LOCUS       RC222077                8340 bp    DNA     circular  SYN 
DEFINITION  MAML2 (Myc-DDK-tagged)-Human mastermind-like 2 (Drosophila) (MAML2)
ACCESSION   NM_032427
VERSION     NM_032427.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4497..4588 
                     /label="Myc-DDK"  
     misc_feature    4618..5203 
                     /label="PolyA signal"  
     misc_feature    4675..4694 
                     /label="Restriction Enzyme"  
     misc_feature    5479..6151 
                     /label="ColE1"  
     misc_feature    6581..7375 
                     /label="Kan/Neo"  
     misc_feature    7410..7712 
                     /label="SV40 ori"  
     misc_feature    7798..8275 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4479..4485 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4479 
                     /label="MAML2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGGACACA GCGCCCCCGC AGGCCCCCGC AGGAGGGCTA GGGGGGGCCT 
     1081 CTGGGGCGGG GCTCCTTGGA GGGGGCTCAG TCACCCCGAG AGTGCACAGT GCTATCGTGG 
     1141 AGCGCCTCCG GGCTCGGATC GCTGTCTGCC GCCAACACCA CCTGAGCTGT GAAGGACGAT 
     1201 ATGAACGAGG TAGGGCCGAG AGCTCAGACC GGGAAAGAGA AAGCACCTTG CAGCTCCTGA 
     1261 GCCTTGTACA GCATGGCCAG GGGGCAAGGA AAGCTGGCAA ACACACCAAG GCCACCGCCA 
     1321 CTGCTGCCAC CACTACAGCC CCTCCACCGC CCCCTGCTGC CCCTCCTGCG GCCTCCCAAG 
     1381 CAGCAGCAAC AGCAGCCCCA CCGCCCCCAC CAGACTATCA CCATCACCAC CAGCAGCACC 
     1441 TGCTGAACAG TAGCAATAAT GGTGGCAGTG GTGGGATAAA CGGAGAGCAG CAGCCGCCCG 
     1501 CTTCAACCCC AGGGGACCAG AGGAACTCAG CCCTGATTGC GCTCCAGGGT TCCTTGAAAA 
     1561 GAAAACAGGT AGTTAACCTA TCTCCTGCCA ACAGCAAGCG ACCCAATGGC TTTGTGGACA 
     1621 ACTCATTTCT TGATATCAAA AGAATTCGTG TTGGGGAGAA TCTCTCTGCA GGACAAGGTG 
     1681 GCCTCCAAAT AAACAATGGA CAAAGTCAGA TTATGTCAGG GACCTTGCCT ATGAGCCAAG 
     1741 CACCCCTGCG AAAGACTAAC ACTCTGCCAT CCCATACACA TTCTCCTGGC AATGGCCTGT 
     1801 TTAACATGGG CTTAAAGGAG GTAAAGAAGG AGCCAGGAGA GACTCTGTCT TGCAGTAAGC 
     1861 ACATGGATGG CCAAATGACC CAAGAGAATA TTTTTCCTAA TAGGTACGGA GACGACCCTG 
     1921 GAGAACAACT GATGGATCCT GAGCTGCAGG AACTGTTCAA TGAACTGACC AACATATCTG 
     1981 TGCCTCCCAT GAGTGACCTT GAACTGGAGA ACATGATCAA TGCCACCATA AAGCAGGATG 
     2041 ACCCATTTAA CATTGACTTG GGTCAGCAAA GCCAGAGGAG CACACCTAGG CCCTCCTTAC 
     2101 CCATGGAGAA AATAGTGATC AAAAGTGAAT ACTCACCGGG CTTGACTCAG GGCCCCTCAG 
     2161 GCTCTCCTCA GCTGAGGCCC CCATCAGCTG GCCCCGCATT CTCCATGGCC AACTCTGCCC 
     2221 TCTCCACTTC GTCTCCAATC CCTTCAGTCC CTCAGAGCCA GGCTCAGCCT CAGACAGGCT 
     2281 CCGGAGCAAG CCGGGCCTTG CCAAGCTGGC AGGAAGTATC CCATGCCCAG CAGCTCAAAC 
     2341 AGATAGCTGC TAATCGTCAG CAGCATGCCC GGATGCAGCA GCACCAGCAG CAGCACCAGC 
     2401 CTACCAACTG GTCAGCCTTG CCCTCCTCTG CTGGACCATC ACCAGGTCCA TTTGGGCAGG 
     2461 AGAAAATCCC CAGCCCTTCT TTTGGTCAGC AGACATTCAG CCCACAGAGC TCCCCCATGC 
     2521 CTGGGGTAGC TGGCGGCAGC GGCCAGTCGA AAGTAATGGC TAACTACATG TACAAGGCCG 
     2581 GCCCCTCAGC CCAGGGTGGG CACCTAGATG TCCTCATGCA GCAAAAGCCT CAGGATCTCA 
     2641 GTCGAAGTTT TATTAACAAC CCGCACCCAG CCATGGAGCC CCGTCAGGGC AACACCAAGC 
     2701 CTTTGTTTCA TTTTAACTCA GATCAAGCGA ACCAGCAGAT GCCTTCTGTT TTGCCTTCCC 
     2761 AGAACAAGCC TTCTCTCCTA CACTACACCC AACAGCAACA GCAGCAACAG CAGCAGCAGC 
     2821 AGCAGCAGCA GCAGCAGCAA CAGCAGCAGC AGCAGCAACA GCAACAGCAA CAGCAACAGC 
     2881 AGAGTTCAAT TTCAGCTCAA CAACAGCAAC AGCAGCAGAG CTCAATTTCA GCCCAACAGC 
     2941 AGCAGCAGCA GCAACAACAG CAGCAGCAGC AGCAACAACA ACAGCAACAA CAGCAGCAGC 
     3001 AGCAGCAGCA ACAACCATCT TCTCAGCCTG CCCAATCTCT ACCAAGCCAG CCTTTGCTAA 
     3061 GGTCACCTTT GCCACTTCAG CAAAAGCTCC TACTTCAGCA AATGCAGAAT CAGCCCATTG 
     3121 CAGGAATGGG ATACCAAGTC TCCCAACAAC AGAGACAGGA TCAACACTCT GTGGTAGGCC 
     3181 AGAACACAGG CCCCAGTCCA AGTCCTAACC CCTGCTCAAA TCCAAACACT GGAAGTGGTT 
     3241 ACATGAACTC CCAGCAATCA CTGTTGAATC AGCAATTGAT GGGAAAGAAG CAGACTCTAC 
     3301 AGAGGCAGAT CATGGAGCAG AAACAGCAAC TTCTTCTCCA GCAGCAGATG CTGGCTGACG 
     3361 CGGAGAAAAT TGCTCCACAA GATCAGATAA ACCGACATTT GTCAAGGCCA CCTCCAGATT 
     3421 ATAAAGACCA AAGAAGAAAT GTGGGCAATA TGCAACCAAC TGCTCAGTAT TCTGGTGGCT 
     3481 CATCCACAAT AAGCTTAAAC TCTAACCAGG CTTTGGCAAA CCCAGTTTCA ACACACACCA 
     3541 TTTTAACTCC CAATTCCAGC CTCCTGTCTA CTTCTCACGG GACAAGAATG CCATCATTAT 
     3601 CTACAGCAGT TCAGAATATG GGGATGTATG GAAATCTGCC TTGTAATCAA CCTAACACAT 
     3661 ACAGTGTCAC TTCAGGAATG AATCAATTGA CCCAACAGAG AAACCCAAAG CAATTGTTAG 
     3721 CAAATCAAAA CAACCCTATG ATGCCACGGC CACCTACCTT AGGGCCAAGT AATAATAACA 
     3781 ATGTAGCCAC TTTTGGAGCT GGATCTGTTG GTAATTCACA ACAATTGAGA CCAAATTTAA 
     3841 CCCATAGTAT GGCAAGCATG CCACCACAGA GAACATCAAA CGTAATGATC ACATCCAACA 
     3901 CAACTGCACC AAACTGGGCC TCTCAAGAAG GAACAAGCAA ACAGCAAGAA GCCCTGACGT 
     3961 CTGCAGGAGT CCGCTTCCCC ACAGGTACAC CTGCAGCCTA TACCCCAAAT CAGTCACTGC 
     4021 AACAGGCAGT AGGTAGCCAG CAATTTTCCC AGAGGGCAGT GGCTCCTCCT AACCAGTTAA 
     4081 CACCAGCAGT GCAAATGAGA CCCATGAACC AAATGAGCCA AACACTAAAT GGGCAAACCA 
     4141 TGGGTCCCCT CAGGGGTCTG AATCTCAGAC CCAATCAGCT AAGCACACAG ATTTTGCCTA 
     4201 ATTTGAATCA GTCAGGAACA GGGTTGAATC AGTCGAGGAC GGGCATCAAC CAGCCACCAT 
     4261 CCCTGACGCC CAGCAATTTT CCTTCACCCA ACCAAAGTTC CAGGGCTTTT CAAGGAACTG 
     4321 ACCACAGCAG TGACTTAGCT TTTGACTTCC TCAGCCAACA AAATGATAAC ATGGGCCCTG 
     4381 CCCTAAACAG TGATGCTGAT TTCATTGATT CTTTATTGAA GACAGAGCCT GGTAATGATG 
     4441 ACTGGATGAA AGACATCAAT CTTGATGAAA TCTTGGGGAA CAATTCCACG CGTACGCGGC 
     4501 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4561 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4621 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4681 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4741 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4801 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4861 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4921 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4981 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5041 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5101 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5161 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5221 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5281 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5341 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5401 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5461 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5521 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5581 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5641 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5701 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5761 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5821 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5881 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5941 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6001 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6061 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6121 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6181 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6241 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6301 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6361 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6421 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6481 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6541 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6601 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6661 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6721 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6781 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6841 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6901 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6961 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7021 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7081 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7141 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7201 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7261 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7321 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7381 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7441 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7501 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7561 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7621 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7681 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7741 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7801 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7861 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7921 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7981 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8041 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8101 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8161 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8221 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8281 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//