LOCUS       RC221938                7935 bp    DNA     circular  SYN 
DEFINITION  PTPRS (Myc-DDK-tagged)-Human protein tyrosine phosphatase, receptor type, S (PTPRS), transcript variant 4
ACCESSION   NM_130855
VERSION     NM_130855.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4090..4181 
                     /label="Myc-DDK"  
     misc_feature    4211..4796 
                     /label="PolyA signal"  
     misc_feature    4268..4287 
                     /label="Restriction Enzyme"  
     misc_feature    5072..5744 
                     /label="ColE1"  
     misc_feature    6174..6968 
                     /label="Kan/Neo"  
     misc_feature    7003..7305 
                     /label="SV40 ori"  
     misc_feature    7391..7868 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4065..4072 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4065 
                     /label="PTPRS"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGCCGCCG CTCCCGCTGC TGCTGCTGCT ACTGCTGCTG CTGCCGCCAC 
     1081 GCGTCCTGCC TGCCGCCCCT TCGTCCGTCC CCCGCGGCCG GCAGCTCCCG GGGCGTCTGG 
     1141 GCTGCCTGCT CGAGGAGGGC CTCTGCGGAG CGTCCGAGGC CTGTGTGAAC GATGGAGTGT 
     1201 TTGGAAGGTG CCAGAAGGTT CCGGCAATGG ACTTTTACCG CTACGAGGTG TCGCCCGTGG 
     1261 CCCTGCAGCG CCTGCGCGTG GCGTTGCAGA AGCTTTCCGG CACAGGTTTC ACGTGGCAGG 
     1321 ATGACTATAC TCAGTATGTG ATGGACCAGG AACTTGCAGA CCTCCCGAAA ACCTACCTGA 
     1381 GGCGTCCTGA AGCATCCAGC CCAGCCAGGC CCTCAAAACA CAGCGTTGGC AGCGAGAGGA 
     1441 GGTACAGTCG GGAGGGCGGT GCTGCCCTGG CCAACGCCCT CCGACGCCAC CTGCCCTTCC 
     1501 TGGAGGCCCT GTCCCAGGCC CCAGCCTCAG ACGTGCTCGC CAGGACCCAT ACGGCGCAGG 
     1561 ACAGACCCCC CGCTGAGGGT GATGACCGCT TCTCCGAGAG CATCCTGACC TATGTGGCCC 
     1621 ACACGTCTGC GCTGACCTAC CCTCCCGGGT CCCGGACCCA GCTCCGCGAG GACCTCCTGC 
     1681 CGCGGACCCT CGGCCAGCTC CAGCCAGATG AGCTCAGCCC TAAGGTGGAC AGTGGTGTGG 
     1741 ACAGACACCA TCTGATGGCG GCCCTCAGTG CCTATGCTGC CCAGAGGCCC CCAGCTCCCC 
     1801 CCGGGGAGGG CAGCCTGGAG CCACAGTACC TTCTGCGTGC ACCCTCAAGA ATGCCCAGGC 
     1861 CTTTGCTGGC ACCAGCCGCC CCCCAGAAGT GGCCTTCACC TCTGGGAGAT TCCGAAGACC 
     1921 CCTCCAGCAC AGGCGATGGA GCACGGATTC ATACCCTCCT GAAGGACCTG CAGAGGCAGC 
     1981 CGGCTGAGGT GAGGGGCCTG AGTGGCCTGG AGCTGGACGG CATGGCTGAG CTGATGGCTG 
     2041 GCCTGATGCA AGGCGTGGAC CATGGAGTAG CTCGAGGCAG CCCTGGGAGA GCGGCCCTGG 
     2101 GAGAGTCTGG AGAACAGGCG GATGGCCCCA AGGCCACCCT CCGTGGAGAC AGCTTTCCAG 
     2161 ATGACGGAGT GCAGGACGAC GATGATAGAC TTTACCAAGA GGTCCATCGT CTGAGTGCCA 
     2221 CACTCGGGGG CCTCCTGCAG GACCACGGGT CTCGACTCTT ACCTGGAGCC CTCCCCTTTG 
     2281 CAAGGCCCCT CGACATGGAG AGGAAGAAGT CCGAGCACCC TGAGTCTTCC CTGTCTTCAG 
     2341 AAGAGGAGAC TGCCGGAGTG GAGAACGTCA AGAGCCAGAC GTATTCCAAA GATCTGCTGG 
     2401 GGCAGCAGCC GCATTCGGAG CCCGGGGCCG CTGCGTTTGG GGAGCTCCAA AACCAGATGC 
     2461 CTGGGCCCTC GAAGGAGGAG CAGAGCCTTC CAGCGGGTGC TCAGGAGGCC CTCAGCGACG 
     2521 GCCTGCAATT GGAGGTCCAG CCTTCCGAGG AAGAGGCGCG GGGCTACATC GTGACAGACA 
     2581 GAGACCCCCT GCGCCCCGAG GAAGGAAGGC GGCTGGTGGA GGACGTCGCC CGCCTCCTGC 
     2641 AGGTGCCCAG CAGTGCGTTC GCTGACGTGG AGGTTCTCGG ACCAGCAGTG ACCTTCAAAG 
     2701 TGAGCGCCAA TGTCCAAAAC GTGACCACTG AGGATGTGGA GAAGGCCACA GTTGACAACA 
     2761 AAGACAAACT GGAGGAAACC TCTGGACTGA AAATTCTTCA AACCGGAGTC GGGTCGAAAA 
     2821 GCAAACTCAA GTTCCTGCCT CCTCAGGCGG AGCAAGAAGA CTCCACCAAG TTCATCGCGC 
     2881 TCACCCTGGT CTCCCTCGCC TGCATCCTGG GCGTCCTCCT GGCCTCTGGC CTCATCTACT 
     2941 GCCTCCGCCA TAGCTCTCAG CACAGGCTGA AGGAGAAGCT CTCGGGACTA GGGGGCGACC 
     3001 CAGGTGCAGA TGCCACTGCC GCCTACCAGG AGCTGTGCCG CCAGCGTATG GCCACGCGGC 
     3061 CACCAGACCG ACCTGAGGGC CCGCACACGT CACGCATCAG CAGCGTCTCA TCCCAGTTCA 
     3121 GCGACGGGCC GATCCCCAGC CCCTCCGCAC GCAGCAGCGC CTCATCCTGG TCCGAGGAGC 
     3181 CTGTGCAGTC CAACATGGAC ATCTCCACCG GCCACATGAT CCTGTCCTAC ATGGAGGACC 
     3241 ACCTGAAGAA CAAGAACCGG CTGGAGAAGG AGTGGGAAGC GCTGTGCGCC TACCAGGCGG 
     3301 AGCCCAACAG CTCGTTCGTG GCCCAGAGGG AGGAGAACGT GCCCAAGAAC CGCTCCCTGG 
     3361 CCGTGCTGAC CTATGACCAC TCCCGGGTCC TGCTGAAGGC GGAGAACAGC CACAGCCACT 
     3421 CAGACTACAT CAACGCTAGC CCCATCATGG ATCACGACCC GAGGAACCCC GCGTACATCG 
     3481 CCACCCAGGG ACCGCTGCCC GCCACCGTGG CTGACTTTTG GCAGATGGTG TGGGAGAGCG 
     3541 GCTGCGTGGT GATCGTCATG CTGACACCCC TCGCGGAGAA CGGCGTCCGG CAGTGCTACC 
     3601 ACTACTGGCC GGATGAAGGC TCCAATCTCT ACCACATCTA TGAGGTGAAC CTGGTCTCCG 
     3661 AGCACATCTG GTGTGAGGAC TTCCTGGTGA GGAGCTTCTA TCTGAAGAAC CTGCAGACCA 
     3721 ACGAGACGCG CACCGTGACG CAGTTCCACT TCCTGAGTTG GTATGACCGA GGAGTCCCTT 
     3781 CCTCCTCAAG GTCCCTCCTG GACTTCCGCA GAAAAGTAAA CAAGTGCTAC AGGGGCCGTT 
     3841 CTTGTCCAAT AATTGTTCAT TGCAGTGACG GTGCAGGCCG GAGCGGCACC TACGTCCTGA 
     3901 TCGACATGGT TCTCAACAAG ATGGCCAAAG GTGCTAAAGA GATTGATATC GCAGCGACCC 
     3961 TGGAGCACTT GAGGGACCAG AGACCCGGCA TGGTCCAGAC GAAGGAGCAG TTTGAGTTCG 
     4021 CGCTGACAGC CGTGGCTGAG GAGGTGAACG CCATCCTCAA GGCCCTTCCC CAGAGCGGAC 
     4081 CGACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4141 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4201 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4261 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4321 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4381 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4441 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4501 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4561 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4621 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4681 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4741 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     4801 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     4861 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     4921 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     4981 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5041 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5101 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5161 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5221 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5281 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5341 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5401 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5461 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5521 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5581 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     5641 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     5701 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     5761 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     5821 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     5881 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     5941 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6001 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6061 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6121 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6181 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6241 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6301 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6361 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6421 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6481 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6541 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6601 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6661 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     6721 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     6781 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     6841 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     6901 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     6961 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7021 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7081 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7141 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7201 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7261 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7321 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7381 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7441 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7501 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7561 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7621 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7681 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     7741 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     7801 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     7861 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     7921 TTTAACGCGA ATTTT
//