LOCUS       RC222076                8460 bp    DNA     circular  SYN 
DEFINITION  LAMC2 (Myc-DDK-tagged)-Human laminin, gamma 2 (LAMC2), transcript variant 1
ACCESSION   NM_005562
VERSION     NM_005562.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4617..4708 
                     /label="Myc-DDK"  
     misc_feature    4738..5323 
                     /label="PolyA signal"  
     misc_feature    4795..4814 
                     /label="Restriction Enzyme"  
     misc_feature    5599..6271 
                     /label="ColE1"  
     misc_feature    6701..7495 
                     /label="Kan/Neo"  
     misc_feature    7530..7832 
                     /label="SV40 ori"  
     misc_feature    7918..8395 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4599..4605 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4599 
                     /label="LAMC2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTGCGCTC TGGCTGGGCT GCTGCCTCTG CTTCTCGCTC CTCCTGCCCG 
     1081 CAGCCCGGGC CACCTCCAGG AGGGAAGTCT GTGATTGCAA TGGGAAGTCC AGGCAGTGTA 
     1141 TCTTTGATCG GGAACTTCAC AGACAAACTG GTAATGGATT CCGCTGCCTC AACTGCAATG 
     1201 ACAACACTGA TGGCATTCAC TGCGAGAAGT GCAAGAATGG CTTTTACCGG CACAGAGAAA 
     1261 GGGACCGCTG TTTGCCCTGC AATTGTAACT CCAAAGGTTC TCTTAGTGCT CGATGTGACA 
     1321 ACTCTGGACG GTGCAGCTGT AAACCAGGTG TGACAGGAGC CAGATGCGAC CGATGTCTGC 
     1381 CAGGCTTCCA CATGCTCACG GATGCGGGGT GCACCCAAGA CCAGAGACTG CTAGACTCCA 
     1441 AGTGTGACTG TGACCCAGCT GGCATCGCAG GGCCCTGTGA CGCGGGCCGC TGTGTCTGCA 
     1501 AGCCAGCTGT TACTGGAGAA CGCTGTGATA GGTGTCGATC AGGTTACTAT AATCTGGATG 
     1561 GGGGGAACCC TGAGGGCTGT ACCCAGTGTT TCTGCTATGG GCATTCAGCC AGCTGCCGCA 
     1621 GCTCTGCAGA ATACAGTGTC CATAAGATCA CCTCTACCTT TCATCAAGAT GTTGATGGCT 
     1681 GGAAGGCTGT CCAACGAAAT GGGTCTCCTG CAAAGCTCCA ATGGTCACAG CGCCATCAAG 
     1741 ATGTGTTTAG CTCAGCCCAA CGACTAGACC CTGTCTATTT TGTGGCTCCT GCCAAATTTC 
     1801 TTGGGAATCA ACAGGTGAGC TATGGGCAAA GCCTGTCCTT TGACTACCGT GTGGACAGAG 
     1861 GAGGCAGACA CCCATCTGCC CATGATGTGA TTCTGGAAGG TGCTGGTCTA CGGATCACAG 
     1921 CTCCCTTGAT GCCACTTGGC AAGACACTGC CTTGTGGGCT CACCAAGACT TACACATTCA 
     1981 GGTTAAATGA GCATCCAAGC AATAATTGGA GCCCCCAGCT GAGTTACTTT GAGTATCGAA 
     2041 GGTTACTGCG GAATCTCACA GCCCTCCGCA TCCGAGCTAC ATATGGAGAA TACAGTACTG 
     2101 GGTACATTGA CAATGTGACC CTGATTTCAG CCCGCCCTGT CTCTGGAGCC CCAGCACCCT 
     2161 GGGTTGAACA GTGTATATGT CCTGTTGGGT ACAAGGGGCA ATTCTGCCAG GATTGTGCTT 
     2221 CTGGCTACAA GAGAGATTCA GCGAGACTGG GGCCTTTTGG CACCTGTATT CCTTGTAACT 
     2281 GTCAAGGGGG AGGGGCCTGT GATCCAGACA CAGGAGATTG TTATTCAGGG GATGAGAATC 
     2341 CTGACATTGA GTGTGCTGAC TGCCCAATTG GTTTCTACAA CGATCCGCAC GACCCCCGCA 
     2401 GCTGCAAGCC ATGTCCCTGT CATAACGGGT TCAGCTGCTC AGTGATGCCG GAGACGGAGG 
     2461 AGGTGGTGTG CAATAACTGC CCTCCCGGGG TCACCGGTGC CCGCTGTGAG CTCTGTGCTG 
     2521 ATGGCTACTT TGGGGACCCC TTTGGTGAAC ATGGCCCAGT GAGGCCTTGT CAGCCCTGTC 
     2581 AATGCAACAA CAATGTGGAC CCCAGTGCCT CTGGGAATTG TGACCGGCTG ACAGGCAGGT 
     2641 GTTTGAAGTG TATCCACAAC ACAGCCGGCA TCTACTGCGA CCAGTGCAAA GCAGGCTACT 
     2701 TCGGGGACCC ATTGGCTCCC AACCCAGCAG ACAAGTGTCG AGCTTGCAAC TGTAACCCCA 
     2761 TGGGCTCAGA GCCTGTAGGA TGTCGAAGTG ATGGCACCTG TGTTTGCAAG CCAGGATTTG 
     2821 GTGGCCCCAA CTGTGAGCAT GGAGCATTCA GCTGTCCAGC TTGCTATAAT CAAGTGAAGA 
     2881 TTCAGATGGA TCAGTTTATG CAGCAGCTTC AGAGAATGGA GGCCCTGATT TCAAAGGCTC 
     2941 AGGGTGGTGA TGGAGTAGTA CCTGATACAG AGCTGGAAGG CAGGATGCAG CAGGCTGAGC 
     3001 AGGCCCTTCA GGACATTCTG AGAGATGCCC AGATTTCAGA AGGTGCTAGC AGATCCCTTG 
     3061 GTCTCCAGTT GGCCAAGGTG AGGAGCCAAG AGAACAGCTA CCAGAGCCGC CTGGATGACC 
     3121 TCAAGATGAC TGTGGAAAGA GTTCGGGCTC TGGGAAGTCA GTACCAGAAC CGAGTTCGGG 
     3181 ATACTCACAG GCTCATCACT CAGATGCAGC TGAGCCTGGC AGAAAGTGAA GCTTCCTTGG 
     3241 GAAACACTAA CATTCCTGCC TCAGACCACT ACGTGGGGCC AAATGGCTTT AAAAGTCTGG 
     3301 CTCAGGAGGC CACAAGATTA GCAGAAAGCC ACGTTGAGTC AGCCAGTAAC ATGGAGCAAC 
     3361 TGACAAGGGA AACTGAGGAC TATTCCAAAC AAGCCCTCTC ACTGGTGCGC AAGGCCCTGC 
     3421 ATGAAGGAGT CGGAAGCGGA AGCGGTAGCC CGGACGGTGC TGTGGTGCAA GGGCTTGTGG 
     3481 AAAAATTGGA GAAAACCAAG TCCCTGGCCC AGCAGTTGAC AAGGGAGGCC ACTCAAGCGG 
     3541 AAATTGAAGC AGATAGGTCT TATCAGCACA GTCTCCGCCT CCTGGATTCA GTGTCTCGGC 
     3601 TTCAGGGAGT CAGTGATCAG TCCTTTCAGG TGGAAGAAGC AAAGAGGATC AAACAAAAAG 
     3661 CGGATTCACT CTCAACGCTG GTAACCAGGC ATATGGATGA GTTCAAGCGT ACACAAAAGA 
     3721 ATCTGGGAAA CTGGAAAGAA GAAGCACAGC AGCTCTTACA GAATGGAAAA AGTGGGAGAG 
     3781 AGAAATCAGA TCAGCTGCTT TCCCGTGCCA ATCTTGCTAA AAGCAGAGCA CAAGAAGCAC 
     3841 TGAGTATGGG CAATGCCACT TTTTATGAAG TTGAGAGCAT CCTTAAAAAC CTCAGAGAGT 
     3901 TTGACCTGCA GGTGGACAAC AGAAAAGCAG AAGCTGAAGA AGCCATGAAG AGACTCTCCT 
     3961 ACATCAGCCA GAAGGTTTCA GATGCCAGTG ACAAGACCCA GCAAGCAGAA AGAGCCCTGG 
     4021 GGAGCGCTGC TGCTGATGCA CAGAGGGCAA AGAATGGGGC CGGGGAGGCC CTGGAAATCT 
     4081 CCAGTGAGAT TGAACAGGAG ATTGGGAGTC TGAACTTGGA AGCCAATGTG ACAGCAGATG 
     4141 GAGCCTTGGC CATGGAAAAG GGACTGGCCT CTCTGAAGAG TGAGATGAGG GAAGTGGAAG 
     4201 GAGAGCTGGA AAGGAAGGAG CTGGAGTTTG ACACGAATAT GGATGCAGTA CAGATGGTGA 
     4261 TTACAGAAGC CCAGAAGGTT GATACCAGAG CCAAGAACGC TGGGGTTACA ATCCAAGACA 
     4321 CACTCAACAC ATTAGACGGC CTCCTGCATC TGATGGACCA GCCTCTCAGT GTAGATGAAG 
     4381 AGGGGCTGGT CTTACTGGAG CAGAAGCTTT CCCGAGCCAA GACCCAGATC AACAGCCAAC 
     4441 TGCGGCCCAT GATGTCAGAG CTGGAAGAGA GGGCACGTCA GCAGAGGGGC CACCTCCATT 
     4501 TGCTGGAGAC AAGCATAGAT GGGATTCTGG CTGATGTGAA GAACTTGGAG AACATTAGGG 
     4561 ACAACCTGCC CCCAGGCTGC TACAATACCC AGGCTCTTGA GCAACAGACG CGTACGCGGC 
     4621 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4681 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4741 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4801 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4861 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4921 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4981 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     5041 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5101 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5161 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5221 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5281 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5341 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5401 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5461 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5521 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5581 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5641 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5701 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5761 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5821 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5881 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5941 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     6001 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6061 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6121 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6181 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6241 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6301 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6361 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6421 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6481 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6541 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6601 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6661 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6721 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6781 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6841 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6901 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6961 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     7021 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7081 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7141 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7201 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7261 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7321 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7381 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7441 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7501 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7561 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7621 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7681 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7741 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7801 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7861 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7921 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7981 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     8041 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     8101 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8161 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8221 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8281 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8341 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8401 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//