LOCUS       RC221990                8100 bp    DNA     circular  SYN 
DEFINITION  ITGA6 (Myc-DDK-tagged)-Human integrin, alpha 6 (ITGA6), transcript variant 2
ACCESSION   NM_000210
VERSION     NM_000210.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4257..4348 
                     /label="Myc-DDK"  
     misc_feature    4378..4963 
                     /label="PolyA signal"  
     misc_feature    4435..4454 
                     /label="Restriction Enzyme"  
     misc_feature    5239..5911 
                     /label="ColE1"  
     misc_feature    6341..7135 
                     /label="Kan/Neo"  
     misc_feature    7170..7472 
                     /label="SV40 ori"  
     misc_feature    7558..8035 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4239..4245 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4239 
                     /label="ITGA6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCGCCGCC GGGCAGCTGT GCTTGCTCTA CCTGTCGGCG GGGCTCCTGT 
     1081 CCCGGCTCGG CGCAGCCTTC AACTTGGACA CTCGGGAGGA CAACGTGATC CGGAAATATG 
     1141 GAGACCCCGG GAGCCTCTTC GGCTTCTCGC TGGCCATGCA CTGGCAACTG CAGCCCGAGG 
     1201 ACAAGCGGCT GTTGCTCGTG GGGGCCCCGC GGGCAGAAGC GCTTCCACTG CAGAGAGCCA 
     1261 ACAGAACGGG AGGGCTGTAC AGCTGCGACA TCACCGCCCG GGGGCCATGC ACGCGGATCG 
     1321 AGTTTGATAA CGATGCTGAC CCCACGTCAG AAAGCAAGGA AGATCAGTGG ATGGGGGTCA 
     1381 CCGTCCAGAG CCAAGGTCCA GGGGGCAAGG TCGTGACATG TGCTCACCGA TATGAAAAAA 
     1441 GGCAGCATGT TAATACGAAG CAGGAATCCC GAGACATCTT TGGGCGGTGT TATGTCCTGA 
     1501 GTCAGAATCT CAGGATTGAA GACGATATGG ATGGGGGAGA TTGGAGCTTT TGTGATGGGC 
     1561 GATTGAGAGG CCATGAGAAA TTTGGCTCTT GCCAGCAAGG TGTAGCAGCT ACTTTTACTA 
     1621 AAGACTTTCA TTACATTGTA TTTGGAGCCC CGGGTACTTA TAACTGGAAA GGGATTGTTC 
     1681 GTGTAGAGCA AAAGAATAAC ACTTTTTTTG ACATGAACAT CTTTGAAGAT GGGCCTTATG 
     1741 AAGTTGGTGG AGAGACTGAG CATGATGAAA GTCTCGTTCC TGTTCCTGCT AACAGTTACT 
     1801 TAGGTTTTTC TTTGGACTCA GGGAAAGGTA TTGTTTCTAA AGATGAGATC ACTTTTGTAT 
     1861 CTGGTGCTCC CAGAGCCAAT CACAGTGGAG CCGTGGTTTT GCTGAAGAGA GACATGAAGT 
     1921 CTGCACATCT CCTCCCTGAG CACATATTCG ATGGAGAAGG TCTGGCCTCT TCATTTGGCT 
     1981 ATGATGTGGC GGTGGTGGAC CTCAACAAGG ATGGGTGGCA AGATATAGTT ATTGGAGCCC 
     2041 CACAGTATTT TGATAGAGAT GGAGAAGTTG GAGGTGCAGT GTATGTCTAC ATGAACCAGC 
     2101 AAGGCAGATG GAATAATGTG AAGCCAATTC GTCTTAATGG AACCAAAGAT TCTATGTTTG 
     2161 GCATTGCAGT AAAAAATATT GGAGATATTA ATCAAGATGG CTACCCAGAT ATTGCAGTTG 
     2221 GAGCTCCGTA TGATGACTTG GGAAAGGTTT TTATCTATCA TGGATCTGCA AATGGAATAA 
     2281 ATACCAAACC AACACAGGTT CTCAAGGGTA TATCACCTTA TTTTGGATAT TCAATTGCTG 
     2341 GAAACATGGA CCTTGATCGA AATTCCTACC CTGATGTTGC TGTTGGTTCC CTCTCAGATT 
     2401 CAGTAACTAT TTTCAGATCC CGGCCTGTGA TTAATATTCA GAAAACCATC ACAGTAACTC 
     2461 CTAACAGAAT TGACCTCCGC CAGAAAACAG CGTGTGGGGC GCCTAGTGGG ATATGCCTCC 
     2521 AGGTTAAATC CTGTTTTGAA TATACTGCTA ACCCCGCTGG TTATAATCCT TCAATATCAA 
     2581 TTGTGGGCAC ACTTGAAGCT GAAAAAGAAA GAAGAAAATC TGGGCTATCC TCAAGAGTTC 
     2641 AGTTTCGAAA CCAAGGTTCT GAGCCCAAAT ATACTCAAGA ACTAACTCTG AAGAGGCAGA 
     2701 AACAGAAAGT GTGCATGGAG GAAACCCTGT GGCTACAGGA TAATATCAGA GATAAACTGC 
     2761 GTCCCATTCC CATAACTGCC TCAGTGGAGA TCCAAGAGCC AAGCTCTCGT AGGCGAGTGA 
     2821 ATTCACTTCC AGAAGTTCTT CCAATTCTGA ATTCAGATGA ACCCAAGACA GCTCATATTG 
     2881 ATGTTCACTT CTTAAAAGAG GGATGTGGAG ACGACAATGT ATGTAACAGC AACCTTAAAC 
     2941 TAGAATATAA ATTTTGCACC CGAGAAGGAA ATCAAGACAA ATTTTCTTAT TTACCAATTC 
     3001 AAAAAGGTGT ACCAGAACTA GTTCTAAAAG ATCAGAAGGA TATTGCTTTA GAAATAACAG 
     3061 TGACAAACAG CCCTTCCAAC CCAAGGAATC CCACAAAAGA TGGCGATGAC GCCCATGAGG 
     3121 CTAAACTGAT TGCAACGTTT CCAGACACTT TAACCTATTC TGCATATAGA GAACTGAGGG 
     3181 CTTTCCCTGA GAAACAGTTG AGTTGTGTTG CCAACCAGAA TGGCTCGCAA GCTGACTGTG 
     3241 AGCTCGGAAA TCCTTTTAAA AGAAATTCAA ATGTCACTTT TTATTTGGTT TTAAGTACAA 
     3301 CTGAAGTCAC CTTTGACACC CCAGATCTGG ATATTAATCT GAAGTTAGAA ACAACAAGCA 
     3361 ATCAAGATAA TTTGGCTCCA ATTACAGCTA AAGCAAAAGT GGTTATTGAA CTGCTTTTAT 
     3421 CGGTCTCGGG AGTTGCTAAA CCTTCCCAGG TGTATTTTGG AGGTACAGTT GTTGGCGAGC 
     3481 AAGCTATGAA ATCTGAAGAT GAAGTGGGAA GTTTAATAGA GTATGAATTC AGGGTAATAA 
     3541 ACTTAGGTAA ACCTCTTACA AACCTCGGCA CAGCAACCTT GAACATTCAG TGGCCAAAAG 
     3601 AAATTAGCAA TGGGAAATGG TTGCTTTATT TGGTGAAAGT AGAATCCAAA GGATTGGAAA 
     3661 AGGTAACTTG TGAGCCACAA AAGGAGATAA ACTCCCTGAA CCTAACGGAG TCTCACAACT 
     3721 CAAGAAAGAA ACGGGAAATT ACTGAAAAAC AGATAGATGA TAACAGAAAA TTTTCTTTAT 
     3781 TTGCTGAAAG AAAATACCAG ACTCTTAACT GTAGCGTGAA CGTGAACTGT GTGAACATCA 
     3841 GATGCCCGCT GCGGGGGCTG GACAGCAAGG CGTCTCTTAT TTTGCGCTCG AGGTTATGGA 
     3901 ACAGCACATT TCTAGAGGAA TATTCCAAAC TGAACTACTT GGACATTCTC ATGCGAGCCT 
     3961 TCATTGATGT GACTGCTGCT GCCGAAAATA TCAGGCTGCC AAATGCAGGC ACTCAGGTTC 
     4021 GAGTGACTGT GTTTCCCTCA AAGACTGTAG CTCAGTATTC GGGAGTACCT TGGTGGATCA 
     4081 TCCTAGTGGC TATTCTCGCT GGGATCTTGA TGCTTGCTTT ATTAGTGTTT ATACTATGGA 
     4141 AGTGTGGTTT CTTCAAGAGA AATAAGAAAG ATCATTATGA TGCCACATAT CACAAGGCTG 
     4201 AGATCCATGC TCAGCCATCT GATAAAGAGA GGCTTACTTC TGATGCAACG CGTACGCGGC 
     4261 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4321 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4381 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4441 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4501 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4561 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4621 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4681 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4741 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4801 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4861 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4921 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4981 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5041 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5101 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5161 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5221 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5281 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5341 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5401 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5461 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5521 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5581 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5641 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5701 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5761 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     5821 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     5881 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     5941 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6001 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6061 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6121 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6181 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6241 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6301 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6361 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6421 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6481 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6541 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6601 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6661 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6721 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6781 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6841 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6901 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     6961 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7021 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7081 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7141 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7201 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7261 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7321 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7381 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7441 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7501 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7561 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7621 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7681 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7741 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7801 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7861 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7921 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     7981 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8041 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//