LOCUS       RC222033                8181 bp    DNA     circular  SYN 
DEFINITION  TMC3 (Myc-DDK-tagged)-Human transmembrane channel-like 3 (TMC3)
ACCESSION   NM_001080532
VERSION     NM_001080532.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4338..4429 
                     /label="Myc-DDK"  
     misc_feature    4459..5044 
                     /label="PolyA signal"  
     misc_feature    4516..4535 
                     /label="Restriction Enzyme"  
     misc_feature    5320..5992 
                     /label="ColE1"  
     misc_feature    6422..7216 
                     /label="Kan/Neo"  
     misc_feature    7251..7553 
                     /label="SV40 ori"  
     misc_feature    7639..8116 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4320..4326 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4320 
                     /label="TMC3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAAACCTCG AAGGCATCCC AGCGCTATAG AGGCATCCGG AGAAATGCCA 
     1081 GCCAGTGCTA CCTCTACCAG GAATCTCTGC TGCTCAGCAA CTTGGATGAC AGCTTTAGTG 
     1141 CTGATGAAAC AGGGGACAGC AATGATCCGG AACAAATCTT CCAGAATATA CAGTTCCAGA 
     1201 AAGATCTCAT GGCAAACATT CGCTGCAGAC CCTGGACTAT GGGACAGAAG CTGAGGGCAT 
     1261 TGAGACAAGC GAAGAACATT GTGCTGAAGT TTGAAGGGAG GCTGACCAGG ACCCGAGGCT 
     1321 ACCAAGCAGC AGGTGCAGAG CTCTGGCGGA AATTTGCTCG TCTCGCCTGT AACTTTGTGG 
     1381 TCATCTTCAT TCCCTGGGAA ATGAGGATAA AGAAAATCGA GAGTCATTTT GGATCTGGCG 
     1441 TTGCCTCCTA TTTCATATTC TTGAGATGGT TATTTGGAAT TAATATTGTG CTCACCATTA 
     1501 TGACAGGTGC TTTTATTGTC ATTCCAGAGC TGATTGCAGG CCAGCCCTTT GGAAGCACAG 
     1561 CCAGGAAGAC CATCCCCAAG GAGCAGGTTT CGTCTGCCCA AGACCTGGAC ACCGTCTGGT 
     1621 CTCTGGGGGG CTACCTCCAG TACTCTGTCC TCTTCTACGG ATATTACGGC AGGGAGAGGA 
     1681 AGATCGGGAG AGCTGGCTAC CGGCTGCCCT TGGCGTATTT CCTAGTGGGG ATGGCAGTGT 
     1741 TTGCTTACAG CTTCATCATT CTTTTAAAAA AGATGGCTAA GAACTCCCGC ACGAGTCTTG 
     1801 CCAGTGCTTC CAATGAAAAC TATACCTTCT GCTGGCGGGT GTTCTGTGCC TGGGATTACC 
     1861 TCATTGGAAA CCCAGAGGCT GCAGAGAGCA AAACAGCTGC CATAGTGAAC AGCATCAGGG 
     1921 AAGCTATATT GGAAGAACAG GAGAAAAAGA AAAGCAAAAA CCTGGCAGTG ACCATCTGCC 
     1981 TGAGGATTAT TGCCAACATC CTGGTGCTTC TCTCACTGGC TGGGAGCATT TATCTCATCT 
     2041 ACTTTGTGGT GGACCGGTCC CAGAAGCTGG AGCAGTCGAA GAAGGAGCTG ACACTTTGGG 
     2101 AAAAGAATGA GGTCAGTGTG GTGGTCTCCC TCGTCACCAT GATAGCACCA TCAGCCTTTG 
     2161 ACCTCATTGC TGCCTTAGAG ATGTACCACC CCAGGACCAC GCTGCGCTTC CAGCTTGCAA 
     2221 GGGTCCTTGT GCTGTATTTG GGAAATCTCT ACAGCCTGAT CATTGCTCTC CTGGACAAAG 
     2281 TTAACAGCAT GAGCATTGAG GAAATGGCTA CCAAAAATAA CACAAGTCAT TGGATAGATT 
     2341 CCACCACCTT CTTTGCAACC AGGACAGCCC CTGAAGAAGA GAAATGGTCC ACATCTCGGC 
     2401 CTGGAATGGG GCTCAGGAGA AACAACACAT GGGCCTTGGA AGAGACCAGC ATTTCAGCTT 
     2461 ATACCATGCC TCTTATAAAG GCCAACAAGA CTAGCCTCCA CACTCAGAGT CCACAAGACC 
     2521 AGTGCTGGGA GACATACGTT GGTCAGGAGA TGCTGAAGCT CTCCATCATT GACATGCTCT 
     2581 TCACCGTGGC GAGCATTCTG CTCATAGACT TCTTCCGAGG ACTTTTCGTG CGGTACTTAA 
     2641 GTGACTACTG GTGTTGGGAT CTGGAGAGCA AGTTTCCTGA ATATGGAGAA TTCAAAATAG 
     2701 CTGAGAATGT GCTACATTTA GTCTACAACC AAGGAATGAT TTGGATGGGG GCCTTCTTCT 
     2761 CCCCATGTCT CCCAGCGTTC AACGTTCTCA AACTCATTGG GCTCATGTAC CTGAGAAGCT 
     2821 GGGCTGTGCT GACCTGCAAT GTGCCCCACC AGCAAGTATT TCGAGCCTCA AGATCCAACA 
     2881 ACTTCTACTT GGCAATGCTC CTGTTTATGC TGTTTCTGTG CATGCTGCCA ACCATTTTTG 
     2941 CTATTGTCCG ATACAAGCCA TCTCTAAACT GTGGGCCATT CAGTGGACAA GAGAAAATTT 
     3001 ATGACATTGT GTCAGAAACG ATTGAGAAAG ACTTTCCTGT GTGGTTTGGC TCCGTGGTTG 
     3061 GACACATCAG TAGCCCCGTG GTCATCCTGC CCGCAGTACT CCTGCTTTTC ATGCTCATCT 
     3121 ATTATCTCCA GAGCATCGCA CGGTCTCTAA AGCTCAGCAA CCACCAGCTC AAAATGCAGA 
     3181 TCCAAAACGC AAGATCAGAG GATAAGAAAA AGGTTGCCCA GATGGTAGAA GCCCGAATCC 
     3241 AGACCCAGGA AGAAAGCACC AAGAAGCTTC CAAACGACAG TGATCTTACC AGCCAGCTGT 
     3301 CCTCAGCGCA CTCGGGCACA CCCCAAAACA ACGGCAACGT GGCCCATTTT GACTCAGGGA 
     3361 GCAGCAAGAG TGGCAGGATA GAGACAGTCG CACAGTCCAT GCCCCAGAGC CCGAGGCCAG 
     3421 GGGACAGGGC TCCTAGCTCA CCTCTCCCTG GGGTCCCCAA ATCCAGGCTA GAGCATGAAA 
     3481 CTAACAGGTA TCTGCACGGT CTGTGTGCAA GCACCAGTGA CCTTCACAGG AACAGATCGC 
     3541 GCACACCTAT GACATTTACA ACGCACATCG AAGATGTACA CTCAGAACCT CTTTTCCGAA 
     3601 AAGACTTTCA GCAAATCAAC CCTCCTCACC GTGGACCACA GGCCTCGACT TTGCTGGCAC 
     3661 AGGGTCCCAG GCCCCACGCC CCCAGATACT ATGTCATTAA TGAATGTGAC TCTTACAAGA 
     3721 AAAAACATCT AAATGTCTGG CCAGAAAGAC ATTTCAAGAT AGATGCTTCA GGTGACATTG 
     3781 TGGAGCTGTA CCCCAGGAAC GTGCGACAAT ATGCATCCCG GGTCCCCCGC CAGCCTCCCT 
     3841 CCCCACAGCT GAGTGAAGAA GAGGAGGAGA CGCCAAGCAG AGATTGGATC AAACGGTCTC 
     3901 TTCCCCCACG CTCCCTGATA GACCTCCGTC GGGCTCCTCA TTTTTATATT GGGGAGAGGT 
     3961 CCGAAAGTCA GACCCGGGAT CCCGAGCACC AGGGGAGGGT GCACTACAAG TCGTGGAATG 
     4021 AAGATTTTGA GGGTCACCTT GAGAGGCCAG CCTATGTGCC CAGAAAGCCA CGATCCCGGA 
     4081 ATTTCCAATA CCCACAGCCC CCTCTGAAGC CCAGAGGGAA GCCCAGGTTC GAGCCATCCC 
     4141 TCACGGAATC TGACTCCGTG TCGGCAGCAT CCAGCAGTGA CCAGCAGAAC AGCAGCGCTG 
     4201 ACCAGTACCT GCAGGTCACC CACAGCCAGG GCAGGTTCCC GAGGTCCGTG GGCCAGCCCA 
     4261 GCAGGAGGAA GGCCAAGTCG GGGCAGGAGC TGACCGTGGA TCTGGATGAC TTGATTTGTT 
     4321 CAGATGTCAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4381 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4441 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4501 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4561 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4621 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4681 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4741 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4801 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4861 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4921 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4981 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5041 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5101 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5161 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5221 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5281 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5341 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5401 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5461 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5521 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5581 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5641 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5701 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5761 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     5821 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     5881 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     5941 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6001 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6061 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6121 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6181 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6241 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6301 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6361 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6421 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6481 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6541 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6601 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6661 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6721 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6781 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6841 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     6901 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     6961 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7021 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7081 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7141 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7201 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7261 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7321 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7381 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7441 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7501 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7561 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7621 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7681 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7741 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7801 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7861 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     7921 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     7981 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8041 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8101 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8161 CAAAAATTTA ACGCGAATTT T
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