LOCUS       RC221986                8004 bp    DNA     circular  SYN 
DEFINITION  TLR8 (Myc-DDK-tagged)-Human toll-like receptor 8 (TLR8)
ACCESSION   NM_138636
VERSION     NM_138636.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4161..4252 
                     /label="Myc-DDK"  
     misc_feature    4282..4867 
                     /label="PolyA signal"  
     misc_feature    4339..4358 
                     /label="Restriction Enzyme"  
     misc_feature    5143..5815 
                     /label="ColE1"  
     misc_feature    6245..7039 
                     /label="Kan/Neo"  
     misc_feature    7074..7376 
                     /label="SV40 ori"  
     misc_feature    7462..7939 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4143..4149 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4143 
                     /label="TLR8"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAAAACATG TTCCTTCAGT CGTCAATGCT GACCTGCATT TTCCTGCTAA 
     1081 TATCTGGTTC CTGTGAGTTA TGCGCCGAAG AAAATTTTTC TAGAAGCTAT CCTTGTGATG 
     1141 AGAAAAAGCA AAATGACTCA GTTATTGCAG AGTGCAGCAA TCGTCGACTA CAGGAAGTTC 
     1201 CCCAAACGGT GGGCAAATAT GTGACAGAAC TAGACCTGTC TGATAATTTC ATCACACACA 
     1261 TAACGAATGA ATCATTTCAA GGGCTGCAAA ATCTCACTAA AATAAATCTA AACCACAACC 
     1321 CCAATGTACA GCACCAGAAC GGAAATCCCG GTATACAATC AAATGGCTTG AATATCACAG 
     1381 ACGGGGCATT CCTCAACCTA AAAAACCTAA GGGAGTTACT GCTTGAAGAC AACCAGTTAC 
     1441 CCCAAATACC CTCTGGTTTG CCAGAGTCTT TGACAGAACT TAGTCTAATT CAAAACAATA 
     1501 TATACAACAT AACTAAAGAG GGCATTTCAA GACTTATAAA CTTGAAAAAT CTCTATTTGG 
     1561 CCTGGAACTG CTATTTTAAC AAAGTTTGCG AGAAAACTAA CATAGAAGAT GGAGTATTTG 
     1621 AAACGCTGAC AAATTTGGAG TTGCTATCAC TATCTTTCAA TTCTCTTTCA CACGTGCCAC 
     1681 CCAAACTGCC AAGCTCCCTA CGCAAACTTT TTCTGAGCAA CACCCAGATC AAATACATTA 
     1741 GTGAAGAAGA TTTCAAGGGA TTGATAAATT TAACATTACT AGATTTAAGC GGGAACTGTC 
     1801 CGAGGTGCTT CAATGCCCCA TTTCCATGCG TGCCTTGTGA TGGTGGTGCT TCAATTAATA 
     1861 TAGATCGTTT TGCTTTTCAA AACTTGACCC AACTTCGATA CCTAAACCTC TCTAGCACTT 
     1921 CCCTCAGGAA GATTAATGCT GCCTGGTTTA AAAATATGCC TCATCTGAAG GTGCTGGATC 
     1981 TTGAATTCAA CTATTTAGTG GGAGAAATAG CCTCTGGGGC ATTTTTAACG ATGCTGCCCC 
     2041 GCTTAGAAAT ACTTGACTTG TCTTTTAACT ATATAAAGGG GAGTTATCCA CAGCATATTA 
     2101 ATATTTCCAG AAACTTCTCT AAACTTTTGT CTCTACGGGC ATTGCATTTA AGAGGTTATG 
     2161 TGTTCCAGGA ACTCAGAGAA GATGATTTCC AGCCCCTGAT GCAGCTTCCA AACTTATCGA 
     2221 CTATCAACTT GGGTATTAAT TTTATTAAGC AAATCGATTT CAAACTTTTC CAAAATTTCT 
     2281 CCAATCTGGA AATTATTTAC TTGTCAGAAA ACAGAATATC ACCGTTGGTA AAAGATACCC 
     2341 GGCAGAGTTA TGCAAATAGT TCCTCTTTTC AACGTCATAT CCGGAAACGA CGCTCAACAG 
     2401 ATTTTGAGTT TGACCCACAT TCGAACTTTT ATCATTTCAC CCGTCCTTTA ATAAAGCCAC 
     2461 AATGTGCTGC TTATGGAAAA GCCTTAGATT TAAGCCTCAA CAGTATTTTC TTCATTGGGC 
     2521 CAAACCAATT TGAAAATCTT CCTGACATTG CCTGTTTAAA TCTGTCTGCA AATAGCAATG 
     2581 CTCAAGTGTT AAGTGGAACT GAATTTTCAG CCATTCCTCA TGTCAAATAT TTGGATTTGA 
     2641 CAAACAATAG ACTAGACTTT GATAATGCTA GTGCTCTTAC TGAATTGTCC GACTTGGAAG 
     2701 TTCTAGATCT CAGCTATAAT TCACACTATT TCAGAATAGC AGGCGTAACA CATCATCTAG 
     2761 AATTTATTCA AAATTTCACA AATCTAAAAG TTTTAAACTT GAGCCACAAC AACATTTATA 
     2821 CTTTAACAGA TAAGTATAAC CTGGAAAGCA AGTCCCTGGT AGAATTAGTT TTCAGTGGCA 
     2881 ATCGCCTTGA CATTTTGTGG AATGATGATG ACAACAGGTA TATCTCCATT TTCAAAGGTC 
     2941 TCAAGAATCT GACACGTCTG GATTTATCCC TTAATAGGCT GAAGCACATC CCAAATGAAG 
     3001 CATTCCTTAA TTTGCCAGCG AGTCTCACTG AACTACATAT AAATGATAAT ATGTTAAAGT 
     3061 TTTTTAACTG GACATTACTC CAGCAGTTTC CTCGTCTCGA GTTGCTTGAC TTACGTGGAA 
     3121 ACAAACTACT CTTTTTAACT GATAGCCTAT CTGACTTTAC ATCTTCCCTT CGGACACTGC 
     3181 TGCTGAGTCA TAACAGGATT TCCCACCTAC CCTCTGGCTT TCTTTCTGAA GTCAGTAGTC 
     3241 TGAAGCACCT CGATTTAAGT TCCAATCTGC TAAAAACAAT CAACAAATCC GCACTTGAAA 
     3301 CTAAGACCAC CACCAAATTA TCTATGTTGG AACTACACGG AAACCCCTTT GAATGCACCT 
     3361 GTGACATTGG AGATTTCCGA AGATGGATGG ATGAACATCT GAATGTCAAA ATTCCCAGAC 
     3421 TGGTAGATGT CATTTGTGCC AGTCCTGGGG ATCAAAGAGG GAAGAGTATT GTGAGTCTGG 
     3481 AGCTAACAAC TTGTGTTTCA GATGTCACTG CAGTGATATT ATTTTTCTTC ACGTTCTTTA 
     3541 TCACCACCAT GGTTATGTTG GCTGCCCTGG CTCACCATTT GTTTTACTGG GATGTTTGGT 
     3601 TTATATATAA TGTGTGTTTA GCTAAGGTAA AAGGCTACAG GTCTCTTTCC ACATCCCAAA 
     3661 CTTTCTATGA TGCTTACATT TCTTATGACA CCAAAGATGC CTCTGTTACT GACTGGGTGA 
     3721 TAAATGAGCT GCGCTACCAC CTTGAAGAGA GCCGAGACAA AAACGTTCTC CTTTGTCTAG 
     3781 AGGAGAGGGA TTGGGATCCG GGATTGGCCA TCATCGACAA CCTCATGCAG AGCATCAACC 
     3841 AAAGCAAGAA AACAGTATTT GTTTTAACCA AAAAATATGC AAAAAGCTGG AACTTTAAAA 
     3901 CAGCTTTTTA CTTGGCTTTG CAGAGGCTAA TGGATGAGAA CATGGATGTG ATTATATTTA 
     3961 TCCTGCTGGA GCCAGTGTTA CAGCATTCTC AGTATTTGAG GCTACGGCAG CGGATCTGTA 
     4021 AGAGCTCCAT CCTCCAGTGG CCTGACAACC CGAAGGCAGA AGGCTTGTTT TGGCAAACTC 
     4081 TGAGAAATGT GGTCTTGACT GAAAATGATT CACGGTATAA CAATATGTAT GTCGATTCCA 
     4141 TTAAGCAATA CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4201 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4261 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4321 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4381 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4441 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4501 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4561 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4621 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4681 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4741 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4801 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4861 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4921 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4981 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5041 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5101 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5161 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5221 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5281 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5341 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5401 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5461 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5521 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5581 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5641 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5701 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5761 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5821 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5881 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     5941 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6001 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6061 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6121 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6181 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6241 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6301 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6361 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6421 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6481 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6541 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6601 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6661 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6721 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6781 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6841 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6901 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6961 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7021 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7081 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7141 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7201 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7261 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7321 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7381 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7441 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7501 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7561 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7621 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7681 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7741 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7801 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7861 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7921 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7981 TAACAAAAAT TTAACGCGAA TTTT
//