LOCUS       RC222063                9339 bp    DNA     circular  SYN 
DEFINITION  CUX2 (Myc-DDK-tagged)-Human cut-like homeobox 2 (CUX2)
ACCESSION   NM_015267
VERSION     NM_015267.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5496..5587 
                     /label="Myc-DDK"  
     misc_feature    5617..6202 
                     /label="PolyA signal"  
     misc_feature    5674..5693 
                     /label="Restriction Enzyme"  
     misc_feature    6478..7150 
                     /label="ColE1"  
     misc_feature    7580..8374 
                     /label="Kan/Neo"  
     misc_feature    8409..8711 
                     /label="SV40 ori"  
     misc_feature    8797..9274 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5478..5484 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5478 
                     /label="CUX2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCGCCAAT GTGGGATCGA TGTTTCAATA TTGGAAGCGA TTTGATCTAC 
     1081 GGCGACTCCA GAAGGAGCTT AATTCCGTCG CTTCTGAGCT GTCTGCACGG CAGGAGGAGA 
     1141 GTGAACATTC TCATAAACAT TTAATTGAAC TCCGCCGGGA ATTTAAGAAA AATGTACCTG 
     1201 AGGAAATCAG AGAGATGGTG GCTCCTGTAT TAAAAAGCTT CCAAGCCGAG GTGGTGGCCC 
     1261 TTAGTAAGAG AAGTCAGGAG GCGGAGGCTG CTTTTCTGAG TGTTTACAAG CAATTAATTG 
     1321 AAGCACCAGA CCCCGTGCCT GTGTTTGAGG CGGCACGCAG CCTAGACGAC AGACTGCAGC 
     1381 CCCCCAGCTT TGACCCCAGT GGGCAGCCCC GGCGAGACCT CCACACTTCG TGGAAGAGGA 
     1441 ACCCCGAGCT CCTCAGCCCC AAAGAGCAGA GAGAGGGGAC GTCGCCTGCC GGGCCCACGC 
     1501 TGACCGAGGG AAGCCGCCTC CCAGGCATTC CCGGGAAAGC CCTCCTGACA GAAACCTTGC 
     1561 TGCAGAGAAA TGAGGCGGAA AAACAAAAGG GCCTTCAAGA AGTACAGATC ACTTTGGCGG 
     1621 CCAGACTGGG GGAGGCAGAG GAGAAAATCA AAGTCCTACA TTCAGCGCTA AAGGCTACGC 
     1681 AGGCAGAGCT GCTAGAGCTG CGGCGGAAGT ACGACGAGGA GGCAGCATCC AAGGCAGATG 
     1741 AAGTCGGCCT GATCATGACC AACCTGGAGA AAGCTAATCA GCGAGCTGAG GCTGCCCAGC 
     1801 GGGAGGTGGA AAGTCTCCGG GAACAGCTGG CCTCTGTCAA CAGCTCCATC CGCCTGGCTT 
     1861 GCTGCTCTCC CCAGGGGCCC AGTGGGGATA AGGTGAACTT CACTCTGTGC TCGGGCCCTC 
     1921 GGCTGGAGGC CGCGCTGGCC TCCAAGGACA GGGAGATCCT GCGGCTGCTG AAGGACGTGC 
     1981 AGCACCTCCA GAGCTCACTG CAGGAGCTGG AGGAGGCATC CGCCAACCAG ATCGCCGACC 
     2041 TGGAGCGGCA GCTCACGGCC AAGTCCGAGG CCATAGAAAA GCTGGAAGAG AAGCTCCAGG 
     2101 CCCAGTCTGA CTATGAGGAA ATTAAAACGG AGCTGAGCAT CCTGAAAGCC ATGAAGCTGG 
     2161 CCTCCAGCAC CTGCAGCCTC CCCCAGGGCA TGGCCAAGCC TGAAGACTCA CTGCTTATTG 
     2221 CAAAGGAGGC CTTCTTCCCC ACGCAGAAAT TCCTTCTGGA GAAGCCCAGC CTCCTGGCCA 
     2281 GCCCTGAGGA AGACCCATCA GAGGACGATT CCATCAAGGA TTCACTGGGC ACGGAGCAGT 
     2341 CCTACCCCTC CCCTCAGCAG CTCCCACCTC CACCAGGGCC AGAAGACCCC CTGTCTCCCA 
     2401 GCCCCGGGCA GCCCCTGCTG GGCCCCAGCT TGGGGCCTGA CGGCACTCGG ACTTTCTCGC 
     2461 TGTCCCCCTT CCCCAGCCTG GCATCAGGGG AGAGACTGAT GATGCCCCCA GCCGCCTTCA 
     2521 AGGGAGAGGC GGGCGGCCTG CTGGTGTTCC CCCCAGCCTT CTATGGCGCC AAGCCCCCCA 
     2581 CAGCCCCTGC CACCCCGGCC CCTGGCCCTG AGCCACTGGG CGGTCCTGAG CCCGCGGATG 
     2641 GTGGTGGGGG CGGAGCGGCG GGGCCCGGGG CAGAGGAGGA GCAGCTGGAC ACGGCAGAGA 
     2701 TCGCCTTCCA GGTGAAGGAG CAGCTGCTGA AACACAACAT CGGGCAGCGG GTGTTTGGGC 
     2761 ATTACGTGCT GGGGCTGTCG CAGGGCTCGG TCAGCGAGAT CCTAGCCCGG CCCAAGCCCT 
     2821 GGCGCAAGCT CACGGTGAAG GGCAAGGAGC CCTTCATCAA GATGAAGCAG TTCCTGTCGG 
     2881 ATGAGCAGAA TGTACTGGCG CTCAGGACCA TCCAAGTGCG GCAGCGAGGC AGCATCACCC 
     2941 CGAGAATCCG CACGCCTGAG ACAGGCTCAG ACGACGCCAT CAAGAGCATT CTAGAGCAGG 
     3001 CCAAGAAGGA GATCGAGTCG CAGAAGGGCG GCGAGCCCAA GACCTCGGTG GCCCCGCTGA 
     3061 GCATCGCCAA CGGCACGACC CCCGCCAGCA CCTCGGAGGA CGCCATCAAG AGCATCCTGG 
     3121 AGCAGGCACG CCGTGAGATG CAGGCGCAAC AGCAGGCGCT GCTGGAGATG GAGGTGGCGC 
     3181 CCAGGGGCCG CTCGGTGCCC CCCTCGCCCC CGGAGCGGCC ATCACTGGCC ACCGCGAGCC 
     3241 AGAACGGGGC CCCGGCCTTG GTGAAGCAGG AGGAGGGCAG CGGGGGCCCC GCGCAGGCGC 
     3301 CGCTCCCGGT CCTGTCCCCC GCCGCCTTCG TGCAGAGCAT CATCCGCAAG GTCAAGTCCG 
     3361 AGATCGGCGA CGCCGGCTAC TTCGACCACC ACTGGGCCTC CGACCGCGGC CTGCTCAGCC 
     3421 GCCCCTACGC CTCCGTGTCG CCCTCGCTGT CCTCCTCCTC CTCCTCTGGC TACTCTGGCC 
     3481 AGCCCAACGG CCGCGCCTGG CCCCGCGGGG ACGAGGCCCC TGTGCCCCCC GAGGACGAGG 
     3541 CGGCGGCAGG GGCGGAGGAC GAACCCCCCA GGACGGGCGA GCTCAAGGCT GAGGGCGCGA 
     3601 CGGCCGAGGC GGGCGCGCGG CTGCCCTACT ACCCGGCCTA CGTGCCGCGC ACCCTGAAGC 
     3661 CCACCGTGCC GCCGCTGACC CCCGAGCAGT ACGAGCTGTA CATGTACCGT GAGGTAGACA 
     3721 CGCTGGAGCT CACCCGCCAG GTCAAGGAGA AGCTGGCCAA GAACGGCATC TGCCAGAGGA 
     3781 TCTTCGGGGA GAAGGTGCTG GGCCTGTCAC AGGGCAGCGT GAGCGACATG CTGTCCCGGC 
     3841 CGAAGCCATG GAGCAAGCTG ACGCAGAAGG GGCGGGAGCC CTTCATCCGC ATGCAGCTGT 
     3901 GGCTCTCTGA CCAGCTCGGC CAGGCAGTGG GCCAGCAGCC TGGTGCCTCC CAGGCCAGTC 
     3961 CCACAGAACC AAGGTCCTCA CCATCCCCAC CCCCCAGCCC CACAGAGCCT GAGAAGAGCT 
     4021 CCCAGGAGCC GTTGAGCCTG TCCCTGGAGA GCAGCAAGGA GAACCAGCAG CCAGAGGGCC 
     4081 GCTCCAGCTC CTCGTTGAGC GGGAAGATGT ACTCAGGCAG CCAGGCCCCA GGGGGCATCC 
     4141 AGGAGATCGT GGCCATGTCC CCCGAGCTGG ACACGTACTC CATCACCAAG AGGGTGAAGG 
     4201 AGGTCCTCAC AGACAACAAT CTAGGGCAGC GGCTGTTTGG GGAAAGCATC CTGGGTCTGA 
     4261 CACAGGGCTC CGTGTCTGAC CTGCTGTCCC GGCCCAAACC CTGGCACAAG CTGAGCCTGA 
     4321 AGGGGCGGGA GCCTTTTGTC CGCATGCAGC TGTGGCTCAA TGACCCCCAT AACGTGGAGA 
     4381 AGCTGAGGGA TATGAAGAAG CTGGAGAAGA AAGCCTACCT GAAACGTCGC TATGGCCTCA 
     4441 TCAGCACCGG CTCAGACAGT GAGTCCCCGG CCACCCGCTC AGAGTGCCCC AGCCCCTGCC 
     4501 TGCAGCCCCA GGACCTGAGC CTCCTGCAGA TCAAGAAGCC CCGGGTGGTG CTGGCACCCG 
     4561 AGGAGAAGGA GGCACTGCGG AAGGCCTATC AGCTGGAACC CTACCCCTCG CAGCAGACCA 
     4621 TCGAGCTCCT CTCCTTCCAG CTCAACCTCA AGACCAACAC CGTCATCAAC TGGTTCCACA 
     4681 ACTACAGGTC CCGGATGCGC CGGGAGATGT TGGTGGAGGG GACCCAGGAT GAGCCAGACC 
     4741 TTGATCCAAG CGGGGGTCCT GGAATCCTAC CGCCAGGCCA CTCCCACCCA GACCCCACCC 
     4801 CGCAGAGCCC TGACTCTGAG ACTGAGGACC AGAAGCCAAC CGTGAAGGAA CTGGAGCTTC 
     4861 AGGAGGGCCC TGAGGAGAAC AGCACACCCC TGACCACCCA GGACAAGGCC CAAGTGAGGA 
     4921 TCAAGCAGGA ACAGATGGAG GAGGATGCTG AGGAAGAGGC AGGCAGCCAG CCCCAGGACT 
     4981 CAGGGGAGCT GGACAAAGGC CAAGGTCCCC CCAAAGAGGA GCATCCCGAC CCTCCGGGTA 
     5041 ATGATGGACT CCCAAAAGTG GCTCCCGGGC CCCTCCTTCC AGGTGGATCC ACCCCAGACT 
     5101 GTCCCTCACT TCATCCCCAA CAGGAGAGTG AGGCCGGGGA GCGACTTCAC CCGGACCCTT 
     5161 TAAGTTTTAA GTCAGCCTCA GAGTCCTCAC GCTGCAGCCT GGAGGTGTCA CTGAACTCGC 
     5221 CCTCGGCCGC CTCCTCACCA GGCCTCATGA TGTCTGTGTC ACCTGTCCCC TCCTCCTCAG 
     5281 CTCCCATCTC CCCATCCCCA CCTGGCGCCC CCCCTGCCAA AGTGCCGAGT GCCAGCCCCA 
     5341 CTGCTGACAT GGCTGGAGCC TTGCACCCCA GTGCCAAGGT GAACCCCAAC TTGCAGCGGC 
     5401 GGCATGAGAA GATGGCCAAT CTGAACAACA TCATTTACCG AGTAGAGCGG GCTGCCAATC 
     5461 GGGAGGAGGC CCTGGAGTGG GAGTTCACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     5521 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     5581 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     5641 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     5701 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     5761 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     5821 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     5881 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     5941 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     6001 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     6061 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     6121 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     6181 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     6241 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     6301 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     6361 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     6421 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     6481 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     6541 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     6601 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     6661 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     6721 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     6781 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     6841 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     6901 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6961 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     7021 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     7081 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     7141 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     7201 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     7261 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     7321 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     7381 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     7441 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     7501 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     7561 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     7621 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     7681 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     7741 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     7801 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     7861 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     7921 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7981 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     8041 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     8101 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     8161 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     8221 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     8281 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     8341 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     8401 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     8461 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     8521 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     8581 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8641 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8701 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     8761 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     8821 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     8881 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     8941 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     9001 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     9061 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     9121 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     9181 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     9241 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     9301 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//