LOCUS       RC222049                8901 bp    DNA     circular  SYN 
DEFINITION  ADAMTS13 (Myc-DDK-tagged)-Human ADAM metallopeptidase with thrombospondin type 1 motif, 13 (ADAMTS13), transcript variant 3
ACCESSION   NM_139026
VERSION     NM_139026.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5058..5149 
                     /label="Myc-DDK"  
     misc_feature    5179..5764 
                     /label="PolyA signal"  
     misc_feature    5236..5255 
                     /label="Restriction Enzyme"  
     misc_feature    6040..6712 
                     /label="ColE1"  
     misc_feature    7142..7936 
                     /label="Kan/Neo"  
     misc_feature    7971..8273 
                     /label="SV40 ori"  
     misc_feature    8359..8836 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5040..5046 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5040 
                     /label="ADAMTS13"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCACCAGCGT CACCCCCGGG CAAGATGCCC TCCCCTCTGT GTGGCCGGAA 
     1081 TCCTTGCCTG TGGCTTTCTC CTGGGCTGCT GGGGACCCTC CCATTTCCAG CAGAGTTGTC 
     1141 TTCAGGCTTT GGAGCCACAG GCCGTGTCTT CTTACTTGAG CCCTGGTGCT CCCTTAAAAG 
     1201 GCCGCCCTCC TTCCCCTGGC TTCCAGAGGC AGAGGCAGAG GCAGAGGCGG GCTGCAGGCG 
     1261 GCATCCTACA CCTGGAGCTG CTGGTGGCCG TGGGCCCCGA TGTCTTCCAG GCTCACCAGG 
     1321 AGGACACAGA GCGCTATGTG CTCACCAACC TCAACATCGG GGCAGAACTG CTTCGGGACC 
     1381 CGTCCCTGGG GGCTCAGTTT CGGGTGCACC TGGTGAAGAT GGTCATTCTG ACAGAGCCTG 
     1441 AGGGTGCCCC AAATATCACA GCCAACCTCA CCTCGTCCCT GCTGAGCGTC TGTGGGTGGA 
     1501 GCCAGACCAT CAACCCTGAG GACGACACGG ATCCTGGCCA TGCTGACCTG GTCCTCTATA 
     1561 TCACTAGGTT TGACCTGGAG TTGCCTGATG GTAACCGGCA GGTGCGGGGC GTCACCCAGC 
     1621 TGGGCGGTGC CTGCTCCCCA ACCTGGAGCT GCCTCATTAC CGAGGACACT GGCTTCGACC 
     1681 TGGGAGTCAC CATTGCCCAT GAGATTGGGC ACAGCTTCGG CCTGGAGCAC GACGGCGCGC 
     1741 CCGGCAGCGG CTGCGGCCCC AGCGGACACG TGATGGCTTC GGACGGCGCC GCGCCCCGCG 
     1801 CCGGCCTCGC CTGGTCCCCC TGCAGCCGCC GGCAGCTGCT GAGCCTGCTC AGCGCCAACG 
     1861 AGCAGTGCCG CGTGGCCTTC GGCCCCAAGG CTGTCGCCTG CACCTTCGCC AGGGAGCACC 
     1921 TGGATATGTG CCAGGCCCTC TCCTGCCACA CAGACCCGCT GGACCAAAGC AGCTGCAGCC 
     1981 GCCTCCTCGT TCCTCTCCTG GATGGGACAG AATGTGGCGT GGAGAAGTGG TGCTCCAAGG 
     2041 GTCGCTGCCG CTCCCTGGTG GAGCTGACCC CCATAGCAGC AGTGCATGGG CGCTGGTCTA 
     2101 GCTGGGGTCC CCGAAGTCCT TGCTCCCGCT CCTGCGGAGG AGGTGTGGTC ACCAGGAGGC 
     2161 GGCAGTGCAA CAACCCCAGA CCTGCCTTTG GGGGGCGTGC ATGTGTTGGT GCTGACCTCC 
     2221 AGGCCGAGAT GTGCAACACT CAGGCCTGCG AGAAGACCCA GCTGGAGTTC ATGTCGCAAC 
     2281 AGTGCGCCAG GACCGACGGC CAGCCGCTGC GCTCCTCCCC TGGCGGCGCC TCCTTCTACC 
     2341 ACTGGGGTGC TGCTGTACCA CACAGCCAAG GGGATGCTCT GTGCAGACAC ATGTGCCGGG 
     2401 CCATTGGCGA GAGCTTCATC ATGAAGCGTG GAGACAGCTT CCTCGATGGG ACCCGGTGTA 
     2461 TGCCAAGTGG CCCCCGGGAG GACGGGACCC TGAGCCTGTG TGTGTCGGGC AGCTGCAGGA 
     2521 CATTTGGCTG TGATGGTAGG ATGGACTCCC AGCAGGTATG GGACAGGTGC CAGGTGTGTG 
     2581 GTGGGGACAA CAGCACGTGC AGCCCACGGA AGGGCTCTTT CACAGCTGGC AGAGCGAGAG 
     2641 AATATGTCAC ATTTCTGACA GTTACCCCCA ACCTGACCAG TGTCTACATT GCCAACCACA 
     2701 GGCCTCTCTT CACACACTTG GCGGTGAGGA TCGGAGGGCG CTATGTCGTG GCTGGGAAGA 
     2761 TGAGCATCTC CCCTAACACC ACCTACCCCT CCCTCCTGGA GGATGGTCGT GTCGAGTACA 
     2821 GAGTGGCCCT CACCGAGGAC CGGCTGCCCC GCCTGGAGGA GATCCGCATC TGGGGACCCC 
     2881 TCCAGGAAGA TGCTGACATC CAGGTTTACA GGCGGTATGG CGAGGAGTAT GGCAACCTCA 
     2941 CCCGCCCAGA CATCACCTTC ACCTACTTCC AGCCTAAGCC ACGGCAGGCC TGGGTGTGGG 
     3001 CCGCTGTGCG TGGGCCCTGC TCGGTGAGCT GTGGGGCAGG GCTGCGCTGG GTAAACTACA 
     3061 GCTGCCTGGA CCAGGCCAGG AAGGAGTTGG TGGAGACTGT CCAGTGCCAA GGGAGCCAGC 
     3121 AGCCACCAGC GTGGCCAGAG GCCTGCGTGC TCGAACCCTG CCCTCCCTAC TGGGCGGTGG 
     3181 GAGACTTCGG CCCATGCAGC GCCTCCTGTG GGGGCGGCCT GCGGGAGCGG CCAGTGCGCT 
     3241 GCGTGGAGGC CCAGGGCAGC CTCCTGAAGA CATTGCCCCC AGCCCGGTGC AGAGCAGGGG 
     3301 CCCAGCAGCC AGCTGTGGCG CTGGAAACCT GCAACCCCCA GCCCTGCCCT GCCAGGTGGG 
     3361 AGGTGTCAGA GCCCAGCTCA TGCACATCAG CTGGTGGAGC AGGCCTGGCC TTGGAGAACG 
     3421 AGACCTGTGT GCCAGGGGCA GATGGCCTGG AGGCTCCAGT GACTGAGGGG CCTGGCTCCG 
     3481 TAGATGAGAA GCTGCCTGCC CCTGAGCCCT GTGTCGGGAT GTCATGTCCT CCAGGCTGGG 
     3541 GCCATCTGGA TGCCACCTCT GCAGGGGAGA AGGCTCCCTC CCCATGGGGC AGCATCAGGA 
     3601 CGGGGGCTCA AGCTGCACAC GTGTGGACCC CTGCGGCAGG GTCGTGCTCC GTCTCCTGCG 
     3661 GGCGAGGTCT GATGGAGCTG CGTTTCCTGT GCATGGACTC TGCCCTCAGG GTGCCTGTCC 
     3721 AGGAAGAGCT GTGTGGCCTG GCAAGCAAGC CTGGGAGCCG GCGGGAGGTC TGCCAGGCTG 
     3781 TCCCGTGCCC TGCTCGGTGG CAGTACAAGC TGGCGGCCTG CAGCGTGAGC TGTGGGAGAG 
     3841 GGGTCGTGCG GAGGATCCTG TATTGTGCCC GGGCCCATGG GGAGGACGAT GGTGAGGAGA 
     3901 TCCTGTTGGA CACCCAGTGC CAGGGGCTGC CTCGCCCGGA ACCCCAGGAG GCCTGCAGCC 
     3961 TGGAGCCCTG CCCACCTAGG TGGAAAGTCA TGTCCCTTGG CCCATGTTCG GCCAGCTGTG 
     4021 GCCTTGGCAC TGCTAGACGC TCGGTGGCCT GTGTGCAGCT CGACCAAGGC CAGGACGTGG 
     4081 AGGTGGACGA GGCGGCCTGT GCGGCGCTGG TGCGGCCCGA GGCCAGTGTC CCCTGTCTCA 
     4141 TTGCCGACTG CACCTACCGC TGGCATGTTG GCACCTGGAT GGAGTGCTCT GTTTCCTGTG 
     4201 GGGATGGCAT CCAGCGCCGG CGTGACACCT GCCTCGGACC CCAGGCCCAG GCGCCTGTGC 
     4261 CAGCTGATTT CTGCCAGCAC TTGCCCAAGC CGGTGACTGT GCGTGGCTGC TGGGCTGGGC 
     4321 CCTGTGTGGG ACAGGGTGCC TGTGGCAGGC AGCACCTTGA GCCAACAGGA ACCATTGACA 
     4381 TGCGAGGCCC AGGGCAGGCA GACTGTGCAG TGGCCATTGG GCGGCCCCTC GGGGAGGTGG 
     4441 TGACCCTCCG CGTCCTTGAG AGTTCTCTCA ACTGCAGTGC GGGGGACATG TTGCTGCTTT 
     4501 GGGGCCGGCT CACCTGGAGG AAGATGTGCA GGAAGCTGTT GGACATGACT TTCAGCTCCA 
     4561 AGACCAACAC GCTGGTGGTG AGGCAGCGCT GCGGGCGGCC AGGAGGTGGG GTGCTGCTGC 
     4621 GGTATGGGAG CCAGCTTGCT CCTGAAACCT TCTACAGAGA ATGTGACATG CAGCTCTTTG 
     4681 GGCCCTGGGG TGAAATCGTG AGCCCCTCGC TGAGTCCAGC CACGAGTAAT GCAGGGGGCT 
     4741 GCCGGCTCTT CATTAATGTG GCTCCGCACG CACGGATTGC CATCCATGCC CTGGCCACCA 
     4801 ACATGGGCGC TGGGACCGAG GGAGCCAATG CCAGCTACAT CTTGATCCGG GACACCCACA 
     4861 GCTTGAGGAC CACAGCGTTC CATGGGCAGC AGGTGCTCTA CTGGGAGTCA GAGAGCAGCC 
     4921 AGGCTGAGAT GGAGTTCAGC GAGGGCTTCC TGAAGGCTCA GGCCAGCCTG CGGGGCCAGT 
     4981 ACTGGACCCT CCAATCATGG GTACCGGAGA TGCAGGACCC TCAGTCCTGG AAGGGAAAGG 
     5041 AAGGAACCAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     5101 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     5161 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     5221 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     5281 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     5341 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     5401 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5461 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5521 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5581 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5641 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5701 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5761 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5821 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5881 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5941 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     6001 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     6061 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     6121 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     6181 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     6241 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     6301 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6361 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6421 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6481 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6541 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6601 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6661 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6721 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6781 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6841 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6901 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6961 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     7021 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     7081 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     7141 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     7201 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     7261 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     7321 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7381 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7441 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7501 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7561 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7621 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7681 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7741 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7801 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7861 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7921 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7981 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     8041 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     8101 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     8161 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8221 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8281 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     8341 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8401 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8461 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8521 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8581 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8641 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8701 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8761 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8821 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8881 CAAAAATTTA ACGCGAATTT T
//