LOCUS       RC222055                9378 bp    DNA     circular  SYN 
DEFINITION  RAPGEF2 (Myc-DDK-tagged)-Human Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2)
ACCESSION   NM_014247
VERSION     NM_014247.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5535..5626 
                     /label="Myc-DDK"  
     misc_feature    5656..6241 
                     /label="PolyA signal"  
     misc_feature    5713..5732 
                     /label="Restriction Enzyme"  
     misc_feature    6517..7189 
                     /label="ColE1"  
     misc_feature    7619..8413 
                     /label="Kan/Neo"  
     misc_feature    8448..8750 
                     /label="SV40 ori"  
     misc_feature    8836..9313 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5517..5523 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5517 
                     /label="RAPGEF2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAACCACTA GCAATCCCAG CTAACCATGG AGTTATGGGC CAGCAGGAGA 
     1081 AACACTCACT TCCTGCAGAT TTCACAAAAC TGCATCTTAC TGACAGTCTC CACCCACAGG 
     1141 TGACCCACGT TTCTTCTAGC CATTCAGGAT GTAGTATCAC TAGTGATTCT GGGAGCAGCA 
     1201 GTCTTTCTGA TATCTACCAG GCCACAGAAA GCGAGGCTGG TGATATGGAC CTGAGTGGGT 
     1261 TGCCAGAAAC AGCAGTGGAT TCCGAAGACG ACGACGATGA AGAAGACATT GAGAGAGCAT 
     1321 CAGATCCTCT GATGAGCAGG GACATTGTGA GAGACTGCCT AGAGAAGGAC CCAATTGACC 
     1381 GGACAGATGA TGACATTGAA CAACTCTTGG AATTTATGCA CCAGTTGCCT GCTTTTGCCA 
     1441 ATATGACAAT GTCAGTGAGG CGAGAACTCT GTGCTGTGAT GGTGTTCGCA GTGGTGGAAA 
     1501 GAGCAGGGAC CATAGTGTTA AATGATGGTG AAGAGCTGGA CTCCTGGTCA GTGATTCTCA 
     1561 ATGGATCTGT GGAAGTGACT TATCCAGATG GAAAAGCAGA AATACTGTGC ATGGGAAATA 
     1621 GTTTTGGTGT CTCTCCTACC ATGGACAAAG AATACATGAA AGGAGTGATG AGAACAAAGG 
     1681 TGGATGACTG CCAGTTTGTC TGCATAGCCC AGCAAGATTA CTGCCGTATT CTCAATCAAG 
     1741 TAGAAAAGAA CATGCAAAAA GTTGAAGAGG AAGGAGAGAT TGTTATGGTG AAAGAACACC 
     1801 GAGAACTTGA TCGAACTGGA ACAAGAAAGG GACACATTGT CATCAAGGGT ACCTCAGAAA 
     1861 GGTTAACAAT GCATTTGGTG GAAGAGCATT CAGTAGTAGA TCCAACATTC ATAGAAGACT 
     1921 TTCTGTTGAC CTATAGGACT TTTCTTTCTA GCCCAATGGA AGTGGGCAAA AAGTTATTGG 
     1981 AGTGGTTTAA TGACCCGAGC CTCAGGGATA AGGTTACACG GGTAGTATTA TTGTGGGTAA 
     2041 ATAATCACTT CAATGACTTT GAAGGAGATC CTGCAATGAC TCGATTTTTA GAAGAATTTG 
     2101 AAAACAATCT GGAAAGAGAG AAAATGGGTG GACACCTAAG GCTGTTGAAT ATCGCGTGTG 
     2161 CTGCTAAAGC AAAAAGAAGA TTGATGACGT TAACAAAACC ATCCCGAGAA GCTCCTTTGC 
     2221 CTTTTATCTT ACTTGGAGGC TCTGAGAAGG GATTTGGAAT CTTTGTTGAC AGTGTAGATT 
     2281 CAGGTAGCAA AGCAACTGAA GCAGGCTTGA AACGGGGGGA TCAGATATTA GAAGTAAATG 
     2341 GCCAAAACTT TGAAAACATT CAGCTGTCAA AAGCTATGGA AATTCTTAGA AATAACACAC 
     2401 ATTTATCTAT CACTGTGAAA ACCAATTTAT TTGTATTTAA AGAACTTCTA ACAAGATTGT 
     2461 CAGAAGAGAA AAGAAATGGT GCCCCCCACC TTCCTAAAAT TGGTGACATT AAAAAGGCCA 
     2521 GTCGCTACTC CATTCCAGAT CTTGCTGTAG ATGTAGAACA GGTGATAGGA CTTGAAAAAG 
     2581 TGAACAAAAA AAGTAAAGCC AACACTGTGG GAGGAAGGAA CAAGCTGAAA AAGATACTCG 
     2641 ACAAGACTCG GATCAGTATC TTGCCACAGA AACCATACAA TGATATTGGG ATTGGTCAGT 
     2701 CTCAAGATGA CAGCATAGTA GGATTAAGGC AGACAAAGCA CATCCCAACT GCATTGCCTG 
     2761 TCAGTGGAAC CTTATCATCC AGTAATCCTG ATTTATTGCA GTCACATCAT CGCATTTTAG 
     2821 ACTTCAGTGC TACTCCTGAC TTGCCAGATC AAGTGCTAAG GGTTTTTAAG GCTGATCAGC 
     2881 AAAGCCGCTA CATCATGATC AGTAAGGACA CTACAGCAAA GGAAGTGGTC ATTCAGGCTA 
     2941 TCAGGGAGTT TGCTGTTACT GCCACCCCGG ATCAATATTC ACTATGTGAG GTCTCTGTCA 
     3001 CACCTGAGGG AGTAATCAAA CAAAGAAGAC TTCCAGATCA GCTTTCCAAA CTTGCAGACA 
     3061 GAATACAACT GAGTGGAAGG TATTATCTGA AAAACAACAT GGAAACAGAA ACTCTTTGTT 
     3121 CAGATGAAGA TGCTCAGGAG TTGTTGAGAG AGAGTCAAAT TTCCCTCCTT CAGCTCAGCA 
     3181 CTGTGGAAGT TGCAACACAG CTCTCTATGC GAAATTTTGA ACTCTTTCGC AACATTGAAC 
     3241 CTACTGAATA TATAGATGAT TTATTTAAAC TCAGATCAAA AACCAGCTGT GCCAACCTGA 
     3301 AGAGATTTGA AGAAGTCATT AACCAGGAAA CATTTTGGGT AGCATCTGAA ATTCTCAGAG 
     3361 AAACAAACCA GCTGAAGAGG ATGAAGATCA TTAAGCATTT CATCAAGATA GCACTGCACT 
     3421 GTAGGGAATG CAAGAATTTT AACTCAATGT TTGCAATCAT CAGTGGCCTA AACCTGGCAC 
     3481 CAGTGGCAAG ACTGCGAACG ACCTGGGAGA AACTTCCCAA TAAATACGAA AAACTATTTC 
     3541 AAGATCTCCA AGACCTGTTT GATCCTTCCA GAAACATGGC AAAATATCGT AATGTTCTCA 
     3601 ATAGTCAAAA TCTACAACCT CCCATAATCC CTCTATTCCC AGTTATCAAA AAGGATCTCA 
     3661 CCTTCCTTCA CGAAGGAAAT GACTCAAAAG TAGACGGGCT GGTCAATTTT GAGAAGCTAA 
     3721 GGATGATTGC AAAAGAAATT CGTCACGTTG GCCGAATGGC TTCAGTGAAC ATGGACCCTG 
     3781 CCCTCATGTT CAGGACTCGG AAGAAGAAAT GGCGGAGTTT GGGGTCTCTC AGCCAGGGTA 
     3841 GTACAAATGC AACAGTGCTA GATGTTGCTC AGACAGGTGG TCATAAAAAG CGGGTACGTC 
     3901 GTAGTTCCTT TCTCAATGCC AAAAAGCTTT ATGAAGATGC CCAAATGGCT CGAAAAGTGA 
     3961 AGCAGTACCT TTCCAATTTG GAGCTAGAAA TGGACGAGGA GAGTCTTCAG ACATTATCTC 
     4021 TGCAGTGTGA GCCAGCAACC AACACATTGC CTAAGAATCC TGGTGACAAA AAGCCTGTCA 
     4081 AATCCGAGAC CTCTCCAGTA GCTCCAAGGG CAGGGTCACA ACAGAAAGCT CAGTCCCTGC 
     4141 CACAGCCCCA GCAGCAGCCA CCACCAGCAC ATAAAATCAA CCAGGGACTA CAGGTTCCCG 
     4201 CCGTGTCCCT TTATCCTTCA CGGAAGAAAG TGCCCGTAAA GGATCTCCCA CCTTTTGGCA 
     4261 TAAACTCTCC ACAAGCTTTA AAAAAAATTC TTTCTTTGTC TGAAGAAGGA AGTTTGGAAC 
     4321 GTCACAAGAA ACAGGCTGAA GATACAATAT CAAATGCATC TTCGCAGCTT TCTTCTCCTC 
     4381 CTACTTCTCC ACAGAGTTCT CCAAGGAAAG GCTATACTTT GGCTCCCAGT GGTACTGTGG 
     4441 ATAATTTTTC AGATTCTGGT CACAGTGAAA TTTCTTCACG ATCCAGTATT GTTAGCAATT 
     4501 CGTCTTTTGA CTCAGTGCCA GTCTCACTGC ACGATGAGAG GCGCCAGAGG CATTCTGTCA 
     4561 GCATCGTGGA AACAAACCTA GGGATGGGCA GGATGGAGAG GCGGACCATG ATTGAACCTG 
     4621 ATCAGTATAG CTTGGGGTCC TATGCACCAA TGTCCGAGGG CCGAGGCTTA TATGCTACAG 
     4681 CTACAGTAAT TTCTTCTCCA AGCACAGAGG AACTTTCCCA GGATCAGGGG GATCGCGCGT 
     4741 CACTTGATGC TGCTGACAGT GGCCGTGGGA GCTGGACGTC ATGCTCAAGT GGCTCCCATG 
     4801 ATAATATACA GACGATCCAG CACCAGAGAA GCTGGGAGAC TCTTCCATTC GGGCATACTC 
     4861 ACTTTGATTA TTCAGGGGAT CCTGCAGGTT TATGGGCATC AAGCAGCCAT ATGGACCAAA 
     4921 TTATGTTTTC TGATCATAGC ACAAAGTATA ACAGGCAAAA TCAAAGTAGA GAGAGCCTTG 
     4981 AACAAGCCCA GTCCCGAGCA AGCTGGGCGT CTTCCACAGG TTACTGGGGA GAAGACTCAG 
     5041 AAGGTGACAC AGGCACAATA AAGCGGAGGG GTGGAAAGGA TGTTTCCATT GAAGCCGAAA 
     5101 GCAGTAGCCT AACGTCTGTG ACTACGGAAG AAACCAAGCC TGTCCCCATG CCTGCCCACA 
     5161 TAGCTGTGGC ATCAAGTACT ACAAAGGGGC TCATTGCACG AAAGGAGGGC AGGTATCGAG 
     5221 AGCCCCCGCC CACCCCTCCC GGCTACATTG GAATTCCCAT TACTGACTTT CCAGAAGGGC 
     5281 ACTCCCATCC AGCCAGGAAA CCGCCGGACT ACAACGTGGC CCTTCAGAGA TCGCGGATGG 
     5341 TCGCACGATC CTCCGACACA GCTGGGCCTT CATCCGTACA GCAGCCACAT GGGCATCCCA 
     5401 CCAGCAGCAG GCCTGTGAAC AAACCTCAGT GGCATAAACC GAACGAGTCT GACCCGCGCC 
     5461 TCGCCCCTTA TCAGTCCCAA GGGTTTTCCA CCGAGGAGGA TGAAGATGAA CAAGTTTCTG 
     5521 CTGTTACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     5581 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     5641 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     5701 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     5761 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     5821 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5881 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5941 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     6001 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     6061 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     6121 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     6181 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     6241 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     6301 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     6361 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     6421 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     6481 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     6541 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     6601 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     6661 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     6721 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     6781 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6841 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6901 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6961 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     7021 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     7081 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     7141 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     7201 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     7261 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     7321 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     7381 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     7441 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     7501 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     7561 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     7621 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     7681 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     7741 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     7801 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7861 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7921 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7981 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     8041 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     8101 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     8161 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     8221 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     8281 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     8341 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     8401 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     8461 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     8521 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     8581 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     8641 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8701 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8761 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     8821 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8881 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8941 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     9001 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     9061 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     9121 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     9181 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     9241 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     9301 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     9361 AAATTTAACG CGAATTTT
//