LOCUS       RC222006                8304 bp    DNA     circular  SYN 
DEFINITION  PDE3A (Myc-DDK-tagged)-Human phosphodiesterase 3A, cGMP-inhibited (PDE3A)
ACCESSION   NM_000921
VERSION     NM_000921.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4461..4552 
                     /label="Myc-DDK"  
     misc_feature    4582..5167 
                     /label="PolyA signal"  
     misc_feature    4639..4658 
                     /label="Restriction Enzyme"  
     misc_feature    5443..6115 
                     /label="ColE1"  
     misc_feature    6545..7339 
                     /label="Kan/Neo"  
     misc_feature    7374..7676 
                     /label="SV40 ori"  
     misc_feature    7762..8239 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4443..4449 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4443 
                     /label="PDE3A"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAGTGCCC GGCGACGCTG CACGAGTCAG GGACAAGCCC GTCCACAGTG 
     1081 GGGTGAGTCA AGCCCCCACG GCGGGCCGGG ACTGCCACCA TCGTGCGGAC CCCGCATCGC 
     1141 CGCGGGACTC GGGCTGCCGT GGCTGCTGGG GAGACCTGGT GCTGCAGCCG CTCCGGAGCT 
     1201 CTCGGAAACT TTCCTCCGCG CTGTGCGCGG GCTCCCTGTC CTTTCTGCTG GCGCTGCTGG 
     1261 TGAGGCTGGT CCGCGGGGAG GTCGGCTGTG ACCTGGAGCA GTGTAAGGAG GCGGCGGCGG 
     1321 CGGAGGAGGA GGAAGCAGCC CCGGGAGCAG AAGGGGGCGT CTTCCCGGGG CCTCGGGGAG 
     1381 GTGCTCCCGG GGGCGGTGCG CGGCTCAGCC CCTGGCTGCA GCCCTCGGCG CTGCTCTTCA 
     1441 GTCTCCTGTG TGCCTTCTTC TGGATGGGCT TGTACCTCCT GCGCGCCGGG GTGCGCCTGC 
     1501 CTCTGGCTGT CGCGCTGCTG GCCGCCTGCT GCGGGGGGGA AGCGCTCGTC CAGATTGGGC 
     1561 TGGGCGTCGG GGAGGATCAC TTACTCTCAC TCCCCGCCGC GGGGGTGGTG CTCAGCTGCT 
     1621 TGGCCGCCGC GACATGGCTG GTGCTGAGGC TGAGGCTGGG CGTCCTCATG ATCGCCTTGA 
     1681 CTAGCGCGGT CAGGACCGTG TCCCTCATTT CCTTAGAGAG GTTCAAGGTC GCCTGGAGAC 
     1741 CTTACCTGGC GTACCTGGCC GGCGTGCTGG GGATCCTCTT GGCCAGGTAC GTGGAACAAA 
     1801 TCTTGCCGCA GTCCGCGGAG GCGGCTCCAA GGGAGCATTT GGGGTCCCAG CTGATTGCTG 
     1861 GGACCAAGGA AGATATCCCG GTGTTTAAGA GGAGGAGGCG GTCCAGCTCC GTCGTGTCCG 
     1921 CCGAGATGTC CGGCTGCAGC AGCAAGTCCC ATCGGAGGAC CTCCCTGCCC TGTATACCGA 
     1981 GGGAACAGCT CATGGGGCAT TCAGAATGGG ACCACAAACG AGGGCCAAGA GGATCACAGT 
     2041 CTTCAGGAAC CAGTATTACT GTGGACATCG CCGTCATGGG CGAGGCCCAC GGCCTCATTA 
     2101 CCGACCTCCT GGCAGACCCT TCTCTTCCAC CAAACGTGTG CACATCCTTG AGAGCCGTGA 
     2161 GCAACTTGCT CAGCACACAG CTCACCTTCC AGGCCATTCA CAAGCCCAGA GTGAATCCCG 
     2221 TTACTTCGCT CAGTGAAAAC TATACCTGTT CTGACTCTGA AGAGAGCTCT GAAAAAGACA 
     2281 AGCTTGCTAT TCCAAAGCGC CTGAGAAGGA GTTTGCCTCC TGGCTTGTTG AGACGAGTTT 
     2341 CTTCCACTTG GACCACCACC ACCTCGGCCA CAGGTCTACC CACCTTGGAG CCTGCACCAG 
     2401 TACGGAGAGA CCGCAGCACC AGCATCAAAC TGCAGGAAGC ACCTTCATCC AGTCCTGATT 
     2461 CTTGGAATAA TCCAGTGATG ATGACCCTCA CCAAAAGCAG ATCCTTTACT TCATCCTATG 
     2521 CTATTTCTGC AGCTAACCAT GTAAAGGCTA AAAAGCAAAG TCGACCAGGT GCCCTCGCTA 
     2581 AAATTTCACC TCTTTCATCG CCCTGCTCCT CACCTCTCCA AGGGACTCCT GCCAGCAGCC 
     2641 TGGTCAGCAA AATTTCTGCA GTGCAGTTTC CAGAATCTGC TGACACAACT GCCAAACAAA 
     2701 GCCTAGGTTC TCACAGGGCC TTAACTTACA CTCAGAGTGC CCCAGACCTA TCCCCTCAAA 
     2761 TCCTGACTCC ACCTGTTATA TGTAGCAGCT GTGGCAGACC ATATTCCCAA GGGAATCCTG 
     2821 CTGATGAGCC CCTGGAGAGA AGTGGGGTAG CCACTCGGAC ACCAAGTAGA ACAGATGACA 
     2881 CTGCTCAAGT TACCTCTGAT TATGAAACCA ATAACAACAG TGACAGCAGT GACATTGTAC 
     2941 AGAATGAAGA TGAAACAGAG TGCCTGAGAG AGCCTCTGAG GAAAGCATCG GCTTGCAGCA 
     3001 CCTATGCTCC TGAGACCATG ATGTTTCTGG ACAAACCAAT TCTTGCTCCC GAACCTCTTG 
     3061 TCATGGATAA CCTGGACTCA ATTATGGAGC AGCTAAATAC TTGGAATTTT CCAATTTTTG 
     3121 ATTTAGTGGA AAATATAGGA AGAAAATGTG GCCGTATTCT TAGTCAGGTA TCTTACAGAC 
     3181 TTTTTGAAGA CATGGGCCTG TTTGAAGCTT TTAAAATTCC AATTAGGGAA TTTATGAATT 
     3241 ATTTTCATGC TTTGGAGATT GGATATAGGG ATATTCCTTA TCATAACAGA ATCCATGCCA 
     3301 CTGATGTTTT ACATGCTGTT TGGTATCTTA CTACACAGCC TATTCCAGGC CTCTCAACTG 
     3361 TGATTAATGA TCATGGTTCA ACCAGTGATT CAGATTCTGA CAGTGGATTT ACACATGGAC 
     3421 ATATGGGATA TGTATTCTCA AAAACGTATA ATGTGACAGA TGATAAATAC GGATGTCTGT 
     3481 CTGGGAATAT CCCTGCCTTG GAGTTGATGG CGCTGTATGT GGCTGCAGCC ATGCACGATT 
     3541 ATGATCATCC AGGAAGGACT AATGCTTTCC TGGTTGCAAC TAGTGCTCCT CAGGCGGTGC 
     3601 TATATAACGA TCGTTCAGTT TTGGAGAATC ATCACGCAGC TGCTGCATGG AATCTTTTCA 
     3661 TGTCCCGGCC AGAGTATAAC TTCTTAATTA ACCTTGACCA TGTGGAATTT AAGCATTTCC 
     3721 GTTTCCTTGT CATTGAAGCA ATTTTGGCCA CTGACCTGAA GAAACACTTT GACTTCGTAG 
     3781 CCAAATTTAA TGGCAAGGTA AATGATGATG TTGGAATAGA TTGGACCAAT GAAAATGATC 
     3841 GTCTACTGGT TTGTCAAATG TGTATAAAGT TGGCTGATAT CAATGGTCCA GCTAAATGTA 
     3901 AAGAACTCCA TCTTCAGTGG ACAGATGGTA TTGTCAATGA ATTTTATGAA CAGGGTGATG 
     3961 AAGAGGCCAG CCTTGGATTA CCCATAAGCC CCTTCATGGA TCGTTCTGCT CCTCAGCTGG 
     4021 CCAACCTTCA GGAATCCTTC ATCTCTCACA TTGTGGGGCC TCTGTGCAAC TCCTATGATT 
     4081 CAGCAGGACT AATGCCTGGA AAATGGGTGG AAGACAGCGA TGAGTCAGGA GATACTGATG 
     4141 ACCCAGAAGA AGAGGAGGAA GAAGCACCAG CACCAAATGA AGAGGAAACC TGTGAAAATA 
     4201 ATGAATCTCC AAAAAAGAAG ACTTTCAAAA GGAGAAAAAT CTACTGCCAA ATAACTCAGC 
     4261 ACCTCTTACA GAACCACAAG ATGTGGAAGA AAGTCATTGA AGAGGAGCAA CGGTTGGCAG 
     4321 GCATAGAAAA TCAATCCCTG GACCAGACCC CTCAGTCGCA CTCTTCAGAA CAGATCCAGG 
     4381 CTATCAAGGA AGAAGAAGAA GAGAAAGGGA AACCAAGAGG CGAGGAGATA CCAACCCAAA 
     4441 AGCCAGACCA GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4501 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4561 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4621 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4681 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4741 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4801 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4861 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4921 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4981 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5041 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5101 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5161 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5221 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5281 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5341 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5401 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5461 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5521 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5581 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5641 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5701 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5761 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5821 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5881 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5941 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6001 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6061 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6121 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6181 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6241 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6301 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6361 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6421 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6481 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6541 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6601 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6661 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6721 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6781 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6841 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6901 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6961 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7021 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7081 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7141 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7201 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7261 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7321 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7381 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7441 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7501 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7561 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7621 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7681 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7741 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7801 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7861 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7921 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7981 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8041 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8101 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8161 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8221 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8281 TAACAAAAAT TTAACGCGAA TTTT
//