LOCUS       RC221986L1                9498 bp    DNA     circular  SYN 
DEFINITION  TLR8 (Myc-DDK-tagged)-Human toll-like receptor 8 (TLR8)
ACCESSION   NM_138636
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7789..7969 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8697..9356 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    8128..8696 
                     /label="ColE1"  
     misc_feature    6664..6684 
                     /label="LR50 primer"  
     misc_feature    6461..6532 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6673..7264 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6443..6449 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6443 
                     /label="TLR8"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGAAAACATG TTCCTTCAGT CGTCAATGCT 
     3361 GACCTGCATT TTCCTGCTAA TATCTGGTTC CTGTGAGTTA TGCGCCGAAG AAAATTTTTC 
     3421 TAGAAGCTAT CCTTGTGATG AGAAAAAGCA AAATGACTCA GTTATTGCAG AGTGCAGCAA 
     3481 TCGTCGACTA CAGGAAGTTC CCCAAACGGT GGGCAAATAT GTGACAGAAC TAGACCTGTC 
     3541 TGATAATTTC ATCACACACA TAACGAATGA ATCATTTCAA GGGCTGCAAA ATCTCACTAA 
     3601 AATAAATCTA AACCACAACC CCAATGTACA GCACCAGAAC GGAAATCCCG GTATACAATC 
     3661 AAATGGCTTG AATATCACAG ACGGGGCATT CCTCAACCTA AAAAACCTAA GGGAGTTACT 
     3721 GCTTGAAGAC AACCAGTTAC CCCAAATACC CTCTGGTTTG CCAGAGTCTT TGACAGAACT 
     3781 TAGTCTAATT CAAAACAATA TATACAACAT AACTAAAGAG GGCATTTCAA GACTTATAAA 
     3841 CTTGAAAAAT CTCTATTTGG CCTGGAACTG CTATTTTAAC AAAGTTTGCG AGAAAACTAA 
     3901 CATAGAAGAT GGAGTATTTG AAACGCTGAC AAATTTGGAG TTGCTATCAC TATCTTTCAA 
     3961 TTCTCTTTCA CACGTGCCAC CCAAACTGCC AAGCTCCCTA CGCAAACTTT TTCTGAGCAA 
     4021 CACCCAGATC AAATACATTA GTGAAGAAGA TTTCAAGGGA TTGATAAATT TAACATTACT 
     4081 AGATTTAAGC GGGAACTGTC CGAGGTGCTT CAATGCCCCA TTTCCATGCG TGCCTTGTGA 
     4141 TGGTGGTGCT TCAATTAATA TAGATCGTTT TGCTTTTCAA AACTTGACCC AACTTCGATA 
     4201 CCTAAACCTC TCTAGCACTT CCCTCAGGAA GATTAATGCT GCCTGGTTTA AAAATATGCC 
     4261 TCATCTGAAG GTGCTGGATC TTGAATTCAA CTATTTAGTG GGAGAAATAG CCTCTGGGGC 
     4321 ATTTTTAACG ATGCTGCCCC GCTTAGAAAT ACTTGACTTG TCTTTTAACT ATATAAAGGG 
     4381 GAGTTATCCA CAGCATATTA ATATTTCCAG AAACTTCTCT AAACTTTTGT CTCTACGGGC 
     4441 ATTGCATTTA AGAGGTTATG TGTTCCAGGA ACTCAGAGAA GATGATTTCC AGCCCCTGAT 
     4501 GCAGCTTCCA AACTTATCGA CTATCAACTT GGGTATTAAT TTTATTAAGC AAATCGATTT 
     4561 CAAACTTTTC CAAAATTTCT CCAATCTGGA AATTATTTAC TTGTCAGAAA ACAGAATATC 
     4621 ACCGTTGGTA AAAGATACCC GGCAGAGTTA TGCAAATAGT TCCTCTTTTC AACGTCATAT 
     4681 CCGGAAACGA CGCTCAACAG ATTTTGAGTT TGACCCACAT TCGAACTTTT ATCATTTCAC 
     4741 CCGTCCTTTA ATAAAGCCAC AATGTGCTGC TTATGGAAAA GCCTTAGATT TAAGCCTCAA 
     4801 CAGTATTTTC TTCATTGGGC CAAACCAATT TGAAAATCTT CCTGACATTG CCTGTTTAAA 
     4861 TCTGTCTGCA AATAGCAATG CTCAAGTGTT AAGTGGAACT GAATTTTCAG CCATTCCTCA 
     4921 TGTCAAATAT TTGGATTTGA CAAACAATAG ACTAGACTTT GATAATGCTA GTGCTCTTAC 
     4981 TGAATTGTCC GACTTGGAAG TTCTAGATCT CAGCTATAAT TCACACTATT TCAGAATAGC 
     5041 AGGCGTAACA CATCATCTAG AATTTATTCA AAATTTCACA AATCTAAAAG TTTTAAACTT 
     5101 GAGCCACAAC AACATTTATA CTTTAACAGA TAAGTATAAC CTGGAAAGCA AGTCCCTGGT 
     5161 AGAATTAGTT TTCAGTGGCA ATCGCCTTGA CATTTTGTGG AATGATGATG ACAACAGGTA 
     5221 TATCTCCATT TTCAAAGGTC TCAAGAATCT GACACGTCTG GATTTATCCC TTAATAGGCT 
     5281 GAAGCACATC CCAAATGAAG CATTCCTTAA TTTGCCAGCG AGTCTCACTG AACTACATAT 
     5341 AAATGATAAT ATGTTAAAGT TTTTTAACTG GACATTACTC CAGCAGTTTC CTCGTCTCGA 
     5401 GTTGCTTGAC TTACGTGGAA ACAAACTACT CTTTTTAACT GATAGCCTAT CTGACTTTAC 
     5461 ATCTTCCCTT CGGACACTGC TGCTGAGTCA TAACAGGATT TCCCACCTAC CCTCTGGCTT 
     5521 TCTTTCTGAA GTCAGTAGTC TGAAGCACCT CGATTTAAGT TCCAATCTGC TAAAAACAAT 
     5581 CAACAAATCC GCACTTGAAA CTAAGACCAC CACCAAATTA TCTATGTTGG AACTACACGG 
     5641 AAACCCCTTT GAATGCACCT GTGACATTGG AGATTTCCGA AGATGGATGG ATGAACATCT 
     5701 GAATGTCAAA ATTCCCAGAC TGGTAGATGT CATTTGTGCC AGTCCTGGGG ATCAAAGAGG 
     5761 GAAGAGTATT GTGAGTCTGG AGCTAACAAC TTGTGTTTCA GATGTCACTG CAGTGATATT 
     5821 ATTTTTCTTC ACGTTCTTTA TCACCACCAT GGTTATGTTG GCTGCCCTGG CTCACCATTT 
     5881 GTTTTACTGG GATGTTTGGT TTATATATAA TGTGTGTTTA GCTAAGGTAA AAGGCTACAG 
     5941 GTCTCTTTCC ACATCCCAAA CTTTCTATGA TGCTTACATT TCTTATGACA CCAAAGATGC 
     6001 CTCTGTTACT GACTGGGTGA TAAATGAGCT GCGCTACCAC CTTGAAGAGA GCCGAGACAA 
     6061 AAACGTTCTC CTTTGTCTAG AGGAGAGGGA TTGGGATCCG GGATTGGCCA TCATCGACAA 
     6121 CCTCATGCAG AGCATCAACC AAAGCAAGAA AACAGTATTT GTTTTAACCA AAAAATATGC 
     6181 AAAAAGCTGG AACTTTAAAA CAGCTTTTTA CTTGGCTTTG CAGAGGCTAA TGGATGAGAA 
     6241 CATGGATGTG ATTATATTTA TCCTGCTGGA GCCAGTGTTA CAGCATTCTC AGTATTTGAG 
     6301 GCTACGGCAG CGGATCTGTA AGAGCTCCAT CCTCCAGTGG CCTGACAACC CGAAGGCAGA 
     6361 AGGCTTGTTT TGGCAAACTC TGAGAAATGT GGTCTTGACT GAAAATGATT CACGGTATAA 
     6421 CAATATGTAT GTCGATTCCA TTAAGCAATA CACGCGTACG CGGCCGCTCG AGCAGAAACT 
     6481 CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA 
     6541 GGTTTAAACG GCCGGCCGCG GTCTGTACAA GTAGGATTCG TCGAGGGACC TAATAACTTC 
     6601 GTATAGCATA CATTATACGA AGTTATACAT GTTTAAGGGT TCCGGTTCCA CTAGGTACAA 
     6661 TTCGATATCA AGCTTATCGA TAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT 
     6721 GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG 
     6781 TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTG 
     6841 CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT GTGCACTGTG 
     6901 TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA CCTGTCAGCT CCTTTCCGGG 
     6961 ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA TCGCCGCCTG CCTTGCCCGC 
     7021 TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG TGGTGTTGTC GGGGAAATCA 
     7081 TCGTCCTTTC CTTGGCTGCT CGCCTGTGTT GCCACCTGGA TTCTGCGCGG GACGTCCTTC 
     7141 TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT CCCGCGGCCT GCTGCCGGCT 
     7201 CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC CCTCAGACGA GTCGGATCTC CCTTTGGGCC 
     7261 GCCTCCCCGC ATCGATACCG TCGACCTCGA TCGAGACCTA GAAAAACATG GAGCAATCAC 
     7321 AAGTAGCAAT ACAGCAGCTA CCAATGCTGA TTGTGCCTGG CTAGAAGCAC AAGAGGAGGA 
     7381 GGAGGTGGGT TTTCCAGTCA CACCTCAGGT ACCTTTAAGA CCAATGACTT ACAAGGCAGC 
     7441 TGTAGATCTT AGCCACTTTT TAAAAGAAAA GGGGGGACTG GAAGGGCTAA TTCACTCCCA 
     7501 ACGAAGACAA GATATCCTTG ATCTGTGGAT CTACCACACA CAAGGCTACT TCCCTGATTG 
     7561 GCAGAACTAC ACACCAGGGC CAGGGATCAG ATATCCACTG ACCTTTGGAT GGTGCTACAA 
     7621 GCTAGTACCA GTTGAGCAAG AGAAGGTAGA AGAAGCCAAT GAAGGAGAGA ACACCCGCTT 
     7681 GTTACACCCT GTGAGCCTGC ATGGGATGGA TGACCCGGAG AGAGAAGTAT TAGAGTGGAG 
     7741 GTTTGACAGC CGCCTAGCAT TTCATCACAT GGCCCGAGAG CTGCATCCGG ACTGTACTGG 
     7801 GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG GGAACCCACT 
     7861 GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG 
     7921 TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG 
     7981 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     8041 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     8101 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     8161 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     8221 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     8281 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     8341 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     8401 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     8461 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     8521 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     8581 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     8641 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     8701 CATGATTACG CCCCGCCCTG CCACTCATCG CAGTACTGTT GTAATTCATT AAGCATTCTG 
     8761 CCGACATGGA AGCCATCACA AACGGCATGA TGAACCTGAA TCGCCAGCGG CATCAGCACC 
     8821 TTGTCGCCTT GCGTATAATA TTTGCCCATG GTGAAAACGG GGGCGAAGAA GTTGTCCATA 
     8881 TTGGCCACGT TTAAATCAAA ACTGGTGAAA CTCACCCAGG GATTGGCTGA GACGAAAAAC 
     8941 ATATTCTCAA TAAACCCTTT AGGGAAATAG GCCAGGTTTT CACCGTAACA CGCCACATCT 
     9001 TGCGAATATA TGTGTAGAAA CTGCCGGAAA TCGTCGTGGT ATTCACTCCA GAGCGATGAA 
     9061 AACGTTTCAG TTTGCTCATG GAAAACGGTG TAACAAGGGT GAACACTATC CCATATCACC 
     9121 AGCTCACCGT CTTTCATTGC CATACGGAAC TCCGGATGAG CATTCATCAG GCGGGCAAGA 
     9181 ATGTGAATAA AGGCCGGATA AAACTTGTGC TTATTTTTCT TTACGGTCTT TAAAAAGGCC 
     9241 GTAATATCCA GCTGAACGGT CTGGTTATAG GTACATTGAG CAACTGACTG AAATGCCTCA 
     9301 AAATGTTCTT TACGATGCCA TTGGGATATA TCAACGGTGG TATATCCAGT GATTTTTTTC 
     9361 TCCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     9421 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTCCCGCG CACATTTCCC 
     9481 CGAAAAGTGC CACCTGAC
//