGGT CACCAGTGAG TACACAGATG 
     1861 GATCACAACA AAGACTAGTT GGAGAAAAGG AGACACAAGC ACTAACACCA GTTTCTGATG 
     1921 GCATGGAAGT CCCCAATGAT TCTGCATTAC AAGAGTTCTT TTGTTTATCC CATGATGAAT 
     1981 CCAATAGCGA ACCACATTCA CAGAGCTCAT ACAGGCACAA GGAAATGGGC CAAAATCTGA 
     2041 GAGAGACAGT GTCCTATTGT CTTATTGATG ATGAATGCCC TTTAATGGTG CCAGCTTTTG 
     2101 ATAAGAGCGA AGCTCAAGTG CTGAACCCAG AGCATAAAGT CACTGAGACT GAAGACACAC 
     2161 AAATGGTCTC CAAAGGAAAG GATTTGGGAA CCCAAAATCA TACCTCAGAA TTGATTCTAA 
     2221 GTAGCCCGCC AGGACAAAAG GTGGGCTCGT CATTTGGACT GACTTGGGAT GCAAATGATA 
     2281 TGGTCATTAG CACAGACAAA ACGATGTGCA TGTCAACACC AGTCCTAGAA CCCACAAAAG 
     2341 TAACCTTTTC TGTTTCACCG ATTGAAGCGA CGGAGAAATG TAAGAAAGTG GAGAAGGGTA 
     2401 ATCGAGGGCT TAAAAACATA CCAGACTCGA AGGAGGCACC TGTGAACCTG TGTAAACCCA 
     2461 GTTTAGGAAA ATCAACAATC AAAACGAATA CCCCAATAGG CTGCAAAGTT AGAAAAACTG 
     2521 AAATTATAAG TTACCCAAGA CCAAACTTCA AGAATGTCAA AGCAAAAGTT ATGTCTAGAG 
     2581 CAGTGTTGCA GCCCAAAGAT GCTGCTTTAT CAAAGGTCAC GCCCAGACCT CAGCAGACCA 
     2641 GTGCCTCATC ACCCTCATCA GTGAATTCAA GACAACAAAC AGTCTTGAGC AGAACACCGA 
     2701 GATCTGACTT GAATGCAGAC AAAAAAGCAG AAATTCTAAT TAACAAGACA CATAAGCAGC 
     2761 AGTTTAATAA ACTCATTACT AGCCAGGCTG TGCATGTTAC AACTCATTCT AAAAATGCTT 
     2821 CACACAGGGT TCCAAGAACA ACATCTGCCG TGAAATCGAA TCAGGAAGAT GTTGACAAAG 
     2881 CCAGTTCTTC TAACTCAGCA TGCGAGACCG GGTCCGTTTC TGCGTTGTTT CAGAAGATCA 
     2941 AAGGCATACT CCCTGTTAAA ATGGAAAGTG CAGAATGTTT GGAAATGACC TATGTTCCCA 
     3001 ACATTGATAG GATTAGCCCT GAAAAGAAGG GTGAAAAAGA AAATGGGACA TCTATGGAAA 
     3061 AACAAGAGCT GAAACAAGAG ATTATGAATG AGACTTTTGA ATATGGTTCT CTGTTTTTGG 
     3121 GCTCTGCTTC AAAAACAACG ACCACCTCAG GTAGGAATAT ATCCAAGCCT GACTCCTGCG 
     3181 GTTTGAGGCA AATAGCTGCT CCAAAAGCCA AAGTGGGGCC CCCTGTTTCC TGTTTGAGGC 
     3241 GGAACAGTGA CAATAGAAAT CCCAGTGCTG ATCGAGCCGT ATCTCCTCAG AGGATCAGGC 
     3301 GTGTGTCCAG TTCTGCTGGT AATGCCGCTG TCATCAAATA TGAGGAGAAA CCTCCAAAAC 
     3361 CAGCATTTCA GAATGGTTCC TCAGGATCCT TTTATTTGAA GCCTTTGGTA TCCAGGGCTC 
     3421 ATGTTCACTT GATGAAAACT CCTCCAAAAG GTCCTTCGAG AAAAAATTTA TTTACAGCTC 
     3481 TTAATGCAGT TGAAAAGAGC AGGCAAAAGA ATCCTCGAAG CTTATGTATC CAGCCACAGA 
     3541 CAGCTCCCGA TGCGCTGCCC CCTGAGAAAA CACTTGAATT GACGCAATAT AAAACAAAAT 
     3601 GTGAAAACCA AAGTGGATTT ATCCTGCAGC TCAAGCAGCT TCTTGCCTGT GGTAATACCA 
     3661 AGTTTGAGGC ATTGACAGTT GTGATTCAGC ACCTGCTGTC TGAGCGGGAG GAAGCACTGA 
     3721 AACAACACAA AACCCTATCT CAAGAACTTG TTAACCTCCG GGGAGAGCTA GTCACTGCTT 
     3781 CAACCACCTG TGAGAAATTA GAAAAAGCCA GGAATGAGTT ACAAACAGTG TATGAAGCAT 
     3841 TCGTCCAGCA GCACCAGGCT GAAAAAACAG AACGAGAGAA TCGGCTTAAA GAGTTTTACA 
     3901 CCAGGGAGTA TGAAAAGCTT CGGGACACTT ACATTGAAGA AGCAGAGAAG TACAAAATGC 
     3961 AATTGCAAGA GCAGTTTGAC AACTTAAATG CTGCGCATGA AACCTCTAAG TTGGAAATTG 
     4021 AAGCTAGCCA CTCAGAGAAA CTTGAATTGC TAAAGAAGGC CTATGAAGCC TCCCTTTCAG 
     4081 AAATTAAGAA AGGCCATGAA ATAGAAAAGA AATCGCTTGA AGATTTACTT TCTGAGAAGC 
     4141 AGGAATCGCT AGAGAAGCAA ATCAATGATC TGAAGAGTGA AAATGATGCT TTAAATGAAA 
     4201 AATTGAAATC AGAAGAACAA AAAAGAAGAG CAAGAGAAAA AGCAAATTTG AAAAATCCTC 
     4261 AGATCATGTA TCTAGAACAG GAGTTAGAAA GCCTGAAAGC TGTGTTAGAG ATCAAGAATG 
     4321 AGAAACTGCA TCAACAGGAC ATCAAGTTAA TGAAAATGGA GAAACTGGTG GACAACAACA 
     4381 CAGCATTGGT TGACAAATTG AAGCGTTTCC AGCAGGAGAA TGAAGAATTG AAAGCTCGGA 
     4441 TGGACAAGCA CATGGCAATC TCAAGGCAGC TTTCCACGGA GCAGGCTGTT CTGCAAGAGT 
     4501 CGCTGGAGAA GGAGTCGAAA GTCAACAAGC GACTCTCTAT GGAAAACGAG GAGCTTCTGT 
     4561 GGAAACTGCA CAATGGGGAC CTGTGTAGCC CCAAGAGATC CCCCACATCC TCCGCCATCC 
     4621 CTTTGCAGTC ACCAAGGAAT TCGGGCTCCT TCCCTAGCCC CAGCATTTCA CCCAGAACGC 
     4681 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4741 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4801 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4861 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4921 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4981 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5041 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5101 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5161 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5221 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5281 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5341 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5401 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5461 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5521 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5581 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5641 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5701 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5761 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5821 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5881 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5941 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6001 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6061 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6121 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6181 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6241 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6301 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6361 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6421 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6481 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6541 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6601 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6661 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6721 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6781 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6841 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6901 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6961 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7021 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7081 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7141 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7201 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7261 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7321 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7381 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7441 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7501 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7561 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7621 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7681 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7741 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7801 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7861 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7921 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7981 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8041 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8101 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8161 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8221 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8281 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8341 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8401 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8461 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8521 GCGAATTTT
//